• Title/Summary/Keyword: Molecular Characteristics

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Galactomyces pseudocandidus (Dipodascaceae): An Unrecorded Yeast-like Fungus Isolated from the Digestive Tract of Procambarus clarkii

  • Eo, Ju-Kyeong;Park, Eunsu;Kim, Suhwan;Choe, Han-Na
    • The Korean Journal of Mycology
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    • v.49 no.3
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    • pp.393-397
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    • 2021
  • The unrecorded fungus Galactomyces pseudocandidus de Hoog & M.T. Sm. was isolated and cultured from an introduced species, Procambarus clarkii, in Korea. Two related species, Galactomyces citri-aurantii and Geotrichum candidum, have been reported, but no other Galactomyces spp. have been found in Korea. In this study, we isolated G. pseudocandidus from the digestive tract of American crayfish using RGY (river water-glucose-yeast) medium and then compared the morphological and molecular characteristics of this species with those of related species. As a result, we confirmed that G. pseudocandidus is an unrecorded fungus in Korea, so we described and reported the cultural characteristics and microstructures, such as conidia.

Acaulospora jejuensis, a New Species in Glomeromycota from Korea

  • Park, Hyeok;Ka, Kang-Hyeon;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.49 no.4
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    • pp.425-431
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    • 2021
  • New species of arbuscular mycorrhizal fungi (Glomeromycota), Acaulospora jejuensis, was isolated from rhizosphere soils of Miscanthus sinensis in the grassland in Jeju Island of Korea. The species was identified using the morphological characteristics of the spores and the molecular phylogenetic analysis using partial DNA sequences from small subunit rDNA (SSU), internal transcribed spacer (ITS), and large subunit rDNA (LSU). Phylogenetic analysis placed the fungal species in a distinct clade within genus Acaulospora. Also, the species exhibited the morphological characteristics distinct from the other members of the genus. Therefore, Acaulospora jejuensis was described as a novel species from Korea.

Seven Previously Unrecorded Fungal Species Isolated from Freshwater Ecosystems in Korea

  • Goh, Jaeduk;Mun, Hye Yeon;Oh, Yoosun
    • The Korean Journal of Mycology
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    • v.49 no.2
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    • pp.183-197
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    • 2021
  • Various freshwater ecosystems, such as streams, lakes and rivers, provide a dynamic habitat for fungi. In this study, we isolated several fungal strains from freshwater sediment and plant litter. The strains were identified using molecular phylogenetic analyses of rDNA and beta tubulin (TUB) sequences. Morphological characteristics of the fungi were also investigated using microscopy and culture characteristics of the fungi grown on several media. We identified seven species previously unrecorded in Korea, Nothophoma spiraeae, Westerdykella aquatica, W. aurantiaca, W. dispersa, Chrysosporium sanyaense, C.pseudomerdarium and Taeniolella phialosperma.

Four Unreported Endophytic Fungi Isolated from Roots of Quercus spp. in Korea

  • Choi, Jae-Wook;Gwon, Ju-Hui;Park, Hyeok;Lee, Jong-Chul;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.49 no.3
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    • pp.325-335
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    • 2021
  • We isolated endophytic fungi from the roots of Quercus spp. in Korea. The isolates were identified based on morphological and molecular characteristics from the nucleotide sequences of the internal transcribed spacer and large subunit of rDNA regions. We confirmed that the four species we isolated - Oidiodendron citrinum, Acremonium crotocinigenum, Mammaria echinobotryoides, and Pleotrichocladium opacum - have not been recorded previously in Korea. Herein, we present their morphological characteristics and the result of phylogenetic analysis.

An Unrecorded Genus Lambertella Höhn. (Rutstroemiaceae) and Its Unrecorded Species in Korea

  • Choi, Jae-Wook;Park, Eunsu;Eo, Ju-Kyeong
    • The Korean Journal of Mycology
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    • v.49 no.1
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    • pp.127-131
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    • 2021
  • Lambertella corni-maris Höhn., an endophytic fungus that is an unrecorded species in Korea, was isolated and cultured from alpine coniferous Korean fir. The Lambertella sp. was found in Korean pine in Yeongwol-gun but no Lambertella spp. were recorded elsewhere in Korea. In this study, the morphological characteristics and molecular barcode sequence of L. cornimaris were compared to its related species. We tried to explain the cultural characteristics and microstructures, such as the conidia of L. corni-maris, compared to closely related taxa and present it.

