• Title/Summary/Keyword: N-R Method

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Development and evaluation of surface plasmon resonance imaging for the detection of antibodies against classical swine fever virus in swine

  • Cho, Ho-Seong;Lee, Tae-Uk;Park, Nam-Yong
    • Korean Journal of Veterinary Service
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    • v.30 no.2
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    • pp.205-209
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    • 2007
  • A protein chip based on surface plasmon resonance (SPR) imaging was developed for measuring classical swine fever virus (CSFV) antibody using a recombinant gp55 protein as an antigen. The diagnostic potential of SPR imaging for detecting antibodies to the CSFV gp55 protein was compared with that of a enzyme -linked immunosorbent assay (ELISA) using 70 pig sera. There was a strong positive correlation between the SPR imaging and ELISA (n=70, r=0.916, p<0.01). Therefore, the SPR imaging, which is a label-free and high-through put method, is expected to be a valuable tool in the serodiagnosis of CSFV.

Purification of the Vacuolar Arginine Transporter from Neurospora crassa (Neurospora crassa로부터 arginine transporter의 순수분리)

  • ;Weiss, R. L.
    • Korean Journal of Microbiology
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    • v.27 no.2
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    • pp.117-123
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    • 1989
  • Radioactive N-$\alpha$-p-nitrobenzoxycarbonyl (NBZ)-L-[2,$3-^{3}$H] arginyl diazomethane was used as an affinity label for the vacuolar arginine transporter in Neurospora crassa. Vacuolar matrix proteins were removed by fracturing the membranes with freeze-thaw method in dry ice/ethanol bath. Vacuolar membrane proteins were then wasged with 500mM NaCl to remove ionically bound derivatives and peripheral membrane proteins from vacuolar membranes. After dissolved in 1% Titon X-100, dissolved vacuolar memvrane proteins were separated with molecular sieve column chromatography, anion and cation exchange chromatographies. The arginine transporter was purified giving the purification factor of 1136.

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Electro-spun Antimicrobial Acrylic Fiber

  • Lee, Jae-Woong;Ren, Xue-Hong;Broughton, R.M.;Liang, Jie;Worley, S.D.;Huang, T.S.
    • Textile Coloration and Finishing
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    • v.19 no.2
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    • pp.44-49
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    • 2007
  • Antimicrobial fibers were prepared by an electro-spinning method. Polystyrene hydantoin(PSH) was employed as an antimicrobial precursor to produce an electro-spun antimicrobial acrylic fiber. Increasing the surface area of hydrophobic antimicrobial-fibers provides enhanced antimicrobial efficacy. The biocidal activity of electro-spun acrylic fibers could be rendered through chlorine bleach treatment, and the antimicrobial effectiveness against gram-Positive and gram-negative bacteria was investigated. In addition, scanning electron microscopy(SEM) demonstrated the feature of the electro-spun fibers.

DEVELOPMENT AND VALIDATION OF A NUCLEAR FUEL CYCLE ANALYSIS TOOL: A FUTURE CODE

  • Kim, S.K.;Ko, W.I.;Lee, Yoon Hee
    • Nuclear Engineering and Technology
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    • v.45 no.5
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    • pp.665-674
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    • 2013
  • This paper presents the development and validation methods of the FUTURE (FUel cycle analysis Tool for nUcleaR Energy) code, which was developed for a dynamic material flow evaluation and economic analysis of the nuclear fuel cycle. This code enables an evaluation of a nuclear material flow and its economy for diverse nuclear fuel cycles based on a predictable scenario. The most notable virtue of this FUTURE code, which was developed using C# and MICROSOFT SQL DBMS, is that a program user can design a nuclear fuel cycle process easily using a standard process on the canvas screen through a drag-and-drop method. From the user's point of view, this code is very easy to use thanks to its high flexibility. In addition, the new code also enables the maintenance of data integrity by constructing a database environment of the results of the nuclear fuel cycle analyses.

RF Loss Minimization Method Using High Impedance Filter for Research (High Impedance Filter를 이용한 RF Loss 최소화 방법에 대한 연구)

  • Wang, Hyun-Chul;Seo, Hwa-Il
    • Journal of the Semiconductor & Display Technology
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    • v.19 no.1
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    • pp.55-60
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    • 2020
  • This study designed High impedance filter to reduce RF loss to heater heating wire and increase RF current flowing to heater ground wire. Effects such as D / R improvement and process reproducibility could be seen. In addition, RF parameter distribution optimization was possible by understanding the RF path of PE-CVD equipment using Plasma and designing filter.

Effects of Spiritual Well-being on Spiritual Nursing Intervention (간호사의 영적간호수행에 대한 영적안녕의 예측정도)

  • Sung, Mi-Hae
    • Asian Oncology Nursing
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    • v.9 no.1
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    • pp.15-22
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    • 2009
  • Purpose: The purpose of this study was to identify the relationship between spiritual well-being and spiritual nursing intervention among nurses. Method: Participants (N=171) were recruited from September to November 2007. Data were analyzed using descriptive statistics, t-test, ANOVA, Pearson's correlation and multiple regression. Results: The score of spiritual well-being of nurses was 2.70 out of a total score of 4 and that of spiritual nursing care was 2.31 out of a total score of 4. There was a significantly positive relationship between spiritual well-being and spiritual nursing care (r=0.281, p<0.001). Spiritual well-being and clinical career explained 12.1% of the variance in spiritual nursing care of nurses. Conclusion: Spiritual well-being has the most important influence on spiritual nursing intervention of nurses. The findings suggest that it is needed to develop effective programs to improve spiritual well-being of nurses. More studies are required to identify other predictors of spiritual nursing intervention.

