• Title/Summary/Keyword: Mosaic patterns

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Environmental Risk Assessment of Watermelon Grafted onto Transgenic Rootstock Resistant to Cucumber Green Mottle Mosaic Virus (CGMMV) on Non-Target Insects in Conventional Agro-Ecosystem

  • Yi, Hoon-Bok;Park, Ji-Eun;Kwon, Min-Chul;Park, Sang-Kyu;Kim, Chang-Gi;Jeong, Soon-Chun;Yoon, Won-Kee;Park, Sang-Mi;Han, Sang-Lyul;Harn, Chee-Hark;Kim, Hwan-Mook
    • Journal of Ecology and Environment
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    • v.29 no.4
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    • pp.323-330
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    • 2006
  • We investigated the impact of watermelon grafted onto Cucumber Green Mottle Mosaic Virus (CGMMV)-resistant transgenic watermelon rootstock on insects as non-target organisms in a greenhouse in 2005. We quantitatively collected insect assemblages living on leaves and flowers, and we used sticky traps to collect alate insects. We compared the patterns of insect assemblages and community composition, cotton aphid (Aphis gossypii Glover) on watermelon leaves and western flower thrip (Frankliniella occidentalis Trybom) on watermelon male flowers, between CGMMV-resistant transgenic watermelon (TR) and non-transgenic watermelon (nTR). Non-parametric multidimensional scaling (NMS) ordination verified that insect assemblages on leaves and sticky traps were different between TR and nTR (P<0.05). The insect assemblages on male flowers were not statistically significant. Multi-response permutation procedures proofed our results from NMS results (P>0.05). Conclusively, TR watermelons appear to have some adverse effects on the population of cotton aphids on leaves and sticky traps, but watermelon male flowers do not show an adverse effect. Further research is required to assess the effect of TR on the aphid and western flower thrip. Life table experiments might support the specific reason for the adverse effects from leaf assemblages. Assessment of non-target impacts is an essential part of the risk assessment of non-target insects for the impact of transgenic organisms.

Studies on the Processing Properties and Interactions Between Porcine Blood Proteins and Waxy Rice Starch During Making Porcine Blood Cake

  • Lin, Chin-Wen;Yang, Jeng-Huh;Chu, Hsien-Pin;Su, Ho-Ping;Chen, Hsiao-Ling;Huang, Chia-Cheong
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.3
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    • pp.358-364
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    • 2001
  • The physiochemical properties and interactions between porcine blood and waxy rice were determined. Addition of calcium chloride (0.15%) improved acceptability of blood cake and increased the gelatinization degree of waxy rice. The water-holding capacity of porcine blood gel (blood/water=60/40, v/v), extent of absorption and gelatinization of waxy rice, and scanning electron microscopy showed that blood protein matrix and waxy rice are competitors for holding water in the cooking procedure. Non-haem iron content increased linearly (R=0.95) when heating temperature rose. The presence of blood proteins caused increasing of peak temperature (Tp) of gelatinization in differential scanning calorimetric thermal gram, The microstnlcture of plasma proteins and haemoglobin appeared continuous changes, and interacted with surface of waxy rice flour in terms of network and mosaic form, respectively. The electrophoretic patterns revealed an interaction between plasma proteins and waxy rice glutelin and haemoglobin when heated could be found at temperatures above $60^{\circ}C$.

Spatial Configuration of Stars Around Three Metal-poor Globular Clusters in the Galatic Bulge, NGC 6266, NGC 6273, and NGC 6681 : Surface Density Map and Radial Density Profile

  • Han, Mihwa;Chun, Sang-Hyun;Choudhury, Samyaday;Chiang, Howoo;Lee, Sowon;Sohn, Young-Jong
    • Journal of Astronomy and Space Sciences
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    • v.34 no.2
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    • pp.83-97
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    • 2017
  • We present extra-tidal features of spatial configuration of stars around three metal-poor globular clusters (NGC 6266, NGC 6273, NGC 6681) located in the Galactic bulge. The wide-field photometric data were obtained in BVI bands with the MOSAIC II camera at CTIO 4 m Blanco telescope. The derived color-magnitude diagrams (CMDs) contain stars in a total $71^{\prime}{\times}71^{\prime}$ area including a cluster and its surrounding field outside of the tidal radius of the cluster. Applying statistical filtering technique, we minimized the field star contaminations on the obtained cluster CMDs and extracted the cluster members. On the spatial stellar density maps around the target clusters, we found overdensity features beyond the tidal radii of the clusters. We also found that the radial density profiles of the clusters show departures from the best-fit King model for their outer regions which support the overdensity patterns.

