• 한국환경독성학회:학술대회논문집
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• 한국환경독성학회 2003년도 춘계학술대회
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• pp.183-184
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• 2003

Sophoricoside was isolated as the inhibitor of IL-5 bioactivity from Sophora japonica (Leguminosae). It has been reported to have an anti-inflammatory effect on rat paw edema model. To develop as an anti-allergic drug, genotoxicity of sophoricoside was investigated in bacterial and mammalian cell system such as Ames bacterial test, chromosomal aberration assay, Comet assay and MOLY assay. In Ames test, sophoricoside of 5000 ∼ 313 $\mu\textrm{g}$/plate concentrations was not shown significant mutagenic effect in Salmonella typhimurium TA98, TA100, TA1535 and TA1537 strains. The cytotoxicity (IC$\_$50/ and IC$\_$20/) of sophoricoside was determined above the concentration of 5000 $\mu\textrm{g}$/ml in Chinese hamster lung (CHL) fibroblast cell and L5178Y mouse lymphoma cell line. At concentrations of 5000, 2500 and 1250 $\mu\textrm{g}$/ml, this compound was not induced chromosomal aberration in CHL fibroblast cell in the absence and presence of S-9 metabolic activation system. Also in comet assay, DNA damage was not observed in L5178Y cell line. Also in MOLY assay, sophoricoside of 5000 ∼ 313 $\mu\textrm{g}$/ml concentrations was not shown significant mutagenic effect in absence of S-9 metabolic activation system. However, the higher concentration of 5000 and 2500 $\mu\textrm{g}$/ml of sophoricoside induced the increased mutation frequency (MF) in the presence of S-9 metabolic activation system. From these results, no genotoxic effects of sophoricoside observed in bacterial systems whereas, genotoxic effects observed in mammalian cell systems in the presence of metabolic activation system. These results suggested that the metabolite(s) of sophoricoside can cause some genotoxic effects in mammalian cells.

• 대한한방종양학회지
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• 제6권1호
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• pp.47-65
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• 2000

In order to investigate antitumor and immune response effect by Hyangsapyungwisan after Sarcoma-180 cells and methotrexate were treatred each other, the extract of Hyangsapyungwisan was orally administered to ICR mice for 14 days. To evaluate the effects of the Hyangsapyungwisan, 50% inhibition concentration($IC_{50}$), mean survival days, tumor weight for antitumor effects, hemagglutinin titer, hemolysin titer, rosette forming cells, natural killer cell activity and productivity of interleukin-2 for immune responses measured in ICR mice. The results were summarized as follows: 1. Mean survival time in Hyangsapyungwisan-treated group was slightly prolonged, as compared with control group(13.46%). 2. On the MTT assay, cell viability was significantly inhibited by $5{\mu}g/well,\;2.5{\mu}g/well,\;1.25{\mu}g/well,\;and\;0.625{\mu}g/well$ of Hyangsapyung-wisan concentration inhibited cell viability significantly. $IC_{50}$ for cell viability was $11.59{\mu}g/well$. 3. Tumor weight in Hyangsapyungwisan treated group was depressed, as compared with the control group(p<0.05). 4. Hemagglutinin titer in Hyangsapyungwisan-treated group was slightly increased with no significance, as compared with the control group. 5. Hemolysin titer in Hyangsapyungwisan-treated group was silightly increased, as compared with the control group(p<0.05). 6. Rosette forming cells in Hyangsapyungwisan-treated group was silightly increased, as compared with the control group(p<0.05). 7. Naural killer cell activity in Hyangsapyungwisan-treated group was significantly increased(p<0.05). 8. Production of interleukin-2 was significantly increased(p<0.05). According to the above results, Hyangsapygwisan had prominent antitumor effects, and enhance both cellular and humoral immunity in mice.

