• Biomolecules & Therapeutics
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• 제29권1호
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• pp.83-89
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• 2021

Multiple system atrophy (MSA) is a neurodegenerative disease characterized by presence of α-synuclein-positive inclusions in the cytoplasm of oligodendrocytes. These glial cytoplasmic inclusions (GCIs) are considered an integral part of the pathogenesis of MSA, leading to demyelination and neuronal demise. What is most puzzling in the research fields of GCIs is the origin of α-synuclein aggregates in GCIs, since adult oligodendrocytes do not express high levels of α-synuclein. The most recent leading hypothesis is that GCIs form via transfer and accumulation of α-synuclein from neurons to oligodendrocytes. However, studies regarding this subject are limited due to the absence of proper human cell models, to demonstrate the entry and accumulation of neuronal α-synuclein in human oligodendrocytes. Here, we generated mature human oligodendrocytes that can take up neuronderived α-synuclein and form GCI-like inclusions. Mature human oligodendrocytes are derived from neural stem cells via "oligosphere" formation and then into oligodendrocytes, treating the cells with the proper differentiation factors at each step. In the final cell preparations, oligodendrocytes consist of the majority population, while some astrocytes and unidentified stem cell-like cells were present as well. When these cells were exposed to α-synuclein proteins secreted from neuron-like human neuroblastoma cells, oligodendrocytes developed perinuclear inclusion bodies with α-synuclein immunoreactivity, resembling GCIs, while the stem cell-like cells showed α-synuclein-positive, scattered puncta in the cytoplasm. In conclusion, we have established a human oligodendrocyte model for the study of GCI formation, and the characterization and use of this model might pave the way for understanding the pathogenesis of MSA.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 1999년도 봄 학술발표회 논문집
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• pp.276-286
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• 1999

A recently developed numerical method based on a volume integral formulation is developed for the effective accurate calculation of the stress intensity factors at the crack tips in unbounded isotropic solids in the presence of multiple anisotropic inclusions and cracks and subjected to external loads. In this paper, a detailed analysis of the stress intensity factors are carried out for an unbounded isotropic matrix containing an orthotropic cylindrical inclusion and a crack. The accuracy and effectiveness of the new method are examined through comparison with results obtained from analytical method and finite element method using ANSYS. It is demonstrated that this new method is very accurate and effective for solving plane elastostatic problems in unbounded solids containing anisotropic inclusions and cracks.

• 한국전산구조공학회논문집
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• 제12권3호
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• pp.465-474
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• 1999

서로 상호작용을 하는 다수의 이방성 함유체 및 균열(crack)을 포함하는 등방성 무한고체가 정적 무한하중을 받을 때 균열선단에서의 응력확대계수의 계산을 효과적으로 수행할 수 있는 수치해석 방법으로 체적 적분방정식법을 적용한다. 본 해석방법의 타당성과 우수성을 입증하기 위하여, 비교적 간단한 형태의 이방성을 나타나는 직교이방성 함유체와 균열이 포함된 무한고체가 무한하중을 받을 때 균열선단에서의 응력확대계수 계산을 수행하고, 상업용 유한요소법 코드인 ANSYS를 이용한 해석결과와 비교 검토하였다.

• 전산구조공학
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• 제10권2호
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• pp.213-223
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• 1997

