• 한국초지조사료학회지
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• 제35권4호
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• pp.283-289
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• 2015

수확시 숙기와 조제방법이 호밀 라운드베일 사일리지의 건물 손실률과 형태변화에 대한 영향을 구명하기 위하여 3가지 시험을 수행하였다. 호밀은 3번의 생육단계에 맞춰 수확하였으며 (수잉기, 출수기 및 개화기) i) 예건 (비예건, 단기예건, 장기예건)에 따른 변화, ii) 유산균첨가제 (무처리, 첨가제 A 및 첨가제 B) 처리에 따른 변화, iii) 비닐색 (백색, 흑색, 녹색)에 따른 변화를 관찰하였다. 수잉기 및 비예건 처리구에서 저장 2개월 후의 형태 변화가 심하게 나타났다. 그러나 출수기 이후의 수확 처리구에서는 변화정도가 심하지 않았다. 첨가제 처리는 사일리지의 형태변화와 건물 손실률을 감소시켰다. 건물 손실률은 수확이 지연되거나 첨가제 처리로 유의적으로 감소되었다. 수잉기 처리에서는 형태변화가 심하게 관찰이 되었는데 첨가제 처리구가 비 처리구보다 형태변화가 더 심하게 일어났다. 흑색비닐 처리는 다른 비닐색에 비해 형태변화가 심하게 일어났 으나 건물 손실률은 비닐색에 따른 차이가 없었다. 상관관계에서는 수확시 숙기와 예건기간이 형태변화와 정의 상관이 있는 것으로 나타났다. 이상의 결과를 종합하여 볼 때 건물함량은 사일리지의 형태변화에 영향을 주는 가장 큰 요인으로 나타났다.

• Korea-Australia Rheology Journal
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• 제14권1호
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• pp.11-16
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• 2002

Cephalosporium acremonium is a filamentous microorganism producing cephalosporin C. The morphological differentiation of C. acremonium in submerged culture is closely related with the rheological properties of culture broth and production of cephalosporin C. In this study, the rheological and morphological properties of culture broth of C. acremonium were investigated. In the seed broths of shake-flask and fermenter culture, the Herschel-Berkley equation was in excellent agreement with experimental results in the whole range of shear rate. In the seed broths of shake-flask culture, morphological differentiation into arthrospores affected to changes of apparent viscosity. But results in the fermenter culture, morphological factors such as mean hyphal thickness and the number of tips gave more effect on changes of apparent vitacosity than differentiation into arthrospores. Overall, it suggested that the morphological parameters measured by image analysis can be used as a good parameter to indicate the rheological properties of culture broth of C. acremonium M25.

• Korea-Australia Rheology Journal
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• 제17권1호
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• pp.15-20
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• 2005

In this study, the relationship between morphology and viscosity of the main culture broth of Cephalosporium acremonium M25 was investigated in a 2.5 L bioreactor. The differentiation of C. acremonium M25 showed a complex pattern during the main culture. The morphological changes of C. acremonium M25 were related to the rheological properties of the culture broth and it was well agreed with the power law model. As a result of rheology study, it was found that rheological properties of the main culture broths of C. acremonium M25 in bioreactor were closely related to morphological changes. Also, fractal dimension fairly predicted morphological and rheological changes in the main culture broth.

• International Journal of Oral Biology
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• 제40권1호
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• pp.35-39
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• 2015

Minimal inhibitory concentration (MIC) is the lowest concentration of antibiotics that inhibits the visible growth of bacteria. It has been reported that sub-MIC of antibiotics may result in morphological alterations, along with the biochemical and physiological changes in bacteria. The purpose of this study was to examine morphological changes of Aggregatibacter actinomycetemcomitans, after the treatment with sub-MIC metronidazole and penicillin. The bacterial morphology was observed with scanning electron microscope, after incubating with sub-MIC antibiotics. The length of A. actinomycetemcomitans was increased after the incubation with sub-MIC metronidazole and penicillin. Sub-MIC metronidazole and penicillin inhibited bacterial division and induced long filaments. Our study showed that metronidazole and penicillin can induce the morphological changes in A. actinomycetemcomitans.