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Effective Clustering Method for High-Dimensional Indexes (고차원 색인을 위한 효과적 클러스터링 기법)

  • 신봉근;곽태영;최승락;이윤준;김명호
    • Proceedings of the Korean Information Science Society Conference
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    • 1998.10b
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    • pp.247-249
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    • 1998
  • 최근 들어 내용기반의 이미지 검색을 지원하기 위한 방법으로, 특징 벡터를 이용한 유사 질의 연구가 활발히 진행되고 있다. 이러한 유사 질의를 효율적으로 지원하기 위해서는 고차원 공간상에 존재하는 점 데이터나 공간 데이터를 효과적으로 색인할 수 있는 색인 기법이 필요하다. 하지만 R*-트리를 바탕으로 하는 기존의 방법들은 고차원 데이터에 대해서 차원의 증가함에 따라 검색 시간이 급격하게 증가하는 문제점을 안고 있다. 이러한 문제는 데이터의 클러스터링에 기반을 둔 기존의 방법들이 차원이 증가함에 따라 데이터를 제대로 클러스터링하지 못하기 때문에 발생하며, 따라서 이를 해결하기 위해서는 효과적인 클러스터링 기법이 필요하다. 본 논문에서는 하나의 최소 한계 영역(minimum bounding region)에 속하는 개체들의 응집 정도와 최소 한계 영역들간의 결합 정도를 고려하여 효과적으로 클러스터링하는 방안을 제안한다. 또한 이러한 클러스터링 기법을 수용하기 위한 색인 기법을 간략히 제시한다

Volatile Flavor Components of Leek(Allium tuberosum Rottler) (부추(Allium tuberosum Rottler)의 휘발성 향기성분)

  • 김경수;박은령;조정옥;김선민;이명렬
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.27 no.4
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    • pp.563-567
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    • 1998
  • Volatile flavor components of edible portion of leek(Allium tuberosum R.) were extracted by SDE(simultaneous steam distillation and extraction) method using the mixture of n-pentane and diethylether (1 : 1, v/v) as an extract solvent and analyzed by GC-FID and GC/MS. Identification of the volatile flavor components was mostly based on the RI of GC and mass spectrum of GC/MS. A total of sixty-five components from leek extract were classified as 28 sulfur-containing compounds, 12 aldehydes, 9 alcohols, 4 lactones and esters, 3 acids and hydrocarbons, and 2 miscellaneous compouds. The sulfur-containing compounds were predominant in leek extract. Dimethyl disulfide(19.47%) and dimethyl trisulfide(17.38%) were the main compounds and trans-1-propenyl methyl disulfide, trans-2-hexenal and methyl allyl disulfide were also detected large amounts in leek.

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A Numerical Simulation for Contractive and Dilative Periodic Motion on Axisymmetric Body

  • Kim, Moon-Chan
    • Journal of Ship and Ocean Technology
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    • v.3 no.1
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    • pp.1-11
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    • 1999
  • Numerical simulation for the axisymmetric body with contractive and dilative periodic motion is carried out. The present analysis shows that a propulsive force can be obtained in highly viscous fluid by the contractive and dilative motion of axisymmetric body. An axisymmetric code is developed with unstructured grid system for the simulation of complicated motion and geometry. It is validated by comparing with the results of Stokes approximation with the problem of uniform flow past a sphere in low Reynolds number($R_n$ = 1). The validated code is applied to the simulation of contractive and dilative periodic motion of body whose results are quantitatively compared with the two dimensional case. The simulation is extended to the analysis of waving surface with projecting part for finding out the difference of hydrodynamics performance according to variation of waving surface configuration. The present study will be the basic research for the development of the propulsor of an axisymmetric micro-hydro-machine.

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A Study on the Relations Between Physical Exercise and Quality of Life in Middle-aged Adults (중년기 성인의 운동실천과 삶의 질)

  • Han Su Jeong
    • Journal of Korean Public Health Nursing
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    • v.18 no.1
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    • pp.14-26
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    • 2004
  • Purpose: This study was done to identify the relationship between physical exercise and quality of life in middle-aged adults. Method: The subjects of this study consisted of 251 mid-aged adults who lived in D city and N city. The instrument for this study was exercise performance scale by Kim and QOL scale by Ro. Data were collected form February 20 to March 20. 2003. Result: 1. Among participants. 'good' exercise performance consisted $39.2\%$ and exercise involvement was $41\%$. 2. The quality of life showed positive significant correlation with the exercise performance(r=.21. p=.00). 3. Exercise performance was a predictor of quality of life and $8.3\%$ of the variance explained with exercise performance. Conclusion: The results of this study suggest that further development and applying of exercise performance programs are recommended for quality of life of mid-aged adults. In addition. further research needs to be done to identify relationship with these factors for various age group.

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