• Proceedings of the Korean Society of Food Hygiene and Safety Conference
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• 2001.10a
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• pp.54-60
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• 2001

• Molecules and Cells
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• v.26 no.3
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• pp.319-322
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• 2008

Little is known about the chromosomal variability and polymorphism existing in mitotic chromosomes of Citrus, mainly due to lack of reliable chromosomal markers and small chromosome size. To test the hypothesis of chromosomal polymorphism and provide the foundation of the genome organization in the Citrus cultivars, we have developed molecular cytogenetic markers for 13 Citrus species collected from Jeju island, Korea. In this study, we demonstrated that the chromosomal locations of cytogenetic markers are quite variable and extremely polymorphic, in contrast to the previous studies. The data obtained in this study will be of utmost importance in cytological systematics and karyotyping of the Citrus species.

• Proceedings of the Korea Environmental Mutagen Society Conference
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• 2001.10b
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• pp.175-175
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• 2001

• The Plant Pathology Journal
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• v.32 no.2
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• pp.112-122
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• 2016

Virus-induced gene silencing (VIGS) is an effective tool for the study of soybean gene function. Successful VIGS depends on the interaction between virus spread and plant growth, which can be influenced by environmental conditions. Recently, we developed a new VIGS system derived from the Soybean yellow common mosaic virus (SYCMV). Here, we investigated several environmental and developmental factors to improve the efficiency of a SYCMV-based VIGS system to optimize the functional analysis of the soybean. Following SYCMV: Glycine max-phytoene desaturase (GmPDS) infiltration, we investigated the effect of photoperiod, inoculation time, concentration of Agrobacterium inoculm, and growth temperature on VIGS efficiency. In addition, the relative expression of GmPDS between non-silenced and silenced plants was measured by qRT-PCR. We found that gene silencing efficiency was highest at a photoperiod of 16/8 h (light/dark) at a growth temperature of approximately $27^{\circ}C$ following syringe infiltration to unrolled unifoliolate leaves in cotyledon stage with a final SYCMV:GmPDS optimal density $(OD)_{600}$ of 2.0. Using this optimized protocol, we achieved high efficiency of GmPDS-silencing in various soybean germplasms including cultivated and wild soybeans. We also confirmed that VIGS occurred in the entire plant, including the root, stem, leaves, and flowers, and could transmit GmPDS to other soybean germplasms via mechanical inoculation. This optimized protocol using a SYCMV-based VIGS system in the soybean should provide a fast and effective method to elucidate gene functions and for use in large-scale screening experiments.

• Journal of East-West Nursing Research
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• v.29 no.2
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• pp.185-195
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• 2023

Purpose: The purpose of this study is to analyze changes of child welfare policies and provide insights based on the collection and classification of newspaper articles. Methods: Articles related to child welfare policies were collected from 1990, during the Kim, Young-sam administration, to May 9, 2022, under the Moon, Jae-in administration. K-Means clustering and keyword Term Frequency-Inverse Document Frequency analysis were utilized to cluster and analyze newspaper articles with similar themes. Results: The administrations of Kim, Young-sam, Kim, Dae-jung, Roh, Moo-hyun, and Park, Geun-hye were classified into two clusters, and the Lee, Myung-bak and Moon, Jae-in administrations were classified into three clusters. Conclusion: South Korea's child welfare policies have focused on ensuring the safety and healthy development of children through diverse policies initiatives over the years. However, challenges related to child protection and child abuse persist. This requires additional resources and budget allocation. It is important to establish a comprehensive support system for children and families, including comprehensive nursing support.

• The Journal of the Korea Contents Association
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• v.22 no.5
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• pp.585-599
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• 2022

This study examines the types and characteristics of the policy instruments of Korea's higher education policies since the Lee Myung-bak administration. To this end, McDonnell and Elmore (1987)'s types of policy instruments were adopted and the higher education policy tasks announced by the Ministry of Education were classified by sector and type. As a result of the analysis, Korea's higher education policy showed a tendency to gradually shift from 'mandates' to 'inducements' and 'capacity building'. Despite this trend, however, in policy tasks such as university evaluation, the Lee Myung-bak, Park Geun-hye, and Moon Jae-in administrations all maintained policy instruments that combine 'mandates' and 'inducements'. Third, the 'system change' based on a market-oriented mechanism was used only in the Lee Myung-bak administration, and was not adopted in the higher education policies of the Park Geun-hye and Moon Jae-in administrations. In the field of global and industry-university cooperation at universities, it is encouraging that the Lee Myung-bak administration to the Moon Jae-in administration have steadily introduced policy instrument of 'capacity building', but in the fields of specialization and competitiveness enhancement, policies centered on 'mandates' and 'inducements' was using the policy instrument.

