• Journal of Life Science
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• 제11권2호
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• pp.126-134
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• 2001

The phylogenetic tree displayed the presence of five groups in the Phellinus genus, which were distinguished based on their morphology. Most of the p. linteus appeared a cluster which was highly significant with the exception of P. linteus KACC 500122 and KACC 500411. They formed the sister taxa of P 1inteus where P. baumii, Phellinus sp. MPNU 7003, MPNU 7007, and MPNU 7010 had similar morphological characteristics. Also, P. nigricans IMSNU 32024 and P. pini var, carniformans IMSNU 32031 were grouped in the same cluster with P. igniarius KCTC 6227, KCTC 6228, and P. chrysoloma KCTC 6225 extracted from the Gen-Bank database. P. torulosus IMSNU 32028 and Phellinus sp. MPNU 7011 formed a closed group, however, these species had a distant taxa when compared with the other Phellinus species. The nucleotide sequences of the internal transcribed spacer (ITS) regions of ribosomal DNA (rDNA) including the 5.85 rDNA were determined from 24 strains of the Phellinus genus in order to analyze their phylogenetic relationship. These fungi were divided into two basic groups based on their ITS length, however, this grouping was different from that based on their morphological characteristics. Although various ITS sequences were ambiguously aligned, conserved sites were also identified. Accordingly, a neighbor-joining tree was constructed using the nucleotide sequence data of the conserved sites of the ITS regions and the 5.8S rDNA.

• 식물분류학회지
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• 제34권3호
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• pp.205-220
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• 2004

한국산 광의의 붉나무속(Rhus) 6분류군간의 한계를 검토하기 위하여 28집단에 대한 67개의 외부형태학적 형질을 기초로 주성분분석과 군집분석의 수리분류학적 연구를 실시하였다. 47개의 정량형질을 기초로 한 주성분분석 결과에서는, 주성분 1, 2, 3이 전체분산값의 77.9%(주성분1 35.2%, 주성분2 22.5%, 주성분3 20.2%), 또한 20개의 정성형질을 기초로 한 분석결과에서는, 주성분 1, 2, 3은 전체분산에 대해 90.7%(주성분1 37.7%, 주성분2 33.0%. 주성분3 20.0%)를 설명 할 수 있는 것으로 나타났다. 주성분적재값을 기초로 하여 공간배열을 실시한 결과, 조사된 분류군들은 종집단 간에 뚜렷한 한계를 보이며 유집되었다. 또한, 단순유집계수에 의한 군집분석을 수행하여 UPGMA 표현도를 작성한 결과, 각각의 분류군 사이에는 뚜렷한 한계를 보였다. 군집분석 결과, 한국산 광의의 붉나무속 식물의 분류에는 정성적 형질이 유용한 것으로 나타났으며, 수리분류학적 연구는 한국산 광의의 붉나무속 6분류군의 분류학적 한계설정에 매우 유용한 것으로 나타났다.

• 식물분류학회지
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• 제37권4호
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• pp.477-502
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• 2007

본 연구에서는 Aconitum alboviolaceum Kom. complex에 속하는 6종 중 한국산 4종(A. alboviolaceum, A. longecassidatum, A. pseudolaeve, A. quelpaertense)을 대상으로 형 태 및 주성분분석(Principal Components Analysis)과 엽록체 DNA psbA-trnH IGS, trnL intron 및 tmL-trnF IGS 구간의 분석을 통해 각 분류군의 타당성을 검토하고 그 한계 및 분류학적 위치를 명확히 설정하고자 하였다. 형태분석 결과, 기존에 보고되었던 주요 식별형질에서 형태변이가 종 및 개체군 수준에서 복잡한 형태로 나타났으며 주성분분석 결과 각 분류군들은 종 및 개체군 수준에서 유집 되지 않고 연속적으로 혼합된 양상을 보였다. 염기서열 분석 결과, l0개의 염기치환과 3개의 indel이 관찰되었고, 이를 통해 얻어진 neighbor-joining tree에서 나타난 4개의 그룹은 종 및 개체군을 반영하지 않았다. 따라서, 본 연구에서는 complex 내 분류군들에 관한 기존의 형태형질에 근거한 분류체계를 지지하지 않으며, 이들 분류군 간의 분류체계에 대한 재고가 필요하다고 본다.

