• 동의생리병리학회지
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• 제23권2호
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• pp.405-411
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• 2009

This study evaluated the effects of Puerariae Radix on the skeletal muscle atrophy, Muscle atrophy was induced by the sciatic nerve transection in Sprague-Dawley rats, then aqueous-extract of Puerariae Radix was administered for 12 days, Muscle wet weight was measured in soleus, plantaris, and medial gastrocnemius. Muscle fiber type was classified by MHCf immunohistochemistry. Muscle fiber type proportion and cross section area of muscle fiber also was observed in medial gastrocnemius. Bax and Bcl-2 expressions in medial gastrocnemius of the damaged hind limb were evaluated with immunohistochemistry. The results are as follows; Puerariae Radix attenuated muscle atrophy in soleus of the sciatic nerve transectioned rats, but there was statistic significance. Puerariae Radix attenuated significantly atrophy in plantaris at 12 days and in medial gastrocnemius at 8 days and 12 days. Puerariae Radix improved histology of the atrophic changes and increased significantly cross section areas of type-I and type-II muscle fibers in medial gastrocnemius of the sciatic nerve transectioned rats. Puerariae Radix did not affect to muscle fiber type proportion in medial gastrocnemius of the sciatic nerve transectioned rats. Puerariae Radix attenuated significantly Bax positive nuclei but did not affect to Bcl-2 positive muscle fibers in medial gastrocnemius of the sciatic nerve transectioned rats.According to above results, Puerariae Radix may have an anti-atrophy effect on the denervated skeletal muscle through anti-apoptotic effects on muscle fibers.

• 대한의용생체공학회:의공학회지
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• 제21권2호
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• pp.189-201
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• 2000

Mechanical factors in the human body are considered to play a dominant role in low back problems. Various spinal structures. including muscles, act in unison to resist the external load. An estimation of the muscle forces in this structure requires a knowledge of the orientation, location and area of cross-section of the muscles to complete the formulation of a truly three-dimensional mathematical model of the spine. The geometric parameters which are calculated were the line of action, the centroid and physiologic area of cross-section of each muscle as a function of the spinal level. This geometric data were obtained from CT scans of 11 subjects participating in this study.

• The Journal of Korean Physical Therapy
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• 제24권2호
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• pp.66-72
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• 2012

Purpose: The purpose of this study is to explore the importance of the image distortion correction in the cross sectional area measurement for the iliopsas muscle, tensor fasciae latae muscle, gluteus maximus muscle and the knee extensor muscles, by using (magnetic resonance imaging) MRI. Methods: This study was performed using an open 0.32T MRI system. To estimate the image distortion, T1 images for an AAPM homogeneity/linearity phantom were acquired, and the region in which the maximum geometric distortion was less than or equal to the pixel size (1.6 mm) of the images, it was defined as the distortion correction-free region. The T2 images for a human subject's pelvis and thigh in normal positions were obtained. Then, after the regions of interest in the pelvis and thigh were moved into the distortion correction-free region, T2 images for the pelvis and thigh were scanned with the same imaging parameters used in the previous T2 imaging. The cross-sectional areas were measured in the two T2 images that were obtained in the normal position, and the distortion correction-free region, as well as the area error caused by geometric image distortion was calculated. Results: The geometrical distortion is gradually increased, from the magnet center to the outer region, in axial and coronal plane. The cross-sectional area error of gluteus maximus muscle and the knee extensors was as high as 9.27% and 3.16% in before and after distortion correction, respectively. Conclusion: The cross-sectional area of the muscles that suffered from the geometrical distortion is necessary to correct for the estimation of the intervention.

• 한국식품과학회지
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• 제9권4호
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• pp.271-276
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• 1977

한우(韓牛)의 round muscke에 papain을 농도별(濃度別)로 증가(增加)시켜서 longitudinal section과 cross section하여 결체조직(結締組織)의 collagenous fibre와 elastic fibre를 각각(各各) 염색(染色)하여 현미경으로 관찰한 결과(結果)는 다음과 같다. 1. 효소(酵素) 농도(濃度)의 증가(增加)에 따라 비정형 상태로 뭉쳐져 있는 collagenous fibre는 점점 풀어지는 상태(狀態)를 지나 후에는 붕괴되며 또한 염색성(染色性)이 현저하게 약해졌다. 2. elastic fibre의 방향(方向)은 muscle fibre에 평행(平行)하게 존재(存在)하며 효소(酵素) 농도(濃度)의 증가(增加)에 따라 점차 탄성(彈性)을 잃고 loose헤지며 직선적(直線的)으로 보이고 fragment되는 것도 현저하다. 3. collagenous fibre와 elastic fibre 모두 효소(酵素) 처리구(處理區)가 대조구(對照區)에 비하여 조직변화(組織變化)가 현저하다.