탄성파의 변형 및 응력계산에 관한 연구는 비파괴검사를 비롯하여 광범위한 공학분야에서 중요한 역할을 하고 있다. 특히 파형의 산란문제가 많은 연구자들에 의해 다양한 방법으로 연구되고 있다. 실린더 또는 구와 같은 간단한 형상을 지닌 산란체에 대하여, 정상상태 탄성파의 산란문제의 해석은 해석적 기법을 이용한 연구가 가능하다. 하지만 임의의 형상을 갖는 산란체 또는 다수의 함유체에 대한 해석에는 수치해석방법이 요구된다. 예를 들면, 무한요소법 또는 Global-Local 유한요소법이라고 하는 혼성 유한요소법과 같은 특수한 유한요소법등이 개발되고 있다. 최근에는 경계요소법을 사용한 산란문제의 해석에 대한 연구가 진행되고 있다. 본 논문에서는 다수의 임의의 형상을 갖는 함유체, 공동 또는 크랙을 포함하고있는 무한고체에서의 일반적인 탄성동력학 문제를 해석하기 위해 새롭게 개발된 체적적분 방정식법을 소개한다. 또한 경계요소법을 사용하여 탄성파의 산란문제에 대한 수치해석을 수행하였으며, 이의 결과를 체적적분 방정식법의 결과와 비교 검토 하였다.

• 대한기계학회논문집A
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• 제23권6호
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• pp.968-978
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• 1999

The effective elastic properties of materials containing spherical inclusions were calculated by the elastic wave scattering theory. In the formulation additional scattering fields by the presence of random multiple scatterers that affects the effective properties were found by the single scattering approximation. In calculating the scattering fields the ensemble average on the displacements and strains inside the scatterer was found from the static approximation at long wavelength limit. The displacements were assumed to be equal to the incident field, while the strains were calculated by Eshelby's equivalent inclusion principle on the single inclusion problem. Four different models were considered and they reflected different degrees of multiple scattering effects based on the approximation introduced in the process of embedding the inclusion in the matrix. The expressions for the effective elastic constants were given in each model, and their relations to the results obtained from other scattering theory and elasticity theory were discussed. The theoretical predictions were compared with experimental results on the epoxy matrix composites containing tungsten particles of different sizes and volume fractions

• 응용통계연구
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• 제22권4호
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• pp.759-770
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• 2009

본 논문은 신호와 잡음이 혼합된 관측치로부터 신호 관측치를 분류하는 문제를 다룬다. 잡음은 가우시안 분포를 따르고 신호는 감마 분포를 따른다고 가정할 때 관측치의 분포는 가우시안과 감마의 혼합 분포를 따르게 된다. EM 알고리즘을 통해 혼합 모델의 모수를 추정하고 신호 및 잡음을 분류하는 것을 다중 가설 검정으로 간주하여 베이즈 오류를 바탕으로 분류를 위한 경계치를 설정한다. 제안하는 방법을 분광 데이터에 근거하여 철강 제품에서 개재물 유무를 검출하는 문제에 적용하였고 별도의 시뮬레이션 데이터를 통해 성능의 우수성을 보였다.

• 한국음향학회지
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• 제11권4호
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• pp.22-30
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• 1992

섬유강화 복합재료의 동탄성계수와 감쇠특성을 규명하기 위하여 랜덤하게 분포된 무한 실린더 형상의 산란계를 가진 메질내에서, 축방향으로 분극되어 조화운동을 하는 탄성파의 전파에 관하여 연구하였다. 단일 실린더에 대한 산란계수로부터 Lax의 준결정근사법을 이용하여 다중산란에 관한 이론을 유도하였고, 매질내에서의 파동전파특성을 내포하는 분산관계식을 얻었다. 다중 산란에 의한 실린더간의 상호작용을 수식화하기 위하여 필요한 실린더의 쌍분포함수는 몬테카를로 모의실험을 이용하여 구하였다. 수치적으로 구한 감쇠계수 및 유효전단강성을 주파수와 면적밀도의 함수로 제시하였다.

• 대한한방내과학회지
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• 제33권1호
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• pp.102-110
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• 2012

Multiple system atrophy (MSA) is a sporadic progressive neurodegenerative disorder characterized clinically by various combinations of parkinsonian, autonomic, cerebellar, or pyramidal signs and pathologically by cell loss, gliosis, and ${\alpha}$-synuclein-positive glial cytoplasmic inclusions in several brain and spinal cord structures. This is a clinical report about a 69-year-old female who had MSA treated by oriental medical treatment and evaluated by Unified Multiple System Atrophy Rating Scale (UMSARS). The patient was treated with herb medicine Chungsimyeonj-aeumgami(淸心蓮子飮加味), acupuncture, moxibustion and cupping. After treatment, the patient's symptoms improved meaningfully and the score decreased in UMSARS Part I, II. This suggests that oriental medical treatment could be effective to improve MSA patients' symptoms. It is necessary to have more observations and many cases of patients with MSA.

