• Title/Summary/Keyword: Molecular Characteristics

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Morphological and molecular characterization of the genus Coolia (Dinophyceae) from Bahía de La Paz, southwest Gulf of California

  • Morquecho, Lourdes;Garate-Lizarraga, Ismael;Gu, Haifeng
    • ALGAE
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    • v.37 no.3
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    • pp.185-204
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    • 2022
  • The genus Coolia A. Meunier 1919 has a global distribution and is a common member of epiphytic dinoflagellate assemblages in neritic ecosystems. Coolia monotis is the type species of the genus and was the only known species for 76 years. Over the past few decades, molecular characterization has unveiled two species complexes that group morphologically very similar species, so their limits are often unclear. To provide new knowledge on the biogeography and species composition of the genus Coolia, 16 strains were isolated from Bahía de La Paz, Gulf of California. The species were identified by applying morphological and molecular approaches. The morphometric characteristics of all isolated Coolia species were consistent with the original taxa descriptions. Phylogenetic analyses (large subunit [LSU] rDNA D1 / D2 and internal transcribed spacer [ITS] 1 / 5.8S / ITS2) revealed a species assemblage comprising Coolia malayensis, C. palmyrensis, C. tropicalis, and the C. cf. canariensis lineage. This is the first report of Coolia palmyrensis and C. cf. canariensis in Mexico and C. tropicalis in the Gulf of California. Our results strengthen the biogeographical understanding of these potentially harmful epiphytic dinoflagellate species.

Morphological and Molecular Identification of Two Macrourid Species (Gadiformes) Collected by the Korean Observer from the Southern Ocean (남극해에서 한국 옵서버에 의해 채집된 민태과(대구목) 어류 2종의 형태 및 분자동정)

  • Seo, Min-Ju;Kim, Jin-Koo;Chung, Sangdeok
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.55 no.6
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    • pp.967-972
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    • 2022
  • We investigated the molecular and morphological traits of 338 individual macrourids collected from the Southern Ocean (FAO area number, 88.1 and 88.3) between 2021 and 2022 by Korean bottom trawls. We first identified them as Macrourus caml and Macrourus whitsoni based on morphological traits, such as the number of pelvic fin rays (PF) and the rows of lower jaw teeth (LJT). However four individuals showed uncategorizable morphological characteristics such as PF and LJT numbers that overlapped between the two species. Subsequently, we obtained and analyzed 509 bp of the mtDNA COI sequences of 49 individuals, including the four unidentified individuals, and found only one single nucleotide polymorphism (SNP) that distinguished the two species. Finally, using our molecular identification key, we confirmed that each two individuals were misidentified as M. whitsoni and M. caml reversely. Our results suggest that the number of PF and LJT should be investigated together to accurately identify the two species.

IGRINS NIR Spectroscopy of Diffuse Sources around MWC 1080

  • Kim, Il-Joong;Oh, Heeyoung;Jeong, Woong-Seob;Lee, Jae-Joon
    • The Bulletin of The Korean Astronomical Society
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    • v.44 no.2
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    • pp.48.1-48.1
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    • 2019
  • We found a diffuse Hα feature with a large size of ~2' around a Herbig star, MWC 1080. It shows a strong correlation with the elongated outflow cavity centered on the star. To investigate the diffuse Hα source and the molecular cavity in detail, we carried out the high-resolution NIR spectroscopy using IGRINS. We detected six hydrogen Brackett line series, seven H2 lines, and an [Fe II] forbidden line. With the obtained spatial, kinematic, and line ratio results, we discuss the characteristics of the central MWC 1080A, the NE outflow cavity, and the SE molecular cloud regions separately. Most of the bright Brγ sources around MWC 1080A were found to be reflection nebulae, but a point-like Brγ source close to another young star, MWC 1080E, was identified as a distinct source due to MWC 1080E itself. The narrow components of the H2 lines observed around MWC 1080A were found to trace PDRs located on the wall of the main outflow cavity. Based on the shock-excited H2 and [Fe II] lines detected just inside a bow-shock shape Hα feature, we suggest that it represents the actual shock at the head of the NE outflow from MWC 1080A. Also, we newly detected the shock-excited H2 and [Fe II] lines with highly blueshifted velocities in the SE molecular cloud region. They could be related to unrevealed outflows from other young stars existing around MWC 1080A.

