• Journal of Microbiology and Biotechnology
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• 제31권2호
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• pp.197-206
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• 2021

microRNA-361-3p (miR-361-3p) is involved in the carcinogenesis of oral cancer and pancreatic catheter adenocarcinoma, and has anti-carcinogenic effects on non-small cell lung cancer (NSCLC). However, its effect on multiple myeloma (MM) is less reported. Here, we found that upregulating the expression of miR-361-3p inhibited MM cell viability and promoted MM apoptosis. We measured expressions of tumor necrosis factor receptor-associated factor 6 (TRAF6) and miR-361-3p in MM cells and detected the viability, colony formation rate, and apoptosis of MM cells. In addition, we measured expressions of apoptosis-related genes Bcl-2, Bax, and Cleaved caspase-3 (C caspase-3). The binding site between miR-361-3p and TRAF6 was predicted by TargetScan. Our results showed that miR-361-3p was low expressed in the plasma of MM patients and cell lines, while its overexpression inhibited viability and colony formation of MM cells and increased the cell apoptosis. Furthermore, TRAF6, which was predicted to be a target gene of miR-361-3p, was high-expressed in the plasma of patients and cell lines with MM. Rescue experiments demonstrated that the effect of TRAF6 on MM cells was opposite to that of miR-361-3p. Upregulation of miR-361-3p induced apoptosis and inhibited the proliferation of MM cells through targeting TRAF6, suggesting that miR-361-3p might be a potential target for MM therapy.

• 원예과학기술지
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• 제32권3호
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• pp.375-381
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• 2014

Transgenic potato was generated to express recombinant 5 repeated ${\beta}$-amyloid ($A{\beta}$) peptides, potential antigens to be applied as a preventive accine for Alzheimer's disease using Agrobacterium mediated transformation. The $A{\beta}$ peptides were fused to the human IgG Fc fragment enhancing protein and KDEL, which is the endoplasmic reticulum (ER) retention signal ($5A{\beta}$-FcK). The $5A{\beta}$-FcK, was expressed under the control of the duplicated 35S promoter. PCR analysis confirmed the presence of the transgene in several transgenic potato lines. Southern blot analysis showed only a single gene copy number in transgenic line 22, whereas multiple gene copy numbers were shown for transgenic lines 31 and 44. Northern blot analysis showed that line 22 had stronger mRNA levels when compared to lines 31 and 44. Immunoblot analysis confirmed that the $5A{\beta}$-FcK protein was expressed in the transgenic potato plant. These results indicate that $5A{\beta}$ fused to Fc can be expressed in potato plants.

• 전자공학회논문지D
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• 제35D권12호
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• pp.1-11
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• 1998

고속펄스 전송을 위한 다중결합된 마이크로스트립 신호버스에서 제일 근접한 선로상에 유기되는 누화피크치를 최소화시키기 위해 선로자태를 합성시키는 방법을 제안하였다. 평행하게 배열된 데이터버스에서 구동선로로부터의 누화에너지는 가장 근접한 선로에 거의 집중되므로 반사파 제어를 위한 최적화기법을 통해 선로간의 평균간격을 늘리는 방법을 채택하였다. 입출력 파형예측을 위해 일반화된 S-행렬 기법을 적용하였으며 선로간의 평균간격을 늘리기 위해 합성된 다양한 형태의 비일정선로의 누화특성을 비교 분석하였다. 그 결과 펄스가 갖는 주파수 범위내에서 스펙트럼을 고르게 반사시키게 하는, dip을 갖는 쳬비셰프형 테이퍼가 입출력 파형의 보전성에 큰 영향을 주지 않는 범위내에서 누화피크치를 최소로 함을 볼 수 있었다.

• Journal of Electrical Engineering and Technology
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• 제9권2호
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• pp.415-422
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• 2014

This paper presents a new approach to solve the multi area unit commitment problem (MAUCP) using an evolutionary programming based particle swarm optimization (EPPSO) method. The objective of this paper is to determine the optimal or near optimal commitment schedule for generating units located in multiple areas that are interconnected via tie lines. The evolutionary programming based particle swarm optimization method is used to solve multi area unit commitment problem, allocated generation for each area and find the operating cost of generation for each hour. Joint operation of generation resources can result in significant operational cost savings. Power transfer between the areas through the tie lines depends upon the operating cost of generation at each hour and tie line transfer limits. Case study of four areas with different load pattern each containing 7 units (NTPS) and 26 units connected via tie lines have been taken for analysis. Numerical results showed comparing the operating cost using evolutionary programming-based particle swarm optimization method with conventional dynamic programming (DP), evolutionary programming (EP), and particle swarm optimization (PSO) method. Experimental results show that the application of this evolutionary programming based particle swarm optimization method has the potential to solve multi area unit commitment problem with lesser computation time.

