• Title/Summary/Keyword: Mushroom bed

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A Study on Establishment of Similar Expousre Groups(SEGs) for Chemical and Biological Risk Factors in Farm Work (농작업시 발생하는 화학적 및 생물학적 위험요인에 대한 유사노출작업군 설정 연구)

  • Lee, Minji;Sin, Sojung;Kim, Hyocher;Heo, Jinyoung;Ahn, Minji;Kim, Kyungran;Kim, Kyungsu
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • 제30권3호
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    • pp.292-298
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    • 2020
  • Objectives: The aim of this research is to establish Similar Exposure Groups (SEGs) for chemical and biological risk factors that occur in farm work involving 24 tasks among 15 crops. Methods: To categorize SEGs, work type, work environment, and similar tasks for each crop were considered. After confirming the chemical risk factors (pesticides, inorganic dust-total dust and PM10, ammonia, and hydrogen sulfide) and biological factors (organic dust-total dust and PM10, and endotoxins) that occur in the crops and tasks, similar crops and tasks were selected as SEGs. Results: Among chemical risk factors, pesticides was selected for the SEGs, which was categorized by open field, greenhouse, fruit, and specialty crops. For inorganic dust, open field (plowing harrowing, seedling, planting, harvest, and sorting and packing) and specialty crops (plowing harrowing, seedling, planting, and harvest) were selected as SEGs. For ammonia and hydrogen sulfide, livestock (preparation of farm, management of nursery bed, feeding, shipment and manure treatment) were selected as SEGs. For biological risk factors such as organic dust (total dust, PM10) and endotoxins, open field (manure application), greenhouse (plowing harrowing, planting, manure application, and harvest), fruit (manure application), specialty crops (manure application, making furrows, mixing mushroom media, harvest, and sorting and packing), and livestock (preparation of farm, maintaining poultry litter, feeding, shipment and manure treatment) were selected as SEGs. Conclusions: To establish similar exposure groups in agriculture, it is important that the characteristics of each hazard factor are categorized by identifying risk factors occurring by tasks.

Study Case on the Log Cultivation of Phellinus baumii for It's High Quality and Large Quantity (고품질 다수확 원목 상황버섯 재배 경영사례 조사)

  • Suh, Gyu-Sun;Chang, Hyun-You;Kim, Soon-Geun
    • Journal of Practical Agriculture & Fisheries Research
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    • 제10권1호
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    • pp.153-167
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    • 2008
  • High temperature and natural sun light are considered as the core conditions for high quality and large quantity of Phellinus baumii production. However still now on there has been a mistake of excessively cutting off the natural light by spreading the closing nets on the mushroom cultivating house. For an example there are many houses where the closing nets under the roofs be extended to cover the sides of the houses, which way prevents the mushrooms in the houses from receiving sufficient natural sun light and getting the temperature sufficiently to grow so that the quantity and quality of the produced mushrooms are lowered even though the mushrooms can grow in those conditions. In order to avoid this mistake, the closing nets must be placed on the roofs of the houses only without dropping them to cover the sides. Further more when the closing nets are placed triply at the beginning stage of Phellinus baumii's growth in the house, the nets restrain the internal temperature of the house going up and intercept the natural bright light flowing into the house so that the growing tardiness occur to the Phellinus baumii. Therefore the roof only must have been covered by the closing net for 65% cutting off the light until May, and then covered by double folded the net for June, triple folded the net for July and August, double folded the net for September, and the single net for October. When the ventilation in the house has been maintained until the house tightly balloon out through controling lifting force of internal air, the Phellinus baumii can grow well while the bed logs themselves aren't dried out. Marketing is also very much important as well as increasing quality and quantity of Phellinus baumii production.

The Analysis of Management and the Method of Cultivation of Lentinus edodes I. for Full-Development of Mycelium in Bed Logs (표고재배(栽培)의 관리분석(管理分析)과 종균활착(種菌活着)을 위한 골목관리(管理)에 관(關)한 연구(硏究))

