• Journal of Microbiology and Biotechnology
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• 제19권9호
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• pp.851-857
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• 2009

We developed and characterized 36 polymorphic microsatellite markers for the oyster mushroom (Pleurotus ostreatus). In total, 169 alleles were identified with an average of 4.7 alleles per locus. Values for observed ($H_o$) and expected ($H_E$) heterozygosities ranged from 0.027 to 0.946 and from 0.027 to 0.810, respectively. Nineteen loci deviated from Hardy-Weinberg equilibrium. Significant (P<0.05) excess heterozygosity was observed at nine loci. Linkage disequilibrium (LD) was significant (P<0.05) between pairs of locus alleles. Cluster analysis revealed that five species of genus Pleurotus made a distinct group, and the individual cultivars were grouped into major five groups from G-1 to G-5. The diverse cultivars of P. ostreatus were discriminated and the other four species revealed a different section in the UPGMA tree. These microsatellite markers proved to be very useful tools for genetic studies, including assessment of the diversity and population structure of P. ostreatus.

• 대한전자공학회논문지TC
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• 제45권8호
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• pp.8-14
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• 2008

본 논문에서는 via가 있는 버섯구조를 이용한 ENG ZOR 안테나와 DGS 구조를 이용한 via-free ENG ZOR 안테나의 접지면 크기의 변화에 따른 입력저항, 대역폭, 방사효율의 영향에 대하여 이론적 연구를 수행하였다. Via-free ZOR 안테나가 via가 있는 버섯구조 ZOR 안테나보다 접지면 크기에 영향을 더 받는 것으로 확인되었다. DGS가 적절하게 동작하기 위해서는 최소 크기의 접지면이 요구되어지므로 via-free 안테나의 방사특성은 일정 크기 이상의 접지면이 확보되어야 좋아질 수 있다. Via가 있는 안테나는 기판의 높이가 높아질수록 대역폭과 방사효율이 좋아지고, via-fee 안테나의 경우 높이가 높아질수록 대역폭과 방사효율이 떨어지는 것으로 관찰되었다. 또한, via-free ZOR 안테나의 경우 via가 있는 버섯구조 ZOR 안테나와 비교하여 대역폭이 좁고 방사효율은 떨어지지만 소형화에 유리함을 알 수 있었다.

• 한국버섯학회지
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• 제13권3호
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• pp.207-211
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• 2015

Kenaf(Hibiscus cannabinus L.)의 버섯 배지원으로서의 활용 가능성을 판단하고자 물리성과 영양원 함유량 등을 분석한 결과 배지의 형태를 유지하고 영양원의 공급기지 역할을 하는 주재료로서 활용 가능성이 높았으며 탄소, 질소 함량과 C/N율, 일반성분, 미량원소 그리고 아미노산 함량 등도 일정 수준을 유지하고 있어서 배지 영양원으로서 역할도 기대할 수 있었다. 실제로 Kenaf는 다공성 세포구조 특성으로 높은 함수능력을 가지고 있었는데 bast 부위의 경우 수분 흡수율이 578%로 수분 조절제와 산소 공급자 역할을 충실히 할 것으로 판단되었다. 총 탄소 함량은 91.4%로 포플러톱밥이나 밀기울, 미강에 비해 아주 높았다. 총 질소는 1.76%로 배지 주재료로는 높은 특성을 유지하였으며 C/N율은 51.9 였다. 가용성무질소물은 46.6%로 미강 정도의 수준을 유지하였다. 탄소원 구성을 보면 cellulose 함량은 포플러에 비해 상대적으로 높았고 lignin 함량은 낮았는데, 비교적 원활하게 사용할 수 있는 탄소원인 hemicellulose+pectin 혼합물이 포플러에 비해 3.7% 많았다. 무기이온 함량도 케나프가 포플러톱밥에 비해 전체적으로 높았는데 Fe은 4.2배, P은 3.2배, K는 2.2배 많았고 Ca는 16 mg/kg 더 많았다. 아미노산 함량은 전체적으로 포플러톱밥보다 많았으며 미강보다는 함량이 낮았다.

