• Title/Summary/Keyword: Mollusc

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Presence of Proboscipedia and Caudal Gene Homologues in a Bivalve Mollusc

  • Carpintero, Pablo;Pazos, Antonio Juan;Abad, Marcelina;Sanchez, Jose Luis;De La Luz Perez-Paralle, Maria
    • BMB Reports
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    • v.37 no.5
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    • pp.625-628
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    • 2004
  • Homeobox genes encode a family of transcription factors that have essential roles in regulating the development of eukaryotes. Although they have been extensively studied in different phyla, relatively little is known about homeobox-containing genes and their function in molluscs. In this study, we used a polymerase chain reaction to investigate homeobox genes in the bivalve mollusc Pecten maximus. Four different homeobox sequences were identified; two were homologues of the non-Hox cluster gene caudal and the two remaining sequences had a significant homology to the ANT-C gene proboscipedia. These sequences represent the first cad and pb homologues isolated from a member of the class Bivalvia, phylum Mollusca.

The characteristics of black pearl cultured using Pen shell (Atrina pectinata) (키조개(Atrina pectinata)를 이용하여 양식한 흑진주의 특성평가)

  • Lee, Jeong-Im;Kim, Pan-Chae
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.18 no.5
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    • pp.205-210
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    • 2008
  • Pearl is the organic gemstone which does not come from mines but from the biomineralization inside mollusc. Mollusc with nacre on inner surface of the shell is inevitable to make pearl. In this paper we researched and analyzed the pearls cultured using Pen shell (Atrina pectinata) which is not used in pearl farming industry but has potential to make pearls because it has thick and beautiful nacre inside the shell. SEM analysis was conducted to reveal the pattern of nacre on the Atrina pectinata pearl. Specific characteristics as sea-water pearl are detected by further analysis with ED-XRF. Aragonite specific peaks such as $1083cm^{-1}$ and $705cm^{-1}$ were shown by Raman analysis. UV-Vis analysis of Atrina pectinata pearl showed different pattern of spectrum compared with Pinctada margaritifera pearl. The reason for this discrepancy is assumed by the metabolic difference of each species.

Inhibitory Effect of Oyster Conchioloin on Pro-inflammatory Mediator in Lipopolysaccharide;Activated Raw 264.7 Cells (모려로부터 추출된 conchiolin의 LPS로 유도된 RAW 264.7 세포에서의 항염증 효과)

  • Park, Sang-Mi;Zhao, Rong Jie;Lee, Jong-Rok;Lee, Chul-Won;Kim, Hak-Ju;Kwon, Young-Kyu;Kim, Sang-Chan
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.22 no.4
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    • pp.878-883
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    • 2008
  • Conchiolin is a complex protein which is secreted by the mollusc's outer epithelium to be the organic basis of mollusc shell. This study is to investigate a potential anti-inflammatory activity of conchiolin of oyster shell (COS). We tested the effects of COS on the lipopolysaccharide (LPS)-induced production of nitric oxide (NO) and prostaglandin E2 (PGE 2) in a murine macrophage cell line, RAW 264.7. COS inhibited production of NO and PGE2 in a dose dependent manner and also decreased the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$) and interleukin-6 (IL-6). These results suggest that COS can inhibit production of pro-inflammatory mediators and might be a useful source to treat inflammation.

Distribution of Indicator Organisms in Commercial Fish and Shellfish and Influence of Storage Temperature and Period (시판 어패류에서의 오염지표세균의 분포와 저장 온도 및 저장 기간이 오염지표세균에 미치는 영향)

