• 한국균학회지
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• 제49권4호
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• pp.525-530
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• 2021

The cultivation in sawdust media, mushroom productivity, and fruiting body characteristics of Lentinula edodes strains NIFoS 2778 and NIFoS 3363 were compared according to the inoculation conditions. The cultivation period was 5% shorter when liquid spawn was used. Fruiting bodies were induced after 113 days of incubation on media inoculated with liquid spawn, and the cultivation period was 119 days on media inoculated with solid spawn. Mushroom productivity of NIFoS 2778 was the highest (661.4 g) when 36 mL of liquid spawn was used. For NIFoS 3363, mushroom production was higher under liquid inoculation conditions when the same amount of liquid and solid spawns were used. The mushroom characteristics of the two strains were not significantly different, except for gill width and stipe diameter.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2018년도 춘계학술대회 및 임시총회
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• pp.29-29
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• 2018

Sparassis latifolia is called "Cauliflower Mushroom" and is known as an edible mushroom that has high content of ${\beta}$-glucan. Recently, artificial cultivation of S. latifolia has been done by bottle, plastic bag and wood cultivation in Korea. However it is not widely used because there are low incubation ratio and yield. For the high efficiency of production, we aim to find the superior strains and media for better mycelial and fruit body growth. First, we analyzed the genetic relationship among 31 strains and divided five groups with three kinds of URP primers. And then ten strains were selected from five groups based on the experiment of mycelial growth. The suitability of media for mycelial growth was different according to media type. The suitable solid and liquid media for mycelial growth of S. latifolia isolates were PDA and M2, respectively. In addition, with regard to C/N ratio, the mycelial growth increased even until C/N 160. Second, we investigated the production of fruitbody of the strains by plastic bag cultivation. The substrate was mixed with larch sawdust, corn flour, and wheat flour (8:1:1, v/v). Moisture content of substrate was controlled by about 60% with 10% molasses solution. Out of 31 strains, 19 strains formed primordia. The eight strains produced more than 140g/1kg in fresh weight. Third, molasses culture media was selected for the mycelial growth. And molasses suitable sugar content and input aeration were around 8Brix% and 0.3~0.6vvm, respectively. The longer the incubation period is, the more dried weight of mycelia increased, but medium volume decreased. Therefore, the best incubation period was 9 to 11 days depending on strains. In the future, research project entitled development of culture system and new variety for stable production of S. latifolia will be considered as a new item.

• 임산에너지
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• 24권2호
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• pp.31-36
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• 2005

꽃송이버섯은 $1,3-{\beta}-D-glucan$의 함량이 다른 버섯에 비하여 훨씬 높은 식용버섯이다. 이 버섯은 항암 및 면역증강 효과가 큰 식용버섯으로 알려지면서 최근에는 일본과 한국에서 재배하고 있다. 하지만, 쉽게 재배할 수 있는 방법이 알려지지 않고 일부 기업이 특허권의 범주 내에서 재배를 하고 있는 형편이다. 본 연구는 이러한 문제를 해결하고자 간단한 공정으로 꽃송이 버섯을 재배 할 수 있는 방법을 개발한 것이다. 낙엽송, 소나무, 잣나무의 톱밥을 이용하여 보릿가루와 설탕을 80:20:3의 중량 비율로 섞은 후 수분함량을 65% 내외로 맞춘 병배지에서 꽃송이버섯을 생산할 수 있었다. 톱밥 종류 및 균주에 따라서 자실체 생산성은 다르게 나타났는데 650g의 배지에서 최고 177g(수율 27%)를 수확할 수 있었다. 따라서 실용적인 꽃송이버섯 재배방법으로 이 배지를 적극 추천한다. 단, 생산성 향상을 위해서는 보릿가루의 적정 농도와 톱밥종류에 맞는 균주의 선발이 지속적으로 필요하다.

• Mycobiology
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• 제42권1호
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• pp.41-45
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• 2014

The effectiveness of three kinds of enzymes (chitinase, ${\beta}$-glucuronidase, and lysing enzyme complex), employed as elicitors to enhance the ${\beta}$-glucan content in the sawdust-based cultivation of cauliflower mushroom (Sparassis latifolia), was examined. The elicitors were applied to the cauliflower mushroom after primordium formation, by spraying the enzyme solutions at three different levels on the sawdust-based medium. Mycelial growth was fully accomplished by the treatments, but the metabolic process during the growth of fruiting bodies was affected. The application of a lysing enzyme resulted in an increase in the ${\beta}$-glucan concentration by up to 31% compared to that of the control. However, the treatment resulted in a decrease in mushroom yield, which necessitated the need to evaluate its economic efficiency. Although we still need to develop a more efficient way for using elicitors to enhance functional metabolites in mushroom cultivation, the results indicate that the elicitation technique can be applied in the cultivation of medicinal/edible mushrooms.