  • Joo, Myoung Chil
    • Journal of Korean Society of Forest Science
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    • 제85권4호
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    • pp.596-604
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    • 1996
  • This study was carried out to offer the successive method of cultivation and increase the productivity of mushroom yield with good quality through the elevation of rate of spawn development for Lentinus edodes. Studied about the analysis of current management of actural cultivation, a base of these, researched and presented for the upward method of productivity through an experiment of the high rate of spawn development and cultivation, putting first cultural environment. The results obtained were as follows ; 1. As the result of the analysis of current management in actural cultivation, many cultivators had a tendency to neglect managements of cultivation. These were reason for the deficiency of labour, funds and the lack of knowledge of cultivation, etc. 2. Water contents in bed logs according to the date of inoculation was shown as the decreasing order of 28.63%(3/12), 25.20%(3/25) and 23.19%(4/10). For the purpose of the maintenance of the water contents, the full-development of mycelium in bed logs and the dispersion of labour, the date of inoculation should be started in the early March. 3. The difference of the rate of spawn development among species was not shown, 100%(Mori 465). 98.98%(Mori 3046) on the spawn in high temperature and 98.97%(Mori 290) on the spawn in low temperature. The relative rate of spawn development was 97.70%(Mori 465), 82.45%(Mori 3046) on the spawn in high temperature and 88.87%(Mori 290) on the spawn in low temperature, it showed the difference. The spawn should be selected carefully in the future, as the spawn of cultivater's preference showed the difference for the development of mycelium. 4. The rate of spawn development following the date of inoculation was 100.0%(3/12), 98.98%(3/25) and 96.79%(4/10) on the spawn in high temperature and 99.09%(3/12), 98.97%(3/25) and 97.89% (4/10) in low temperature, it showed little difference. And the relative rate of spawn development was 97.70%(3/12), 82.45%(3/25) and 81.42%(4/10) on the spawn in high temperature and 93.27%(3/12), 89.67%(3/25) and 88.87%(4/10) that in low temperature, As the result of the relative rate, the time of inoculation of spawn should begin in the early March. 5. The height of stock logs on temporary placing should be less than 60cm at most on the surface, because of the low rate of water contents.

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Biological control of mushroom flies using the predatory mite Hypoaspis aculeifer in a shiitake cultivation (원목 표고에서 아큐레이퍼응애를 이용한 버섯파리류의 생물학적 방제)

  • Kim, Hyeong Hwan;Kim, Dong Hwan;Yang, Chang Yeol;Kwon, Sun Jung;Jeon, Sung Wook;Song, Jin Sun;Cho, Myoung Rae;Lee, Chan Jung;Cheong, Jong Chun
    • Journal of Mushroom
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    • 제11권4호
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    • pp.230-239
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    • 2013
  • The major species of fungus gnats which caused the severe damage in shiitake farm were identified as a Bradysia difformis, B. alpicola, and Camtomyia cortocalis on oak log beds cultivation. The B. difformis occurred early in the middle of March while B. alpicola and C. cortocalis appeared since the beginning of May. The occurrence rate for adults of B. difformis showed highly at the end of July (11.9~1,774.2 in dong-myeon and 0.4~2,583.3 in pungse-myeon) in 2012 and mid-June (10.7~4,650 in dong-myeon and 36.8~4740 in pungse-myeon) in 2013. The counting numbers on the traps for B. alpicola reached highest peak in the middle of June (2.1~63.2 in dong-myeon and 1.0~21.7 in pungse-myeon) and the end of May (0.8~163.7 in dong-myeon and 0.5~280.5 in pungse-myeon) in 2012 and 2013, respectively. The number of C. cortocalis showed high record in the middle of May in 2012 (0.6~4.7) and in the middle of June (2.1~17.3) in 2013 in dong-myeon whereas showed the peaks in the middle of May (0.6~4.7) in 2012 and in the late of May (1.3~17.6) in 2013 in pungse-myeon. The fruiting bodies of shiitake mushroom by fungus gnats were severely damaged from mid-June to late-July and the damage rate were 0.625.5% (2012) and 0.7~30.5% (2013) in dong-myeon and 1.5%~21.6% (2012) and 1.9~36.8%(2012) in pungse-myeon. To investigate the control effect for fungus gnats by Hypoaspis aculeifer, H. aculeifer (30 mixutre of nymph and adult per $m^2$) were treated to oak log beds shiitake cultivation for six times (May 2 and 28, June 25, July 10 and 25 and August 28). The occurrence rate of adults and damage rate of fruiting bodies of 3 major species reduced 79.3% (adult numbers) and 74.8% (fruiting bodies) in dong-myeon and 64.1% (adult numbers) and 65.5% (fruiting bodies) in pungse-myeon, respectively, compared to non-treatment. Accordingly, H. aculeifer effectively controlled the fungus gnats on shiitake mushroom and can be used as good control agent.

