• Title/Summary/Keyword: MonA

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Multiplex PCR Detection of the MON1445, MON15985, MON88913, and LLcotton25 Varieties of GM Cotton

  • Kim, Jae-Hwan;Kim, Sun-A;Seo, Young-Ju;Lee, Woo-Young;Park, Sun-Hee;Kim, Hae-Yeong
    • Food Science and Biotechnology
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    • v.17 no.4
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    • pp.829-832
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    • 2008
  • A multiplex polymerase chain reaction (PCR) method was developed to simultaneously detect 4 varieties of genetically modified (GM) cotton. The event-specific primers were used to distinguish the 4 varieties of GM cotton (MON1445, MON15985, MON88913, and LLcotton25) using multiplex PCR. The acyl carrier protein 1 (Acp1) gene was used as an endogenous reference gene of cotton in the PCR detection. The primer pair Acp1-AF/AR containing a 99 bp amplicon was used to amplify the Acp1 gene and no amplified product was observed in any of the 13 different plants used as templates. This multiplex PCR method allowed for the detection of event-specific targets in a genomic DNA mixture of up to 1% GM cotton containing MON1445, MON15985, MON88913, and LLcotton25.

An Edutainment Mon-E Robot for Young Children (유아용 에듀테인먼트 Mon-E로봇)

  • Kim, Jong-Cheol;Kim, Hyun-Ho
    • The Journal of Korea Robotics Society
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    • v.6 no.2
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    • pp.147-155
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    • 2011
  • This paper presents an edutainment robot for young children. The edutainment robot called 'Mon-e' has developed by the Central R&D Laboratory at KT. The main services of the Mon-E robot are autonomous moving service, object card and story book telling service and videophone service. The RFID technology was introduced for easy interface to young children. The face of Mon-E robot is mounted with an RFID reader. The RFID tag is pasted on story book and object card. If you approach a book or an object card to the face of Mon-E, the Mon-E robot recognizes the identified code and plays its service. In autonomous moving, if the Mon-E robot meets obstacles, it moves back and turns left or right or half rotation. In videophone service, if young children approach an RFID card to the Mon-E, the Mon-E can make a call to the specific number, which is contained in the RFID card. The developed Mon-E robot has tested in real world environment and is evaluated young children and their parents. In the result of evaluation, the feeling of satisfaction was high to main services of Mon-E robot.

Mon1 Is Essential for Fungal Virulence and Stress Survival in Cryptococcus neoformans

  • Son, Ye-Eun;Jung, Won-Hee;Oh, Sang-Hun;Kwak, Jin-Hwan;Cardenas, Maria E.;Park, Hee-Soo
    • Mycobiology
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    • v.46 no.2
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    • pp.114-121
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    • 2018
  • Mon1 is a guanine nucleotide exchange factor subunit that activates the Ypt7 Rab GTPase and is essential for vacuole trafficking and autophagy in eukaryotic organisms. Here, we identified and characterized the function of Mon1, an ortholog of Saccharomyces cerevisiae Mon1, in a human fungal pathogen, Cryptococcus neoformans. Mutation in mon1 resulted in hypersensitivity to thermal stress. The mon1 deletion mutant exhibited increased sensitivity to cell wall and endoplasmic reticulum stress. However, the mon1 deletion mutant showed more resistance to the antifungal agent fluconazole. In vivo studies demonstrated that compared to the wild-type strain, the mon1 deletion mutant attenuated virulence in the Galleria mellonella insect model. Moreover, the mon1 deletion mutant was avirulent in the murine inhalation model. These results demonstrate that Mon1 plays a crucial role in stress survival and pathogenicity in C. neoformans.

Conserved Roles of MonA in Fungal Growth and Development in Aspergillus Species

  • Son, Ye-Eun;Park, Hee-Soo
    • Mycobiology
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    • v.47 no.4
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    • pp.457-465
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    • 2019
  • MonA is a subunit of a guanine nucleotide exchange factor that is important for vacuole passing and autophagy processes in eukaryotes. In this study, we characterized the function of MonA, an orthologue of Saccharomyces cerevisiae Mon1, in the model fungus Aspergillus nidulans and a toxigenic fungus A. flavus. In A. nidulans, the absence of AnimonA led to decreased fungal growth, reduced asexual reproduction, and defective cleistothecia production. In addition, AnimonA deletion mutants exhibited decreased spore viability, had reduced trehalose contents in conidia, and were sensitive to thermal stress. In A. flavus, deletion of AflmonA caused decreased fungal growth and defective production of asexual spores and sclerotia structures. Moreover, the absence of monA affected vacuole morphology in both species. Taken together, these results indicate that MonA plays conserved roles in controlling fungal growth, development and vacuole morphology in A. nidulans and A. flavus.