• 한국토양비료학회지
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• 제44권5호
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• pp.944-958
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• 2011

Soil hydraulic properties such as hydraulic conductivity or water retention which are costly to measure can be indirectly generated by soil pedotransfer function (PTF) using easily obtainable soil data. The field soil structure description which is routinely recorded could also be used in PTF as an input to reduce the uncertainty. The purposes of this study were to use qualitative morphological soil structure descriptions and soil structural index into PTF and to evaluate their contribution in the prediction of soil hydraulic properties. We transformed categorical morphological descriptions of soil structure into quantitative values using categorical principal component analysis (CATPCA). This approach was tested with a large data set from the US National Pedon Characterization database with the aid of a categorical regression tree analysis. Six different PTFs were used to predict the saturated hydraulic conductivity and those results were averaged to quantify the uncertainty. Quantified morphological description was successively used in multiple linear regression approach to predict the averaged ensemble saturated conductivity. The selected stepwise regression model with only the transformed morphological variables and structural index as predictors predicted the $K_{sat}$ with $r^2$ = 0.48 (p = 0.018), indicating the feasibility of CATPCA approach. In a regression tree analysis, soil structure index and soil texture turned out to be important factors in the prediction of the hydraulic properties. Among structural descriptions size class turned out to be an important grouping parameter in the regression tree. Bulk density, clay content, W33 and structural index explained clusters selected by a two step clustering technique, implying the morphologically described soil structural features are closely related to soil physical as well as hydraulic properties. Although this study provided relatively new method which related soil structure description to soil structure index, the same approach should be tested using a datasets containing the actual measurement of hydraulic properties. More insight on the predictive power of soil structure index to estimate hydraulic properties would be achieved by considering measured the saturated hydraulic conductivity and the soil water retention.

• 한국군사과학기술학회지
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• 제17권6호
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• pp.810-820
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• 2014

Recently many studies of Radar systems mounted on ground vehicles for autonomous driving, SLAM (Simultaneous localization and mapping) and collision avoidance are reported. In near field, several hits per an object are generated after signal processing of Radar data. Hence, clustering is an essential technique to estimate their shapes and positions precisely. This paper proposes a method of grouping hits in range-doppler domains into clusters which represent each object, according to the pre-defined rules. The rules are based on the perceptual cues to separate hits by object. The morphological connectedness between hits and the characteristics of SNR distribution of hits are adopted as the perceptual cues for clustering. In various simulations for the performance assessment, the proposed method yielded more effective performance than other techniques.

• Weed & Turfgrass Science
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• 제6권4호
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• pp.283-291
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• 2017

Zoysiagrass has been used in Korea as a native grass. Informations on distributions, classifications, breeding efforts and outputs, current production and uses of zoysiagrasses in Korea are summarized. During past a few decades, collection of naturally growing zoysiagrass ecotypes has been continued, followed by evaluation, selection and cross-pollination. Classification efforts using important morphological, biochemical traits and DNA polymorphisms resulted in the several suggestions of grouping zoysiagrass species and varieties. Zoysia japonica consistently form separate group, while Zoysia sinica and Zoysia macrostackya have been separately or inter-mixed. Z. matrella with some variations frequently grouped separately but with some similarity with Z. tenuifolia, which was recently renamed as Z. pacifica. Hybrid types of zoysiagrasses were frequently found in Korea. New varieties such as 'Kunhee' and 'Green Ever' etc. were bred by ecotype selections, while 'Senock', 'Semil', 'Millock' were bred through pollination. Molecular approaches were also used to introduce beneficial genes into zoysiagrasses with no practical outputs by legal regulations. Currently popular varieties are inter-specific hybrid types such as 'Anyang' and other local zoysiagrasses as well as common zoysiagrass (Zoysia japonica) Production area in Korea was estimated as 2,947 ha with increasing problems of mixed or contaminated species and varieties.

• Mycobiology
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• 제47권4호
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• pp.408-414
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• 2019

Crinum asiaticum and Hymenocallis littoralis, commonly known as spider lilies are bulbous perennial and herbaceous plants that widely planted in Malaysia as ornamental. During 2015-2016, symptom of leaf blight was noticed on the hosts from several locations in Penang. The symptom appeared as irregular brown to reddish lesions surrounded by yellow halos. As the disease progressed, the infected leaves became blighted, dried, and fell off with the presence of black microsclerotia and pycnidia on the lesions parts. The present study was conducted to investigate the causal pathogen of leaf blight on C. asiaticum and H. littoralis. Based on morphological characteristics and DNA sequences of internal transcribed spacer (ITS) region and translation elongation factor 1-alpha (TEF1-α) gene, the causal pathogen was identified as Macrophomina phaseolina. Phylogenetic analysis of combined dataset of ITS and TEF1-α grouped the isolates studied with other isolates of M. phaseolina from GenBank. The grouping of the isolates was supported by 96% bootstrap value. Pathogenicity test proved the role of the fungus in causing leaf blight on both hosts.

