• Title/Summary/Keyword: Morphological and molecular analyses

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New Record of a Sea Urchin Echinometra mathaei (Echinoidea: Camarodonta: Echinometridae) from Jeju Island, Korea and Its Molecular Analysis

  • Lee, Taek-Jun;Shin, Sook
    • Animal Systematics, Evolution and Diversity
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    • v.28 no.3
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    • pp.178-184
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    • 2012
  • Echinoids were collected at depths of 5-10 m in Munseom, Jeju Island by SCUBA diving on November 23, 2008 and September 15, 2009. Two specimens were identified as Echinometra mathaei (Blainville, 1825) based on morphological characteristics and molecular analyses of mitochondrial cytochrome c oxidase subunit I partial sequences. Echinometra mathaei collected from Korea was redescribed with photographs and was compared with other species from GenBank based on molecular data. Phylogenetic analyses showed that no significant differences were between base sequences of E. mathaei from Korea and that from GenBank. To date, 13 echinoids including this species have been reported from Jeju Island, and 32 echinoids have been recorded in Korea.

Molecular and Morphological Characterization of Endophytic Heterobasidion araucariae from Roots of Capsicum annuum L. in Korea

  • Paul, Narayan Chandra;Deng, Jian Xin;Shin, Kyu-Seop;Yu, Seung-Hun
    • Mycobiology
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    • v.40 no.2
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    • pp.85-90
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    • 2012
  • A species of Heterobasidion was encountered during a diversity study of endophytic fungi from healthy root tissues of chili pepper (Capsicum annuum L.) in Korea. The fungal species (CNU081069) was identified as Heterobasidion araucariae based on phylogenetic analyses of the internal transcribed spacer and translation elongation factor gene sequences. Morphological descriptions of the endophytic isolate matched well with the previous references and supported the molecular identification. The fungus Heterobasidion araucariae CNU081069 is new to Korea.

Four unrecorded species of macrofungi from South Korea

  • Sung-Eun Cho;Dong-Hyeon Lee;Eui Kyeong Kim;Keum Chul Shin;Nam Kyu Kim
    • The Korean Journal of Mycology
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    • v.51 no.3
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    • pp.167-177
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    • 2023
  • In 2022, our investigation focused on exploring the macrofungal diversity in South Korea. Through the examination of morphological characteristics and ITS sequences of the rDNA, we successfully identified four unrecorded species (Laccaria striatula, Leotia atrovirens, Malvipezia emileia, Xerula strigosa). Detailed morphological descriptions, accompanied by photographs and molecular phylogenetic analyses were performed.

Phylogenetic position of eight Amphora sensu lato (Bacillariophyceae) species and comparative analysis of morphological characteristics

  • Wang, Pengbin;Park, Bum Soo;Kim, Jin Ho;Kim, Joo-Hwan;Lee, Hae-Ok;Han, Myung-Soo
    • ALGAE
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    • v.29 no.2
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    • pp.57-73
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    • 2014
  • Amphora Ehrenberg ex Kutzing sensu lato is a common and widespread benthic diatom genus with a taxonomy that has been under continual revision, particularly based on molecular analyses. Although Amphora species have been studied using modern microscopy in recent years, there has not been much progress on molecular characterization of the species, especially in Asia. In this study of Amphora, sampling was carried out from September 2009 to August 2010 in Korean coastal waters. The morphological and molecular characteristics of eight Amphora sensu lato were examined: Amphora marina, A. proteus, Halamphora costata, H. coffeaeformis, H. eunotia, H. holsatica, H. terroris, and Halamphora sp. Based on previous accounts, morphology suggested that A. marina and A. proteus belong to the subgenus Amphora Cleve, which have smooth girdle bands and rather coarse and very distinct areolae on the valve. The other species, H. coffeaeformis, H. costata, H. eunotia, H. holsatica, H. terroris, and Halamphora sp. belong to the subgenus Halamphora Cleve, which was recently elevated to generic status by Levkov 2009, have plicate girdle bands, puncta which do not form straight longitudinal lines, valves which have a narrow ventral portion and apices that are generally rostrate-capitate and recurved. In agreement with analysis based on morphological characteristics, phylogenetic analysis based on small subunit rDNA suggested that the eight Amphora sensu lato species were not a monophyletic group as the morphological classification. Also, the results of molecular work and statistical analysis on all these Amphora sensu lato combined with phylogenic analysis on our geographically representative samples give strong evidence that Halamphora Levkov is independent of Amphora Cleve. Furthermore, in this study, Amphora terroris was transferred Halamphora as Halamphora terroris (Ehrenberg) Wang comb. nov. and Amphora marina was recorded for the first time in Korea.

Genetic Distances Among Rice Mutant Genotypes Assessed by AFLP and Aluminum Tolerance-Related Traits

  • Malone, Emilia;Kopp, Mauricio Marini;Malone, Gaspar;Branco, Juliana Severo Castelo;Carvalho, Fernando Iraja Felix;Oliveira, Antonio Costa de
    • Journal of Crop Science and Biotechnology
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    • v.10 no.2
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    • pp.106-111
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    • 2007
  • Increasing genetic variability with mutagenic agents has been broadly employed in plant breeding because it has the potential to alter one or more desirable traits. In this study, a molecular analysis assessed by Amplified Fragment Length Polymorphisms(AFLPs) and a morphological analysis based on seedlings subjected to aluminum stress were compared. Also, an analysis of allelic frequencies was performed to observe unique alleles present in the pool. Genetic distances ranging from 0.448 to 0.953 were observed, suggesting that mutation inducing was effective in generating variability. The genetic distances based on morphological data ranged from 0(genotypes 22 and 23) to 30.38(genotypes 15 and 29). In the analysis of allelic frequency, 13 genotypes presented unique alleles, suggesting that mutation inducing was also targeting unique sites. Mutants with good performance under aluminum stress(9, 15, 18 and 27) did not form the same clusters when morphological and molecular analyses were compared, suggesting that different genomic regions may be responsible for their better performance.