AltMV TGB1 Nucleolar Localization Requires Homologous Interaction and Correlates with Cell Wall Localization Associated with Cell-to-Cell Movement

  • Nam, Jiryun;Nam, Moon;Bae, Hanhong;Lee, Cheolho;Lee, Bong-Chun;Hammond, John;Lim, Hyoun-Sub
    • The Plant Pathology Journal
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    • v.29 no.4
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    • pp.454-459
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    • 2013
  • The Potexvirus Alternanthera mosaic virus (AltMV) has multifunctional triple gene block (TGB) proteins, among which our studies have focused on the properties of the TGB1 protein. The TGB1 of AltMV has functions including RNA binding, RNA silencing suppression, and cell-to-cell movement, and is known to form homologous interactions. The helicase domains of AltMV TGB1 were separately mutated to identify which regions are involved in homologous TGB1 interactions. The yeast two hybrid system and Bimolecular Fluorescence Complementation (BiFC) in planta were utilized to examine homologous interactions of the mutants. Helicase motif I of AltMV TGB1 was found to be critical to maintain homologous interactions. Mutations in the remaining helicase motifs did not inhibit TGB1 homologous interactions. In the absence of homologous interaction of TGB1, subcellular localization of helicase domain I mutants showed distinctively different patterns from that of WT TGB1. These results provide important information to study viral movement and replication of AltMV.

Bird Communities in Rice Field and Grasslands during the Dry Season in the Mekong Delta, Cambodia

  • Kry, Msphal;Lee, Woo-Shin;Park, Sung Jin;Rhim, Shin-Jae
    • Journal of Korean Society of Forest Science
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    • v.98 no.2
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    • pp.197-202
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    • 2009
  • We studied bird communities among in rice field (46 plots), short grallland (47plot), and tall grass mixed shrub (21 plots) during dry season, January 2007 in Boeung Prek Lapouv (BPL) Important Bird Area, Mekong Delta, Cambodia using the point-count method. Water depth, vegetation height and shrub density were significantly different among the three study areas. We recorded, 60 bird species, of which 13 differed significantly among habitats. Landbirds such as Grey-breasted Prinia (Prinia hodgsonii) and Oliver-black Sunbird (Nectarinia jugularis) were much more abundant (P<0.0001) in the tall grass mixed shrub, whereas the Sarus Crane (Grus antigone), herons and egrets were more abundant in the short grass (P<0.05). Bird species richness and species abundances were not significantly different among habitats. Bird species diversity (H') was higher in grasslands than in rice fields. Mosaic habitat matrix, such as rice field, short grass, and tall grass mixed shrub, should be maintained for the extend and quality of wetlands, and the prevailing human-land use patterns, appear adequate and conductive for waterbirds in the Mekong Delta, Cambodia.

GUS Expression by CaMV 35S and Rice Act1 Promoters in Transgenic Rice

  • Kwang-Woong Lee
    • Journal of Plant Biology
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    • v.37 no.3
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    • pp.371-380
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    • 1994
  • To determine the patterns and the levels of expression of the cauliflower mosaic virus (CaMV 35S) promoter and the rice actin 1 (Act1) promoter in rice, transgenic rice plants containing CaMV 35S-$\beta$-glucuronidase (GUS) and Act1-GUS constructs were generated and examined by fluorometric and histochemical analyses. The fluorometric analysis of stably transformed calluses showed that the activity of the rice Act1 promoter was stronger than that of the CaMV 35S promoter in rice cells. In a histochemcial study of the transgenic rices, it was shown that the GUS activity directed by the CaMV 35S promoter was localized mainly in parenchymal cells of vascular tissues of leaves and roots and mesophyll cells of leaves. These results are similar to those of potato, a dicot plant. In contrast, rice plant transformed with Act1-GUS fusion construct revealed strong GUS activity in parenchymal cells of vascular tissue, mesophyll cells, epidermal cells, bulliform cells, guard subsidiary cells of leaves and most cells of the root, suggesting that the rice Act1 promoter is more constitutive than the CaMV 35S promoter. It was also confirmed that in both types of transgenic rice little or no staining was localized in metaxylen tracheary elements of vascular tissue from leaves or roots. These results indicate that the rice Act1 promoter can be utilized more successfully for expression of a variety of foreign gene in rice than the CaMV 35S promoter.

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Microhabitat Characteristics Determine Fish Community Structure in a Small Stream (Yudeung Stream, South Korea)

  • Choi, Jong-Yun;Kim, Seong-Ki;Kim, Jeong-Cheol;Lee, Hyeon-Jeong;Kwon, Hyo-Jeong;Yun, Jong-Hak
    • Proceedings of the National Institute of Ecology of the Republic of Korea
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    • v.2 no.1
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    • pp.53-61
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    • 2021
  • Distribution of fish community depends largely on environmental disturbance such as habitat change. In this study, we evaluated the impact of environmental variables and microhabitat patch types on fish distribution in Yudeung Stream at 15 sites between early May and late June 2019. We used non-metric multidimensional scaling to examine the distribution patterns of fish in each site. Gnathopogon strigatus, Squalidus gracilis majimae, Zacco koreanus, and Zacco platypus were associated with riffle and boulder areas, whereas Iksookimia koreensis, Acheilognathus koreensis, Coreoleuciscus splendidus, Sarcocheilichthys nigripinnis morii, and Odontobutis interrupta were associated with large shallow areas. In contrast, Cyprinus carpio, Carassius auratus, Lepomis macrochirus, and Micropterus salmoides were found at downstream sites associated with large pool areas, sandy/clay-bottomed areas, and vegetated areas. On the basis of these results, we suggest that microhabitat patch types are important in determining the diversity and abundance of fish communities, since a mosaic of different microhabitats supports diverse fish species. As such, microhabitat patches are key components of freshwater stream ecosystem heterogeneity, and a suitable patch composition in stream construction or restoration schemes will support ecologically healthy food webs.