• 대한본초학회지
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• 제26권1호
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• pp.133-138
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• 2011

Objectives : The purpose of this study was to estimate the antioxidant activity of Sesami Semen Nigrum extract (SSN) on mouse Leydig cells, TM3. Methods : Cell viability assays were performed. The protective effects of SSN against hydrogen peroxide-induced oxidative stress in Leydig cells were examined by measuring cell viability. Lipid peroxidation levels and antioxidant enzyme concentrations such as SOD and catalase were measured. Results : Cell viability of Leydig cells increased with SSN concentration. Cell viability of Leydig cells was 136.66% when SSN concentration was $50{\mu}g/ml$. Cell viability of the hydrogen peroxide group was statistically decreased (p<0.01) compared with the control group. Antioxidant effect of SSN was measured and the protective effect of SSN concentration were 5, 10, $50{\mu}g/ml$. LPO were decreased significantly at 5, $50{\mu}g/ml$ of SSN concentrations. SOD activity was increased at 1, 10, $50{\mu}g/ml$ of SSN concentrations. Catalase activity was significantly increased at 123.7, 133.3 and 131.9 units/mg protein when SSN concentrations were 5, 10 and $50{\mu}g/ml$, respectively. Conclusions : In conclusion, Sesami Semen Nigrum extract has antioxidant activities in Leydig cells against oxidative stress.

• Journal of Acupuncture Research
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• 제21권3호
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• pp.107-119
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• 2004

Objective : The purpose of this study was to investigate the effect of Bee Venom and melittin on the lipopolysaccharide(LPS) and sodium nitroprusside(SNP)-induced expressions of Cell viability, nitric oxide(NO), inducible nitric oxide synthase(iNOS), extra-signal response kinase(ERK), jun N-terminal Kinase(JNK) and p38 kinase(p38)- mitogen activated protein kinase(MAPK) Family- in RAW 264.7 cells, a murine macrophage cell line. Methods : The expressions of cell viability by MTT assay, NO by Nitrite assay and iNOS, ERK, JNK and p38 were determined by Western blotting. Results : 1. Compared with the control group, 0.5, 1, $5{\mu}g/m{\ell}$ bee venom and 5, $10{\mu}g/m{\ell}$ melittin increased cell viability of RAW 264.7 induced by LPS and SNP significantly respectively. 2. Compared with the control group, 0.5, 1, $5{\mu}g/m{\ell}$ bee venom and 5, $10{\mu}g/m{\ell}$ melittin inhibited expression of NO induced by LPS and SNP significantly respectively. 3. Compared with the control group, 1, $5{\mu}g/m{\ell}$ bee venom and 5, $10{\mu}g/m{\ell}$ melittin inhibited expression of iNOS induced by LPS significantly and 0.5, 1, $5{\mu}g/m{\ell}$ bee venom and 5, $10{\mu}g/m{\ell}$ melittin inhibited expression of iNOS induced by SNP significantly. 4. Compared with the control group, the expression of ERK induced by LPS and SNP decreased significantly in the treatment groups of $5{\mu}g/m{\ell}$ bee venom and 5, $10{\mu}g/m{\ell}$ melittin, which of p-ERK by LPS also did in 1, $5{\mu}g/m{\ell}$ bee venom and 5, $10{\mu}g/m{\ell}$ melittin, but which of p-ERK by SNP did not decrease. 5. Compared with the control group, the. expression of JNK induced by LPS and SNP decreased significantly in the treatment groups of 5, $10{\mu}g/m{\ell}$ melittin, which of p-JNK by LPS in 5, $10{\mu}g/m{\ell}$ melittin and by SNP in $1{\mu}g/m{\ell}$ bee venom and $10{\mu}g/m{\ell}$ melittin decreased significantly. 6. Compared with the control group, the expression of p38 induced by LPS did not have significant difference, which induced by SNP decreased significantly in the treatment groups of 1, $5{\mu}g/m{\ell}$ bee venom and 5, $10{\mu}g/m{\ell}$ melittin. p-p38 induced by LPS decreased significantly in the treatment group of $10{\mu}g/m{\ell}$ of melittin, which induced by SNP also decreased significantly in 0.5, 1, $5{\mu}g/m{\ell}$ bee venom and 5, $10{\mu}g/m{\ell}$ melittin.