Control of Diatrype stigma occurred on the bed-log of Shiitake by resistant Shiitake strains (표고골목 해균인 주홍꼬리버섯을 방제하기 위한 저항성 표고균주 선발)

  • Lee, Bong-Hun;Bak, Won-Chull;Ka, Kang-Hyeon;Yoon, Kab-Hee;Park, Hyun;Cha, Byeong-Jin
    • Journal of Mushroom
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    • 제3권3호
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    • pp.109-114
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    • 2005
  • Attempts were made to control Diatrype stigma occurred on the bed-log of shiitake by resistant shiitake strains. In selection test of resistant shiitake strains, 67 out of 77 strains tested were proved to be resistant to D. stigma. Among them, 13 strains including KFRI 5 were effective to inhibit the access of D. stigma, and 7 strains including KFRI 180 remarkably invaded the territory of D. stigma. Among 31 shiitake strains made by hybridization of resistant strains for D. stigma, 8 strains including KFRI 537 inhibited the access of D. stigma, and 4 strains including KFRI 545 invaded the territory of D. stigma. The effects of temperatures and inoculation orders to the resistance were confirmed in PDA plates and test tubes filled with sawdust of Quercus acutissima. Four kinds of temperature treatments as follows were tested: (1) continuous incubation at $14^{\circ}C$, (2) continuous incubation at $25^{\circ}C$, (3) changing of incubation temperature from $14^{\circ}C$ to $25^{\circ}C$ as soon as mycelia of both shiitake and D. stigma meet together, (4) changing of incubation temperature from $25^{\circ}C$ to $14^{\circ}C$ as soon as mycelia of both shiitake and D. stigma meet together. Three kinds of inoculation procedure were tested: (1) inoculation of shiitake 3 days ahead of D. stigma inoculation, (2) inoculation of D. stigma 3 days ahead of shiitake inoculation, (3) simultaneous inoculation of both fungi. In PDA plate test, the strain KFRI 137 showed outstanding ability to inhibit mycelial growth of D. stigma and the strain KFRI 180 invaded into the territory of D. stigma in most of treatments. Hybrid strains, KFRI 545, 546, and 547 were more resistant than their parent strains, KFRI 488 and 405. In test tube examinations, all the strains of shiitake showed high resistance at the treatment of change in temperature from $14^{\circ}C$ to $25^{\circ}C$ when mycelia of both shiitake and D. stigma meet together. On the other hand, resistance of all the strains growing at $25^{\circ}C$ decreased when the temperature was changed into $14^{\circ}C$ after mycelia of both fungi. In these cases, the resistance reached to 7~20% of the highest resistance. The strain KFRI 259 invaded the territory of D. stigma, contrary to PDA plate test. Among the strains, KFRI 393 strain was the most resistant under the continuous incubation at $25^{\circ}C$.

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Synergistic effect of phosphate solubilization by Burkholderia strains isolated from button mushroom bed (양송이배지로부터 분리한 Burkholderia균의 인산가용화 공조효과)

  • Park, Ji-Hoon;Han, Chang-Hoon;Yoon, Min-Ho
    • Journal of Mushroom
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    • 제15권4호
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    • pp.183-189
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    • 2017
  • This study investigated the synergistic effect of single inoculation and co-inoculation of phosphate-solubilizing bacteria (PSB) Burkholderia metallica JH-7 and Burkholderia contaminans JH-15. Phosphate-solubilizing abilities of these strains were assessed by measuring phosphorus content in culture media that were singly inoculated or co-inoculated with these strains for 7 days. B. metallica JH-7 was found to release the highest content of soluble phosphorus ($140.80{\mu}g\;mL^{-1}$ ) into the medium, followed by single inoculation of B. contaminans JH-15 ($135.95{\mu}g\;mL^{-1}$ ) and co-inoculation of two strains ($134.84{\mu}g\;mL^{-1}$ ). The highest pH reduction, organic acid production, and glucose consumption were observed in the medium inoculated with B. metallica JH-7 alone compared with that in the medium co-inoculated with both the strains. Results of a plant growth promotion bioassay showed 17.4% and 7.48% higher leaf and root growth, respectively, in romaine lettuce inoculated with B. metallica JH-7 alone than in romaine lettuce inoculated with a control strain. However, no significant difference was observed between single inoculation and co-inoculation of these strains with respect to phosphorus release and plant growth. Although the results of the present study did not show the synergistic effect of phosphate solubilization by the PSB strains examined, these results indicate that treatment with PSB exerts a beneficial effect on crop growth.