• 자원환경지질
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• 제20권1호
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• pp.1-18
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• 1987

The Cheolam silver deposits are emplaced along the fractures in breccia dike and the Hongjesa granite. Breccia dike contains fragments of late Cretaceous acidic volcanic rocks and other fragments of various rocks distributed in the mine area. Therefore it is presumed that the mineralization was taken place in later than late Cretaceous time. Mineral paragenesis is complicated by multiple episodes of fracturing. Six distinct depositional stages can be recognized: stage I, deposition of base metal sulfides; stage II, deposition of base metal sulfides and silver minerals; stage III, deposition of carbonates; stage IV, deposition of silver minerals and base metal sulfides; stage V, deposition of silver minerals; stage VI, deposition of barren quartz. Silver minerals from the deposits are native silver, acanthite, pyrargyrite, argentian tetrahedrite, stephanite, polybasite, pearceite, allargentum, antimonial silver and electrum. Fluid inclusion studies ware carried out for stage I, II, IV and VI quartz and stage III calcite. Homogenization temperatures for each stage are as follows: stage I, from $225^{\circ}$ to $360^{\circ}C$; stage II, from $145^{\circ}$ to $220^{\circ}C$; stage III, from $175^{\circ}$ to $240^{\circ}C$; stage IV, from $130^{\circ}$ to $185^{\circ}C$; stage VI, from $120^{\circ}$ to $145^{\circ}C$. Salinities of ore fluids were in the range of 4 and 10 wt.% equivalent NaCl over stage I and stage VI. Ore mineralogical data of each stage indicate that temperatures are within the range of homogenization temperature of fluid inclusions and sulfur fugacities declined steadily from $10^{-9.7}atm$. to $10^{-18.7}atm$. through stage I into stage V.

• The Plant Pathology Journal
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• 제21권3호
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• pp.252-257
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• 2005

In single infection of Tobacco mosaic virus-U1 (TMV­U1) or Pepper mild mottle virus (PMMoV), mosaic symptoms were produced on the chili pepper cultivars of 'Cheongyang' and 'Wangshilgun'. However, in cultivars of 'Manitta' and 'Bugang', no symptoms were occurred. In single infection of Pepper mottle virus (PepMoV), symptoms of mottle and malformation were produced on the tested cultivars of 'Manitta', 'Bugang', 'Cheongyang', and 'Wangshilgun'. In the cultivars of 'Cheongyang' and 'Wangshilgun', synergistic symptoms of stunt and lethal death were induced by mixed infections in the two combinations of TMV-U1 + PepMoV and PMMoV+PepMoV. However, in cultivars of 'Manitta' and 'Bugang', synergistic symptoms were not noted, but mottling which was milder than that of single infection was produced. Cells infected singly with TMV-U1 and PMMoV in the cultivars of 'Cheongyang' and 'Wangshilgun', respectively, had the typical ultra-structures of tobamovirus as the stacked-band structure and multiple spiral aggregate (SA). In the cells and tissues infected with PepMoV on the cultivars of 'Cheongyang', 'Wangshilgun', 'Manitta' and 'Bugang', the potyvirus inclusions of pinwheels, scrolls, lamminated aggregates and amorphous inclusion were observed. In the cells infected mixedly with combinations of TMV­U1+PepMoV and PMMoV+PepMoV, the virus particles and inclusions of the two different viruses were found simultaneously in the same cytoplasm. The amounts of virus particles in mixed infections were more abundant than in single infection. The angled-layer aggregates (ALA) were observed only in the cells infected with both TMV-U1 and PepMoV.