• 한국작물학회지
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• 제60권2호
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• pp.239-247
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• 2015

This experiment was conducted to obtain the have higher contents of pharmaceutical constituents as well as higher yield from colchicine induced diploid and tetraploid extracts of Platycodon grandiflorum. In order to determine the biological activity, this study was focused to evaluate the cytotoxicity, antimicrobial on the bronthus disease bacteria, antioxidant enzyme activity of diploid and tetraploid extracts in P. grandiflorum. The activities of antioxidant enzyme according to different solvent extracts were measured as superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX). The cytotoxicity of methanol extracts of P. grandiflorum showed significant differences between tetraploid and diploid. That is, the cytotoxic effect against human cancer cell was higher in tetraploid than in diploid. At all extracts concentration, tetraploid samples showed high toxicity and the $IC_{50}$ (concentration causing 50% cell death) value showed the highest on HCT-116 cell ($105.91{\mu}g/mL$), and exhibited significant activity against the Hep 3B cell ($140.67{\mu}g/mL$), SNU-1066 cell ($154.01{\mu}g/mL$), Hela cell ($158.37{\mu}g/mL$), SNU-601 cell ($182.67{\mu}g/mL$), Calu-6 cell ($190.42{\mu}g/mL$), MCF-7 cell ($510.19{\mu}g/mL$). Antimicrobial activities of diploid P. grandiflorum were relatively low compared to tetraploid P. grandiflorum on most of the bacterial strains. In tetraploid P. grandiflorum, K. pneumoniae showed the clear zone formation (18~19 mm) of growth inhibition, followed by the clear zone formation of 13~15 mm on C. diphtheria and S. pyogenes. The antimicrobial activities in diploid P. grandiflorum were the highest on K. pneumonia (14~15 mm), and showed the clear zone formation of 11~12 mm on C. diphtheria and 12~13 mm on S. pyogenes. The antimicrobial activity is thought to look different depending on the bacterial strains and the polyploidy of P. grandiflorum. The root extract of P. grandiflorum had the highest (97.2%) SOD enzyme activity in ethyl acetate partition layer of tetraploid while water partition layer of diploid showed the lowest (48.6%) SOD enzyme activity. The activity of CAT showed higher values in the root of tetraploid than in the diploid of P. grandiflorum in all partition layers except butyl alcohol. The activities of APX and POD showed higher values in the root of tetraploid than in the diploid of P. grandiflorum in all fraction solvents except water layer. These results indicate that the tetraploid P. grandiflorum can be used as a source for developing cytotoxic agent and antimicrobials which can act against bronchus diseases bacterial strains.

• 식물조직배양학회지
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• 제24권6호
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• pp.361-367
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• 1997

담배(Nicotiana tabacum cv. BY4)의 이배체식물과 담배의 약으로부터 유도된 반수체식물의 잎절편을 사용하여 2.4-D를 단독톄처리하여 캘러스를 유도하였다. 캘러스증식은 이배체에서는 2.0 mg/L 2.4-D와 1.0mg/L 카이네틴의 조합처리구에서, 반수체의 경우에 있어서는 2.0mg/L 2.4-D와 0.1mg/L BAP 조합처리구에서 실시하였으며, 현탁배양도 동일조건으로 실시하였다. 현탁배양 세포괴를 배양 15-20일째가 되면 0.8% low melting agarose와 혼합하여 카드a뮴이 0-2,000 $\mu$M의 농도로 처리된 선발배지에 평판배양하여 카드뮴저항성 colony(cell clump)를 조사관찰하였다. 그 결과 본 실험에서는 이배체는 세포의 성장에 있어서 최하저해농도(MIC : Minimum Inhibition Concentration)는 Cd$^{++}$ 300 $\mu$M이었으며 반수체는 Cd$^{++}$ 200 $\mu$M이었다. 이때 반수체 및 이배체의 MIC에서 선발된 세포주를 카드뮴 500, 1,000, 2,000 $\mu$M 농도로 옳겨 경화시킨 후 카드뮴저항성을 선발하였고 그 특성을 조사하였다. 그 결과 카드뮴 저항성을 띤 반수체 및 이배체유래 캘러스의 경우에는 각각 카드a뮴의 농도가 증가할수록 생중량은 감소하였으나, 건조중량은 오히려 증가하였다. 그리고 각각 선발된 세포주내의 카드뮴의 함량을 AAS를 사용하여 측정하였던 바, 카드뮴의 농도가 높을수록 캘러스에 축적된 카드뮴의 양이 높게 나타났다.