Studies on the Physico-Chemical Characteristics of Different Casing Materials Affecting Mycelial Growth and Yield of Cultivated Mushroom, Agaricus bisporus (Lange) Sing. (양송이의 균사생장(菌絲生長) 및 자실체(子寶體) 수량(收量)에 미치는 복토재료(覆土材料)의 이화학적(理化學的) 성질(性質)에 관(關)한 연구(硏究))

  • Kim, Dong-Soo
    • The Korean Journal of Mycology
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    • 제3권1호
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    • pp.1-19
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    • 1975
  • Since the importance of casing in fruit body formation of Agaricus bisporus has been emphasized, physico-chemical characteristics of casing materials were discussed by many workers and a mixture of peat and mineral soil as proper casing material has been adopted in many of mushroom growing countries. Because of limited resources of peat in Korea, it is necessary to find practical performance and substitutional materials for casing. The effect of casing on mycelial growth and mushroom yield of A. bisporus varied with materials, its combination and practices etc. The experiments to be discussed in this paper are concerned with pH and Ca of casing material which influence A bisporus, and changes of physico-chemical characteristics with mixing ratio of casing materials and its effect on A. bisporus. The optimum range of moisture content of each material, management of watering and application of physico-chemical characteristics casing materials was also investigated and re-use of weathered spent compost for casing material was described. 1. The effect of calcium on mycelial growth of A. bisporus at various pH in Halbschalentest showed different results with calcium sources. Best results were obtained around neutrality and fresh weight of fruit bodies grown in the range of pH 7 to 8 was highest among the tested levels. 2. Available moisture, pore space, organic matter, cation exchangeable capacity and exchangeable cation was increased by an increase of mixing ratio of peat in casing materials, while an adverse effect was obtained by addition of sand. 3. Mycelial growth on clay loam was more rapid at a lower bulk density of 0.75g/cc and at 20% moisture content on a dry weight basis at the same bulk density. 4. Mixing ratio of casing materials, 60 to 80 per cent by volume of peat mixed with 20 to 40 per cent of clay loam produced the highest yield of fresh fruit bodies and sand the lowest. However, per cent of open cap was highest in peat and lowest in sand. 5. Days required for fruit body initiation was shortened in mixtures of peat and clay loam by one to three days compared with other materials and the formation of flushes was clear. 6. The effect of some physico-chemical characteristics of casing materials on the fresh weight of fruit bodies were estimated by a multiple regression equation; Y=-923.86+$8.18X_1+8.04X_2+7.90X_3+0.12X_4+2.03X_5-0.82X_6-0.54X_7$ where $X_1,X_2,X_3,X_4,X_5,X_6,X_7$ are sand, silt, clay, available moistuer, porosity, organic matter and exchangeable cation respectively. The productivity of certain casing material could be predicted from this equation. 7. Fresh weight of fruit bodies was positively correlated with porosity exchangeable cation, organic matter, available moisture, silt and clay of materials; while sand was negatively correlated. On the contrary, sand was the unique factor reducing per cent of open cap. 8. Distribution of three phases of high productive casing material was concentrated in the range of 10 to 30 per cent solids, 15 to 30 per cent liquids, and 50 to 60 per cent in air volume. 9. Fresh weight of fruit bodies from peat was not affected with heavy watering but in clay loam and sandy loam severe crop losses occurred. Fresh weight of individual fruit was increased and open caps were decreased with heavy watering but light watering resulted in adverse effects: its effect was especially great in peat. 10. Optimum range of moisture content by weight on a dry basis was different with each casing material. To maintain optimum moisture content concerned with yield of fruit bodies and open cap, sandy loam and peat mixtures required daily watering of 0.6, 0.6 to 1. 2 and 1.2 to 2.4 liters per $3.3m^2$ of bed area, respectively. 11. Maximum yield of fruit body was recorded in the range of pF 2. 0 to 2. 5 of casing materials if organic matter content was below 4.2 per cent and in pF 1. 3 to 1.8 if above 7.1%. 12. pF curve of a certain casing material could be draws from moisture content at various pF values by multiple regression equations provided texture, organic matter and calcium of the casing material are given. Optimum moisture range of the casing materials also could be estimated by the equation. 13. It was possible to improve the phyico-chemical characteristics of clay loam and sandy loam by addition of weathered spent compost although the effect was less than in the case of peat. Fresh weight of fruit bodies wsa increased by addition of weathered spent compost but its effect was not as remarkable as peat. Accordingly, further studies will be required.

