• Proceedings of the Optical Society of Korea Conference
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• 2009.02a
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• pp.297-298
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• 2009

파장 분할 방식의 광섬유 격자 센서의 멀티플렉싱 능력을 향상시키기 위하여 이중 격자의 파장 간격 변조로 구분되는 다중 광섬유 격자 센서 시스템을 제안하였으며, 서로 다른 파장 간격을 가지는 센서의 상태와 위치를 정확히 구별할 수 있는 방법을 연구하고 씨뮬레이션을 통하여 확인하였다. 또한 변형력 하에서 광섬유격자 센서들간의 스펙트럼 중첩으로 불명확한 브래그 피크를 최급 강하법(steepest descent method) 알고리즘을 사용하여 약 3 pm이내의 표준편차로 결정할 수 있었다.

• Analytical Science and Technology
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• v.27 no.3
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• pp.121-139
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• 2014

In this review, we will discuss aptamer technologies including in vitro selection, signal transduction mechanisms, and designing aptamers and aptazyme for label-free biosensors and catalysts. Dye-displacement, a typical label-less method, is described here which allows avoiding relatively complex labeling steps and extending this application to any aptamers without specific conformational changes, in a more simple, sensitive and cost effective way. We will also describe most recent and advanced technologies of signaling aptamer and aptazyme for the various analytical and clinical applications. Quantum dot biosensor (QDB) is explained in detail covering designing and adaptations for multiplexed protein detection. Application to aptamer array utilizing self-assembled signaling aptamer DNA tile and the novel methods that can directly select smart aptamer or aptazyme experimentally and computationally will also be finally discussed, respectively.

• Biotechnology and Bioprocess Engineering:BBE
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• v.11 no.5
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• pp.455-461
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• 2006

In this study, we have described a method for the fabrication of a protein chip on silicon substrate using hydrophobic thin film and microfluidic channels, for the simultaneous detection of multiple targets in samples. The use of hydrophobic thin film provides for a physical, chemical, and biological barrier for protein patterning. The microfluidic channels create four protein patterned strips on the silicon surfaces with a high signal-to-noise ratio. The feasibility of the protein chips was determined in order to discriminate between each protein interaction in a mixture sample that included biotin, ovalbumin, hepatitis B antigen, and hepatitis C antigen. In the fabrication of the multiplexed assay system, the utilization of the hydrophobic thin film and the microfluidic networks constitutes a more convenient method for the development of biosensors or biochips. This technique may be applicable to the simultaneous evaluation of multiple protein-protein interactions.

• Proceedings of the Korean Vacuum Society Conference
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• 2011.02a
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• pp.431-431
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• 2011

Graphene, two dimensional sheet of sp2-hybridized carbon, has attracted an enormous amount of interest due to excellent electrical, chemical and mechanical properties for the application of transparent conducting films, clean energy devices, field-effect transistors, optoelectronic devices and chemical sensors. Especially, graphene is promising candidate to detect the gas molecules and biomolecules due to the large specific surface area and signal-to-noise ratios. Despite of importance to the disease diagnosis, there are a few reports to demonstrate the graphene- and rGO-FET for biological sensors and the sensing mechanism are not fully understood. Here we describe scalable and facile fabrication of rGO-FET with the capability of label-free, ultrasensitive electrical detection of a cancer biomarker, prostate specific antigen/${\alpha}1$-antichymotrypsin (PSA-ACT) complex, in which the ultrathin rGO sensing channel was simply formed by a uniform self-assembly of two-dimensional rGO nanosheets on aminated pattern generated by inkjet printing. Sensing characteristics of rGO-FET immunosensor showed the highly precise, reliable, and linear shift in the Dirac point with the analyte concentration of PSA-ACT complex and extremely low detection limit as low as 1 fg/ml. We further analyzed the charge doping mechanism, which is the change in the charge carrier in the rGO channel varying by the concentration of biomolecules. Amenability of solution-based scalable fabrication and extremely high performance may enable rGO-FET device as a versatile multiplexed diagnostic biosensor for disease biomarkers.

• Journal of The Korean Astronomical Society
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• v.29 no.spc1
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• pp.389-390
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• 1996

The CCDs are widely used in astronomical observations either in direct imaging use or spectroscopic mode. However, the areas of available sensors are too small for large imaging format. One possibility to obtain large detection area is to assemble mosaics of CCD, and drive them simultaneously. Parallel driving of many CCDs together rules out the possibility of individual tuning; however, such optimisation is very important, when the ultimate low light level performance is required, particularly for new, or mixed devices. In this work, a new concept is explored for an entirely novel approach, where the drive waveforms are multiplexed and interleaved. This simultaneously reduces the number of leadout connections and permits individual optimisation efficiently. The digital controller can be designed within a single EPLD (Erasable Programmable Logic Device) chip produced by a CAD software package, where most of the digital controller circuits are integrated. This method can minimise the component. count., and improve the system efficiency greatly, based on earlier works by Han et a1. (1996, 1994). The system software has an open architecture to permit convenient modification by the user, to fit their specific purposes. Some variable system control parameters can be selected by a user with a wider range of choice. The digital controller design concept allows great flexibility of system parameters by the software, specifically for the compatibility to deal with any number of mixed CCDs, and in any format, within the practical limit.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.18 no.9
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• pp.1404-1411
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• 1993

