• Proceedings of the Korea Water Resources Association Conference
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• 2021.06a
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• pp.304-304
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• 2021

도시화가 진행됨에 따라 불투수지역이 늘어나는 등 개발전과 다른 지표 및 지표하 유출이 나타나며, 이러한 변화는 생태적, 수문학적으로 대규모 변화가 나타나는 것을 의미한다. 이에 따라 물관리, 물순환 등 물 지속 가능성은 대중의 관심을 받아 법, 제도가 변화하며 도시화에 대한 세심한 접근과 관리에 대한 계획이 대두되고 있는 실정이다. 저영향개발(Low Impact Development)은 이러한 도시화로 인해 발생될 수 있는 문제를 해결하기 위한 방안의 하나로 제안되고 있으며 첨두 및 유출총량을 완화하여 개발 이전 상태의 수문학적 기능을 유지하거나 물리학 및 생물학적 기작을 통해 오염물질의 저감효과도 나타나 대규모 도시개발 및 유역관리 사업에서 저영향개발기법의 적용이 점차 늘어나고 있는 추세이다. 그러나 저영향개발기법 설치에 관한 구체적인 매뉴얼 또는 가이드라인이 부족한 실정이기 때문에 저영향개발기법이 계획단계에서 양적으로 무분별하게 적용되어 비용적 측면에서 시공자에게 부담을 가중시키는 등의 문제가 발생한다. 또한 시설의 위치에 따라 강우유출수 및 비점오염원 저감에 대한 효과가 상이하기 때문에 시설의 적정 위치선정 및 시설용량, 경제성을 고려한 시설효과 검토 등 저영향개발기법 적용시 사업부지 특성에 따른 최적화를 고려하여 계획 및 설계에 적용할 필요성이 있다. 이에 비용적 측면을 고려한 최적설계가 가능한 SWMM-ING(Storm Water Management Model - Low impact development desigN proGram)을 개발된 바 있다. SWMM-ING은 비전문가도 사용이 가능하도록 저영향개발기법 최적화과정이 간단하고 직관적이며 비용을 고려한 최적설계가 가능하도록 개발된 소프트웨어로 기법 적용시 개략적인 비용추정 및 배치선정 등 저영향개발기법의 설계최적화가 가능하다. 또한 각각의 저영향개발기법에 대한 초기설치비용, 유지관리비용, 이자율, 기대수명, 대상지역 내 공간적 위치를 반영하여 강우유출수 및 비점오염저감효과를 시나리오별로 제시가 가능한 장점이 있다. 본 연구에서는 SWMM-ING를 이용하여 대규모 도시개발부지의 저영향개발기법 적용에 따른 유량 및 수질저감효과를 평가하고 유지관리비를 포함한 비용을 추정하여 최적설계 검토를 수행하였다.

• International Journal of Computer Science & Network Security
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• v.23 no.7
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• pp.186-192
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• 2023

Malwares are becoming a major problem nowadays all around the world in android operating systems. The malware is a piece of software developed for harming or exploiting certain other hardware as well as software. The term Malware is also known as malicious software which is utilized to define Trojans, viruses, as well as other kinds of spyware. There have been developed many kinds of techniques for protecting the android operating systems from malware during the last decade. However, the existing techniques have numerous drawbacks such as accuracy to detect the type of malware in real-time in a quick manner for protecting the android operating systems. In this article, the authors developed a hybrid model for android malware detection using a decision tree and KNN (k-nearest neighbours) technique. First, Dalvik opcode, as well as real opcode, was pulled out by using the reverse procedure of the android software. Secondly, eigenvectors of sampling were produced by utilizing the n-gram model. Our suggested hybrid model efficiently combines KNN along with the decision tree for effective detection of the android malware in real-time. The outcome of the proposed scheme illustrates that the proposed hybrid model is better in terms of the accurate detection of any kind of malware from the Android operating system in a fast and accurate manner. In this experiment, 815 sample size was selected for the normal samples and the 3268-sample size was selected for the malicious samples. Our proposed hybrid model provides pragmatic values of the parameters namely precision, ACC along with the Recall, and F1 such as 0.93, 0.98, 0.96, and 0.99 along with 0.94, 0.99, 0.93, and 0.99 respectively. In the future, there are vital possibilities to carry out more research in this field to develop new methods for Android malware detection.