• 한국통신학회논문지
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• 제39B권1호
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• pp.44-52
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• 2014

본 연구에서는 마이크로스립 패치 안테나의 접지 면으로 버섯 형태 EBG(Electromagnetic Band Gap)를 사용할 경우의 근거리 장(near field) 원거리 장(far field) 방사 지향성(radiation directivity)의 영향을 연구하였다. 버섯 모양 EBG의 분산 곡선 특성을 이용하여 주어진 버섯모양 EBG의 금지대역(band gap) 2.36-2.85[GHz]를 계산하고, 금지대역 영역에 속하는 주파수로 동작하는 2-층의 버섯 형태 EBG 마이크로스트립 안테나(2.45GHz)를 설계하였다. EBG를 접지 면으로 사용한 마이크로스트립 안테나의 성능향상을 확인하기위하여 PEC(Perfect Elecric Conductor)를 접지 면으로 사용한 동일한 안테나 경우와 성능차이를 비교하였다. EBG를 사용할 경우 유전체와 도체의 경계면에서 발생하는 표면파(Surface Wave)를 효과적으로 억제하여 지향성이 약 2.74dB 정도 향상된 것을 알 수 있었다.

• Biomolecules & Therapeutics
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• 제28권3호
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• pp.230-239
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• 2020

Previous studies have shown disrupted synaptic plasticity and neural activity in depression. Such alteration is strongly associated with disrupted synaptic structures. Chronic stress has been known to induce changes in dendritic structure in the basolateral amygdala (BLA) and medial prefrontal cortex (mPFC), but antidepressant effect on structure of these brain areas has been unclear. Here, the effects of imipramine on dendritic spine density and morphology in BLA and mPFC subregions of stressed mice were examined. Chronic restraint stress caused depressive-like behaviors such as enhanced social avoidance and despair level coincident with differential changes in dendritic spine structure. Chronic stress enhanced dendritic spine density in the lateral nucleus of BLA with no significant change in the basal nucleus of BLA, and altered the proportion of stubby or mushroom spines in both subregions. Conversely, in the apical and basal mPFC, chronic stress caused a significant reduction in spine density. The proportion of stubby or mushroom spines in these subregions overall reduced while the proportion of thin spines increased after repeated stress. Interestingly, most of these structural alterations by chronic stress were reversed by imipramine. In addition, structural changes caused by stress and blocking the changes by imipramine were corelated well with altered activation and expression of synaptic plasticity-promoting molecules such as phospho-CREB, phospho-CAMKII, and PSD-95. Collectively, our data suggest that imipramine modulates stress-induced changes in synaptic structure and synaptic plasticity-promoting molecules in a coordinated manner although structural and molecular alterations induced by stress are distinct in the BLA and mPFC.

• 한국버섯학회지
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• 제15권1호
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• pp.54-56
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• 2017

Wolfiporia cocos is a well-known traditional medicine in China, Japan, Korea, and other Asian countries owing to its numerous therapeutic properties. With the aim to determine the morphology and genetic characteristics of W. cocosten strains of W. cocos were cultivated in vitro, and subsequently, rapid amplification of polymorphic DNA was performed. To the best of our knowledge, this is the first study to examine the morphology of fruit bodies of W. cocos in Korea. W. cocos were cultured on PDA agar at different temperatures (12, 16, 20, 24, and $28^{\circ}C$) under 12-hour light (600 Lux) / 12-hour dark photoperiod condition for 1 month. Appearance of fruit body was the highest at $28^{\circ}C$ condition in all the strains investigated. Honeycomb-like structure on sclerotia was observed in Andong 01, Andong 02, Andong 03, KFRI 1104, KFRI 1105, KFRI 1106, KFRI 1107, KFRI 1108, and ASI 13007 strains of. The KFRI 1103 strain formed cosmos petal-like structure on sclerotia. The average size of basidiospores was recorded as $7.55{\mu}m$ in height and $3.35{\mu}$ in width.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 1993년도 Proceedings of International Conference for Agricultural Machinery and Process Engineering
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• pp.1243-1253
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• 1993