• 대한물리의학회지
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• 제5권2호
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• pp.233-243
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• 2010

Purpose : This study aimed to examine the effects of sling exercise therapy on the relief of low back pain and the cross-sectional area change of lumbar muscles by CT analysis for chronic low back pain patients. Methods : Forty-one patients with diagnosis of chronic low back pain was divided sling exercise therapy group(SEG) 19 subjects and conservative physical therapy group(CPG) 22 subjects. we randomized and treated them(subjects) for 12 weeks. As using visual analogue scale(VAS), we evaluated recovery accuracy of pain, and for investigating cross-section area change of lumbar muscles for before and after treatment we used computed tomography(CT). Results : This study were summarized as follows : 1) As treatment period, in each compared testing on VAS of SEG and CPG, the both group was significantly different(p<.05). 2) Compared testing on VAS of between-subject groups(SEG and CPG) were not significantly different(p>.05). 3) At SEG of before and after treatment, in compared testing on cross-section area size of lumbar muscles, All muscles of psoas major, quadratus lumborum, erector spinae and multifidus were significantly different(p<.05). At CPG, psoas major was significantly different(p<.05). But other muscles were not significantly different(p>.05). Conclusion : From this result, both sling exercise therapy and the conservative physical therapy are effect on decrease of pain for chronic low back pain patients. but Increasing of the cross-section area being proportioned with muscular strengthening of low back muscle is noticeable difference in SEG. Therefore, sling exercise therapy have affect both decreasing of pain for chronic low back pain patients and lumbar muscles strengthening.

• 한국식품과학회지
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• 제8권4호
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• pp.207-211
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• 1976

오징어의 동부근육(胴部筋肉)을 각종방법(各種方法)으로 처리(處理)하여 cross section하고 Van Gieson방법(方法)으로 염색(染色)하여 박막상(薄膜狀)의 표피층(表皮層)이 박리(剝離)되는 상태(狀態)를 현미경으로 관찰한 결과(結果)는 다음과 같다. 1. 일반적으로 사용되는 온탕법(溫湯法)에서 1, 2, 3층(層)은 제거(除去)되지만 4층(層)은 거의 제거(除去)되지 않았다. 2. 열탕처리(熱湯處理)에 의해서 근섬유(筋纖維) 조직(組織)에 근섬유(筋纖維)의 방향(方向)으로 많은 균열 현상을 보였다. 3. 초산소오다가 질산소다. 황산(黃酸)소오다 및 주석산(酒石酸)소오다 처리(處理)보다 박리상태(剝離狀態)가 현저히 우수하였으며 특(特)히 고농도(高濃度)에서 현저하고 약품처리(藥品處理) 방법중(方法中) 가장 우수한 효과(?果)를 보였다. 4. 단백(蛋白) 분해효소(分解酵素) 처리시(處理時) 표피(表皮)의 1, 2, 3, 4, 층(層)은 완전히 제거(除去)되며 특(特)히 표피(表皮)와 육(肉)과의 경계면 섬유(纖維)를 현저하게 팽윤(澎潤)시켰다. 5. 효소(酵素)와 초산소오다로 처리(處理)하는 2종류(種類)의 박피법(剝皮法)은 유효(有效)하다는 것을 확인(確認)하였다.

• The Journal of Korean Physical Therapy
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• 제22권3호
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• pp.9-15
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• 2010

Purpose: To evaluate, in patients with degenerative disc disease (DDD), the efficacy of using spinal stabilizing exercises for the reversal? of atrophy of the multifidus and psoas major, reductions in pain and disability, and for increases in paraspinal muscle strength. Methods: Nineteen patients diagnosed with DDD participated for 10 weeks in a spinal stabilization exercise program. Pain and disability were measured before and after exercise using, respectively, a visual analogue scale (VAS) and the Oswestry Disability Index (ODI). Paraspinal muscular strength in four directions was evaluated using CENTAUR. Both before and after exercise we used computed tomography (CT) too measure cross-sectional areas (CSAs) of both the left and right multifidus and the psoas major at the upper & lower endplate of L4. Results: After 10 weeks of a spinal stabilization exercise program, pain was significantly decreased from $5.7{\pm}0.9$ to $2.5{\pm}0.9$ (p<0.01); the ODI score decreased from $16.7{\pm}4.9$ to $7.3{\pm}3.1$. Paraspinal muscle strength was significantly increased (p<0.01) and the CSAs of the left and right multifidus and psoas major muscles were significantly increased (p<0.01). Conclusion: Spinal stabilization exercise is effective in reversing atrophy in DDD patients, in reducing pain and disability, and in increasing paraspinal muscle strength. It is an effective treatment foro aiding rehabilitation in these cases.

