• Journal of agriculture & life science
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• v.46 no.6
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• pp.157-163
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• 2012

Pleurotus eryngii. one of the most popular edible mushrooms, has been well known for its biological activities such as antioxidation, antitumor and immune modulation. Spent coffee ground(SCG) that is a waste product from the coffee industry has been continuously investigated for its reutilization. In this study, SCG was added to the fungal cultuvation medium and analyzed for its effect on the growth and physiological activity of P. eryngii mycelia. It was clearly demonstrated that SCG could accelarate mycelia growth. 1% SCG culture was very notable by showing 2.5-fold higher dry cell weight comapred to the control culture, which suggested SCG as an excellent activator for the growth of P. eryngii mycelia. By the addition of SCG, polyphenol content was increased by two fold but there was no change in polysaccharide content. In the analysis of DPPH scavenging activity, SCG was determined as a valuable source in order to significantly increase the antioxidative activity of the mycelium.

• Journal of Mushroom
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• v.8 no.3
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• pp.102-108
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• 2010

In this study, growth rates and enzyme activities of mushroom mycelium were investigated in each cereals. Cultivation on hulled barley resulted in slightly faster mycelial growth as compared to other substrates. Enzyme activities were measured in different periods. In result, ${\alpha}$-amylase activity was higher in Pleurotus ostreatus and Phellinus linteus cultured cereals, whereas ${\beta}$-amylase activity was higher in Pleurotus ostreatus and Fomitella fraxinea cultured. Protease level did not affect kinds of cereal except Phellinus linteus. The color values lightness decrease by Basidiomycetes cultivation and redness and yellowness increased.

• The Korean Journal of Mycology
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• v.17 no.1
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• pp.39-47
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• 1989

Among 100 species in 54 families of plants tested, leaf extracts from Allium cepa, Allium sativum, Malussieboldii, Reynoutria japonia and Rheum coreanum were inhibitory on mycelium growth of phytophthora spp.. Especially, Allium sativum and Malus sieboldii were strongly inhibitory. Allium sativum, Malussieboldii and Rhem coreanum were strongly inhibitory on zoosporangium germination of P. capsici. Malus sieboldii only were strongly inhibitory on zoosporangium germination of P. nicotiana and P. infestans.And Malus sieboldii were strongly effected on disease control of these pathogens. These results indicate the possibility of finding antagonistic plants in the nature for the control of certain pathogens in soil.

• The Plant Pathology Journal
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• v.22 no.2
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• pp.107-114
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• 2006

The white cottony stem rot pathogen Sclerotinia scierotiorum was subjected to 70 different isolates of actinomycetes indigenous to Jordan as biological control agents. Forty of them demonstrated chitinase activity on crab shell chitin agay (CCA) media and they were segregated into three groups: 14 highly active, 12 moderately active, and 14 with low activity, with average clearing zones of (4.7-8.3), (3.7-4.3), and (2.3-3.3) mm surrounding colonies on CCA, respectively. Further, these isolates were able to inhibit radial mycelium growth of the pathogen and were categorized into three antagonistic groups: 13 strong, 13 moderate, and 14 weak antagonists, with antibiosis inhibition Bones of (32.0-45.7), (22.7-31.3), and (3.7-22.3) mm, respectively. High levels of chitinase activity of the isolates Ma3 (8.3 mm), Jul (7.7 mm), and Sa8 (7.7 mm) with their antagonistic activity against mycelium growth of 45.7, 44.3, and 40.7 mm were observed, respectively. These isolates exhibited fungicidal activity against sclevotia of S. sclerotiorum. On the other hand, isolates Na5, Aj3, and Aj2 that produced no chitinase showed fungistatic effect only.

• Mycobiology
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• v.43 no.3
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• pp.327-332
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• 2015

Phenylalanine ammonia-lyase (PAL) gene is known to be expressed in plants, and is involved in the differentiation, growth and synthesis of secondary metabolites. However, its expression in fungi remains to be explored. To understand its expression in mushroom fungi, the PAL gene of the edible mushroom Flammulina velutipes (Fvpal) was cloned and characterized. The cloned Fvpal consists of 2,175 bp, coding for a polypeptide containing 724 amino acids and having 11 introns. The translated amino acid sequence of Fvpal shares a high identity (66%) with that of ectomycorrhizal fungus Tricholoma matsutake. Distinctively, the Fvpal expression in the mycelium was higher in minimal medium supplemented with L-tyrosine than with other aromatic amino acids. During cultivation of the mushroom on sawdust medium, Fvpal expression in the fruit body correspondingly increased as the mushroom grew. In the fruiting body, Fvpal was expressed more in the stipe than in the pileus. These results suggest that F. velutipes PAL activity differs in the different organs of the mushroom. Overall, this is first report to show that the PAL gene expression is associated with mushroom growth in fungi.