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TECHNICAL STUDY ON THE CONTROLLING MECHANIQUES OF THE ENVIRONMENTAL FACTORS IN THE MUSHROOM GROWING HOUSE IN CHONNAM PROVINCE (전남지방(全南地方)에 있어서의 양송이 재배(栽培)에 최적(最適)한 환경조건(環境條件) 조절법분석(調節法分析)에 관(關)한 연구(硏究))

  • Lee, Eun Chol
    • Journal of Korean Society of Forest Science
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    • 제9권1호
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    • pp.1-44
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    • 1969
  • The important results which have been obtained in the investigation can be recapitulated as follows. 1. As demostrated by the experimental results and analyses concerning their effects in the on-ground type mushroom house, the constructions in relation to the side wall and ceiling of the experimental houses showed a sufficient heat insulation on effect to protect insides of the houses from outside climatic conditions. 2. As the effect on the solar type experimental mushroom house which was constructed in a half basement has been shown by the experimental results and analyses, it has been proved to be effective for making use of solar heat. However there were found two problems to be improved for putting solar houses to practical use in the farm mushroom growing: (1) the construction of the roof and ceiling should be the same as for the on-ground type house, and (2) the solar heat generating system should be reconstructed properly. A trial solar heat generating system is shown in Fig. 40. 3. Among several ventilation systems which have been studied in the experiments, the underground earthen pipe and ceiling ventilation, and vertical side wall and ceiling ventilation systems have been proved to be most effective for natural ventilation. 4. The experimental results have shown that ventilation systems such as the vertical side wall and underground ventilation systems are suitable to put to practical use as natural ventilation systems for farm mushroom houses. These ventilation systems can remarkably improve the temperature of fresh air which is introduced into the house by heat transfers within the ventilation passages, so as to approach to the desired temperature of the house without any cooling or heating operation. For example, if it is assuming that x is the outside temperature and y is the amount of temperature adjustment made by the influence of the ventilation system, the relationships that exist between x and y can be expressed by the following regression lines. Underground iron pipe ventilation system ${\cdots}{\cdots}$ y=0.9x-12.8 Underground earthen pipe ventilation system ${\cdots}{\cdots}$y=0.96x-15.11 Vertical side wall ventilation system${\cdots}{\cdots}$ y=0.94x-17.57 5. The experimental results have shown that the relationships existing between the admitted and expelled air and the $Co_2$ concentration can be described with experimental regression lines or an exponent equation as follows: 1) If it is assumed that x is an air speed cm/sec. and y is an expelled air speed in cm/sec. in a natural ventilation system, since the y is a function of the x, the relationships that exist between x and y can be expressed by the regression lines shown below: 2) If it is assumed that x is an admitted volume of air in $m^3/hr$ and y is an expelled volume of air in $m^3/hr$ in a natural ventilation system, since the y is a function of the x, the relationships that exist between x and y can be expressed by the regression lines shown below. 3) If it is assumed that the expelled air speed in cm/sec and replacement air speed in cm/sec. at the bed surface in a natural ventilation system are shown as x and y, respectively, since the y is a function of the x, the relationships that exist between x and y can be expressed by the following regression line: G.E. (100%)- C.V. (50%) ventilation system${\cdots}$ y=0.54X+0.84 4) If it is assumed that the replacement air speed in cm/sec. at the bed surface is shown as x, and $CO_2$ concentration which is expressed by multiplying 1000 times the actual value of $CO_2$ % is shown as y, in a natural ventilation system, since the y is a function of the x the relationships that exist between x and y can be expressed by the following regression line: G.E. (100%)- C.V. (50%) ventilation system${\cdots}{\cdots}$ y=114.53-6.42x 5) If it is assumed that the expelled volume of air is shown as x and the $CO_2$ concentration which is expressed by multiplying 1000 times the actual of $CO_2$ % is shown as y in a natural ventilation system, since the y is a function of of the x, the relationships that exist between x and y can be expressed by the following exponent equation: G.E. (100%)-C.V. (50%) ventilation system${\cdots}{\cdots}$ $$y=127.18{\times}1.0093^{-X}$$ 6. The experimental results have shown that the ratios of the crass sectional area of the G.E. and C.V. vent to the total cubic capacity of the house, required for providing an adequate amount of air in a natural ventilation system, can be estimated as follows: G.E. (admitting vent of the underground ventilation)${\cdots}{\cdots}$ 0.30-0.5% (controllable) C.V. (expelling vent of the ceiling ventilation)${\cdots}{\cdots}$ 0.8-1.0% (controllable) 7. Among several heating devices which were studied in the experiments, the hot-water boilor which was modified to be fitted both as hot-water toiler and as a pressureless steam-water was found most suitable for farm mushroom growing.