Morphological and Multigene Sequence Characteristics of Talaromyces variabilis Isolated from Soil in Korea

  • Adhikari, Mahesh;Kim, Sang Woo;Lee, Hyang Burm;Lee, Youn Su
    • The Korean Journal of Mycology
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    • v.49 no.1
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    • pp.11-19
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    • 2021
  • In 2017, Talaromyces variabilis was isolated during a survey of fungal diversity in field soils in Korea. This isolate was described based on its morphological and molecular characteristics and it was identified molecularly using the partial 18S-ITS1-5.8S-ITS2-28S rDNA region and calmodulin (CaM)-encoding gene sequence data. Thus, this study reported morphological and multigene sequence characterization of T. variabilis.

Comparative Studies of Molecular Mechanisms of Dopamine D2 and D3 Receptors for the Activation of Extracellular Signal Regulated Kinase 1/2 in HEK-293 cells

  • Cheong, Da-Woon;Cho, Choon-Sil;Kim, Hyun-Ju;Kim, Kyeong-Man
    • Proceedings of the PSK Conference
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    • 2002.10a
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    • pp.270.2-270.2
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    • 2002
  • Dopamine D2 and D3 receptors (D2R and D3R) belong to pharmacological D2R family and share similar structural and functional characteristics. Elucidation of their differential functional characteristics is important for understanding their roles in brain. ERK1/2 was chosen as an example of signaling component of D2R and D3R and systemic studies were conducted to understand the regulatory mechanisms on ERK1/2 activation. (omitted)

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Penicillium mexicanum: An Unrecorded Fungal Species Isolated from Air Samples Collected in Korea

  • Jung-Min Lee;Jae-Eui Cha;Young-Sil Yoon;Ahn-Heum Eom
    • The Korean Journal of Mycology
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    • v.51 no.2
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    • pp.127-133
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    • 2023
  • We report the first discovery of Penicillium mexicanum in Korea. Fungal strains were isolated from air samples collected in Taean-gun, Chungcheongnam-do, Korea. The strain was identified based on its morphological characteristics, as well as molecular phylogenetic analysis of the internal transcribed spacer (ITS), β-tubulin (BenA), and calmodulin (CaM) regions. This strain exhibited a high sequence similarity to the reference sequences of P. mexicanum. These findings enhance our understanding of fungal biodiversity in Korea and underscore the importance of continuous monitoring of fungal species.

Clonostachys divergens and Chrysosporium merdarium: Two New Records from Soil in Korea

  • Whee Phaund;Ung Somaly;Kallol Das;Seung-Yeol Lee;Hee-Young Jung
    • The Korean Journal of Mycology
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    • v.51 no.2
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    • pp.91-100
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    • 2023
  • During an investigation of micro-fungi in soil, two fungal isolates belonging to the phylum Ascomycota, namely KNUF-20-NI011 and KNUF-20-NI006, were collected from Gyeongbuk Province and Dokdo Island in Korea and identified as Clonostachys divergens and Chrysosporium merdarium, respectively. The fungal isolates were confirmed through molecular phylogenetic analyses of the internal transcribed spacer regions, 28S rDNA large subunit, and β-tubulin sequences. Cultural and morphological characteristics were observed and determined using different media. These species were identified based on phylogenetic relationships along with their cultural and morphological characteristics. To our knowledge, this is the first report on Clonostachys divergens and Chrysosporium merdarium in Korea.

Three Unreported Fungi Isolated From Reservoirs in Korea: Mortierella biramosa, Paraphoma radicina, and Sordaria macrospora

  • Bora Nam;Hyang Burm Lee;Young-Joon Choi
    • The Korean Journal of Mycology
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    • v.50 no.2
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    • pp.103-113
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    • 2022
  • Freshwater ecosystems have a large reserve of latent biological resources that play an essential ecological role, and have significant economic and social value. Fungi in freshwater are prospective materials that can be used in the food, medicine, and biomass energy fields. In this study, three promising fungal species were isolated from freshwater ecosystems in Korea. These isolates were identified as Mortierella biramosa, Paraphoma radicina, and Sordaria macrospora, based on their cultural and morphological characteristics, as well as molecular phylogenetic analyses. These species were previously unknown in Korea. The finding allows us to explore its physiological and biochemical characteristics in more detail and use them as biological resources.