First Report of Apricot pseudo-chlorotic leaf spot virus Infecting Peach Trees in South Korea (복숭아나무에서 검출된 Apricot pseudo-chlorotic leaf spot virus의 국내 첫 보고)

  • Bak, Sangmin;Seo, Euncheol;Kim, San Yeong;Park, Won Heum;Lee, Su-Heon
    • Research in Plant Disease
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    • v.23 no.1
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    • pp.75-81
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    • 2017
  • In May 2016, 24 peach samples showing abnormal and virus like symptoms were collected in one of major peach producing area, Yeongcheon-si, Gyeongsangbuk-do, Korea. We performed RT-PCR diagnosis for confirmation of viral infection. The diagnostic targets are 17 species of viruses and viroids that quarantine and high risk pathogens when it occur. As a results, seven species of viruses and viroids, including an unreported (Apricot pseudo-chlorotic leaf spot virus, APCLSV) and a quarantine (Peach latent mosaic viroid, PLMVd) species in Korea, were detected. For the sequence analysis of unreported virus, APCLSV, the sequence of coat protein gene were amplified and cloned. The sequence showed 97% nucleotide identity with other APCLSV isolates and compared with other seven species of reported Trichoviruses. This virus was classified as APCLSV based on the sequence and phylogenetic analysis. This isolate was named Yeongcheon. As patterns of APCLSV occurrence, all samples that APCLSV detected were co-infected with Apple chlorotic leaf spot virus (ACLSV). As properties of ACLSV, APCLSV has high possibility of wide spread disease in fruit tree farms in Korea. Therefore, it is necessary to do related researches, such as infection route and influence of disease in commercial orchards.

A case of bronchiolitis obliterans developed after adenovirus type 7 pneumonia (7형 아데노바이러스 폐렴 후 발생한 폐쇄 세기관지염 1례)

  • Park, Hyo-Khan;Lee, So-Yeon;Kim, Young-Ho;Oh, Phil-Soo;Kim, Jae-Yoon;Jung, Yoon-Seok;Kang, Chun;Kim, Kwang-Nam
    • Pediatric Infection and Vaccine
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    • v.14 no.1
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    • pp.124-128
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    • 2007
  • Bronchiolitis obliterans is a clinical syndrome of chronic obstruction associated with inflammatory changes in the small airways. There are marked variations in the epidemiology of this disease. In childhood, bronchiolitis obliterans has been described as a result of a number of infections such as adenovirus, measles, Bordetella pertussis, Mycoplasma pneumoniae, and influenza A infection. Most common agents are adenovirus types 3, 7, and 21. Diagnosis of bronchiolitis obliterans can be made based on clinical findings, high resolution computed tomography (HRCT) and lung biopsy. In addition to diagnosis, treatment is not yet clearly established. The authors experienced a case of bronchiolitis obliterans developed in 3 year-old girl who suffered from type 7 adenoviral pneumonia. She had been hospitalized and treated for 15 days due to pneumonia. After discharge, productive cough was not improved and auscultation revealed wheezing. HRCT demonstrated multifocal mosaic patterns suggesting bronchiolitis obliterans. She was managed with inhaled steroid and bronchodilator, and her symptoms were improved. However, follow up HRCT showed no interval change.

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Development of Stochastic Model and Simulation for Spatial Process Using Remotely Sensed Data : Fire Arrival Process (원격탐사자료를 이용한 공간적 현상의 모형화 및 시뮬레이션 : 자연화재발생의 경우)

  • 정명희
    • Spatial Information Research
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    • v.6 no.1
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    • pp.77-90
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    • 1998
  • The complex interactions of climate, topography, geology, biota and hwnan activities result in the land cover patterns, which are impacted by natural disturbances such as fire, earthquake and flood. Natural disturbances disrupt ecosystem communities and change the physical environment, thereby generating a new landscape. Community ecologists believe that disturbance is critical in determining how diverse ecological systems function. Fires were once a major agent of disturbance in the North American tall grass prairies, African savannas, and Australian bush. The major focus of this research was to develop stochastic model of spatial process of disturbance or spatial events and simulate the process based on the developed model and it was applied to the fire arrival process in the Great Victoria Desert of Australia, where wildfires generate a mosaic of patches of habitat at various stages of post-fire succession. For this research, Landsat Multi-Spectral Scanner(MSS) data covering the period from 1972 to 1994 were utilized. Fire arrival process is characterized as a spatial point pattern irregularly distributed within a region of space. Here, nonhomogeneous planar Poisson process is proposed as a model for the fire arrival process and rejection sampling thinning the homogeneous Poisson process is used for its simulation.

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