• 한국동물학회:학술대회논문집
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• 한국동물학회 1997년도 한국생물과학협회 학술발표대회
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• pp.196.1-196
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• 1997

No Abstract, See Full Text

• 한국동물학회:학술대회논문집
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• 한국동물학회 1999년도 한국생물과학협회 학술발표대회
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• pp.176.2-176
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• 1999

No Abstract, See Full Text

• BMB Reports
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• 제42권2호
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• pp.72-80
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• 2009

In mammals, major progress has recently been made with the dissection of early embryonic cell specification, the isolation of stem cells from early embryos, and the production of embryonic-like stem cells from adult cells. These studies have overcome long-standing species barriers for stem cell isolation, have revealed a deeper than expected similarity of embryo cell types across species, and have led to a better understanding of the lineage identities of embryo-derived stem cells, most notably of mouse and human embryonic stem (ES) cells. Thus, it has now become possible to propose a species-overarching classification of embryo stem cells, which are defined here as pre- to early post-implantation conceptus-derived stem cell types that maintain embryonic lineage identities in vitro. The present article gives an overview of these cells and discusses their relationships with each other and the conceptus. Consequently, it is debated whether further embryo stem cell types await isolation, and the study of the earliest extraembryonically committed stem cells is identified as a promising new research field.

• 한국가축번식학회지
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• 제10권1호
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• pp.42-48
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• 1986

These experiments were carried out to develop new techniques identifying XX-bearing embryos prior to implantation by immunological method. Antiserum to histocompatibility-Y(H-Y) antigen was prepared in adult SD(sprague-dawley) female rat by repeated immunization of newbone testis supernatant from males of the same strain. ELISA test was used to identify the H-Y antibody of antiserum. Total 124 mouse embryos (8-cell stage) were treated with H-Y antiserum and complement in BSA free Ho, pp. and Pitt's medium and cultured under the gas phase of 5% CO2 in air at 37$^{\circ}C$ for 24 to 48 hrs. The morphological characteristics of embryos treated were observed under the phase-contrast micro scope. The results obtained in these experiments were summarized as follows: 1. Optimal Density of H-Y antibody were a, pp.ared to be 0.27-0.47 by ELISA test. 2. Of total 124 embryos treated with H-Y antiserum and complement 69(55.6%) embryos developed to blastocyst and 55(44.4%) destroyed or arrested.

• Animal cells and systems
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• 제10권1호
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• pp.41-47
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• 2006

Development of the mammalian pre-implantation embryos has unique features, such as a slow and unsynchronized cell division, compaction, and eventual formation of blastocysts with inner cell mass and trophectoderm. In order to have a clue on molecular mechanisms that reside in mouse early development, we suppressed expression of early embryo-specific genes with RNAi and observed their development in vitro. We observed developmental defects in embryos microinjected with dsRNAs for Oct4 or Nanog among the tested genes. Careful examinations revealed that development of the most of the Oct4- or Nanog-suppressed embryos were arrested at the morula stage. These results suggest that the Oct4 and Nanog activities are also required for embryogenesis earlier than the blastocyst stage.

• 약학회지
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• 제58권3호
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• pp.151-157
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• 2014

Water extracts of rice grasshopper (Oxya japonica japonica Thurnberg) were prepared and their antitumor and immunostimulatory activities were investigated using a flow cytometer. When LCT-CT was ip injected into ICR mice at the dose of 33.3 mg/kg before and after the implantation of $4{\times}10^5$ cells/mouse of sarcoma 180 tumor cells, it inhibited the growth of the tumor cells by 96.6%, showed lymphoblstogenic activities on the splenic lymphocytes and increased the expression of CD25 molecules on the splenic T lymphocytes. When co-cultured with the splenic lymphocytes of a BALB/c mouse, LCT-CT showed strong immunostimulatory activities at the concentration of $25{\sim}100{\mu}g/ml$ by significantly increasing lymphoblasts ratio and CD25 expression.

• 한국가축번식학회지
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• 제10권2호
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• pp.175-181
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• 1986

These experiments were carried out to clarify the effects various kinds of cryoprotectants which were frequently used in freezing embryos of domestic animals on the survival of frozen-thawed mouse embryos. As cryoprotectant, glycerol, DMSO and methanol were used and the procedures of adding them in medium were practiced by one-step or six-step adding method. Morphologically normal mouse embryos developed to blastocyst by in vitro culture after freezing and thawing were transferred to pseudopregnant recipients by surgical procedures. The results obtained in these experiments were summarized as follows: 1. The survival rates of the frozen-thawed 8-cell embryos, morulas and blastocysts following one-step addition of glycerol were 83.6, 80.3 adn 70.3%, respectively, while following six-step addition of glycerol, 69.2, 56.3 and 66.7% respectively. 2. When glycerol, DMSO and methanol were used as cryoprotectant under the same condition of freezing and thawing, the survival rates of frozen-thawed embryos were 74.0, 76.1 and 37.6%, respectively. 3. The implantation rate of embryos transferred to pseudopregnant recipients after freezing and thawing was 49.2%.