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A Study of the Application of Folk Medicine in Patients with Diabetes Mellitus (당뇨병 환자의 민간요법 시행에 대한 실태조사)

  • Um, Dong-Chun;Lee, Young-Shin
    • Journal of East-West Nursing Research
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    • 제1권1호
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    • pp.72-81
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    • 1997
  • Korean society is a mix of western and traditional cultures. Even though the patients try to recover through both aspects, hospital only utilizes a western approachs. When they have suffered from a chronic disease, especially diabeties meliitus(DM), application of folk medicine is more varied but the nurses are unaware of the practices. This study was done to describe the current use of the folk medicine with which the patients with DM have had an experience and to identify the relationships between the westernized medical care and folk medicine application. The 244 patients with non-insulin dependent diabetes millitus who participated were interviewed at a DM education clinic in a 1500-bed hospital. The results of the study are as follows: 1. 54.1%(N=133) of the participated patients in this study have had a folk medicine. 2. 44 kinds of folk medicine were used for the treatment of DM. Among them 14 items were used by more than two people and the rest were used by one person. Among the used items, 70.4% consisted of various types of plants, 11.4% was animal material and 18. 2% was the mixed group. As a single item, Commelina Communis(Dalgaebi) was the most frequently used(62.8%), followed by the root of Rosa rugosa(Haedangwha) 14.3%, and Youngi mushroom 13.6%. 3. In the analysis of the relationships between the general characters of the patients with regular fol1ow-up(F-U), self blood sugar test(BST) and folk medicine usage: 1) The higher the educational back ground patient had, the lower the folk medicine usage was ($X^2$=14.265, P=.003). 2) The more complex their treatment was($X^2$=24. 016. P=.000). and the longer they had suffered from DM($X^2$=75.739, P=.000), the fewer they visited regular F-U. On the other hand, they did self-BST well($X^2$=7.722, P=.021 : $X^2$=14. 775, P=.002) and had more folk medicine($X^2$=33.382, P=.000 ; $X^2$=43.410, P=.000). 3) If they had suffered many symptoms, they had fewer regular F-U ($X^2$=13.192. P=.001). On the other hand, they had more folk medicine($X^2$=6.070, P=.048). 4) The group of family history($X^2$=6.801. P=.009) and the group of DM education experience($X^2$=15.678, P=.000) carried out self BST well. DM education group used more folk medicine($X^2$=8.680, P=.003). In conclusion, DM education should be grouped according to the treatment type and suffering period. Then the management of DM would be effective and the vague application of folk medicine would be decreased.

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On the Mushroom Cultivation of Oak (Quercus) Chip and Used Culture Medium of Lentinus into Feedstuff (참나무류(類) 칩을 이용(利用)한 표고버섯재배(栽培)와 폐잔사(廢殘渣) 사료화(飼料化))

  • Min, Du Sik
    • Journal of Korean Society of Forest Science
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    • 제80권4호
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    • pp.436-444
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    • 1991
  • To increase both the production of mushrooms and the usages of the residues (used media) for roughage, the pinchips(PC) and sawdusts(SD) of three Korean oak species were tested as cultivating media for Lentinus edodes with varing the composition of the media. The results are as follows. 1. Organic acids(tannic acid and citric acid), which were added in the PC medium to enrich the quality of mushrooms, did not increase the growths of the mycelia and the quality. 2. The mushrooms were able to be harvested from six months after beginning the cultivation. No significant differences were found among the qualities of the mushrooms produced by various treatments. 3. The contents of crude proteins(1.82-4.55%) in the used media were higher than those in woods (c.a. 0.7%), and the total digestible nutrients (44.0-46.0%) in the used medium were not much different from those in rice straws (c.a.48.0%). 4. The contents of some essential amino acids (methionine in the used medium of the tannic acid added PC or S.D. and isoleucine, phenylalanine and lysine in the used medium of the untreated PC or SD) were higher than those in the control (raw media). The contents of a few other essential amino acids (threonine, valine and arginine) were lower in the used media. Most nonessential amino acids in the used media, particularly in the used bed-log, appeared to decrease than those in the control.

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