• 센서학회지
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• 제22권3호
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• pp.212-218
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• 2013

In this paper, we introduce a new method to reduce the inter-channel crosstalk in visible light communication (VLC) systems using the synchronizing pulses transmitted through the power lines. Synchronizing pulses are simultaneously transmitted to multiple VLC transmitters and receivers through the 220V power line. Each VLC transmitter modulates an LED and each VLC receiver demodulates the signal light in the time slot that is allocated with reference to the synchronizing pulses. This method is very simple and effective to prevent the inter-channel crosstalk in VLC systems for sensor networks because every VLC system can easily get the synchronizing pulses from the nearby power line.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2008년도 추계학술대회논문집
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• pp.212-213
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• 2008

Ultrasonic fiber sensor is more cost-effective than optical fiber sensor using light. However, optical fiber lines are separately used for sensing. Thus, if it is applied for huge system, it is need many lines of the sensor systems. To overcome this point, novel ultrasonic sensor systems considering wave cancellation and modulation were newly developed. By using this schema, reference-free sensor system can be developed. By using ultrasonic waves of different excitation frequencies, multiple input-signal output sensor system was also developed by applying spectrum analyses. Using the array type sensor system, the leakage of liquid and its evaporation can be monitored successfully.

• 대한산업공학회지
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• 제16권2호
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• pp.81-89
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• 1990

Transfer pricing mechanism is applied to the problem of input buffer size in the context of interfacing a flexible manufacturing system with multiple following production lines. The size of the input buffers can be determined economically by using non-linear transfer pricing either in a decentralized organization or in a centralized organization. Under the certain conditions, input buffer size determined from this non-linear transfer pricing is more economical than the traditional economic lot size model. The benefit comes from transferring part of FMS' inventory to the following production lines. And this non-linear transfer pricing makes sense if the FMS' unit inventory holding cost is high enough.

• 한국경영과학회:학술대회논문집
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• 대한산업공학회/한국경영과학회 1996년도 춘계공동학술대회논문집; 공군사관학교, 청주; 26-27 Apr. 1996
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• pp.365-368
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• 1996

The setting of the factory under this study is as the following. There are multiple assembly lines. Each line can handle any of given set of products. Furthermore each line is capable of assembling several products concurrently, i.e. mixed model assembly line. An incoming production order is characterized by its due-date, product type and quantity. Under this setting we first have to select the starting time and the place(assembly line) for an order to be processed. We also have to devise a way to control orders to be manufactured as scheduled. Finally there should be a mean to reschedule orders when something unexpected happens. This paper offers a scheme that provides the above mentioned necessities. It also provides a case where the scheme is applied.

• 산업기술연구
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• 제28권B호
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• pp.129-133
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• 2008

A method to reduce seam line artifact in spatial compounding of ultrasonic images is presented. Spatial compounding is a speckle reducing imaging technique in which a number of ultrasound images of a given target that have been obtained from multiple view angles are combined into a single compounded image by combining the data received from each data point in the compounded image. Since different view angle results in different view area, and the images of different view arms are combined into an image, the compounded image consists of regions with different signal to noise ratio, and the boundary lines between these regions are visible as seam lines in the compounded images. In this paper, we present an algorithm that reduces the visibility of this seam line in the spatially compounded images. Design procedure for a FIH filter is described and the results of applying the filter to in-vivo ultrasonic images are analyzed.

• 식물조직배양학회지
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• 제28권3호
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• pp.147-151
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• 2001

Explants of Lactuce sativa cultivar, chungchima, were co-cultivated with Agrobacterium tumefaciences LBA4404, EHA101 strains containing nptll gene and ferritin gene encoding iron storage protein from soybean for transformation. Through initial selection of regenerated explants by culturing on a kanamycin and carbenicillin containing MS medium, multiple shoots were obtained after 2 months of culture. For a complementary step of selection, putative transgenic shoots were transferred to 1/2 MS basal medium supplemented with 100 mg/L kanamycin and 500 mg/L carbenicillin. The selected shoots were tested with PCR analysis using nptll, ferritin specific primers whether ferritin gene was introduced to genome of the plants. These results confirmed that produced the specific PCR bands in the putative transgenic lines. Additionally the Northern blot showed that transcripts of ferritin gene were detected in mature leaf of the transgenic lines. These results suggest that ferritin gene be successfully integrated and transcribed in the putative transgenic lettuce plants.