• Microbiology and Biotechnology Letters
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• v.51 no.4
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• pp.390-402
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• 2023

Salmonella are Gram-negative pathogenic bacteria commonly found in food animals such as poultry and swine and potentially constitute risks and threats to food safety and public health through transmissible virulence and antimicrobial resistance (AMR) genes. Although there are previous studies in the Philippines regarding genotypic and phenotypic AMR in Salmonella, there are very few on virulence and their associations. Hence, this study collected 700 Salmonella isolates from swine samples in abattoirs and wet markets among four districts in Metro Manila and characterized their genotypic virulence and β-lactam AMR profiles. Gene frequency patterns and statistical associations between virulence and bla genes and comparisons based on location types (abattoirs and wet markets) and districts were also determined. High prevalence (>50%) of virulence genes was detected encompassing Salmonella pathogenicity islands (SPIs) 1-5 suggesting their pathogenic potential, but none possessed plasmid-borne virulence genes spvR and spvC. For bla, bla_TEM was detected with high prevalence (>45%) and revealed significant associations to four SPI genes, namely, avrA, hilA, mgtC, and spi4R, which suggest high resistance potential particularly to β-lactam antibiotics and relationships with pathogenicity that remain mechanistically unestablished until now. Lastly, comparisons of location types and districts showed variations in gene prevalence suggesting effects from environmental factors throughout the swine production chain. This study provides vital data on the genotypic virulence and AMR of Salmonella from swine in abattoirs and wet markets that suggest their pathogenicity and resistance potential for policymakers to implement enforced surveillance and regulations for the improvement of the Philippine swine industry.

• Korean Journal of Soil Science and Fertilizer
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• v.19 no.2
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• pp.157-165
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• 1986

This experiment was conducted to find out the some physiological characteristics and nitrogen fixation activities under free-living conditions of indigenous rhizobia isolated from soybean-cultivated (30 fields) and un-cultivated (30 fields) soil. The results were summerized as follows: 1. In free-living condition, only 12.8% and 6.4% of the indigenous rhizobia isolated from soybean cultivated (133 strains) and un-cultivated (125 strains) soils were nitrogenase positive as more than 4 n mole $C_2H_4$ per tube per hour by acetylene reduction assay. 2. The acid-producing rhizobia on litmus milk test was observed to be 20% of the total 160 strains isolated from soybean cultivated soil but about 34% of 166 strains isolated from un-cultivated soil. And the serum zone positive strains were higher in the soybean un-cultivated soil than cultivated soil. 3. The population ratio of fast-to slow-growing indigenous rhizobia based on growth pattern of AMA medium was 35.6% to 64.4% of the total 346 strains. 4. The population of indigenous Rhizobium japonicum counted by MPN method was ranged from $9.2{\times}10^2$ cells per gram of soil in soybean un-cultivated soil to $2.3{\times}10^4$ cells per gram of soil soybean cultivated soil. The number of indigenous R. japonicum in 0-10cm depth of surface layer was higher than low layer.

• Biomedical Science Letters
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• v.8 no.3
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• pp.115-125
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• 2002

Gram-negative septicemia, which continues to be a serious clinical problem, is one of the major causes of morbidity and mortality in hospitalized patients. Endotoxin of gram-negative bacteria is a pivotal pathogen of sepsis. To understand the effect of endotoxin on hematological aspect and the time course in early childhood, this study was designed with experimental septic model of young rabbits (8 week-old). Rabbits were divided into control (n=7) and endotoxin group (0.50 mg/kg of endotoxin). The endotoxin group was subdivided into six groups by the sampling times: 3, 6, 12, 24, 48 and 72 hr-group (E-G$_{3}$, E-G$_{6}$, E-G$_{12}$, E-G$_{24}$, E-G$_{48}$ and E-G$_{72hrs}$, each n=7). The evaluation of CBC, activated partial thromboplastin time (APTT), prothrombin time (PT), fibrinogen concentration, coagulation factors and D-dimer were taken from the bloods. The number of leukocytes was lower in E-G$_{3}$ and E-G$_{6hrs}$ (due to pantocytopenia), whereas it was higher in E-G$_{24}$ and E-G$_{48}$ (due to neutrophilia and/or lymphophilia) than in control group (P<0.05). Platelet counts in E-G$_{3}$, E-G$_{6}$, E-G$_{12}$, E-G$_{24}$ and E-G$_{48hrs}$ were lower than those of control group (P<0.05). Normoblast counts in E-G$_{3}$, E-G$_{6}$, E-G$_{12}$, E-G$_{24}$ and E-G$_{48hrs}$ were higher than those of control group (p<0.01). APTT in E-G$_{3}$, E-G$_{6}$, E-G$_{12}$, E-G$_{24}$ and E-G$_{72hrs}$ were longer while PT in E-G$_{3}$, E-G$_{6}$, E-G$_{48}$ and E-G$_{72hrs}$ were higher than those of control group (p<0.05). Fibrinogen concentrations were lower in E-G$_{3}$, E-G$_{6}$ and E-G$_{12}$ but higher in E-G$_{48}$ and E-G$_{72hrs}$ than those of control (p<0.05). Intrinsic coagulation factors (XII, XI, IX, VIII) in all endotoxin groups were significantly lower than those of control group (p<0.05). Extrinsic coagulation factor (X, VII, V, II) were lower in E-G$_{3}$, E-G$_{6}$, E-G$_{12}$ and E-G$_{24hrs}$ whereas they were higher in E-G$_{48}$ and E-G$_{72hrs}$ than in control group (p<0.05). D-dimer concentrations in E-G$_{48}$ and E-G$_{72hrs}$ were higher than those of control group (P<0.001). We concluded that endotoxin led to extensive hematological disturbances including disseminated intravascular coagulation in the young rabbits and that this pathologic condition in the infant and childhood groups will cause the grave results.

