• Nutrition Research and Practice
• /
• 제12권2호
• /
• pp.129-134
• /
• 2018

BACKGROUND/OBJECTIVES: Although several recent studies have reported the anti-cancer effects of extracts or components of Citrus unshiu peel, which has been used for various purposes in traditional medicine, the molecular mechanisms for their effects remain unclear. In the present study, the anti-cancer activity of a water-soluble extract of C. unshiu peel (WECU) in MDA-MB-231 human breast carcinoma cells at the level of apoptosis induction was investigated. MATERIALS/METHODS: Cytotoxicity was evaluated using the MTT assay. Apoptosis was detected using DAPI staining and flow cytometry analyses. Mitochondrial membrane potential, reactive oxygen species (ROS) assay, caspase activity and Western blotting were used to confirm the basis of apoptosis. RESULTS: The results indicated that WECU-induced apoptosis was related to the activation of caspase-8, and -9, representative initiator caspases of extrinsic and intrinsic apoptosis pathways, respectively, and caspase-3 accompanied by proteolytic degradation of poly(ADP-ribose) polymerase and down-regulation of the inhibitors of apoptosis protein family members. WECU also increased the pro-apoptotic BAX to anti-apoptotic BCL-2 ratio, loss of mitochondrial membrane potential and cytochrome c release from mitochondria to cytoplasm. Furthermore, WECU provoked the generation of ROS, but the reduction of cell viability and induction of apoptosis by WECU were prevented when ROS production was blocked by antioxidant N-acetyl cysteine. CONCLUSIONS: These results suggest that WECU suppressed proliferation of MDA-MB-231 cells by activating extrinsic and intrinsic apoptosis pathways in a ROS-dependent manner.

• PNF and Movement
• /
• 제21권1호
• /
• pp.87-94
• /
• 2023

Purpose: The present study aims to investigate the effects of a diagonal exercise pattern on selective activation of the upper extremity muscles using both normalization and isolation methods. Methods: In total, 17 asymptomatic subjects participated in this study. During the two diagonal patterns of exercise (diagonals 1 and 2), muscular activities of the upper trapezius (UT), lower trapezius (LT), serratus anterior (SA), anterior deltoid (AD), and infraspinatus (IS) were measured. The collected data were analyzed in two ways, according to the dominance of muscle activities (%Isolation) and according to normalized activities (%MVIC). Results: There were significant differences in LT, SA, AD, and IS between %MVIC and %Isolation (p<0.05), and the diagonal 1 pattern of exercise showed significantly more LT activities compared with the diagonal 2 pattern (p<0.05). Further, except for LT, there were no significant differences in muscle activities between the diagonal 1 and 2 exercises. Conclusion: The present study suggests that a diagonal pattern of exercise is advantageous for strengthening shoulder muscles, but caution is needed when applying to patients requiring selective strengthening. Regarding both the concentric and eccentric phases of exercise, there was no significant difference in muscular activation, except in LT, between the two diagonal patterns of exercises.

• Toxicological Research
• /
• 제34권1호
• /
• pp.13-21
• /
• 2018

Anthocyanins are naturally occurring water-soluble polyphenolic pigments in plants that have been shown to protect against cardiovascular diseases, and certain cancers, as well as other chronic human disorders. However, the anti-obesity effects of anthocyanins are not fully understood. In this study, we investigated the effects of anthocyanins isolated from the fruit of Vitis coignetiae Pulliat on the adipogenesis of 3T3-L1 preadipocytes. Our data indicated that anthocyanins attenuated the terminal differentiation of 3T3-L1 preadipocytes, as confirmed by a decrease in the number of lipid droplets, lipid content, and triglyceride production. During this process, anthocyanins effectively enhanced the activation of the AMP-activated protein kinase (AMPK); however, this phenomenon was inhibited by the co-treatment of compound C, an inhibitor of AMPK. Anthocyanins also inhibited the expression of adipogenic transcription factors, including peroxisome proliferator-activated receptor-${\gamma}$, CCAAT/enhancer-binding protein a and b, and sterol regulatory element-binding protein-1c. In addition, anthocyanins were found to potently inhibit the expression of adipocyte-specific genes, including adipocyte fatty acid-binding protein, leptin, and fatty acid synthase. These results indicate that anthocyanins have potent anti-obesity effects due to the inhibition of adipocyte differentiation and adipogenesis, and thus may have applications as a potential source for an anti-obesity functional food agent.

