• Proceedings of the Korean Society of Crop Science Conference
• /
• 2022.10a
• /
• pp.79-79
• /
• 2022

Common Buckwheat is a crop with high nutritional value due to its high protein, magnesium, iron, and vitamin content, as well as excellent rutin and quercetin content. This study was conducted to investigate the main agricultural characteristics of common buckwheat genetic sources and use them as basic data for establishing cultivation technology and fostering new varieties. The seeds were planted in the research field of Chungbuk National University on 24th April, 2022 maintained at 15 × 20 cm planting spaces. To evaluate the genetic diversity and morphological traits of buckwheat, different kinds of qualitative and quantitative traits were investigated of the 50 varieties. The germination percentage of common buckwheat showed more than 85% that also showed the uniform germination. During the flowering period, all varieties bloomed within six days from June 18 to 23. The leaves color of common buckwheat were all dark green, and most of the stem colors were mixed with light green, light red, red, and crimson. The leaves of common buckwheat were mainly heart-shaped, and most of the flowers were white. The leaf length was 65.78 to 40.53 mm, and the width was 74.42 mm to 39.74 mm. The stem thickness was between 3.5 and 3.76 mm and the length was between 97.3 cm and 40.24 cm.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2021.04a
• /
• pp.9-9
• /
• 2021

Mutation breeding is useful for improving agronomic characteristics of various crops. In this study, we constructed soybean Mutant Diversity Pool (MDP) from 1,695 gamma-irradiated mutants through two selection phases over M1 to M12 generations; we selected 523 mutant lines exhibiting at least 30% superior agricultural characteristics, and, second, we eliminated redundant morphological phenotypes in the M12 generation. Finally, we constructed 208 MDP lines and investigated 11 agronomic traits. We then assessed the genetic diversity and inter-relationships of these MDP lines using target region amplification polymorphism (TRAP) markers. Among the different TRAP primer combinations, polymorphism levels and PIC values averaged 59.71% and 0.15, respectively. Dendrogram and population structure analyses divided the MDP lines into four major groups. According to an analysis of AMOVA, the percentage of inter-population variation among mutants was 11.320 (20.6%), whereas mutant inter-population variation ranged from 0.231 (0.4%) to 14.324 (26.1%). Overall, the genetic similarity of each cultivar and its mutants were higher than within other mutant populations. In an analysis of the genome-wide association study (GWAS) using based on the genotyping-by-sequencing (GBS), we detected 66 SNPs located on 13 different chromosomes were found to be highly associated with four agronomic traits: days of flowering (33 SNPs), flower color (16 SNPs), node number (6 SNPs), and seed coat color (11 SNPs). These results are consistent with those previously reported for other genetic resource populations, including natural accessions and recombinant inbred line. Our observations suggest that genomic changes in mutant individuals induced by gamma rays occurred at the same loci as those of natural soybean population. This study has demonstrated that the integration of GBS and GWAS can serve as a powerful complementary approach to gamma-ray mutation for the dissection of complex traits in soybean.

• Asian Journal of Turfgrass Science
• /
• v.22 no.1
• /
• pp.13-24
• /
• 2008

This study was carried out to provide basic information for the breeding of zoysiagrass synthetic varieties. For estimation of flowering periods, genetic characteristics, and cross-pollination rate, 5 combinations of zoysiagrass breeding lines were compared. Days to stigma emergence and anther exertion were observed in the field to calculate overlapping dates for cross-fertilization. Harvested seed from cross breeding combinations were planted to compare genetic characteristics using morphological traits of progenies. These data were used for determination of 7 phenotypic inheritance types with 8 morphological traits. Cross-pollination rates in 3 combinations of zoysiagrasses were estimated by using lower part color of grass shoots. Cross-pollination rates of zoysiagrass ranged from 11.3$\sim$48.9%, which indicated that zoysiagrass is an allogamous plant. When zoysiagrass breeding lines are properly combined, they may result in valuable synthetic cultivars.

• Journal of Korean Society of Forest Science
• /
• v.99 no.4
• /
• pp.638-644
• /
• 2010

This study was carried out to establish the informations on the optimum container for production of Larix leptolepsis container seedlings. Height growth of 1-year-old container seedling was highest in 250 mL of container (19.3 cm), and of 2-year-old seedling was in 500 mL (56.9 cm). On the other hand, the highest growth of root-collar diameter was observed in the both 1-year-old and 2-year-old seedlings grown in 500 mL. Dry mass production in both a whole seedling and each organs of 2-year-old seedling was significantly high in the container with larger volume and lower seedling density. According to the analysis using by WinRhizo program, the larger container in volume showed better root morphological traits such as total root length, root projected area, root surface area, root volume and average root diameter. Among the elements for analysis of root morphological traits, the root volume was the highest one affected by container volume. And it was observed that the root volume was 79.5% and 46.8% in 320 mL and 250 mL, respectively, compared to 500 mL.

• Korean Journal of Breeding Science
• /
• v.42 no.4
• /
• pp.413-418
• /
• 2010

A total of 424 plants was regenerated from the seed-culture of a rice cultivar, 'Ilpum'. The regenerated plants were grown in a greenhouse. The 297 plants with high fertility were selected among 424 plants. The harvested seeds from each plant were planted to each line at experiment field in 2008 and 2009. The each line was evaluated for the agronomic and morphological traits, also. The 64 lines (21.5%) showed significant differences in agronomic and morphological traits from donor cultivar 'Ilpum' among 297 lines. The heading date different from donor cultivar 'Ilpum' showed highest frequency in 297 lines, and accounts for 9.1% (29 lines). The phenotype of opaque endosperm and rolling leaf account for 1.7% and 1.3% in 297 lines, respectively. The genetic segregation was observed in dwarf/semi-dwarf, rolling leaf and opaque endosperm at $S_1$ generation, but not in $S_2$ generation. These results suggest that the mutant derived from a tissue-culture will be one of the promising genetic resources, due to its wide variation and high frequency of mutation, comparatively.