• 한국해안해양공학회지
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• 제10권4호
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• pp.187-196
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• 1998

해안 구조물인 돌제(groins)의 축조로 인한 해안 지형 변화를 수치모델을 통하여 살펴보았다. 수치모델은 크게 파랑과 흐름을 계산하는 동수력학적 부분과 이로부터 구해진 외력을 이용하여 지형변화를 계산하는 퇴적물 이동부분으로 구성된다. 본 연구에서 일정한 경사를 갖는 해안에 축조된 한 개의 돌제 및 3개의 돌제 시스템에 수치모델을 적용하여 파랑 및 흐름 양상의 변화로 인한 퇴적물 이동 양상과 150일간에 걸친 해안지형 변화를 계산하였다. 또한 시간에 따른 돌제 통과 퇴적물 이동량 변화도 살펴보았다

• Biomolecules & Therapeutics
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• 제17권4호
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• pp.362-369
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• 2009

We have investigated the effect of glucosamine on the retinal cells after continuous infusion into cerebroventricle by using osmotic minipump to avoid peripheral effect. Continuous intracerebroventricular (i.c.v) infusion of glucosamine with the rate of 0.1 ${\mu}mol$/10 ${\mu}l$/hr for 7 days resulted in morphological changes of the optic nerve in electron microscopic level as well as morphological changes of the retina in light microscopic level. Retinal sections were immunostained for the detection of morphological changes of astrocytes. GFAP immunoreactivity appeared not only in the Muller cells but also many of the radial processes of Muller cells. The optic nerve showed deformed axon and slight lamellar separation of myelin sheath after continuous infusion of glucosamine in observing with electron microscope. Interestingly, vacuoles were observed in deformed axons and retinal layers were folded and detached. These results suggested that glucosamine plays a role in induction of morphological dysfunction in retina and optic nerves.

• The Korean Journal of Physiology and Pharmacology
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• 제5권6호
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• pp.467-477
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• 2001

We examined astrocyte regional heterogeneity in their morphological changes in response to various stimuli. Astrocytes were cultured from six different neonatal rat brain regions including cerebral cortex, hippocampus, cerebellum, mid brain, brain stem and hypothalamus. Astrocyte stellation was induced by serum deprivation and the maximum stellation in different regional astrocytes was achieved after 2 h. After 24 h, in all astrocyte cultures, the level of stellation returned to their original level. Cerebellar or hypothalamic astrocytes were the most or the least sensitive, respectively, to serum deprivation. The order of maximum sensitivity to serum deprivation among different regional astrocytes was: cerebellum＞mid $brain{\ge}hippocampus,\;brain\;stem{\ge}cerebral$ cortex＞hypothalamus. Isoproterenol-induced astrocyte stellation was also examined in different regional astrocytes, and similar order of maximum sensitivity as in serum deprivation was observed. Next a possible developmental effect on astrocyte morphological changes was examined in cerebral cortex and cerebellum astrocytes cultured from postnatal day 1 (P1), P4 and P7 rat brains. A much higher sensitivity of cerebellum astrocytes to serum deprivation as well as isoproterenol treatment was consistently observed in P1, P4 and P7-derived astrocytes compared to cerebral cortex astrocytes. The present study demonstrates different regional astrocytes maintain different levels of morphological plasticity in vitro.

• Biomolecules & Therapeutics
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• 제21권6호
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• pp.447-453
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• 2013