A kinematic study of young stars in Monoceros OB1 and R1 associations

  • Lim, Beomdu;Naze, Yael;Hong, Jongsuk;Yoon, Sungyong;Lee, Jinhee;Hwang, Narae;Park, Byeong-Gon;Lee, Jeong-Eun
    • The Bulletin of The Korean Astronomical Society
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    • v.46 no.2
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    • pp.50.1-50.1
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    • 2021
  • The Gaia mission opens a new window to study the kinematics and dynamics of young stellar systems in detail. The kinematic properties of young stars provide vital constraints on the formation process of their host systems. Here, we present a kinematic study of the two associations Monoceros OB1 (Mon OB1) and R1 (Mon R1). Member candidates are first selected from the published list of member candidates, a compilation of OB star catalogues, and the classification of young stellar objects with the AllWISE data. According to the conventional wisdom, we selected a total of 728 members with similar proper motions at almost the same distance. Mon OB1 and Mon R1 have high levels of substructures that are also kinematically distinct. We identify six stellar groups in these associations, of which five show a pattern of expansion. In addition, the signature of rotation is found in two stellar groups of Mon OB1. Star formation history is inferred from a color-magnitude diagram. As a result, star formation in Mon OB1 has been sustained for several million years, while Mon R1 formed at almost the same epoch as the recent star formation in Mon OB1. Some old members in the outskirt of Mon OB1 have outward motions, which rules out the previously proposed outside-in star formation scenario. Star-forming regions including Mon OB1 and Mon R1 are found along a large arc-like gas structure. Hence, the formation of these two associations may originate from the hierarchical star formation along filaments in a turbulent molecular cloud.

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An Analysis on Water Supply Amount for Economic Plan in School Buildings (학교건축의 적정 급수량 산정을 위한 사용수량 분석)

  • Lee, Jae-Yoon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.4
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    • pp.2924-2932
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    • 2015
  • This study is for estimate the amount of water supplied data to get a database for economic plan of water supply system by survey for the amount of water supplied of primary, middle and highschool. For this, 54 of primary school, middle school, high school in 6 cities of Kangwon for 5 years. As a result, average of the amount of water supplied per month is $600.7m^3/mon$ and primary, middle schools are $484.2m^3/mon$ and high schools are $833.8m^3/mon$. Average of the amount of water supplied per class is $27.0m^3/mon/class$ and primary, middle schools are $24.4m^3/mon/class$ and high schools are $32m^3/mon/class$. Average of the amount of water supplied per person per day is 55.8l/c/d and primary school, middle schools are 54.4l/c/d and high schools are 58.6l/c/d.

A Study on the Prediction of the Octane Number of Gasolines from the Carbon Type Structural Compositions by 13C-Nuclear Magnetic Resonance Spectroscopy (13C-NMR에 의해 결정된 탄소 유형별 구조적 조성으로부터 가솔린 옥탄가의 예측에 관한 연구)

  • Choi, Ju-Hwan;Chun, Yong-Jin;Choi, Ung-Su;Choi, Young-Sang;Kwon, Oh-Kwan
    • Applied Chemistry for Engineering
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    • v.4 no.4
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    • pp.753-759
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    • 1993
  • The research and motor octane numbers (RON & MON, respectively) of a gasoline are dynamic measures of its quality of performance as a fuel. ASTM standard engine test methods (RON:ASTM D-2699, MON:ASTM D-2700) have been used for determining the octane numbers (RON,MON)of gasolines. But these methods have been widely criticized because their repeatability and reproducibility of the test method are very poor. In addition to these objections, the cost and operation time involved in measuring by the standard method led to searches for "non -engine" methods (Gas Chromatographic method, Nuclear Magnetic Resonance Spectroscopic method). In this study, we determined the carbon type structural compositions of the gasolines by $^{13}C-NMR$ spectroscopy and predicted the octane number (RON & MON) with good accuracy. we presented an assessment of the effects of molecular structural composition on octane numbers.

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Meat Quality Traits of Longissimus Muscle of Hanwoo Steers as a Function of Interaction between Slaughter Endpoint and Chiller Ageing

  • Dashdorj, Dashmaa;Oliveros, Maria Cynthia R.;Hwang, In-Ho
    • Food Science of Animal Resources
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    • v.32 no.4
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    • pp.414-427
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    • 2012
  • Carcass characteristics and meat quality traits as a function of endpoint months of slaughter age (26 vs 32 mon) and chiller ageing (1 vs 10 d) were evaluated for m. longissmus of 26 Hanwoo steers fed with commercial diets including whole crop barley silage. Totally twenty six Hanwoo steers for 6 mon of age that were fed until 26 mon of age constituted the short term-fed group and fed until 32 mon of age constituted long-term fed group. Carcasses were chilled for 24 h and were graded. Strip loin samples were divided into two age groups (1 d and 10 d). Long-term feeding increased carcass weight, rib-eye area, yield grade, marbling score, firmness and quality grade of the meat. The feeding for 32 mon produced tender, juicy meat (p<0.01) with lower cooking loss and higher rating score (p<0.05) than short term feeding, while other quality traits were not influenced by the length of feeding. Intramuscular fat content and oxidative stability (TBARS value) were significantly (p<0.05) higher in beef from long-term feeding however the length of feeding did not alter the fatty acid composition. Chiller aging reduced instrumental tenderness (WBSF value), improved color, sensory tenderness, acceptability and rating of beef. The results of the present study mirrors that Hanwoo steers until 32 mon of age overall improved carcass traits and palatability compared to that for 26 mon. However, from the viewpoints of economical and environmental aspects, cost of the additional feeding for 6 mon for value-adding of eating quality was relatively high and the effects in turn were limited.