• Applied Microscopy
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• 제24권3호
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• pp.34-45
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• 1994

This investigation has been carried out to examine the structure of digestive tract from Korean Leech, Erpobdella lineata, using light and electron microscope. The digestive tract is composed of mouth, pharynx, Oesophagus, six-chambered stomach, three-chambered intestine, rectum and anus. Stomach and intestine have not gastric or intestine ceca and consist of only straight tube. All digestive tracts from pharynx to rectum are covered with simple columnar epithelial cells. While the surfaces of endothelial cell of pharynx and rectum are covered with cuticular layer of about $0.3{\mu}m$ in thickness, stomach and intestine are covered with estimated $0.2-0.3{\mu}m$ and $0.5{\mu}m$ microvilli respectively. Circular folds were found only in first and second chambers of stomach, intestine and rectum, but not in pharynx and the other chambers (third to sixth) of stomach. The granules of $0.3-0.8{\mu}m$ and $0.5-1.0{\mu}m$ in diameter were observed in the cytoplasm of stomach endothelial cell. These granules were demonstrated to contain protein which showed a positive reaction to ninhydrin. It was also found that there are well-developed microvilli in the apical portion of intestine endothelial cell in which endocytosis occurs actively.

• 생태와환경
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• 제50권1호
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• pp.16-28
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• 2017

In an aquatic environment, toxicity of metals to organisms depends on external factors (type of metal, exposure concentration and duration, environmental parameters, and water quality) and intracellular processes(metal-binding sites and detoxification). Toxicity of copper(Cu) on the marine microalga Tetraselmis suecica was investigated in this study. Dose-dependent (Cu concentration dependent) inhibition of growth and cell division, as well as, variation of intra- and extra-cellular Cu, Fe and Zn content was observed. T. suecica was sensitive to Cu; the 96 h $EC_{50}$ (concentration to inhibit growth-rate by 50%) of growth rate (${\mu}$) ($21.73{\mu}M\;L^{-1}$), cell division $day^{-1}$ ($18.39{\mu}M\;L^{-1}$), and cells $mL^{-1}$ ($13.25{\mu}M\;L^{-1}$) demonstrate the toxicity of Cu on this microalga. High intra-($19.86Pg\;cell^{-1}$) and extra-cellular($54.73Pg\;cell^{-1}$) Cu concentrations were recorded, on exposure to 24.3 and $72.9{\mu}M\;L^{-1}$ of Cu.

• 한국동물학회지
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• 제30권1호
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• pp.53-70
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• 1987