• ALGAE
• /
• v.29 no.4
• /
• pp.267-277
• /
• 2014

Green tides occur every year in the Yellow Sea (YS), and numerous investigations are proceeding on various aspects of the phenomenon. We have identified bloom-forming species collected from diverse locations in the YS using morphological traits and the chloroplast gene for the large subunit of ribulose-1,5-bisphosphate carboxylase (rbcL). Morphological and rbcL sequence data analyses characterized the blooming species on both sides of the YS as belonging to the Ulva linza-procera-prolifera (LPP) complex clade or U. prolifera of earlier reports. However, U. procera within the LPP complex must be regarded as synonym of U. linza. Moreover, U. prolifera in free-floating samples collected from the Qingdao coast in 2009 was clearly in a distinct clade from that of the blooming species. Therefore, U. linza is the main green tide alga in the YS and has the procera-morphology. The green drift mats in the southeastern part of the YS (southwest sea of Korea) consisted predominantly of U. linza and rarely of U. compressa or U. prolifera.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.54 no.4
• /
• pp.422-426
• /
• 2009

Sweetpotato is one of the important starch crops, current more considered as an industrial crop rather than food because it has higher starch content (over 80% of biomass), it is used for bio resources for industrial area. In this study, we generated S598A (a mutant gene of oat phytochrome A) sweetpotato plant using Agrobacterium-transformation method. Morphological characteristics of S598A plant were compared with the wild type sweetpotato, S598A had darker green leaves, increased chlorophyll content higher than to two-fold, delayed leaf senescence, shorter plant height (60% shorter than that of the wild type), more number of leaves and petioles about 1.8-fold, shorter petiole length (30% shorter), 1.2-fold more branches and 1.6-fold thicker stem diameters. From this study, S598A plants with such phenotypic characteristics might be able to use the solar energy efficiently, to have increased tolerance to biotic and abiotic stresses and finally to increase productivity (not only starch yield but also root biomass yield). S598A sweetpotato lines are under field trials.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.9 no.1
• /
• pp.79-87
• /
• 2004

The silkworm rearing and growth parameters of 63 multivoltine silkworm accessions under extended period of egg preservation at 5$^{\circ}C$ from 30 days to 45 days were studied. The results indicate that, nine accessions did not respond to extended period of egg preservation at low temperature and the remaining 54 accessions responded to the treatment and three rearings were conducted for comparision with the control; to estimate the effect of prolonged egg preservation at low temperature. The non-parametric tests statistics (Wilcoxon tests) was adopted for comparing the mean performance of treated batches (45 days) over the control (30 days). Highly significant variability was found among the accessions for all the parameters under study. The genetically controlled morphological characters were not altered in the treated batches, which were found to be on par with that of control. However, the total larval duration varied significantly over the control in 51 accessions. Similarly, the fifth age larval duration of 27 accessions showed decreasing trend compared to control. Altogether 41 accessions were found to be tolerant to long-term cold preservation upto 45 days, without showing any significant variation for morphological as well as essential quantitative traits. These accessions may be recommended for long-term egg preservation schedule up to 45 days, which will reduce the cost of conservation of these silkworm germplasm.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.49 no.2
• /
• pp.198-207
• /
• 2016

Tanaka’s snailfish, Liparis tanakae (Gilbert and Burke, 1912), is distributed throughout the coasts of Korea. To clarify the population structure of L. tanakae, we analyzed the morphological and genetic variation among individuals sampled from three localities surrounding the Korean peninsula: Boryeong in the Yellow Sea, Jinhae in the Korea Strait and Pohang in the East Sea. Principal component analysis based on 20 morphometric characteristics revealed two slightly distinct groups (Boryeong vs. Jinhae and Pohang). However, canonical discriminant analysis clearly revealed three groups, separated according to locality. Pairwise differentiation index (F_ST) comparisons based on 762-base pairs mitochondrial cytochrome b gene sequences showed that Boryeong significantly differed from Jinhae and Pohang, but Jinhae and Pohang did not significantly differ from each other. Our findings suggest that Korean L. tanakae comprise at least two groups. Further studies using more sensitive DNA markers, such as microsatellite DNA, are required.

• Fisheries and Aquatic Sciences
• /
• v.17 no.4
• /
• pp.491-496
• /
• 2014

A total of six specimens (57.03-100.72 mm in standard length) belonging to the family Diodontidae were collected around Jeju Island, Korea between July and August 2013. On the Basis of morphological and molecular analyses, we identified the specimens as Cyclichthys orbicularis (Bloch, 1785), a new record to the Korean fish fauna. Morphologically, the species is characterized by nine caudal fin rays, immovable spines on the head and body except the caudal peduncle, and small black spots dorsally. Some morphometric characters changed disproportionately with growth, which was a new finding. Caudal peduncle length, body width and preanus length to standard length tend to grow at a rate different from that of overall growth. When the 601 base pairs of mitochondrial DNA cytochrome c oxidase subunit I sequences of our specimens were compared with those of other diodontid fishes, our specimens clustered tightly around C. orbicularis (d = 0.000-0.002) in an NJ tree, with a high bootstrap value (99%). We herein describe the morphological and molecular traits of the specimens, and propose a new Korean name, "gud-ga-si-bok-sok" for the genus Cyclichthys, and "dung-geun-gud-ga-si-bok" for C. orbicularis.