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Morphological and Molecular Characterization of the Newly Reported Penicillium pimiteouiense from Field Soil in Korea

  • Mahesh Adhikari;Hyun Seung Kim;Hyun Seung Kim;Ki Young Kim;In Kyu Lee;Eun Jeong Byeon;Ji Min Woo;Hyang Burm Lee;Youn Su Lee
    • The Korean Journal of Mycology
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    • v.50 no.3
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    • pp.205-215
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    • 2022
  • Penicillium pimiteouiense was discovered in South Korea during an investigation of fungal communities in soil collected from the Gyeongsangbuk-do province. In this study, we performed molecular analysis of this fungal isolate using internal transcribed spacer rDNA, β-tubulin, and Calmodulin gene sequences. We also performed morphological analysis using five agar media, potato dextrose, oatmeal, malt extract, czapek yeast extract, and yeast extract sucrose. In this study, the molecular and morphological analyses of P. pimiteouiense with detailed descriptions and figures has been carried out.

New record of two marine ulvalean species (Chlorophyta) in Korea

  • Lee, Seung Hee;Kang, Pil Joon;Nam, Ki Wan
    • Journal of Ecology and Environment
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    • v.37 no.4
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    • pp.379-385
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    • 2014
  • Two marine ulvalean species (Chlorophyta) were collected from the southern and eastern coasts of Korea. One is morphologically characterized by usually unbranched or little branched ribbon-shaped thalli, fronds with spirally twisted basal portions and usually undulate margins and 1 (-2) pyrenoids per cell. The other has irregularly shaped thalli, undulate and dentate margins in the fronds, small macroscopic denticulations along the margin and (1-) 2-3 pyrenoids per cell. In phylogenetic tree based on molecular data, the two species nest in the same clade with Ulva flexuosa and U. rigida, respectively. These two Korean entities are identified as U. flexuosa and U. rigida, respectively, based on morphological and molecular analyses. This is the first record of Ulva flexuosa and U. rigida in Korea.

A new record of Feldmannia chitonicola from Korea based on laboratory culture and molecular data

  • Avila-Peltroche, Jose;Oteng'o, Antony Otinga;Jeong, So Young;Won, Boo Yeon;Cho, Tae Oh
    • Korean Journal of Environmental Biology
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    • v.37 no.3
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    • pp.278-284
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    • 2019
  • Feldmannia chitonicola is reported as a new record from Korea based on morphological studies in laboratory-cultured materials and molecular analyses. F. chitonicola is mainly characterized by a small size (1-2 mm), erect filaments mostly unbranched, plurilocular sporangia produced on both prostrate and laterally on the basal part of erect filaments, and 2 types (lanceolate and ovate) of sporangia. In our cultures, sporangia production was slower at 10℃ than in 16℃ and 20℃. Our molecular analyses of rbcL and cox1 genes supported its independence from other congeners reported for Korea. This is the first report of F. chitonicola for western Pacific.

Ralfsia longicellularis (Ralfsiales, Phaeophyceae): a Far East Asian endemic brown alga from Korea

  • Oteng'o, Antony Otinga;Won, Boo Yeon
    • Korean Journal of Environmental Biology
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    • v.38 no.1
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    • pp.101-105
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    • 2020
  • Ralfsia longicellularis is known as an endemic species in Far East Asia. In this study, we report R. longicellularis as a new record from Korea based on morphological and molecular analyses. Molecular analyses based on plastid-encoded rbcL gene sequences and morpho-anatomical studies were undertaken on Ralfsia species, a poorly studied genus from Korea. Ralfsia longicellularis is mainly characterized by a dark brown thallus; 770-1200 ㎛ thick, curved cells in the creeping and ascending parts of the filaments; basal layer and erect filament cells with a width-to-length ratio of 1 : 1.5 to 10; narrowshaped sporangia on-stalk cells at the base of the paraphyses; and mostly uniseriate plurilocular reproductive organs capped with 1-2 sterile cells. The R. longicellularis samples from Korea in this study were similar to ones collected from the type locality (Peter the Great Bay, Russia) in morphology. The rbcL analyses also revealed that our Korean R. longicellularis samples were placed in the same clade with Russian materials, within a Ralfsia clade but distinct from the congeners.

Monostroma alittorale, a marine green algal species newly recorded in Korea

  • An, Jae Woo;Kang, Pil Joon;Nam, Ki Wan
    • Korean Journal of Environmental Biology
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    • v.37 no.3
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    • pp.362-366
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    • 2019
  • A marine green algal species (Chlorophyta) was collected from the eastern coast of Korea. It is morphologically characterized by monostromatic thallus, usually undulate and entire margins, cap-like chloroplast and several pyrenoids per cell. In a phylogenetic tree based on molecular data, the Korean alga nests in the same clade as Monostroma alittorale originally described from Japan, as a sister clade of M. grevillei from France. The genetic distance for ITS(Internal Transcribed Spacer) sequences among Monostroma species ranges from 2.3% to 38.2%. The value between the Korean entity and M. alittorale was calculated as 0.01%, considered to be intraspecific divergence. This Korean entity is identified as Monostroma alittorale based on morphological and molecular analyses. This is the first record of M. alittorale in Korea.