• 한국버섯학회지
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• 제16권4호
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• pp.342-346
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• 2018

기후 변화에 따른 표고 재배사내 온도와 습도의 변화 추이를 알아보기 위하여 5년 동안 봄, 여름, 가을 기간에 걸쳐 국내 49곳의 표고버섯 재배사에서 온도와 습도를 측정 분석하였다. 톱밥배지 재배사의 5년간 평균 온도와 습도는 $24.7^{\circ}C$와 60.5%이었다. 원목 재배사의 5년간 평균 온도는 $24.4^{\circ}C$, 평균 습도는 60.0%로 나타났다. 여름철 기간 중 온도 평균은 원목재배사의 경우는 2016년 $29.8^{\circ}C$, 2017년 $29.1^{\circ}C$, 2018년 $33.3^{\circ}C$이었으며 톱밥배지 재배사의 경우는 2016년 $26.8^{\circ}C$, 2017년 $20.4^{\circ}C$, 2018년 $24.2^{\circ}C$이었다. 조사 기간 중 $30^{\circ}C$가 넘는 고온으로 측정된 재배사는 봄철에 1곳, 여름철에 5곳이었으며 원목 재배사가 5곳을 차지하였다. 원목재배에서 온도가 $20^{\circ}C$를 넘지 않는 재배사는 4곳으로 모두 가을에 조사된 온도였다. 본 연구 조사는 기후변화에 대비해 표고 재배사의 경우 원목 재배사 관리에 우선적으로 대비해야 함을 시사하였다.

• 한국균학회지
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• 제25권4호통권83호
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• pp.268-275
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• 1997

High prices of raw materials used as media for the mushroom cultivation increased the cost of commercial production of oyster mushroom (Pleurotus ostreatus). In this study, garlic peels (Allium sativum f. pekinese) as an agricultural by-product were investigated to replace the saw dust for the bottle cultivation of oyster mushroom. Mycelial growth of oyster mushroom were examined by the extracts made from the sawdust, rice bran and garlic peels. The mycelial growth was very poor in the agar media containing the extracts of sawdust or garlic peels, but was good when those of the rice bran were added. In the polypropylene bottle experiment, the sawdust medium which ammended with minerals vitamin was essential for the production of the mushroom fruitbodies. The rice bran was considered to stimulate the mycelial growth, but not the development of basidiocarps. The garlic peel was not a factor to stimulate the production of mushroom fruitbodies, but a raw material ammended with the rice bran produced much amounts of mushroom. In this work, garlic peels ($10{\sim}70%$ v/v) added to the mixture of sawdust and rice bran (4 : 1, v/v) was considered to help the productions of mushroom fruitbodies. Based on the result, the replacement of expensive saw dust with inexpensive garlic peels was a good example to reduce production cost of the bottle cultivation of mushroom.

• The Plant Pathology Journal
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• 제35권6호
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• pp.674-683
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• 2019

Some species of the Trichoderma genus are reported as the major problem in oak wood mushroom production in Korea. In spite of economic loss by the fungi, scientific information on airborne Trichoderma species is not much available. To generate information for disease management development we analyzed airborne Trichoderma. A total of 1,063 fungal isolates were purely obtained from indoor air sampling of cultivation houses used for oak wood mushroom using sawdust media. Among the obtained isolates, 248 isolates were identified as Trichoderma fungi including T. harzianum, T. atroviride, T. citrinoviride, and T. pseudokoningii, by morphological and molecular analysis. T. harzianum was dominant among the four identified species. All the four Trichoderma species grew fast on solid nutrient media tested (potato dextrose agar [PDA], malt extract agar [MEA], Czapek's Dox + yeast extract agar [CYA] and cornmeal dextrose agar). Compact mycelia growth and mass spore production were better on PDA and CYA. In addition, T. harzianum and T. citrinoviride formed greenish and yellowish mycelium and spores on PDA and CYA. Greenish and yellowish pigment was saturated into PDA only by T. pseudokoningii. These four Trichoderma species could produce extracellular enzymes of sawdust substrate degradation such as β-glucosidase, avicelase, CM-cellulase, amylase, pectinase, xylanase, and protease. Their mycelia inhibited the growth of oak wood mushroom mycelia of two tested cultivars on dual culture assay. Among of eleven antifungal agents tested, benomyl was the best to inhibit the growth of the four Trichoderma species. Our results demonstrate that the airborne Trichoderma fungi need to be properly managed in the cultivation houses for safe mushroom production.