• 대한의용생체공학회:의공학회지
• /
• 제28권5호
• /
• pp.636-646
• /
• 2007

Adequate postural control depends on the spatial and temporal integration of vestibular, visual, and somatosensory information. Especially, the musculoskeletal function is essential to maintain the postural control. The experimental studies was performed on the muscular activities in the lower extremities during maintaining and moving exercises on an unstable platform with Magneto Rheological(MR) dampers. The unstable platform of the developed system was controlled by electric currents to the MR dampers. A subject executed the maintaining and moving exercises which are presented through the display monitor. The electromyographies of the eight muscles in lower extremities were recorded and analyzed in the time and the frequency domain: the muscles of interest were rectus femoris(RF), biceps femoris(BF), tensor fasciae latae(TFL), vastus lateralis(VL), vastus medialis(VM), gastrocnemius(Ga), tibialis anterior(TA), Soleus(So). The experimental results showed that the muscular activities differed in the four moving exercises and the nine maintaining exercises. For the anterior-posterior pattern, the TA showed highest activities; for the left-right pattern, the TFL; for the 45, $-45^{\circ}$ pattern, the TFL and TA. Also, the rate of the increase in the muscular activities were affected by the condition of the unstable platform with MR dampers for the maintaining and moving exercises. The experimental results suggest that the choice of different maintaining and moving exercises could selectively train different muscles in various intensity. Futhermore, the findings suggested that the training using this system can improve the ability of postural control.

• 한국전문물리치료학회지
• /
• 제23권1호
• /
• pp.80-86
• /
• 2016

Background: Prone hip extension (PHE) can be performed to measure the lumbopelvic motor patterns and motions. Imbalances in lumbopelvic muscle activity and muscle weakness can result in instability including pain in lumbopelvic region. The posterior oblique sling (POS) muscles contribute to dynamic lumbopelvic stability. In addition, POS are anatomically aligned with the trapezius muscle group according to shoulder positions. Objects: This study compared the electromyography (EMG) activity of POS and pelvic compensations during PHE with and without pre-activation of lower trapezius muscle (lowT). Methods: Sixteen healthy males were recruited. PHE was performed in randomized order: PHE with and without lowT pre-activation. Surface EMG signals were recorded for biceps femoris (BF), gluteus maximus (GM) (ipsilateral), lumbar multifidus (MF) (bilateral), and the lowT (contralateral). An electromagnetic tracking motion analysis was used to measure the angle of pelvic rotation and anterior tilting. Results: The ipsilateral GM and bilateral MF EMG amplitudes were greater during PHE with lowT pre-activation compared to PHE without lowT pre-activation (p<.05). The BF amplitude during PHE without lowT pre-activation was significantly greater than that during PHE with lowT pre-activation (p<.05). The angles of pelvic rotation and anterior tilting during PHE with lowT pre-activation were significantly smaller compared to PHE without lowT pre-activation (p<.05). Conclusion: PHE with lowT pre-activation, which is aligned with the POS, showed more increased MF and GM muscular activity with smaller lumbopelvic compensations in rotation and anterior tilting compared to PHE without lowT pre-activation.

• 대한물리치료과학회지
• /
• 제19권4호
• /
• pp.53-59
• /
• 2012

Background : The purpose of the study was: to investigate muscle activation of upper arm according mouse shape. Methods : Twenty person(mean age : 23. 7) who have healthy condition was participated this study, we collected data of muscle activation using by EMG from upper trapezius(Tr), deltoid middle fiber(De), extensor digitorum(Ed), first dorsal interosseous(Di) during participants was performed click and drag according various mouse. Mouse shape was divided 4 level as follow shape 1 was very small, 2 was small, 3 was moderate, 4 was large. Data was analyzed ANOVA, independent t-test using by SPSS ver18.0. Results : There was significantly difference of muscle activation among each muscle according mouse shape in drag and click. In shape 1, 4, there was significantly difference of muscle activation of Tr, De, Ed between drag and click except Di. In shape 2, 4, there was significantly difference of muscle activation of all muscle between drag and click. Conclusion : We knew that extensor digitroum showed more higher muscle activation than other muscle in drag, first dorsal interoseous showed more higher muscle activation that other muscle in click. We suggest that mouse shape was very important factor in order to prevent skeletal muscular disorder for computer user, and mouse shape can reduce muscle fatigue during computer work.

