• Bulletin of the Korean Chemical Society
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• v.28 no.5
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• pp.847-850
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• 2007

• Macromolecular Research
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• v.8 no.5
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• pp.243-245
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• 2000

can be determined from the number and weight average molecular weight. When the value of the exponent, a, appearing in the relation between the intrinsic viscosity and the viscosity average molecular weight, is in the range from 0.6 to 0.8, as is the case for the most polymers, the viscosity average molecular weight is much more close to the weight average molecular weight than to the number average molecular weight.

• Polymer(Korea)
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• v.38 no.6
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• pp.735-743
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• 2014

The rheological measurement of polypropylene (PP) has been performed using a rheometer, an intrinsic viscometer, and an MI machine to predict the molecular weight and the molecular weight distribution. Also, GPC has been used for the determination of the molecular structure. The distribution broadness parameter using modified Carreau model has been used to make the correlation between the rheological parameter and the molecular structure instead of the rheological PI (polydispersity index) which is determined from the cross of modulus from the dynamic oscillatory measurement. Even though the rheological PI is useful to determine the molecular weight distribution of the PP using controlled rheology, which has narrow and uniform molecular weight distribution, but not suitable to determine the molecular weight distribution of the PP made from direct polymerization which has broad and various molecular weight distribution. However the distribution broadness parameter which determined from the index of the shear thinning of the PP melt well predicts the molecular weight distribution of PP.

• Bulletin of the Korean Chemical Society
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• v.16 no.7
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• pp.640-643
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• 1995

The internal standard method, which is widely used in chromatographic techniques, is applied to the size exclusion chromatography for the on-line determination of dn/dc value of the sample. This method is found to provide the dn/dc value with suitable accuracy in determining the absolute molecular weight and the molecular weight distribution of the polymers when a light scattering detector is used.

• Bulletin of the Korean Chemical Society
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• v.27 no.3
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• pp.413-418
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• 2006

Sodium hyaluronate (NaHA), water soluble polymer having ultra-high molecular weight, is characterized by using on-line frit inlet asymmetrical flow field-flow fractionation (FI-AFlFFF) and multiangle light scattering (MALS). This study demonstrates the capability of power programming FI-AFlFFF for the separation of NaHA and the applicability of FI-AFlFFF with MALS for the characterization of molecular weight distribution and their structural information. Since sample injection and relaxation in FI-AFlFFF are achieved by using hydrodynamic relaxation, separation of high molecular weight polymers can be achieved smoothly without halting the separation flow. Experiments are carried out with the two different NaHA products (a raw NaHA sample and a thermally degraded NaHA product) and molecular weight distribution and conformations in solution are determined. Influence of sample filtration on the change of molecular weight distribution is also discussed.

• Analytical Science and Technology
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• v.20 no.3
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• pp.255-260
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• 2007

A new method for the simultaneous determination of low molecular weight amines and quaternary ammonium ions based on the separation by IC with a suppressor and the detection by MS with ESI has been developed. The method has been applied to the analysis of a mixture containing tetramethylammonium ion, tetraethylammonium ion, tetrapropylammonium ion, triethanolamine, trimethylamine and triethylamine. The constituents were separated by isocratic elution using an IonPac CS17 column, a cation-exchange column, and detected by conductivity and mass spectrometry. The newly developed method for the six components demonstrated that the repeatability in terms of relative standard deviation for three measurements was in the range of 0.1-0.5 %. The detection limits were between 0.2 and $0.9{\mu}g/mL$ by the IC/ESI-MS.

• Analytical Science and Technology
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• v.8 no.4
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• pp.465-468
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• 1995

Matrix assisted laser desorption ionization in mass spectrometry is a fast and accurate method to determine the molecular weight of natural and synthetic polymers. Unknown peptides such as elastase inhibitor and $\small{D}$-hydantoinase were analyzed using sinapinic acid as matrix and their molecular weights were compared with the results from protein sequencer and gel filtration chomatography, respectively. Synthetic polymers such as polyethyleneglycol, polypropyleneglycol, polydimethylsiloxane, and polystyrene were analyzed using matrices such as 2,5-dihydroxybenzoic acid, 4-hdroxyazobenzenecarboxylic acid, and 2-nitrophenyl octyl ether. Average molecular weights of polystyrene were compared with molecular weights by gel permeation chromatography.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.30 no.4
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• pp.59-68
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• 1998

This study was to investigate the effect of chitosan molecular weight, its charge density, and its surface coating treatment on the antibacterial properties of paper. For this study, E.coil was used for antibacterial experiment. Results obtained were as follows : 1. The antibacterial properties of chitosan was significant on the surface-treated sheet. 2. Antibacterial property surface treatment was appeared to be effective when film was formed on the paper surface. 3. The antibacterial properties of chitosan-treated paper was dependent on the amount and the molecular weight of chitosan used. The lower the molecular weight of the chitosan down to 30,000 the better the antibacterial properties in this experiment. 4. Determination of the degree of chitosan-deacetylation by colloidal titration method was consistent with the more complicated and conventional FT-IR method.

• Journal of the Korean Chemical Society
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• v.21 no.3
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• pp.215-218
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• 1977

We have investigated for amino acid composition, molecular weight and terminal amino acid of the main gliadin protein which was purified by Sephadex column. The results were obtained as follows: 1. The highest content of amino acid was glutamic acid for the main gliadin protein. 2. The molecular weight was estimated 60,200 ${\pm}$200 for the main gliadin protein. 3. Identified N-terminal and C-terminal amino acid of the main gliadin protein were phenylalanine and methionine, respectively.

• Proceedings of the Botanical Society of Korea Conference
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• 1999.04a
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• pp.18-18
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• 1999

We characterized a cDNA clone for a low molecular weight heat-shock protein (LMW HSP) from tobacco named TLHS-l. Nucleotide sequence determination of TLHS-1 identified an open reading frame for 159 amino acids. To the upstream of the open reading frame, a sequence of 124 nucleotides was determined. To the 3' downstream of the open reading frame, 212 nucleotides were identified which carried poly(A)-tail. Comparison of the open reading frame and hydropathy plot of TLHS-1 with the previously reported class I LMW HSPs showed high identity which classified TLHS-1 as a class I LMW HSP cDNA clone. We proposed that there are six consensus regions in class I LMW HSPs. RNA blot hybridization for TLHS-1 showed a typical expression pattern of heat-shock-inducible gene from three common tobacco cultivars. The open reading frame of TLHS-1 was overexpressed in Escherichia coli. TLHS-1 protein confers thermal protection of other proteins in vitro and in vivo. Thermal induced aggregation of citrate synthase was reduced by purified TLHS-1 protein, and thermal death rate at $50^{\circ}C$ was reduced in E. coli expressing TLHS-l. From these data, we can expect that TLHS-1 acts as a molecular chaperone.perone.