• Title/Summary/Keyword: Mycorrhizal

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Effects of Mycorrhizal and Endophytic Fungi on Plant Community: a Microcosm Study

  • Park, Sang-Hyun;Eom, Ahn-Heum
    • Mycobiology
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    • v.35 no.4
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    • pp.186-190
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    • 2007
  • This study was conducted to investigate the effects of foliar endophytic fungi and arbuscular mycorrhizal fungi (AMF) on plant community structure in experimental microcosms containing an assemblage of five species of plants (Oenothera odorata, Plantago asiatica, Trifolium repens, Isodon japonicas and Aster yomena). Leaves of Sasa borealis, Potentilia fragarioides, and Viola mandshurica were collected in Chungbuk, Korea. Endophytic fungi were isolated from the surface sterilized leaves and identified to species level using molecular and morphological techniques. Four isolates of the endophytic fungi were inoculated to the leaves of host plants in the microcosms. Also, three species of AMF spores were extracted from pure cultures and the mixture of the three species inoculated to the roots of the plants. After four months of growth in a green house, effects of both symbiotic fungi on plant species diversity, community composition and productivity were examined. The plant species diversity showed significant differences with inoculation of the symbiotic fungi. Results indicate that AMF significantly affect plant productivity and plant community structure.

Effect of Dual Inoculation with Glomus mosseae and Rhizobium meliloti on Growth and Nitrogenase Activity of Medicago sativa L. (Glomus mosseae와 Rhizobium meliloti의 동시접종이 알팔파의 생장 및 질소고정활성에 미치는 영향)

  • 유익동;서현창;김원곤;박동진;조갑연;김창진
    • Microbiology and Biotechnology Letters
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    • v.21 no.2
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    • pp.95-100
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    • 1993
  • Effect of dual inoculation with vesicular-arbuscular(VA) mycorrhizal fungi, Glomus mosseae, and Rhizobium meliloti R455 on growth, nodulation, and nitrogenase activity of alfalfa (Medicago sativa L. cv. Vernal) were examined in pot experiment. After 63 days growth, shoot length, number of leaf, and leaf size of alfalfa were increased as as result of dual inoculation with Glomus mosseae and Rhizobium meliloti. Total dry weight of alfalfa plant was increased 1.4 times compared to single inoculation with Rhizobium meliloti. Nodule number and mean fresh weight of nodule per plant were also increased due to the mycorrhizal infection.

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The Effect of Benomyl Treatments on Ginsenosides and Arbuscular Mycorrhizal Symbiosis in Roots of Panax ginseng

  • Eo, Ju-Kyeong;Eom, Ahn-Heum
    • Journal of Ginseng Research
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    • v.33 no.4
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    • pp.256-259
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    • 2009
  • The effects of benomyl treatment on ginsenoside and arbuscular mycorrhizal (AM) symbiosis in the roots of Panax ginseng that were collected from two sites in Korea were investigated. The ginseng roots that were treated with benomyl showed different species compositions of AM fungi colonizing the ginseng roots, compared to untreated roots. In the analysis of ginsenoside, Rc was significantly higher in benomyl untreated roots than in benomyl treated roots. The results suggest that AM fungal species composition and ginsenosides in ginseng root could be influenced by the benomyl treatment.

Biodiversity and Distribution of Arbuscular Mycorrhizal Fungi in Korea

  • Eo, Ju-Kyeong;Park, Sang-Hee;Lee, Eun-Hwa;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.42 no.4
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    • pp.255-261
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    • 2014
  • In this study, we summarized previous studies on diversity and distribution of arbuscular mycorrhizal fungi (AMF) for last 30 years in Korea. According to a review of the literature concerning AMF in Korea, 14 genera and 89 species have been recorded. Host plants for AMF are very diverse and include crop species and woody plants in natural forests. Based on the achievements of the last 30 years of study on AMF, we anticipate that relatively more intensive studies of the functional and genetic diversity of AMF will be conducted.

