• Korean Journal of Ecology and Environment
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• v.41 no.4
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• pp.554-562
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• 2008

To evaluate the limitation for epiphytic and planktonic algal growth, acid extractable inorganic phosphorus (AP), implying the luxury uptake phosphorus, was measured in five reed zones of Lake Biwa. The AP in epiphytic substances was 0.7 to 1.4 mg P surface stem $m^{-2}$ in summer and 1.2 to 2.8 mg P $m^{-2}$ in winter. On the other hand, the amount in planktonic substances was 1.4 to 5.7 mg P m -3 and 0.8 to 5.4 mg P $m^{-3}$ in both seasons. Contribution of AP in the epiphytic and planktonic phosphorus was 23 to 31% and 8 to 27% in summer, and 17 to 22% and 9 to 17% in winter. It suggests that in summer both epiphytic and planktonic algae had been luxuriously taken up phosphate into cells. The weight ratios of C : N : P were averaged 79 : 20 : 1 for the epiphytic substances and 81 : 12 : 1 for the particulate substances. On the other hand, the ratios without the luxurious phosphorus were 93 : 24 : 1 and 103 : 15 : 1, showing much higher values than the Redfield ratio. High ratio in the epiphytic substances indicates that the phosphorus is the limiting parameter, rather than nitrogen, regulating the growth of epiphytic algal populations.

• Journal of Korean Society of Environmental Engineers
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• v.27 no.4
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• pp.431-437
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• 2005

In this study, the profiles of the bacterial regrowth of indigenous bacteria in tap water and Pseudomonas fluorescence P17 were investigated for cases when carbon (glucose), and/or nitrogen ($NO_3^-$-N), and/or phosphorus ($PO_4^{3-}$-P) were added below sufficient nutrient concentration (SNC) and when carbon sources (glucose and acetate) and nitrogen sources ($NH_4^+$-N and $NO_3^-$-N) were added together. The bacterial regrowth was decreased with limitation of nutrients, and were lowered relatively in the sample, which plural nutrients were limited. In addition, phosphate might be the effective nutrient to control the bacterial regrowth in drinking water because the bacterial regrowth was significantly decreased by the limitation of phosphate. In contrast, the bacterial regrowth was retarded with increasing the concentration of $NO_3^-$-N. For simultaneously adding carbon(glucose or acetate) and nitrogen sources ($NH_4^+$-N and $NO_3^-$-N), the regrowth counts appeared highly in the condition, for both glucose and acetate. And, the regrowth was increased with increasing $NH_4^+$-N concentration as a nitrogen source.

• Journal of Microbiology and Biotechnology
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• v.7 no.6
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• pp.391-396
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• 1997

Two series of carbon sources, linear primary $C_1$~$C_9$ alcohols and linear $C_2$~$C_{10}$ monocarboxylic acids were tested for PHA synthesis in Paracoccus denitrificans. The results showed that the growth-associated synthesis of PHA could be referred only to the carbon sources with odd number of carbon except methanol. For all carbon sources with even number of carbon, nitrogen limitation was required to induce PHA synthesis in P. denitrificans. Poly(3-hydroxyvalerate)[P(3HV)] homopolymer was synthesized from $C_5$, $C_7$, and $C_9$ while growing in the presence of nitrogen, but the nitrogen depletion in the later growth period incorporated 3-hydroxybutyrate(3HB) unit into the polymer chain. The optimum C/N ratio for P(3HV) homopolymer production was found to be 10 when the strain was grown on 10 ml/l of valeric acid for 96 h. P. denitrificans synthesized P(3HB-co-3HV) copolymers from n-hexanoic and n-octanoic acid. The microstructural characterics of the P(3HB-co-3HV) copolymer from n-propanol was investigated using $^13C$-nuclear magnetic resonance spectroscopy, showing a structural heterogeneity.