• Journal of Mushroom
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• v.20 no.3
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• pp.93-101
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• 2022

Cordyceps militaris mycelium extracts containing high amounts of cordycepin were evaluated in vitro for their anti-inflammatory and tumor cell growth-inhibitory activities. All extracts dose dependently inhibited the increased production of inflammatory mediators including reactive oxygen species (ROS), nitric oxide (NO), and 𝛽-hexosaminidase in lipopolysaccharide (LPS)-stimulated inflammatory cells. All extracts were evaluated for anti-proliferative activity against normal RBL-2H3 cells and diverse types of cancer cell lines, including HCT, MC5-7, U-87MG, AGS, and A549 cells. The extract showed the strongest growth inhibition (IC₅₀ = 28.13 ㎍/mL) relative to vehicle-treated control cells against fibrosarcoma (MC5-7). We have demonstrated anti-inflammatory activity of C. militaris via inhibition of NO, ROS production, and 𝛽-hexosaminidase release in activated cells. C. militaris mycelium extract was also evaluated mechanistically and found to exert six types of anti-cancer activity, confirming its pharmacological potential. Our study suggests C. militaris use as a potential source of anti-inflammatory and anti-cancer agents. C. militaris may also be considered a functional food.

• Mycobiology
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• v.32 no.2
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• pp.95-97
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• 2004

In July 2002, a destructive stem rot of tawny daylily(Hemerocallis fulva) was occurred sporadically in exhibition farm of Gyeongsangnam-do Agricultural Research and Extension Services located in Hamyang-gun, Korea. The fungus also caused collar and crown rot, and systemic wilt or blight of whole plant. White mycelium spread over stems and petioles of infected plants and sclerotia were formed on the old lesions and near the soil surface. The optimum temperature for mycelial growth and scierotial formations was $30^{\circ}C$ on PDA. The mycelial width ranged $4.2{\sim}10.4{\mu}m$ and the color was white, usually many narrow mycelial strand grew in the aerial mycelium and formed clamp connection. The shape of sclerotia was spherical and $1.0{\sim}3.2$ mm in diameter. The fungus was isolated repeatedly from the infected tissues and confirmed its pathogenicity to Hemerocallis fulva and identified as Sclerotium rolfsii. This is the first report on the stem rot of H. fulva caused by S. rolfsii in Korea.

• KSBB Journal
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• v.14 no.3
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• pp.365-370
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• 1999

To optimize the culture conditions for Beauveria bassiana 726, the effects of culture medium, pH, and temperature on mycelium and spore production were investigated. The optimum temperature and pH for the cultivation of B. bassiana 726 were 28 $^{\circ}C$ and 5.0, respectively. The optimized medium was composed of 1.0~2.0% total sugar from molasses, 0.5% corn steep liquor and 0.05% KH$_2$PO$_4$. In the cultivation of B. bassiana 726 with the optimum medium, the specific growth rate and substrate utilization were well-fitted with the proposed kinetic model in the shake flask and stirred tank reactor. When the fed-batch cultivation using carbon suorce, nitrogen source, and mineral salt as a feeding medium was compared with batch cultivation in stirred tank reactor, mycelium (12.7 g/L) and spore production (5.4$\times$$10^8/mL$) were enhanced up to 110% and 85%, respectively.

• Journal of Microbiology and Biotechnology
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• v.12 no.5
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• pp.760-765
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• 2002

Mucor miehei KCTC 6011, which grew successfully in ginseng-steaming effluents and produced a large amount of chitosan efficiently, was selected from various fungi. Approximately 120 mg of chitosan per g-dry mycelium was maximally produced in 84 h at $25^{\circ}C$ when grown in the ginseng-steaming effluent (pH 8.0) supplemented with 0.5% yeast extract and 0.002% CuSO$_4$. Chitosan produced by Mucor miehei KCTC 6011 was identified by the IR-spectra to have deacety lated approximately 56%. Viscosity and molecular weight of the chitosan were 80 cps and $1.07\times10^3$ kDa, respectively. The chitosan at 1.5 mg/ml inhibited 73.9% of the mycelium growth of Rhizoctonia solani in 60 h.

• The Plant Pathology Journal
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• v.20 no.2
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• pp.103-105
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• 2004

A destructive stem rot of strawberry (Fragaria x ananassa cv. Akihime) sporadically occurred in farmers' fields in Daegok-myon, Jinju city, Gyeongnam province in Korea. The infected plants showed stem and crown rot, with occasional blighting of the whole plant. White mycelia appeared on stems of infected clones and sclerotia formed on the old lesions near soil surface. The fungus formed white colony on PDA and showed maximum mycelial growth and sclerotial formation at $30^{\circ}C$. The fungus usually have many narrow hyphal strands, 2.6-10.0 $\mu\textrm{m}$ in width, in the aerial mycelium. Typical clamp connections were formed on the mycelium. Sclerotia were spherical and 1.0-2.4 mm in size. The fungus was repeatedly isolated from infected tissues and identified as Sclerotium rolfsii. Its patho-genicity was confirmed when inoculated onto straw-berry. This is the first report on the stem rot of strawberry caused by S. rolfsii in Korea.