  • 박석기;이용욱;김정현;이강문
    • Journal of Food Hygiene and Safety
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    • v.11 no.1
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    • pp.57-70
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    • 1996
  • This paper intends to investigate commercial fish and shellfish 25 species (fish 8 species, shellfish 7 species, crustacean 3 species, moluse 4 species and echinodermata) for the distribution of sanitary indicator organisms (total viable counts, coliforms, staphylococci, vibrios, and enterococci) and distributional change of indicator organisms accordion to storage temperature and period. The logarithmic mean of total viable counts for total commercial fish and shellfish 25 species was 5.41$\pm$.026 CFU/g, and in accordance with fish and shellfishes, crustacean 6.76$\pm$0.67 CFU/g, shellfish 5.67$\pm$0.56 CFU/g, echinodermata 5.47$\pm$1.50 CFU/g, fish 5.02$\pm$0.38 CFU/g, and mollusc 5.03$\pm$0.65 CFU/g. The logarithmic mean of enterococci was 2.36$\pm$0.37 CFU/g, and in accordane with fish and shellfish, crustacean 3.44$\pm$2.12 CFU/g, shellfish 3.87$\pm$0.45 CFU/g, echinodermata 3.38$\pm$0.0 CFU/g, fish 2.16$\pm$0.41 CFU/g and mollusc 0.01$\pm$0.0 CFU/g. The logarithmic mean of vibrios was 1.60$\pm$1.59 CFU/g, and in accordance with fish and shellfish, crustacean 4.23$\pm$1.11 CFU/g, shellfish 3.58$\pm$0.90 CFU/g, echinodermata 1.64$\pm$0.34 CFU/g, fish 1.79$\pm$0.67 CFU/g and mollusc 1.07$\pm$0.61 CFU/g. The logarithmic mean of staphylococci was 1.60$\pm$1.59 CFU/g, and in accordance with fish and shellfish, shellfish 0.01$\pm$0.00 CFU/g, echinodermata 3.51$\pm$0.60 CFU/g, fish 1.68$\pm$0.64 CFU/g, crustacean 0.34$\pm$0.33 and mollusc 3.90$\pm$0.11 CFU/g. The logarithmic mean of coliformas was 2.52$\pm$0.32 CFU/g, and in accordance with fish and shellfish, echinodermata 3.58$\pm$1.89 CFU/g was highest, shellfish 3.25$\pm$0.30 CFU/g, crustacean 3.23$\pm$0.49 CFU/g, fish 2.18$\pm$0.63 CFU/g, peeled shellfish 1.80$\pm$0.51 CFU/g and mollusc 1.55$\pm$0.95 CFU/g. As the results of research of the change of the contaminate indicator microflora in working with storage period at 1$0^{\circ}C$, 2$0^{\circ}C$ and 3$0^{\circ}C$, total viable counts was increased without storage temperature and enterococci were decreased slowly at 1$0^{\circ}C$, but increased at 20 and 3$0^{\circ}C$. Vibrios were decreased slowly at 1$0^{\circ}C$, decreased at 2$0^{\circ}C$ and 3$0^{\circ}C$in 2 days after increased rapidly. Staphylococci were increased promptly without storage temperature in 2 days, then the total viable counts were maintained. Coliforms were increased at 1$0^{\circ}C$ by 7 days, then decreased or maintained after 14 days, changed at 2$0^{\circ}C$ in accordance with fish species in 2 days, then returned to the initial total viable count, and decreased rapidly at 3$0^{\circ}C$ on 2 days. By the way, there were no difference among the species.

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Projection Pathway of Specific FMRFamide-producing Neurons in Terminal Abdominal Ganglion Innervating Hindgut

  • Na, So-Young;Lee, Bong-Hee
    • Proceedings of the Korean Society of Sericultural Science Conference
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    • 2003.04a
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    • pp.74-74
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    • 2003
  • Tetrapeptide FMRFamide, originally isolated and then characterized from the mollusc (Clam) Macrocallista, had been demonstrated to have action as a cardioexcitatory agent. During the next two decades since its discover, neuropeptide FMRFamide and a large family of related peptides have been shown to exist in the central nervous system and gut of a wide variety of invertebrates and vertebrates including insect species. (omitted)

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불가사리 (Asterina pectinlfera)로부터 항균성 물질의 정제

  • 서정길;김찬희;김은정;고혜진;김인혜;김창훈;박남규
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2001.05a
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    • pp.230-231
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    • 2001
  • 지금까지 많은 항균성 펩타이드가 포유류 (1), 양서류 (2), 곤충 (3) 및 식물 (4)로 부터 단리ㆍ정제되었고, 구조-활성간의 연구가 활발히 진행되고 있지만, 이러한 연구는 육상생물에 제한적으로 수행되어 겼으며 해양생물을 대상으로 한 연구는 극히 제한되어 있는 상태이다. 해양생물로부터 정제된 항균성 펩타이드로서는 Flounder skin 분비물에서 pleurocidin (5), Moses sole fish에서 pardaxins (6), Horseshoe crab에서 Tachyplesin I(7), Tunicates hemocytes에서 Clavanins (8), Mollusc에서 Mytilins (9) 등이 보고되고 있지만 불가사리를 이용한 항균성물질의 연구는 희박한 실정이다. (중략)

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Growth activation of the microalgae Tetraselmis suecica by the aqueous of the seaweed Monostrama nitidium

  • Cho, Ji-Young;Hong, Yong-Ki
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2001.05a
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    • pp.201-202
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    • 2001
  • Mass culture of microalgae as feed for mollusc, crustaceans and fish is an important components of the mariculture industry (Metting Jr., 1996) Growth activator for tetraselmis suecica were screened in methanol and water soluble extracts of several seaweed tissues. Also examined in this study were change in growth rate, biochemical composition, and digestion efficiency, of T.suecica cultured with and without the most effective of these extracts, the water soluble component extracted form M. nitidium. (omitted)

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Molluscan Remains from the Neolithic Shell Middens in the Southern Coast, Korea (우리나라 남해안지역 신석기시대 패총 출토 패류)

  • 안덕임
    • The Korean Journal of Malacology
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    • v.10 no.2
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    • pp.1-9
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    • 1994
  • Molluscs from the Neolithic shell middens (Sugari, Pudjeong, Tongsamdong, Sangnodaedo, Sandeung, Yondaedo, Songdo and Kupyongri) in the southern coast, Korea, consisted of marine, fresh-water and land molluscs. Among these, intertidal species were exploited most abundantly as food resources, indicating a high dependence of intertidal shellfish collection activities. Especially oysters were the most abundant in these sites except Tongsamdong where mussels were predominant, indicating oysters were the most important food resources. Land snails from the sites consisted of woodland and open land species. Woodland species were more abundant than openland species, suggesting that there was a woodland environmint around the sites. Marine and land mollusc species from the sites are common in these rigions today, indicating no large-scale emvironmental changes have occurred since these middens formation period.