• Korean Journal of Environmental Agriculture
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• v.9 no.2
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• pp.83-95
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• 1990

This study was conducted to estimate influences of pesticides such as carbofuran[2,3-dihydro-2,2-dimethylbenzofuran-7-yl methyl carbamate] as an insecticide, and pyrazolate [4-(2,4-dichlorobenzolyl)-1,3-dimethyl-5-pyrazolyl-1,3-dimethyl-5-pyrazolyl-p-toluensulfonate], pyrazolate+pretilachlor [2-chlor-2,6-diethyl-N-(n-propoxyethyl) acetanilied] as herbicides on change in numbers of soil microorganisms and pH in planted and unplanted flooded rice paddy soils. The results of weekly investigated change of pH and populations of total bacteria, gram negative bacteria, anaerobic bacteria and fungi after treatments of pesticides were as follows : The change of pH in rice-planted soil gradually decreased in a matter of weeks after treatment with pesticide and the pH increased again from the sixth week, but no change of pH could be observed in nonplanted soil. The total numer of bacteria in the treated plots were slightly less than in the control plot, and the numbers decreased with increasing application rates of pesticides. But the microbial population increased in a matter of days after treatment with pesticide. Number of the gram negative bacteria until the sixth week after treatment of pesticide were fewer than control. The number in the carbofuran-treated plot decreased after a weeks after treatment, but numbers in plots treated with pyrazolate and pyrazolate+pretilachlor increased. The number of anaerobic bacteria in the treated plots were few by comparison with the untreated control, but the number increased after a weeks after treatment with pesticides. The populations of fungi in the carbofuran-treated plot were similar by comparison with the untreated control. The populations in the plots treated with pyrazolate and pyrazolate+pretilachlor decreased in 4 to 5 weeks with increase of application rate, but afterwards increased.

• Korean Journal of Soil Science and Fertilizer
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• v.37 no.3
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• pp.177-183
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• 2004

The discharge of waste nutrient solution from greenhouse to natural ecosystem leads to the accumulation of excess nutrients that results in contamination or eutrophication. There is a need to recycle the waste nutrient solution in order to prevent the environmental hazards. The amount and kind of nutrients in waste nutrient solution might be enough to grow photosynthetic microorganisms. Hence in the present study, we examined the growth and mass cultivation of cyanobacteria in the waste nutrient solution with an objective of removing N and P and concomitantly, its mass cultivation. Four photosynthetic filamentous cyanobacteria (Anabaena HA101, HA701 and Nostoc HN601, HN701) isolated from composts and soils of the Chungnam province were used as culture strains. Among the isolates, Nostoc HN601 performed faster growth rate and higher N and P uptake in the BG-II ($NO_3{^-}$) medium when compared to those of other cyanobacterial strains. Finally, the selected isolate was tested under optimum conditions (airflow at the rate of $1L\;min^{-1}$. in 15 L reactor, initial pH 8) in waste nutrient solution from tomato hydroponic in green house condition. Results showed to remove 100% phosphate from the waste nutrient solution in the tomato hydroponics recorded over a period of 7 days. The growth rate of Nostoc HN601 was $16mg\;Chl-a\;L^{-1}$ in the waste nutrient solution from tomato hydroponics with optimum condition, whereas growth rate of Nostoc HN601 was only $9.8mg\;Chl-a\;L^{-1}$ in BG-11 media. Nitrogen fixing capacity of Nostoc HN601 was $20.9nmol\;C_2H_4\;mg^{-1}\;Chl-a\;h^{-1}$ in N-free BG-11. The total nitrogen and total phosphate concentration of Nostoc HN601 were 63.3 mg N gram dry weight $(GDW)^{-1}$ and $19.1mg\;P\;GDW^{-1}$ respectively. Collectively, cyanobacterial mass production using waste nutrient solution under green house condition might be suitable for recycling and cleaning of waste nutrient solution from hydroponic culture system. Biomass of cyanobacteria, cultivated in waste nutrient solution, could be used as biofertilizer.