• 한방재활의학과학회지
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• 제27권4호
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• pp.1-9
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• 2017

Objectives Oxidative stress, in which antioxidant proteins and scavenger protection are overwhelmed by reactive oxygen species (ROS) production, is recognized as one of central causes of disuse muscle atrophy. In this study, the hypothesis that oral treatment with Dangguibohyul-tang (Dangguibuxuetang) could attenuate immobilization-induced skeletal muscle atrophy was tested. Methods The hindlimb immobilization was performed with casting tape to keep the left ankle joint in a fully extended position. The Rats in Dangguibohyul-tang treated group (DGBHT) (n=10) were orally administrated Dangguibohyul-tang water extract, and rats of Control group (n=10) were given with saline only. After 2 weeks of immobilization, the morphology of right and left gastrocnemius muscles in both DGBHT and Control groups were assessed by hematoxylin and eosin staining. Results Dangguibohyul-tang water extract represented the significant protective effects against the reductions of the left gastrocnemius muscles weight and average cross section area to compared with Control group. Moreover, the treatment with Dangguibohyul-tang extract significantly enhanced the Cu/Zn-SOD activities in gastrocnemius muscle compared with Control group. Conclusions Thses results suggest that Dangguibohyul-tang has protective effects against immobilization-induced muscle atrophy by increasing the Cu/Zn-SOD activities in gastrocnemius muscle.

• 대한통합의학회지
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• 제5권4호
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• pp.49-55
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• 2017

Purpose : In this study we tested the hypothesis that eucommia ulmoides (EU) extract would improve disuse-induced muscle atrophy following hindlimb immobilization. Method : The hindlimb immobilization was performed with casting tape to keep the left ankle joint in a fully extended position. The rats in EU treated group were orally administrated with eucommia ulmoides water extract. After 2 weeks of immobilization, all animals were sacrificed, and the whole gastrocnemius muscles were dissected from both legs. The morphology of right and left gastrocnemius muscles in both EU and control groups were assessed by hematoxylin and eosin staining. Results : Eucommia ulmoides extract represented the significant protective effects against the reductions of the gastrocnemius muscles weight and average cross section area to compared with Control group. Conclusion : Eucommia ulmoides has protective effects against immobilization induced muscle atrophy.

• 대한한의학방제학회지
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• 제24권3호
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• pp.153-161
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• 2016

Chronic alcoholic myopathy is one of the most common skeletal muscle disorders. It is characterized by a reduction in the entire skeletal musculature, skeletal muscle weakness, and difficulties in gait. Patients with alcoholic hepatitis and cirrhosis have severe muscle loss that contributes to worsening outcome. Although the myopathy selectively affects Type II (fast twitch, glycolytic, anaerobic) skeletal muscle fibers, total skeletal musculature is reduced. The severity of the muscle atrophy is proportional to the duration and amount of alcohol consumed and leads to decreased muscle strength. The mechanisms for the myopathy are generally unknown but it is not due to overt nutritional deficiency, nor due to either neuropathy or severe liver disease. Skeletal muscle mass and protein content are maintained by a balance between protein synthesis and breakdown and in vivo animal models studies have shown that ethanol inhibits skeletal muscle protein synthesis. Daekumeumja is a traditional Korean medicine that is widely employed to treat various alcohol-induced diseases. Muscle diseases are often related to liver diseases and conditions. The main objective of this study was to assess that Daekumeumja extract could have protective effect against alcoholic myopathy in a Sprague-Dawley rat model. Rats were orally given 25% ethanol (5ml/kg, body weight) for 8 weeks. After 30 minutes, rats were administrated with Daekumeumja extract. Controls were similarly administrated with the vehicle alone. The weights of gastrocnemius, soleus and plantaris muscles were assessed and the morphologic changes of gastrocnemius and plantaris muscles were also assessed by hematoxylin and eosin staining. In results, The muscles from ethanol treated rats displayed a significant reduction in muscle weight and average cross section area compared to Normal group. Daekumeumja extract treated group showed increased muscle weight and muscle fiber compared to the ethanol treated group. It was concluded that Daekumeumja extract showed ameliorating effects on chronic alcohol myopathy in skeletal muscle.