실험에 사용한 플라나리아는 Dugesia japonica Ichikawa et Kawakatsu로서 핵형분석에 의하여 종을 확인하였다, 이 종의 유조직을 구성하는 세포들의 세포화학적 및 미세구조적 특징을 밝히고자 본 실험을 행하였다. 세포화학적으로 방법으로는 hematoxyline-edsin, periodie, acid-schiff(PAS),PAS-alcian blue 반응 그리고 methylene blue-basic fuchisn 두으로 염색으 시행하였고, 유조직의 미세구조를 투과전자현미경으로 관찰하여 다음과 같은 결과를 얻었다. 유조직에는 두 조의 유조직 세포 이외에 봉상체형성세포와 종의 호염기성세포, 1종의 호산성세포 그리고 투명과립세포 7종의 과립세포가 관찰되었다. 1.유리유조직세포(Free parenchymal cell): 핵막 바로 주위에 세포질에는 다수의 chromatoid body가 존재하였고 세포질에 비하여 핵은 매우 컸다. 핵질에서는 뚜렷한 인을 관찰할 수 있었고 큰 염색질은 핵질에 균일하게 많이 분포되었다. 2.고정유세포( Fixed parenchymal cell): 이 세포는 불규칙한 모습으로 많은 세포돌기를 내고 있었고, 세포질에는 전자밀도가 매우 낮은 투명질에 과립성소포체, mitochondria 그리고 Golgi체등이 발달되어 었었다. 3.봉상체 형성세포(Rhabdite-forming cell): 세포질에는 전자밀도가 매우 높은 봉상체과립(0.3 x 0.9 $\mu$m)들을 포함하였으며 과립성소포체,mitochondria 그리고 Golgi체 등의 세포소기관도 매우 발달되어 있었다. 4. A형 호염기성과립세포(Basophilic granule cell type A): 세포질내에는 alcian blue에 강한 양성반응을 나타내고 전자밀도가 매우 높은 불규칙한 형태의 과립 (1.4 x 0.7 $\mu$m)들이 밀접되어 있었다. 5. C형 호기성과립세포(Basophilic granule cell type C): 세포질에는 PAS반응에 양성을 나타내는 작은 과립(0.2$\mu$m)들이 분산되어 있었다. 이 유형의 과립들은 근육층에서도 관찰되었다. 6. D형 호기성과립세포(Basophilic granule cell type D): 생긱기관 주위의 유조직에서만 관찰되었으며 세포질에는 PAS반응에 강한 양성을 보이고 또한 약한 eosinophilic도 나타내는 원형의 과립들이 대부분 집단을 이루웠다. 7. E형 호기성과립세포(Basophilic granule cell type E): 생식기관 주위의 유조직내에서만 관찰되었으며, 세포질내에는 PAS양성과립(약0.2$\mu$m)이 극소수 분산되어 있었다. 8. 호산성 과립세포(Eosinophilic granule cell): 세포질에는 eosinophilia를 나타내며, 전자밀도가 매우 높은 구형 또는 불규칙형과립(0.3$\times$0.2-8$\times$0.4$\mu$m)들이 존재하였으며, cisternae가 넓은 과립성소포체도 매우 발달되어 있었다. 9. 투명과립세포(Transparent granule cell): 본 실험에서 실시된 세포화학적 염색반응에서 음성을 나타내는 투명과립을 포함하고 있는 이같은 세포들은 D형 호염기성과립세포 주위에서 주로 관찰되었다.

• 식물조직배양학회지
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• 제28권6호
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• pp.311-315
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• 2001

담배 (Nicotiana tabacum L cv. Xanthi) 및 애기장대 (Arabidopsis thaliana cv. Col-0)에서 다양한 발달단계의 잎으로부터 원형질체를 분리하여 세포 크기와 엽록체의 크기 및 수의 관계를 조사하였다. 담배 원형질체의 지름이 15.6 $\mu\textrm{m}$ 이하인 작은 세포는 20여 개 이하의 엽록체를 가지고 있었는데, 엽록체의 평균 크기는 두께 0.93 $\mu\textrm{m}$, 길이 3.3$\mu\textrm{m}$로 나타났다. 잎이 신장되어 원형질체의 지름이 평균 30 $\mu\textrm{m}$에서 45 $\mu\textrm{m}$로 증가함에 따라, 엽록체는 두께 1.57 $\mu\textrm{m}$, 길이 5.55 $\mu\textrm{m}$의 크기로 일정하게 유지되었으나 엽록체의 수는 평균 42개에서 101개로 증가하였다. 이와 같이 담배에서 잎의 크기 또는 세포의 크기와 엽록체의 수 또는 부피는 비례관계를 나타냈고 동일한 경향이 애기장대에서도 관찰되었다. 세포 부피에 대한 총 엽록체의 부피의 비는 다양한 발달단계에서 담배는 10.5%, 애기장대는 32.5%로 일정하게 유지되었다.