• 식물분류학회지
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• 제49권1호
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• pp.13-27
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• 2019

Numerical taxonomy is employed to determine the phenetic proximity of the Egyptian taxa belonging to the genus Ononis L. A classical clustering analysis and a principal component analysis (PCA) were used to separate 57 macro- and micromorphological characters in order to circumscribe 11 taxa of Ononis. A clustering analysis using the unweighted pair-group method with the arithmetic means (UPGMA) method gives the highest co-phenetic correlation. Results from clustering and PCA revealed the segregation of five groups. Our results are in line, to some certain degree, with the traditional sub-sectional concept, as can be seen in the grouping of the representative members of the subsections Diffusae and Mittisimae together and the representative members of the subsections Viscosae and Natrix. The phenetic uniqueness of Ononis variegata and O. reclinata subsp. mollis was formally established. However, our findings contradict the classic sectional concept; this opinion was suggested earlier in previous phylogenetic circumscriptions of the genus. The most useful characters that provide taxonomic clarity were discussed.

• 한국토양비료학회지
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• 제40권4호
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• pp.262-268
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• 2007

농업 비점오염원으로부터의 수질 보전이나 수자원 관리는 농업적 관리뿐만 아니라 수질관리 및 수자원 관리를 위해서도 유역단위 특히, 소유역의 토양특성을 포괄하는 단위로 체계적으로 분류할 필요성이 있다. 우리나라의 남서쪽에 위치한 영산강유역의 50개 소유역을 대상으로 토양도, 지형도, 하천도 및 유역도를 이용하여 만곡도, 산림의 비율, 평탄지의 비율, 다른 소유역으로부터의 유입 여부 등 토양학적으로 중요한 4개의 특성을 이용하여 군집분석을 수행하였다. 그 결과 5개의 군으로 구분할 수 있었으며, 이 구분의 적합도를 검정하기 위하여 Mantel test를 한 결과 r = 0.83으로 나타나 적합하다는 결론을 얻었다. 이와 같이 토양과 지형특성을 포괄하는 소유역의 분류 및 유사성에 따른 그룹화는 농업에서의 최적영농관리나 오염물질에 따른 수질관리, 수문모형의 적용성 확대 및 수자원 관리에 합리적 유용성을 제공할 것이며 체계적 관리의 밑바탕이 될 것이다.

• Parasites, Hosts and Diseases
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• 제36권2호
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• pp.69-80
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• 1998

가시아메바 (Aconthamoeba) 속의 종 동정과 분류 체계를 확립하기 위해 18종의 대표주를 포함 한23분리주들의 185rRNA 유전자를 PCR로 증폭하고, RFLP를 비교 분석하는 riboprinting을 시행하였다. 이에 근거한 dendrogram은 형태학적 일 grouping과 잘 부합하였다. 형태학적 제1군에 속 한 별가시아메바와 A.tubiahi는 형태학적 제2군 및 제3군의 분리주들로부터 가장 먼저 분지해 나왔으며, 서로간에도 매우 큰 유전적 거리를 나타내었다. 형태학적 제3군의 4분리주 중 A. cuLbertsonj A. hedvi 및 A.pulesti 는neAfsis는 서로간에 큰 유전적 거리를 나타내어 합당한 분류로 인정할 수 있었으나, A. Ppustulosa는 A. pqlestinenis와 유전적으로 매우 가깝게 나타나 A. pdestinensis로 재동정되어야 할 것으로 판단되었다. 형태학적 제2군의 분리주들은 0.2%의 유전 적 거리를 기준으로 하여 6개의 분지군으로 나누어졌다. 그 중 18S rRNA 유전자 내에 intron을 포함하고 있는 A. griffini가 나머지 분리주들과 가장 큰 유전적 거리를 나타내었다. 카스텔라니가시 아메바 Castellani주, Ma주, A. quplnvi13주, 담수가시아메바 L3a주, 대식가시아메바 Jones주 및 A. triangdris SH621주가 하나의 분지군을 형성하였으며, 그 중 A. quina Vil3와 담수가시아메바 L3a주는 카스텔라니가시아메바로 재동정되어야 할 것으로 판단되었다. Chang주는 A. hntchetti로, A. mcuritaniensis, A. divionensis와 A. puraAivionenis는 A. rhysodes로 재동정되어야 할 것으로 판단되었다. Neff주는 카스텔라니가시아메바보다는 대식가시아메바와 유전적으로 훨씬 가까웠다. Riboprinting은 임상 및 환경에서 분리되는 가시아메바 분리주의 빠른 동정에도 유용할 것으로 사료된다.