• Korean Journal of Environmental Biology
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• v.33 no.1
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• pp.1-6
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• 2015

Increased harmful algal blooms by cyanobacteria are threatening public health and limiting human activities related with freshwater ecosystems. Phosphorus (P) has long been suggested as a critical nutrient for cyanobacterial bloom through field research in Canada during the 1970s, proposing a P-based freshwater management guideline. However, recently, nitrogen (N) has also been highlighted as an impacting nutrient on cyanobacterial harmful algal blooms (CyanoHABs). Due to the intensive and frequent observation of Microcystis, this kind of paradigm shift from P limitation to season-dependent N or P limitation has an important implication for a dual nutrient management strategy in eutrophic freshwaters. Through recent international researches, general strategies to control CyanoHABs in lakes and reservoirs are as follows: a dual nutrient (N & P) reduction, wastewater collection and treatment, pre-treatment of influent water in buffer zones, dredging of sediment, reduction of residence time, algal collection, and precipitation and flocculation of cyanobacteria. In addition, sustainable and integrative freshwater algae management should be carried out, based on the ecological aspect, because cyanobacteria are not the target organism to be eradicated, but an essential microbial member in the freshwater ecosystem.

• Ocean and Polar Research
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• v.33 no.2
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• pp.99-111
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• 2011

To estimate the effects of limitation nutrients for phytoplankton growth and its influences on short-term variations of a winter phytoplankton community structure, we investigated the abiotic and biotic factors of surface and bottom waters at 20 stations of inner and offshore areas from 6 to 7 February in Gwangyang Bay, Korea. Also, several algal bio-assay studies were conducted to identify any additional nutrient effects on phytoplankton assemblage using surface water for the assay. The dominant species in the bay was diatom Skeletonema costatum, which occupied more than 70% of total species in most stations (St.1-16) of the inner bay. According to a cluster and multidimensional scaling (MDS) analysis based on phytoplankton community data from each station, the bay was divided into three groups. The first group included stations from the south-western parts of Myodo lsland, which can be characterized as a semien-closed eutrophic area with high phytoplankton abundance. The second group included most stations from the north-eastern part of Myodo lsland, influenced indirectly by surface water currents from offshore of the bay. The standing phytoplankton crops were lower than those of the first group. The other cluster was restricted to samples collected from offshore of the bay. In the bay, silicon (Si) and phosphorus (P) were not a major limiting factor for phytoplankton production. However, since the DIN: DIP and DSi: DIN ratios clearly demonstrated that there were potential stoichiometric N limitations, nitrogen (N) was considered as a limiting factor. Based on the algal bio-assay, in vivo fluorescence values in N (+) added experiments were higher compared to control and P added experiments. Our results suggested that nitrogen may act as one of the most important factors in controlling primary production during winter in Gwangyang Bay.

• Proceedings of the Microbiological Society of Korea Conference
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• 2001.11a
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• pp.113-120
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• 2001

The production of microcystins from Microcystis aeruginosa was investigated in a P-limited continuous culture and a batch culture. The microcystin content of M aeruginosa was higher at a lower $\mu$, whereas the microcystin (MC)-producing rate was linearly proportional to $\mu$. The ratios of the MC-producing rate to the C-fixation rate were higher at a lower $\mu$. Consequently, increases in the microcystin content per dry weight along with the production of the more toxic form, MC-LR, were both observed under more P-limited conditions. The microcystin content of M. aeruginosa exhibited a high correlation with the total N content regardless of N-fixed or P-fixed culture. The microcystin concentration was investigated from spring to autumn in 1999 in the Daechung Reservoir, Korea. The dominant species in the algal blooming season was Microcystis. When the microcystin concentration exceeded about 100 ng $1^{-1}$ the ratio of particulate to dissolved total nitrogen (TN) or total phosphorus (TP) interestingly converged at a value of 0.6. The microcystin concentration was lower than 50 ng $1^{-1}$ at a particulate N:P ratio below 8, whereas the microcystin concentration varied quite substantially from 50 ng $1^{-1}$ to 250 ng $1^{-1}$ at a particulate N:P ratio> 8.

• Journal of Oral Medicine and Pain
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• v.44 no.3
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• pp.92-102
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• 2019