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A Comparative Study on the Changing Pattern of Fish and Shellfish Uses in ${\ulcorner}Eum-sik-di-mi-bang{\lrcorner}$ and ${\ulcorner}Gyu-hap-chong-seo{\lrcorner}$ in Sight of the Development of Fishing Technology (어업기술의 발전 측면에서 본 음식디미방과 규합총서 속의 어패류 이용 양상의 비교 연구)

  • Kim, Hee-Sun
    • Journal of the Korean Society of Food Culture
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    • v.19 no.3
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    • pp.273-284
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    • 2004
  • This study is on the changing pattern of fish-and-shellfish uses during the last two thirds of Chosun period on the premise that they are influenced by development of fishing technology. With a literary approach, this paper researches how fish-and-shellfish production and consumption pattern changed, especially in relation to technological development of fisheries. First, we examine into the changes of fishery production. And next, we analyze the kind and cooking method of fish-and-shellfish in [Eum-sik-di-mi-bang](1670) and [Gyu-hap-chong-seo](1815). The analysis of two books revealed that there were significant differences in fish-and-shellfish uses. Because the two books were written with a gap of 145 years and the development of fishing technology and remarkable changes of fishery production affected on the fish-and-shellfish uses. Due to primitive fishing tools and skills, fresh-water and reverse-river-fish and shellfish had been caught in substantial amount until the middle ages of Chosun period. As a result, the availability of seafood were limited extremely even in the upper classes. These situations are evidenced by the analysis of [Eum-sik-di-mi-bang]. Only 12 kinds of fish-and-shellfish are described in [Eum-sik-di-mi-bang]. Most of the sesfoods is mollusc which is easy to catch. As for the salt-water fish, dried cod and dried herring were mentioned. Mullet, the reverse-water-fish, is used most frequently. Only one kind of 'Hoe', which needs extreme freshness, is described. This means that the use of fresh fish-and-shellfish was very limited to some kinds which could be caught near the village. As the netting fishery began to be developed in the 18th century, the production of some salt-water fishes, such as anchovy, shrimp, yellow corvina, pollack, and herring, had increased remarkably to make marine resources more available. Small fish, such as anchovy and shrimp were preserved as 'Jeot-gal' and sold nationwide. Therefore, 'Jeot-gal' and seafood could be used in Kimchi around this time and had a deep influence on the change of Kimchi in taste and nutrition. In [Gyu-hap-chong-seo], 33 kinds of fish-and-shellfish are described. Including cod and herring, 17 kinds of sea water fish and mollusc are mentioned. Some of these are consumed in fresh state, neither as dried nor as salted. Because the merchants promoted the transport of seafoods to other regions according to the growth of commercial economy. As a result the diet of the people could be enriched by the various seafoods.

Cloning and Characterization of endo-β-1,4-glucanase genes from the Midgut of the Earthworm, Eisenia andrei (지렁이 중장에서 발현되는 endo-β-1,4-glucanase 유전자들의 클로닝과 특성에 관한 연구)

  • Lee, Myung-Sik;Park, Sang-Kil;Tak, Eun-Sik;Ahn, Chi-Hyun;Kim, Hye-Ryung;Park, Soon-Cheol
    • Journal of the Korea Organic Resources Recycling Association
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    • v.15 no.3
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    • pp.80-89
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    • 2007
  • Two endogenous endo-${\beta}$-1,4-D-glucanase (EGase, EC 3.2.1.4) cDNAs were cloned from the midgut of the earthworm Eisenia anderi, and named EaEG2 and EaEG3, respectively. A sequence of 1,368 bp was determined and the coding region is composed of 456 amino acid residues including the initiation methionine. The N-terminal region of 20 residues in the deduced sequence was regarded as the signal peptide. These EGases belong to glycosyl hydrolase family 9 (GHF9) and showed high levels of identity(51-55%) with selected termite, cockroache, crayfish and mollusc EGases. The EGases of earthworm consist of three consensus catalytic domains found in most microbial cellulases. A phylogenetic tree was constructed using the deduced amino acid sequence data matched through the BLASTX program and showed that GHF9 families could be divided into five groups of arthropoda, bacteria, plant, annelida and mollusc.

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