• Journal of the Korean Wood Science and Technology
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• 제21권1호
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• pp.7-14
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• 1993

To increase the production of mushrooms and the economic efficiency of mushroom cultivation, the pinchips(PC) and sawdusts(SD) of three Korean oak species were tested as cultivating media for Lentinus edodes with varing the composition of the media. The results are as follows. 1. Organic acids(tannic acid and citric acid), which were added in the PC medium to enrich the quality of mushrooms, did not increase the growths of the mycelia and the quality. 2. The mushrooms were able to be harvrested from six months after beginning the cultivation. No significant differences were found among the qualities of the mushrooms produced by various treatments. 3. The production rate of mushroom was relatively high with, 28.4~29.3% (700~730gr./2,500gr pack). The net profit estimated was about 1,450 won per pack.

• 한국버섯학회지
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• 제9권4호
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• pp.145-154
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• 2011

표고 수집균주의 특성을 파악하고 재배적 특성과 갈변과의 상관관계를 알아봄으로써 표고 톱밥재배를 안정적으로 실시하고 재배기간을 단축시킬 수 있는 방법을 모색하고자 하였다. 한천배지에서의 균사생장은 균주와 배지에 따라 다르게 나타났으며, MEA와 PDA에서는 큰 차이가 없었다. 균사생장 최적온도는 $20{\sim}25^{\circ}C$ 이었다. 200lux의 광조건에서 갈변패턴에 따라 균주를 분류할 수 있었다. 갈변기간은 한천배지에서는 30-40일, 톱밥봉지에서는 70~100일이 소요되었다. 자실체 특성을 관찰한 결과 300g 이상 수량을 보이는 균주가 12균주였으며, 자실체 수량 및 재배특성을 고려할 때 ASI 3046이 가장 우수한 것으로 판단되었다. 균사생장과 갈변 간의 유의성은 인정되지 않았으며, PDA배지와 톱밥 균사생장과는 유의성이 인정되었으며, 한천배지에서 갈변되는 기간과 톱밥배지에서 갈변되는 기간과의 관계는 고도 유의 상관을 나타내었다. 따라서 균이 갈변되는 성질은 영양분의 차이보다는 균의 고유한 성질이라고 생각되며 갈변이 빠른 균주를 선발하기 위해서는 한천배지를 이용하여 균을 선발할 수 있을 것으로 생각된다. 앞으로 톱밥재배에 필수적인 갈변에 대한 이해를 실제 재배에 접목시켜 톱밥재배 기간을 단축시키거나 생산성을 비약적으로 증대시키는 등 톱밥재배를 활성화시킨다면 품질이 높은 생표고를 낮은 가격으로 생산해 낼 수 있으리라 생각된다.

• 한국균학회지
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• 제15권2호
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• pp.92-98
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• 1987

느타리버섯 볏짚배지(培地)에 발생하는 유해균류(有害菌類)를 조사하고 그중 발생(發生) 및 피해(被害)가 큰 균류(菌類)의 배양적(培養的) 성질에 관한 실험을 실시한 결과를 요약하면 다음과 같다. 1. 느타리버섯 볏짚배지(培地)에서 12종류(種類)의 진균류(眞菌類)가 분리(分離)되었는데 그 중 Trichoderma, Aspergillus, Rhizopus 등은 발생빈도(發生頻度)가 높고 발생양(發生量)도 많았다. 2. 느타리버섯 볏짚배지(培地)에서는 7종(種)의 Trichoderma가 동정되었는데 발생빈도(發生頻度)는 T. pseudokoningii, T. aureoviride, T. viride, T. harzianum, T. koningii의 順이었다. 3. 유해균류(有害菌類)의 발생(發生)은 일반 버섯재배사(栽培舍)에서 재배회수(栽培回數)가 증가(增加)할수록 많았고 추기재배(秋期栽培)보다는 춘기재배(春期栽培)에서 심하였다. 4. 볏집추출배지(抽出培地) 및 느타리버섯 추출배지(抽出培地)에서 Trichoderma, Aspergillus, Rhzopus 등 공시한 균들의 균사생장과 분생포자의 형성이 양호한 결과를 보이므로서 볏짚배지(培地) 및 균상(菌床)의 버섯과 그 파열물들이 균류(菌類)의 발생(發生)에 알맞는 조건을 부여하였다. 5. Trichoderma spp.는 톱밥추출배지(抽出培地) 및 미강추출배지(米糠抽出培地)에서 생장(生長)이 극히 양호하여 균상(菌床)에 접종(接種)한 종균(種菌)은 균류(菌類)의 배지(培地)가 되었다.