• The Journal of Advanced Prosthodontics
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• 제6권3호
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• pp.157-164
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• 2014

PURPOSE. This study focused on in vitro cell differentiation and surface characteristics in a magnesium coated titanium surface implanted on using a plasma ion source. MATERIALS AND METHODS. 40 commercially made pure titanium discs were prepared to produce Ti oxide machined surface (M) and Mg-incorporated Ti oxide machined surface (MM). Surface properties were analyzed using a scanning electron microscopy (SEM). On each surface, alkaline phosphatase (ALP) activity, alizarin red S staining for mineralization of MC3T3-E1 cells, and quantitative analysis of osteoblastic gene expression, were evaluated. Actin ring formation assay and gene expression analysis of TRAP and GAPDH performing RT-PCR were performed to characterize osteoclast differentiation on mouse bone marrow-derived macrophages (BMMs). RESULTS. MM showed similar surface morphology and surface roughness with M, but was slightly smoother after ion implantation at the micron scale. M was more hydrophobic than MM. No significant difference between surfaces on ALP activity at 7 and 14 days were observed. Real-time PCR analyses showed similar levels of mRNA expression of the osteoblast phenotype genes; osteopontin (OPN), osteocalcin (OCN), bone sialoprotein (BSP), and collagen 1 (Col 1) in cell grown on MM at 7, 14 and 21 days. Alizarin red S staining at 21 days showed no significant difference. BMMs differentiation increased in M and MM. Actin ring formation assay and gene expression analysis of TRAP showed osteoclast differentiation to be more active on MM. CONCLUSION. Both M and MM have a good effect on osteoblastic cell differentiation, but MM may speed the bone remodeling process by activating on osteoclast differentiation.

• 융합정보논문지
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• 제8권2호
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• pp.61-65
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• 2018

본 연구는 이식된 줄기세포들이 혈관용 클립압박으로 유도된 척수경색 동물들에서 행동학적 결핍을 감소시키는 연구를 진행하였다. 흉수신경 9번과 10번에 척수 손상후 5일후에 배아줄기세포 이식을 통해서 배아줄기세포가 경색부위를 채워지게 되므로 이식후 손상부위의 조직학적 감소와 신경세포군의 조직학적 재생을 증명하는데 중점을 두었다. 본 연구를 통해 마우스 배아줄기세포의 이식이 중증 척수 손상후 행동학적 발달을 보여주는 명백한 결과들을 도출하였음을 보여주고 있다. 이러한 마우스 배아줄기세포는 신경학적 손상에 대한 치료로서 사용될 수 있는 처치법이다. 결론적으로, 줄기세포 적용은 손상조직을 재생시켜서 기능적, 행동적 향상에 기여할 수 있기에 다양한 줄기세포 치료법을 통해 임상적 적용을 위한 중요한 치료법이 될 수 있다.

• 한국가축번식학회지
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• 제22권2호
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• pp.171-176
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• 1998

본 연구는 체외에서 배양된 생쥐 완전탈출 배반포기배를 동결보존액 EFS35를 이용하여 초자화 동결하였을 때 체내발달의 적합성 여부를 조사하기 위해 실시하였다. 공시된 완전탈출 배반포기배($\theta$ 130$\mu\textrm{m}$)는 체내에서 생산된 전핵기 수정란을 5일 동안 체외배양하여 얻었으며, 10% ethylene glycol(EG)에 5분 노출한 후 EFS35(35% EG, 18% Ficoll, 0.3 M sucrose)에 30초 동안 노출하거나, 초자화 동결하였다. 융해 후, 재팽창이 이루어진 완전탈출 배반포기배는 가임신 3일된 대리모의 한쪽 또는 양쪽 자궁각(4~6개/자궁각)에 이식하였다. 대리모의 임신율과 착상율은 임신 15일재 외과적 해부로 판정하였다. 그 결과를 요약하면 다음과 같다. 융해 30분 후, 완전탈출 배반포기배의 체외생존율은 노출군(65.5%)과 동결군(54.5%)간에 유의한 차이는 없었다. 또한, 체내발달율을 조사하였던 바, 착상율에 있어서 동결군(41.0%)과 대조군(58.5%)간에 유의한 차는 없었지만, 정상산자율에서는 동결군(24.0%)의 결과가 대조군(58.3%)보다 유의하게 낮게 나타났다(p<0.05). 이러한 결과는 EFS35를 이용한 완전탈출 배반포기배의 초자화 동결은 정상산자율은 감소하였지만, 완전탈출 배반포기배의 이용 효율성을 넓히는데 이용될 수 있다는 것을 알 수 있었다.