• Korean Journal of Microbiology
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• v.48 no.3
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• pp.180-186
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• 2012

Pseudomonas aeruginosa, a Gram-negative bacterium, is an ubiquitous and opportunistic human pathogen, which expresses many virulence factors through quorum sensing (QS) regulation. QscR, one of the QS signal receptors of P. aeruginosa, has unique features that make it possible to distinguish QscR from other QS receptors. In the present study, we focused on amino acid residues responsible for such a broad signal specificity of QscR. Thus we constructed mutant QscRs: $QscR_{T72I}$, $QscR_{R132M}$, and $QscR_{T140I}$ by substituting $72^{nd}$ threonine, $132^{nd}$ arginine, and $140^{th}$ threonine residues with isoleucine, methionine, and isoleucine, respectively by site-directed mutagenesis. When we examined the activity of these mutant QscRs, $QscR_{R132M}$ failed to respond to N-3-oxododecanoyl homoserine lactone (3OC12-HSL), but $QscR_{T72I}$ and $QscR_{T140I}$ remained the ability to respond to 3OC12-HSL despite much reduction of the sensitivity. When we treated a variety of acyl-HSLs with different structure, $QscR_{T72I}$ and $QscR_{T140I}$ showed better responsiveness to N-decanoyl HSL (C10-HSL) or N-dodecanoyl HSL (C12-HSL) that has no oxo-moiety at $3^{rd}$ carbon of acyl group than to 3OC12-HSL, and $QscR_{R132M}$ showed no responsiveness to any acyl-HSLs tested here. In addition, $QscR_{T72I}$ and $QscR_{T140I}$ were inhibited by 5f, a QscR inhibitor as similarly as wild type QscR was. These results suggest that while the $130^{th}$ arginine is crucial in both activity and acyl-HSL binding of QscR, the $72^{nd}$ and $140^{th}$ threonines are important in the activity, but they are little responsible for the discrimination of acyl-HSLs or competitive inhibitor.

• Journal of Internet Computing and Services
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• v.18 no.6
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• pp.35-46
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• 2017

Recently, attackers using malicious code in cyber security have been increased by attaching malicious code to a mail and inducing the user to execute it. Especially, it is dangerous because it is easy to execute by attaching a document type file. The author analysis is a research area that is being studied in NLP (Neutral Language Process) and text mining, and it studies methods of analyzing authors by analyzing text sentences, texts, and documents in a specific language. In case of attack mail, it is created by the attacker. Therefore, by analyzing the contents of the mail and the attached document file and identifying the corresponding author, it is possible to discover more distinctive features from the normal mail and improve the detection accuracy. In this pager, we proposed IADA2(Intelligent Attack mail Detection based on Authorship Analysis) model for attack mail detection. The feature vector that can classify and detect attack mail from the features used in the existing machine learning based spam detection model and the features used in the author analysis of the document and the IADA2 detection model. We have improved the detection models of attack mails by simply detecting term features and extracted features that reflect the sequence characteristics of words by applying n-grams. Result of experiment show that the proposed method improves performance according to feature combinations, feature selection techniques, and appropriate models.

• Korean Journal of Clinical Laboratory Science
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• v.50 no.2
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• pp.85-92
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• 2018

Information on the prevalence of S. aureus and the current antimicrobial resistance profile is necessary in selecting the appropriate treatment of S. aureus infections in any part of the world. This study examined the frequency and antibiotic resistance list of S. aureus isolates obtained from clinical specimens at one hospital in Korea. A total of 1,746 gram positive cocci collected were identified as S. aureus. S. aureus isolates were obtained from different samples including sputum (N=565; 32.4%), endotracheal aspirate (358; 20.5%), wounds (329; 18.8%), blood (137; 7.8%), urine (67; 3.8%), and pus (59; 3.4%). All 1,545 S. aureus (100%) strains screened from sputum (565; 36.6%), endotracheal aspirate (388; 25.1%), wounds (329; 21.3%), blood (137; 8.9%), urine (67; 4.3%), and pus (59; 3.8%) were sensitive to glycopeptide (vancomycin, teicoplanin), oxazolidinone (linezolid) and stretogramin (quinupristin/dalfopristin). The prevalence of resistant S. aureus was significantly (P<0.01) lower in urine, blood, pus, wounds, and sputum than in endotracheal aspirates. As a result, there was a significant difference in the antibiotic resistance of S. aureus according to the clinical specimens.