BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
권호 표지
DOI QR코드

DOI QR Code

Presence of Proboscipedia and Caudal Gene Homologues in a Bivalve Mollusc

  • Carpintero, Pablo (Laboratorio de Biologia Molecular y del Desarrollo, Departamento de Bioquimica y Biologia Molecular, Instituto de Acuicultura, Universidad de Santiago de Compostela) ;
  • Pazos, Antonio Juan (Laboratorio de Biologia Molecular y del Desarrollo, Departamento de Bioquimica y Biologia Molecular, Instituto de Acuicultura, Universidad de Santiago de Compostela) ;
  • Abad, Marcelina (Laboratorio de Biologia Molecular y del Desarrollo, Departamento de Bioquimica y Biologia Molecular, Instituto de Acuicultura, Universidad de Santiago de Compostela) ;
  • Sanchez, Jose Luis (Laboratorio de Biologia Molecular y del Desarrollo, Departamento de Bioquimica y Biologia Molecular, Instituto de Acuicultura, Universidad de Santiago de Compostela) ;
  • De La Luz Perez-Paralle, Maria (Laboratorio de Biologia Molecular y del Desarrollo, Departamento de Bioquimica y Biologia Molecular, Instituto de Acuicultura, Universidad de Santiago de Compostela)
  • Published : 2004.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Homeobox genes encode a family of transcription factors that have essential roles in regulating the development of eukaryotes. Although they have been extensively studied in different phyla, relatively little is known about homeobox-containing genes and their function in molluscs. In this study, we used a polymerase chain reaction to investigate homeobox genes in the bivalve mollusc Pecten maximus. Four different homeobox sequences were identified; two were homologues of the non-Hox cluster gene caudal and the two remaining sequences had a significant homology to the ANT-C gene proboscipedia. These sequences represent the first cad and pb homologues isolated from a member of the class Bivalvia, phylum Mollusca.