Visual features of a mushroom(Lentinus Edodes L) are critical in sorting and grading as most agricultural products are. Because of its complex and various visual features, grading and sorting of mushrooms have been done manually by the human expert. Though actions involved in human grading looks simple, a decision making undereath the simple action comes form the results of the complex neural processing of the visual image. And processing details involved in the visual recognition of the human brain has not been fully investigated yet. Recently, however, an artificial neural network has drawn a great attention because of its functional capability as a partial substitute of the human brain. Since most agricultural products are not uniquely defined in its physical properties and do not have a well defined job structure, a research of the neuro-net based human like information processing toward the agricultural product and processing are widely open and promising. In this pape , neuro-net based grading and sorting system was developed for a mushroom . A computer vision system was utilized for extracting and quantifying the qualitative visual features of sampled mushrooms. The extracted visual features and their corresponding grades were used as input/output pairs for training the neural network and the trained results of the network were presented . The computer vision system used is composed of the IBM PC compatible 386DX, ITEX PFG frame grabber, B/W CCD camera , VGA color graphic monitor , and image output RGB monitor.

• 대한전자공학회논문지TC
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• 제45권4호
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• pp.43-52
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• 2008

패치 안테나 주위에 mushroom 형태를 가지는 EBG 구조를 길이 방향, 폭 방향과 모든 방향으로 집적한 경우 구조 파라미터에 따른 패치 안테나의 방사 특성을 연구하였다. EBG 구조가 길이 방향을 따라 집적된 경우가 가장 방사 특성이 좋음을 볼 수 있었다. EBG 패치 edge에서 패치 안테나의 피드까지의 거리가 같은 경우가 패치 안테나의 중심까지의 거리가 같은 경우와 비교하여 E-평면 방사패턴이 대칭적이고 전방방사 크기가 커지고 후방방사 크기가 작아짐을 볼 수 있었다. 사용하는 EBG 구조의 주기 수가 4주기 이상이 되게 되면 방사특성 변화는 거의 발생하지 않음을 볼 수 있었다.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 춘계학술대회 및 임시총회
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• pp.45-45
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• 2014

Individualities of precious health mushroom called Zhenghonggu@ from respective protections scattered among all main mountains of Fujian China were collected and recognized locally, then compared with Russula griseocarnosa. Their internal transcribed spacer (ITS) region (ITS1, ITS2 and 5.8S rDNA) of the nuclear rDNA were amplified, AMOVA analyzed, nested clade analyzed and then compared with the ITS sequences of relative Russula species from other regions of China to confirm the taxonomic status of Zhenghonggu$^@$ and its population structure. Total 23 haplotypes from different protections of Fujian can be clustered into three clades similar to the three lineages of Dahongjun$^@$ from southeastern China reported by Li et al. The geographic distribution characteristic of these three phylogeny clades may be closely coupled with the vegetation regionalization and/or the differences of coenosium construction of Fagaceae that is the host of Russula griseocarnosa. The correlation of taxonomy, phylogeny and geographical distribution of Russula are discussed.

• 한국버섯학회지
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• 제2권2호
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• pp.45-48
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• 2004

The effect of $CO_2$ concentration (500, 3,000, $6,000{\mu}{\ell}/{\ell}$) on the mycelial growth and fruit body primordium formation of Ganoderma lucidum on nutrient agar medium was examined. Optimum $CO_2$ concentration for vegetative growth was above $3,000{\mu}{\ell}/{\ell}$. Fruit body initiation was accelerated at higher than $3,000{\mu}{\ell}/{\ell}$ $CO_2$ exposure but the maximum number and size of primordia, and primordium color were not influenced by $CO_2$ concentrations. Whereas each atypical fruiting structure forming stock culture showed different fruiting time under each concentration of $CO_2$ exposure.