Effect of Feed Additives in Growing Lambs Fed Diets Containing Wet Brewers Grains

  • Aguilera-Soto, J.I.;Ramirez, R.G.;Arechiga, C.F.;Mendez-Llorente, F.;Lopez-Carlos, M.A.;Silva-Ramos, J.M.;Rincon-Delgado, R.M.;Duran-Roldan, F.M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.10
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    • pp.1425-1434
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    • 2008
  • The study was conducted to evaluate and compare the effects of feed additives on ruminal fermentation, nutrient digestibility and performance of lambs fed diets containing 60% wet brewers grains (WBG). In Experiment 1, two simultaneous trials were conducted. Fifty intact ($20.2{\pm}0.8kg\;BW$) lambs were used in a feedlot trial and 10 (rumen cannulated; $32{\pm}1kg\;BW$) in a digestion trial. The pH, volatile fatty acids (VFA) and ammonia-N in lambs were also estimated. Lambs were randomly assigned to one of five diets: i) without additives (Con), ii) with 1% bicarbonate (Bic), iii) with 1% bentonite (Ben), iv) with 33 mg/kg monensin (Mon) and v) with 200 mg/kg fibrolityc enzymes (Enz). In Experiment 2, 120 RambouilletPelibuey intact male lambs ($19.5{\pm}1.5kg\;BW$) were used in a feedlot trial and randomly assigned to four diets: i) without additives (control), ii) with 1% Bic, iii) with 33 mg/kg Mon and iv) with 1% Bic and 33 mg/kg Mon. In Experiment 1, lambs fed diets containing Bic or Mon had significantly higher final weight, DMI, ADG than other lambs. However, apparent DM, OM, CP, NDF and ADF digestibilities and ruminal individual VFA content were similar (p>0.05) among treatments. Conversely, treatmentcollection period interaction was significant for ruminal pH and NH3. In Experiment 2, lambs fed diets containing a Bic and Mon combination had significantly higher final weight, DMI and ADG. It is concluded lambs fed Bic or Mon or Bic and Mon combination had better performance characteristics than lambs on Ben or Enz.

A Study of Activities do Daily Living Performance on Arthritic Elderly Patients (노인의 퇴행성 슬관절염과 일상생활 수행력에 관한 연구)

  • Park Rae-Joon;Park Young-Han
    • The Journal of Korean Physical Therapy
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    • v.14 no.4
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    • pp.183-203
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    • 2002
  • To know the effect of index of social ability (ISA) and function of social life (FSL) with activities of daily living (ADL) and instrumental activities of daily living (IADL), we carried out a study on the elderly with osteoarthritis daily living in Daegu city for 4 months, from April to July 2002. The followings were observed: 1. The score of functional disability of knee, ADL and ISA were better at lower age (p < 0.01). 2. The younger they are, the better FSL they have. The lower age group has good FSL (p < 0.01). 3. Weight affects more to the under 49Kg group than 50-59Kg group and 60-69Kg group in functional disability of knee, while it doesn't make much difference for the other groups (p > 0.05) and other assessment index doesn't show any relationship with weight. 4. Regarding relat ionship with height , taller group has better knee functions: it's proved by post hock examination that the over 160cm group has much higher knee function than the other groups (p < 0.05). In case of ADL, the over 160cm group and under 149cm group were better than 150$\∼$159cm group. (p < 0.01) . 5. ISA was also different depending on height . It was proved that FSL was the better for the over 160cm group than for the others as a result of post hock examination (p < 0.05). 6. In regard of how many months have passed since the attack of functional disability of knee, knee joint function was better for the 12mon$\∼$36mon group than for the others and the score of ADL was lower for the 12mon$\∼$36mon group than for the others (p < 0.01). ISA was lower for the 12mon$\∼$36mon group and the under 6mon group than for the other groups (p < 0.01). FSL was lower for the 12mon$\∼$36mon group and the under 6mon group than the other groups. (p < 0.01). 7. Comparing left knee with right one, the problem with left knee has lower score of functional disability in all items (p < 0.01). 8. Considering friction noise, knee joint function was slightly better for non-friction noise-group, but it's not statistically significant though. ADL and ISA were better for non-friction noise-group (p < 0.01), while no statistical significance was found in FSL, 9. Knee joint function was better for the group who doesn't have limitation on range of knee flexion motion (p,0.05) but no other difference was found in other items from this group. 10. Limitation on range of knee flexion motion has no statistical difference in ADL and FSL. However, ISA was better for the non-limitation group (p < 0.05) . 11. Knee joint function score has high correlation with ISA (r=0.812, p < 0.01) and FSL (r=0.732, p < 0.01) , which shows the better knee joint function, the higher the score. 12. ISA and FSL were highly correlated: the better ISA, the better FSL (r=0.893, p < 0.01), while ADL has no correlation with either knee joint function or ISA or FSL.

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