Keywords

References

  1. Abzhanov, A., Holtzman, S. and Kaufman, T. C. (2001) The Drosophila proboscis is specified by two Hox genes, proboscipedia and Sex combs reduced, via repression of leg and antennal appendage genes. Development 128, 2803-2814.
  2. Ausubel, F., Brent R., Kingston R. E., Moore, D. D., Seidman, J. G., Smith, J. A. and Struhl, K. (1992) Short protocols in molecular biology, 3rd ed., Wiley and Sons, Inc., USA.
  3. Burglin, T. R. (1994) A comprehensive classification of homeobox genes; in Guidebook to the homeobox genes, Duboule, D. (ed.), pp. 25-71, Oxford University Press, Oxford, England.
  4. Callaerts, P., Lee, P. N., Hartmann, B., Farfan, C., Choy, D. W. Y., Ikeo, K., Fischbach, K. -F., Gehring, W. J. and Gert de Couet, H. (2002) Hox genes on the sepiolid squid Euprymna scolopes: Implications for the evolution of complex body plans. Proc. Natl. Acad. Sci. USA 99, 2088-2093. https://doi.org/10.1073/pnas.042683899
  5. Carroll, S. B., Grenier, J. and Weatherbee, S. D. (2001) From DNA to diversity: molecular genetics and the evolution of animal design. Blackwell Science, Oxford, England.
  6. Dearden, P. K. and Akam, M. (2001) Early embryo patterning in the grasshopper, Schistocerca gregaria: wingless, decapentaplegic and caudal expression. Development 128, 3435-3444.
  7. Dearolf, C. R., Topol, J. and Parker, C. S. (1989) The caudal gene product is a direct activator of fushi tarazu transcription during Drosophila embryogenesis. Nature 341, 340-343. https://doi.org/10.1038/341340a0
  8. DeCamillis, M. A., Lewis, D. L., Brown, S. J., Beeman, R. W. and Denell, R. E. (2001) Interactions of the Tribolium Sex combs reduced and proboscipedia orthologs in embryonic labial development. Genetics 159, 1643-1648.
  9. Degnan, B. M. and Morse, D. E. (1993) Identification of eight homeobox-containing transcripts expressed during larval development and at metamorphosis in the gastropod mollusc Haliotis rufescens. Mol. Mar. Biol. Biotechnol. 2, 1-9.
  10. Diederich, R. J., Pattatucci, A. M. and Kaufman, T. C. (1991) Developmental and evolutionary implications of labial, deformed and engrailed expression in the Drosophila head. Development 113, 273-281.
  11. Epstein, M., Pillemer, G., Yelin, R., Yisraeli, J. K. and Fainsod, A. (1997) Patterning of the embryo along the anterior-posterior axis: the role of the caudal genes. Development 124, 3805-3814.
  12. Gehring, W. J. (1987) Homeo boxes in the study of development. Science 236, 1245-1252. https://doi.org/10.1126/science.2884726
  13. Hinman, V. F. and Degnan, B. M. (2002) Mox homeobox expression in muscle lineage of the gastropod Haliotis asinina: evidence for a conserved role in bilaterian myogenesis. Dev. Genes Evol. 212, 141-144. https://doi.org/10.1007/s00427-002-0223-6
  14. Lee, S. E., Gates, R. D. and Jacobs, D. K. (2001) The isolation of a Distal-less gene fragment from two molluscs. Dev. Genes Evol. 211, 506-508. https://doi.org/10.1007/s00427-001-0184-1
  15. O'Brien, E. K. and Degnan, B. M. (2000) Expression of POU, Sox and Pax genes in the brain ganglia of the tropical abalone Haliotis asinina. Mar. Biotechnol. 2, 545-557. https://doi.org/10.1007/s101260000039
  16. O'Brien, E. K. and Degnan, B. M. (2002) Developmental expression of a class IV POU gene in the gastropod Haliotis asinina supports a conserved role in sensory cell development in bilaterians. Dev. Genes Evol. 212, 394-398. https://doi.org/10.1007/s00427-002-0256-x
  17. Percival-Smith, A., Weber, J., Gilfoyle, E. and Wilson, P. (1997) Genetic characterization of the role of two Hox proteins, Proboscipedia and Sex combs reduced, in determination of adult antennal, tarsal, maxillary palp and proboscis identities in Drosophila melanogaster. Development 124, 5049-5062.
  18. Rush, D. B. and Kaufman, T. C. (2000) Regulation of proboscipedia in Drosophila by homeotic selector genes. Genetics 156, 183-194.
  19. Saitou, N. and Nei, M. (1987) The neighbor-joining method: a new method for reconstructing phylogenetics trees. Mol. Biol. Evol. 4, 406-425.
  20. Sambrook, J., Fritsch, E. F. and Maniatis, T. (eds.) (1989) Molecular Cloning: A Laboratory Manual 2nd ed., Cold Spring Harbor Laboratory Press, New York, USA.
  21. Wilbur, K. M. (1983) The Mollusca. Development. Academic Press, New York, USA.
  22. Wray, C. G., Jacobs, D. K., Kostriken, R., Vogler, A. P., Baker, R. and DeSalle, R. (1995) Homologues of the engrailed gene from five molluscan classes. FEBS Lett. 365, 71-74. https://doi.org/10.1016/0014-5793(95)00372-G
  23. Zaneth, M. E., Chan, R. L., Godoy, A. V., Gonzalez, D. H. and Casalongue, C. A. (2004) Homeodomain-leucine zipper proteins interact with a plant homologue of the transcriptional co-activator multiprotein bridging factor 1. J. Biochem. Mol. Biol. 37, 320-324. https://doi.org/10.5483/BMBRep.2004.37.3.320

Cited by

  1. First evidence of the presence ofNK2andTlxgenes in bivalve molluscs vol.91, pp.5, 2013, https://doi.org/10.1139/cjz-2012-0296
  2. The HOX Gene Cluster in the Bivalve Mollusc Mytilus galloprovincialis vol.43, pp.7-8, 2005, https://doi.org/10.1007/s10528-005-6780-4
  3. Conservation of Gbx genes from EHG homeobox in bivalve molluscs vol.63, pp.1, 2012, https://doi.org/10.1016/j.ympev.2011.12.023
  4. Hox, Parahox, Ehgbox, and NK Genes in Bivalve Molluscs: Evolutionary Implications vol.35, pp.1, 2016, https://doi.org/10.2983/035.035.0119
  5. An Overview of Hox Genes in Lophotrochozoa: Evolution and Functionality vol.4, pp.1, 2016, https://doi.org/10.3390/jdb4010012
  6. Unexpected variation of Hox genes’ homeodomains in cephalopods vol.40, pp.3, 2006, https://doi.org/10.1016/j.ympev.2006.04.004
  7. HoxandParaHoxgenes: A review on molluscs vol.52, pp.12, 2014, https://doi.org/10.1002/dvg.22839