Identification of Arbuscular Mycorrhizal Fungi from Botrychium ternatum Native in Korea

  • Lee, Jun-Ki;Eom, Ahn-Heum;Lee, Sang-Sun
    • Mycobiology
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    • v.32 no.4
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    • pp.179-185
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    • 2004
  • Arbuscular mycorrhizal fungi were observed in Botrychium ternatum native in Korea. The partial small subunit(SSU) of ribosomal DNA gene from the fern roots was amplified with primers AM1/NS31. Nucleotides sequence analysis of the clones revealed that two fragments were close to Glomus proliferum and G. sinuosum. The other three DNA fragments were close to those of G. proliferum with the relatively low similarities($92{\sim}95%$) and speculated to be originated from three different species of Glomus(GLA006, GLA016, and GLA032). Five different nucleotide sequences close to three AM fungal species were found in the roots of B. ternatum native in Korea.

Effect of Organic Farming on Spore Diversity of Arbuscular Mycorrhizal Fungi and Glomalin in Soil

  • Lee, Ji-Eun;Eom, Ahn-Heum
    • Mycobiology
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    • v.37 no.4
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    • pp.272-276
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    • 2009
  • In this study, eight soil samples were collected from organic and conventional farms in a central area of South Korea. Spore communities of arbuscular mycorrhizal fungi (AMF) and glomalin, a glycoprotein produced by AMF, were analyzed. Spores of Glomus clarum, G. etunicatum, G. mosseae, G. sp., Acaulospora longula, A. spinosa, Gigaspora margarita, and Paraglomus occultum were identified at the study sites, based on morphological and molecular characteristics. While Acaulospora longula was the most dominant species in soils at organic farms, Paraglomus occultum was the most dominant species in soils at conventional farms. Species diversity and species number in AMF communities found in soils from organic farms were significantly higher than in soils from conventional farms. Glomalin was also extracted from soil samples collected at organic and conventional farms and was analyzed using both Bradford and enzyme-linked immunosorbent assays. The glomalin content in soils from organic farms was significantly higher than in soils from conventional farms. These results indicate that agricultural practices significantly affect AMF abundance and community structure.

Effects of Arbuscular Mycorrhizal Fungal Inoculation on the Growth of Red Pepper and Soil Glomalin Content

  • Lee, Ji-Eun;Lee, Eun-Hwa;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.49 no.4
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    • pp.517-524
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    • 2021
  • Red pepper seedlings were inoculated either alone or with a mixture of all five species of arbuscular mycorrhizal fungi (AMF). After 10 weeks of growth in the greenhouse, the seedlings were transplanted into fields and cultivated without chemical fertilizers and pesticides for 10 weeks. The results showed that plant growth was significantly increased under both greenhouse and field conditions, suggesting that AMF inoculation has a positive effect on the growth of Capsicum annuum and improves the physical properties of the soil by increasing the concentration of glomalin. The application of AMF can positively contribute to sustainable agriculture by reducing the use of chemical fertilizers while increasing crop growth.

Mycorrhizal Formation of Korean Truffle Species, Tuber koreanum with Oak Trees

  • Ju-Hui Gwon;Ahn-Heum Eom
    • The Korean Journal of Mycology
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    • v.51 no.2
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    • pp.121-125
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    • 2023
  • In this study, we examined the ectomycorrhiza (ECM) formed by a species of the genus Tuber, T. koreanum, with two indigenous Korean oak species, Quercus acutissima and Q. dentata. T. koreanum showed a high colonization rate for both oak species, with a higher mean colonization rate observed for Q. acutissima. The mycorrhizal characteristics of T. koreanum were described, revealing distinct morphological and anatomical differences between the ECM formed with the two host plants. This study is significant because it is the first to elucidate ECM formations of T. koreanum in indigenous oak trees. However, further studies are required to differentiate the ECM associations formed by T. koreanum from those of other Tuber species.

Artificial Production of Ectomycorrhizae Truffle spp. (균근성 트러플의 생산 가능성)

  • Chang, H.Y
    • Journal of Practical Agriculture & Fisheries Research
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    • v.8 no.1
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    • pp.63-70
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    • 2006
  • Truffles are cultivated by growing seedlings of compatible tree species in pasteurized substrate amended with truffle spores, maintaining the inoculated seedlings in the greenhouse until the mycorrhizal relationship is established and certified, planting the infected seedlings onto appropriate and properly prepared sites, and managing the resulting truffieres (truffle orchards) to maintain the truffle infections and encourage truffle fruiting. Questions remain to be addressed at each step of the process in developing best management practices for truffle cultivation.