Chondroitin sulfate proteoglycan (CSPG) inhibits neurite outgrowth of various neuronal cell types, and CSPG-associated inhibition of neurite outgrowth is mediated by the Rho/ROCK pathway. Mesenchymal stromal/stem cells (MSCs) have the potential to differentiate into neuron-like cells under specific conditions and have been shown to differentiate into neuron-like cells by co-treatment with the ROCK inhibitor Y27632 and the hypoxia condition mimicking agent $CoCl_2$. In this study, we addressed the hypothesis that a ROCK inhibitor might be beneficial to regenerate neurons during stem cell therapy by preventing transplanted MSCs from inhibition by CSPG in damaged tissues. Indeed, dose-dependent inhibition by CSPG pretreatment was observed during morphological changes of Wharton's jelly-derived MSCs (WJ-MSCs) induced by Y27632 alone. The formation of neurite-like structures was significantly inhibited when WJ-MSCs were pre-treated with CSPG before induction under Y27632 plus $CoCl_2$ conditions, and pretreatment with a protein kinase C inhibitor reversed such inhibition. However, CSPG treatment resulted in no significant inhibition of the WJ-MSC morphological changes into neuron-like cells after initiating induction by Y27632 plus $CoCl_2$. No marked changes were detected in expression levels of neuronal markers induced by Y27632 plus $CoCl_2$ upon CSPG treatment. CSPG also blocked the morphological changes of human bone marrow-derived MSCs into neuron-like cells under other neuronal induction condition without the ROCK inhibitor, and Y27632 pre-treatment blocked the inhibitory effect of CSPG. These results suggest that a ROCK inhibitor can be efficiently used in stem cell therapy for neuronal induction by avoiding hindrance from CSPG.

• Applied Biological Chemistry
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• 제34권4호
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• pp.379-385
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• 1991

12품종의 쌀로 부터 분리 정제한 전분의 2.5% (w/v) 현탁액을 각각 $65^{\circ}C$에서 $95^{\circ}C$까지 $5^{\circ}C$ 간격으로 30분간 가열 조리한 후, 전분 입자들의 형태학적 변화를 주사현미경으로 관찰 하였다. 품종에 관계없이 쌀 전분 입자들은 온도가 증가함에 따라 점진적인 형태학적 변화를 보여주었다. 이들 형태학적 변화는 전분의 팽윤도, 용해도 및 아밀로그라프점성 특성등과 밀접한 연관성을 보였다. 전분 입자로 부터 가용성 물질이 용출되는 것과 팽윤된 전분 입자가 그 자체의 독특한 형태를 잃고 서로 망상구조로 연결되면서 이들 리올로지 특성들의 급격한 증가를 가져왔다. 초기 팽윤 단계에서 전분 입자들의 독특한 구조손실 시기는 각각의 호화온도에 영향을 받았다. 호화 온도 이상의 높은 온도에서 전분 입자들이 삼차원적 망상구조를 형성하는 정도는 아밀로오스 함량이 감소할 수록 망상구조가 더 세밀해지며, 증가하는 경향을 보였다.

• 한국지역지리학회지
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• 제13권2호
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• pp.119-128
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• 2007

입도경향분석은 퇴적물 입도경향으로부터 퇴적물의 순이동방향을 도출하는 방법론이다. 입도경향으로부터 도출된 퇴적물의 순이동방향이 낙동강 하류의 삼각주연안 사주점의 지형변화 패턴과 잘 부합한다면 입도경향분석은 삼각주연안 사주섬의 지형변화를 연구하는 과정에서 효과적인 접근법이 될 수 있을 것이다. 이 연구는 Gao and Collins(1992)의 '퇴적물 이동벡터'법에 따라 도출한 퇴적물 순이동방향이 낙동강의 삼각주연안 사주섬의 지형변화를 이해하는 데 적절히 활용될 수 있는지를 검토한 것이다. 연구결과 퇴적물 이동벡터로부터 얻어진 퇴적물 순이동방향은 대체로 항공사진을 통해 추출한 지형변화 패턴를 반영하고 있음을 확인하였으나 지형변화가 급속히 일어나는 지역에서는 그렇지 않았다. 이는 입도경향분석을 통해 얻은 퇴적물 순이동방향이 대표하는 프로세스의 시간적 규모와 지형변화 분석자료의 시간적 규모, 지형변화율 등의 요소의 부합, 불부합 여부 등이 관련되어 있다고 판단된다. 입도경향분석을 낙동강 삼각주연안 사주섬에 적용하고자 할 경우 설명가능한 시간적 규모를 고려한 신중한 접근과 해석이 필요하다고 사료된다.