• The Korean Journal of Emergency Medical Services
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• v.7 no.1
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• pp.179-198
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• 2003

If see disaster administration system of our country, start in terms of is coping by countermeasure after four immediately after disaster occurrence many problems blessing with a sons by tribe and so on of link nature between formation, disaster administration complete charge utensil's absence, disaster charge manpower and budget be indicated and join. If examine improvement way accordingly, is as following ; Necessity of synthetic disaster administration system, Fire fighting formation's independence guarantee, Integration of fire fighting connection similarity business, Disaster administration's permanent establishment complete charge utensil's necessity, Disaster administration midautumn complete charge utensil at a metropolitan autonomous fire fighting system reorganization, Role division of labor between center and local government, Disaster administration professional human strength positivity, Disaster administration information system construction practical use, Equipment and improvement of budget state, Education public information for safety culture consciousness fixing, Internationalization of fire fighting business, globalization propulsion, Structure, member of rescue confrontation system and efficiency. Fire fighting environment is changing greatly, and fire fighting must become center to correspond to do confrontation that do one thing troble when produce disaster.

• THE JOURNAL OF KOREAN ORIENTAL ONCOLOGY
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• v.1 no.1
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• pp.167-190
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• 1995

Bikiwhan is one of the oriental medicines that have been used for the treatment of tumors since ancient times. However, the mechanism of the drug action is not closely surved. This study was made to investigate the effects of Bikiwhan on the innate immunity were analysed by measuring the functions of phagocytes, and those of specific immunity were analysed by measuring T and B cells activities. The followings are the results obtained from this study : 1. Bikiwhan has direct cytotoxic effects against human lymphoma cell lines (K562) in a dose dependent manner. 2. An administration of Bikiwhan increased allogenic immune response in the mouse. 3. An administration of Bikiwhan increased the antibodies formation against SRBC. 4. An administration of Bikiwhan enhanced the apperance of rosette forming cells in the spleen. 5. An administration of Bikiwhan decreased the delayed-type hypersensitivity against dinitrofluorobenzene. 6. An administration of Bikiwhan has no effect on natural killer cells. 7. Bikiwhan increased the phagocyte activity of peritoneal macrophages in vitro and in in vivo as well. 8. Bikiwhan depressed the formation of reactive oxygen intermediated in vitro and in vivo as well. 9. Bikiwhan has the capacity to make peritoneal macrophages secrete nitric oxide. The above results demonstrate that Bikiwhan has enhancing effects of immune responses against tumors by decreasing tissue demages caused by immune responses.

• Horticultural Science & Technology
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• v.31 no.3
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• pp.269-274
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• 2013

To further our understanding of sugar accumulation in 'Shiranuhi' mandarin [(C. unshiu ${\times}$ C. sinensis) ${\times}$ C. reticulate], we investigated the patterns of sugar uptake in juice sacs exposed to different concentrations of sucrose, fructose and glucose in vitro. Data was also collected on the change in weight and shape of the in vitro juice sacs over time. Soluble solids content, sugar content and acidity content were highest at 20% sucrose, fructose and glucose solution content; while fructose content was highest at 5% sucrose concentration. Furthermore, the juice sac's fresh weight was highest at 5% sucrose and lowest at 20% fructose content. The shape of the juice sacs also differed in different sugar concentration and type. Overall, sucrose, fructose and glucose content in juice sacs increased with the sugar concentration. These results suggest that sugar translocation into juice sacs is actively induced by high sugar concentration in the medium. Thus, it can be concluded that sugar and acid accumulation in juice sacs increased with sugar concentration in vitro culture.

• Korean Journal of Agricultural and Forest Meteorology
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• v.20 no.2
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• pp.145-148
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• 2018