• PNF and Movement
• /
• 제14권3호
• /
• pp.195-202
• /
• 2016

Purpose: This study investigates the influence of applying proprioceptive neuromuscular facilitation (PNF) leg patterns on the activation of three types of mesogluteal fibers at the opposite side. Methods: The target subjects of this study were 19 healthy men who voluntarily agreed to participate in the program. For all subjects, the following four PNF leg patterns were applied while they were in a standing position, and then, the activation of the gluteus medius at the opposite side was measured: (1) flexion abduction internal rotation pattern, (2) flexion adduction external rotation pattern, (3) extension abduction internal rotation pattern, and (4) extension adduction external rotation pattern. In each position, a pattern was executed three times in 5 s, and the average of each measurement was used for the statistics. Results: Among the PNF leg patterns, the activation of the mesogluteal fibers at the opposite side was most significant under the application of the extension abduction internal rotation pattern. The activation of the back muscular fiber was significantly high under the application of the flexion abduction internal rotation pattern. Conclusion: Selective application of PNF leg patterns can improve the functional activity of each mesogluteal fiber.

• 시스템엔지니어링학술지
• /
• 제20권spc1호
• /
• pp.76-89
• /
• 2024

This study investigates the impact of standardized underground logistics conainers on lower body muscle activation during warehouse tasks, comparing conventional roll containers(A type) with newly developed ones(B type). Through a detailed experimental setup involving electromyography(EMG) and tensiomyography(TMG), muscle activities of the lower limbs were quantitatively analyzed during loading unloading and transporting tasks. Results indicated no significant difference in muscle activation patters between the two rolltainer types, suggesting that the dimensions of these containers do not critically affect the muscular strain and workload. Furthermore, the TMG analysis revealed that muscle contraction velocity(Vc) increased in certain muscles when using the B-type rolltainer, indicating a potential for more efficient muscle engagement without increasing fatigue. This research underscores the importance of ergonomic considerations in the design of logistics equipment and suggests that further studies should focus on optimizing the interaction between human operators and logistical systems to enhance safety and efficiency in warehouse operations.

• 한국운동역학회지
• /
• 제30권4호
• /
• pp.285-291
• /
• 2020

Objective: Shoes midsole are crucial for reducing impact forces on the lower extremity when someone is running. Previous studies report that the cushioning of running shoes make it possible to use less muscular energies. However, the well cushioned shoes result in energy loss as the shoe midsole is compressed. Cushioning reduces the load on the body, it also results in the use of more muscle energy to create propulsion force. The purpose of this study was to investigate the effect of the difference of shoe hardness & resilience on the running. Method: Shoes midsole are crucial for reducing impact forces on the lower extremity when someone is running. Previous studies report that the cushioning of running shoes make it possible to use less muscular energies. However, the well cushioned shoes result in energy loss as the shoe midsole is compressed. Cushioning reduces the load on the body, it also results in the use of more muscle energy to create propulsion force. The purpose of this study was to investigate the effect of the difference of shoe hardness & resilience on the running. Results: In vastus lateralis muscle Activation, Type 55 were significantly higher for Type 50 and X (p=0.019, p=0.045). In Gluteus Maximus muscle activation, Type 55 was significantly lower for type 50 (p=0.005). In loading late, Type 55 and X were significantly higher for type 45 (p=0.008, p=0.006). Conclusion: The components of a shoe are very complex, and there can be many differences in manufacturing as well. Although some differences can be found in the biomechanical variables of the high elastic midsole, it is difficult to interpret the performance enhancement and injury prevention.

• Physical Therapy Rehabilitation Science
• /
• 제11권1호
• /
• pp.88-96
• /
• 2022

Objective: Purpose of this study was to compare muscle activity ratio of multifidus to erector spinalis according to various cueing including tactile stimulation to provide an effective strategy to provide verbal and tactile feedback during exercise to provoke multifidus muscle activation. Design: Cross-sectional study. Methods: Participants of this study included 28 healthy adults. Muscle activities of the multifidus and erector spinalis were measured while the participants performed tasks according to the three different methods of verbal cueing and three different tactile stimulation. Surface EMG was used to measure the muscular activity of the muscles during all the tasks. Results: Tactile stimulation to abdomen and lumbar vertebrae showed no significant difference in the muscle activity ratio (p>0.05). However, muscle activity ratio of the multifidus in relation to the erector spinalis was increased when subjects were given verbal instructions to make lumbar curvature with little force and to make lumbar curvature while pulling navel (p<0.05). However, it was decreased when they were provided with verbal instruction to make lumbar curvature with strong force (p<0.05). Conclusions: According to the results, proper verbal instruction was an effective tool to increase the muscular activity of multifidus. This study aimed to find and provide the most appropriate verbal cueing while doing exercises to activate multifidus.