Unlike voice communication, data transmission, can be seriously affected by transient channel impairments. In some cases, timing synchronization between the transmitter and the receiver may not be recovered in the presence of these kinds of impairments without a forced reinitialization process. Therefore, it is highly desirable for data communication equipment to have an efficient timing loss detector for robust recovery. In this paper, one such detector is proposed for data transceivers haying a secondary channel embedded in the main channel. A known sequence multiplexed with the secondary channel data is repeatedly sent through the embedded secondary channel. For continuous watch-dog like operation, the detection is sequentially performed based on a modified up/down counter scheme. The performance of the proposed detector is analytically evaluated In closed form.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.35 no.12C
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• pp.1044-1051
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• 2010

In this paper, we propose an efficient soft-output signal detection method for spatially multiplexed multiple input multiple output (MIMO) systems. The proposed method is based on the ordered successive interference cancellation (OSIC) algorithm, but it significantly improves the performance of the original OSIC algorithm by solving the error propagation problem. The proposed method combines this enhanced OSIC (ESIC) algorithm with a multiple ordering technique in a very efficient way. As a result, the log likelihood ratio (LLR) values can be computed by using a very small set of candidate symbol vectors. The proposed method has been implemented with a $0.13{\mu}m$ CMOS technology for a $4{\times}4$ 16-QAM MIMO system. The simulation and implementation results show that the proposed detector provides a very good solution in terms of performance and hardware complexity.

• Bulletin of the Korean Chemical Society
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• v.35 no.5
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• pp.1279-1284
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• 2014

The binding of TATA-binding protein (TBP) to the TATA-box containing promoter region is aided by many other transcriptional factors including TFIIA and TFIIB. The mechanistic insight into the assembly of RNA polymerase II preinitation complex (PIC) has been gained by either directly altering a function of target protein or perturbing molecular interactions using drugs, RNAi, or aptamers. Aptamers have been found particularly useful for studying a role of a subset of PIC on transcription for their ability to inhibit specific molecular interactions. One major hurdle to the wide use of aptamers as specific inhibitors arises from the difficulty with traditional assays to validate and determine specificity, affinity, and binding epitopes for aptamers against targets. Here, using a technique called the bio-layer interferometry (BLI) designed for a label-free, real-time, and multiplexed detection of molecular interactions, we studied the assembly of a subset of PIC, TBP binding to TATA DNA, and two distinct classes of aptamers against TPB in regard to their ability to inhibit TBP binding to TFIIA or TATA DNA. Using BLI, we measured not only equilibrium binding constants ($K_D$), which were overall in close agreement with those obtained by electrophoretic mobility shift assay, but also kinetic constants of binding ($k_{on}$ and $k_{off}$), differentiating aptamers of comparable KDs by their difference in binding kinetics. The assay developed in this study can readily be adopted for high throughput validation of candidate aptamers for specificity, affinity, and epitopes, providing both equilibrium and kinetic information for aptamer interaction with targets.

• Biomedical Science Letters
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• v.21 no.1
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• pp.9-14
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• 2015

Mouse hepatitis virus (MHV) is a major pathogen in laboratory mice that usually leads to fatal diseases, such as hepatitis, multiple sclerosis, encephalitis, and respiratory disease. MHV has a high infection rate, and it needs to be detected as soon as possible to prevent its spread to other facilities. However, MHV detection by enzyme-linked immunosorbent assay (ELISA) often gives false positives; thus, it is very important that the results are confirmed as true positives in the early infection stage or distinguished as false positives with more accurate, reliable methods. Under microbiological screening, MHV ELISA-positive mice were found in four GFP-tagging transgenic mice. To verify the detection of the MHV antigen directly, reverse transcription polymerase chain reaction (RT-PCR) was performed, and the mice were determined to be MHV negative. Additional serum antibody-based screening was conducted with three different ELISA kits, and multiplexed fluorometric immunoassay (MFIA) was performed to confirm their accuracy/sensitivity. In brief, the ELISA kit for A59 nucleocapsid protein (MHV-A59N) revealed MHV ELISA positivity, while other ELISA kits (MHV-S lysate and MHV-JHM lysate) demonstrated MHV negativity. In MFIA, only the test for the recombinant A59 nucleocapsid antigen was MHV positive, which was consistent with the ELISA results. These results suggest that the ELISA kit with the recombinant A59 nucleocapsid antigen might induce non-specific MHV ELISA positivity and that confirmation is therefore essential.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.42 no.12
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• pp.103-110
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• 2005

The performance of maximum likelihood(ML) detection for the given channel is analyzed in spatially multiplexed MIMO system. In order to obtain the vector symbol error rate, we define error vectors which represent the geometrical relation between lattice points. The properties of error vectors are analyzed to show that all lattice points in infinite lattice almost surely have four nearest neighbors after random channel transformation. Using this information and minimum distance obtained by the modified sphere decoding algorithm, we formulate the analytical performance of vector symbol error over the given channel. To verify the result, we simulate ML performance over various random channel which are classified into three categories: unitary channel, dense channel, and sparse channel. From the simulation results, it is verified that the derived analytical result gives a good approximation about the performance of ML detector over the all random MIMO channels.