Purpose: To investigate the masticatory function of patients with different temporomandibular disorders (TMD) phenotypes, and to explore the risk factors for the masticatory function of TMD patients among multiple biopsychosocial variables using patient-reported outcomes (PROs). Methods: Clinical features and TMD diagnoses of 250 cases were investigated by reviewing medical records. Psychosocial factors were evaluated using four questionnaires representing pain severity and pain interference (Brief Pain Inventory), pain catastrophizing (Pain Catastrophizing Scale, PCS), psychological distress (Symptom Check List-90-Revised, SCL-90R) and kinesiophobia (Tampa Scale for Kinesiophobia for Temporomandibular Disorders, TSK-TMD). Masticatory function, as a dependent variable, was determined using the Jaw Functional Limitation Scale (JFLS). Kruskal-Wallis test and Spearman's rank correlation were used for analyses. Results: A total of 145 cases were included and classified into four subgroups including group 1: TMD with internal derangement without pain (n=14), group 2: TMD with muscle pain (n=32), group 3: TMD with joint pain (n=60) and group 4: TMD with muscle-joint combined pain (n=39). Pain severity (p=0.001) and interference (p=0.022) were the highest in group 2, but the mean global score of JFLS was the highest in group 3, followed by group 4, group 2, and group 1 (p=0.013). Pain severity, pain interference, the mean global score of PCS and the mean global score of TSK-TMD showed significant and moderate correlation with the mean global score of JFLS. All subdimensions and the global severity index of SCL-90R had significant, but weak correlations with all scores of JFLS. Conclusions: The results suggest that masticatory functional limitation depends on the TMD phenotypes. Among the various PROs, pain perception, pain catastrophizing and kinesiophobia seem to be more influential risk factors on jaw function than psychological distress, such as depression and anxiety.

• Journal of the Korean Institute of Telematics and Electronics
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• v.11 no.3
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• pp.22-26
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• 1974

The effects of anisotropic mechanical stress applied normal to the surface of p-n junctions have been investigated. As the stress increased, the breakdown voltage was decreased and the breakdown mode became softer. Within a certain limitation in the applied stress, the above phenomena werw reversibbe, though relaxation and hysteresis phenomena were observed. The time constant of relaxation depended upon the shape of the stressing tip, but for the given tip and device a unique time constant was obtained. The stress.dependence of breakdown voltage showed a good linearity up to about 3.0${\times}10^4$ kgw/$\textrm{cm}^2$, when the flat tip of radius 15$\mu$ was used, and the temperatere-dependence of breakdown voltage under the stress also showed a good linearity in the temperature range of 100 to $300^{\circ}K$.

• The Journal of Korean Obstetrics and Gynecology
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• v.26 no.1
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• pp.69-81
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• 2013

Purpose: The aim of this study is to determine associated factors affecting the utilization of Medical Care for an overactive bladder. Methods: Total 114 women with overactive bladder participated in this study. Patients were categorized in two groups, the patients with treatment experiences for an overactive bladder(n=40) and without treatment experiences for an overactive bladder(n=74). Patients completed Overactive Bladder Severity Score, Beck Depression Inventory, King's Health Questionnaire, and questionnaires on treatment experience. The results were analysed using SPSS for Windows 12.0k. All statistical tests were two-sided with a p-value < 0.05 being significant. Results: Patients with treatment experience had a greater score on KHQ, OABSS, BDI and had longer symptom duration period than those without treatment experience. And only Impact of Life(P=0.004), Role Limitation(P=0.004), Social Limitation(P=0.007), Emotion(P=0.035), Sleep/Energy(P=0.001), Incontinence Severity Measures(P=0.012) among KHQ domains were statistically significant difference between two groups. Conclusions: The most influencing factor associated with utilization of Medical Service in overactive bladder is the impact of the patient's quality of life.

• Korean Journal of Environmental Biology
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• v.31 no.4
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• pp.383-392
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• 2013

The objective of this study was to determine the effects of N:P ratio on primary productivity measured as chlorophyll-a (CHL) using the approach of In Situ Nutrient Enrichment Bioassays (NEBs) in Daechung Reservoir. The effects of NEBs on the N:P mass ratios were compared with the field data obtained from monthly-chemical monitoring during 2009~2012. The short-term NEBs showed that the response of primary productivity in the phosphorus spiked treatments (5, 15, 20 and 30 N:P ratios) were greater than the responses in the control (C) and nitrogen spiked treatment (N:P ratio=150, $T_{VI}$). The response in the nitrogen treatment (N:P ratio=150, $T_{VI}$) was less compared to control and all five treatments ($T_I{\sim}T_{VI}$). The outcomes of the NEBs suggest that phosphorus limited the phytoplankton growth and nitrogen addition inhibited the algal growth. In the analysis of nutrients and CHL from the ambient epilimnetic water in Daechung Reservoir, minimum N:P ratios resulted in maximum concentrations of CHL. Overall, our results suggest that the N:P ratio was the key factor in regulating the phytoplankton growth in NEB experiments.