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Terahertz Spectroscopy and Molecular Dynamics Simulation of Five Citrates

  • Siyu Qian;Bo Peng;Boyan Zhang;Jingyi Shu;Zhuang Peng;Bo Su;Cunlin Zhang
    • Current Optics and Photonics
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    • v.8 no.1
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    • pp.86-96
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    • 2024
  • This research investigation employs a terahertz (THz) time-domain spectroscopy system to study the terahertz spectral characteristics of five different citrates in both solution and solid state. The citrates under examination are lithium citrate, monosodium citrate, disodium citrate, trisodium citrate, and potassium citrate. The results show that the THz absorption coefficients of the first four citrate solutions exhibit a decreasing trend with increasing concentration. However, the potassium citrate solution shows an opposite phenomenon. At the same time, the absorption coefficients of lithium citrate, trisodium citrate, and potassium citrate solutions are compared at the same concentration. The results indicate that the absorption coefficient of citrate solution increases in proportion to the increase of metal cation radius, which is explained from the perspective of the influence of metal cations on hydrogen bonds. In addition, we also study the absorption peaks of solid citrates, and characterize the formation mechanism of the absorption peaks by molecular dynamics simulations. This methodology can be further extended to the study of multitudinous salts, presenting theoretical foundations for the detection in food and medicine industries.

Variation of Insulation Characteristics due to ${\gamma}$-ray Irradiation on LDPE Contained Treeing Inhibitors (트리억제제가 첨가된 LDPE의 방사선 조사에 따른 절연특성 변화)

  • Ryu, Boo-Hyung
    • Journal of the Korean Society of Safety
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    • v.27 no.2
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    • pp.13-19
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    • 2012
  • The effectiveness of treeing resistance in low density polyethylene containing azocompounds were investigated under radiation environments. 4-(4-nitrophenylazo)-1-naphthol and 4-(4-nitrophenylazo) resorcinol were selected for treeing inhibitors. LDPE containing the additives were crosslinked with dicumyl peroxide, irradiated by $Co^{60}$ ${\gamma}$-ray in the presence of air at room temperature. Treeing inception voltage, AC breakdown strength measurements were carried out for electrical insulation characteristics, thermoluminescence and gel contents were analyzed as various irradiation doses also. LDPE with treeing inhibitors showed much better insulation characteristics than pure LDPE. It is interpreted that molecular structures, polar groups and the type of radicals connected on the benzene ring of the additives affected electrical characteristics. From the results, 4-(4-nitrophenylazo) resorcinol was the most effective upon the best treeing and radiation resistance.

Study on Boundary Lubrication in the Sliding Bearing System under High Load and Speed (고하중과 고속 미끄럼 베어링 시스템의 경계윤활에 대한 연구)

  • 장시열
    • Tribology and Lubricants
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    • v.15 no.3
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    • pp.248-256
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    • 1999
  • Many tribological components in automobile engine undergo high load and sliding speed with thin film thickness. The lubrication characteristics of the components are regarded as ether hydrodynamic lubrication or boundary lubrication, whereas in a working cycle they actually have both characteristics. Many modem engine lubricants have various additives for better performance which make boundary film formation even under hydrodynamic lubrication regime. Conventional Reynolds equation with the viewpoints of continuum mechanics concerns only bulk viscosity of lubricant, which means that its simulation does not give insights on boundary lubrication characteristics. However, many additives of modern engine lubricant provide mixed modes of boundary lubrication characteristics and hydrodynamic lubrication. Especially, high molecular weight polymeric viscosity index improvers form boundary film on the solid surface and cause non-Newtonian fluid effect of shear thinning. This study has performed the investigation about journal bearing system with the mixed concepts of boundary lubrication and hydrodynamic lubrication which happen concurrently in many engine components under the condition of viscosity index improver added.

Electrical Characteristics of n-GaN Schottky Diode fabricated by using Electrochemical Metallization (Electrochemical Metallization방법을 이용한 GaN Schottky Diode의 제작과 전기적 특성 향상 및 분석)

  • ;Daejun Fu
    • Proceedings of the IEEK Conference
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    • 2001.06b
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    • pp.205-208
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    • 2001
  • Schottky barrier diodes are fabricated on a intrinsic GaN(4${\mu}{\textrm}{m}$) epitaxial structure grown by rf plasma molecular beam epitaxy (MBE) on sapphire substrates. First, We make Ohmic electrodes (Ti/Al/Ti/Au) by evaporator. Next, we contact RuO$_2$ by dipping in the solution (RuCl$_3$.HClO$_4$), and then we deposit Ni/Au on the surface of RuO$_2$ by evaporator. We study the electrical characteristics of GaN Schottky barrier diodes made by these methods. Measurements are C-V, I-V, SEM, EDX, and XRD for the characteristics of devices. Thickness of RuO$_2$ layer depends on supplied voltage and dipping time. Device of thinner RuO$_2$ layer have a good Schottky characteristics compare with device of thicker RuO$_2$ layer

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A comparative study on thermal, mechanical and dielectric characteristics of low density polyethylene crosslinked by radiation and chemical methodes (화학적방법과 방사선으로 가교된 저밀도 폴리에티렌의 열적 기계적 및 유전적 특성의 비교연구)

  • 김봉흡;강도열;김정수
    • 전기의세계
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    • v.25 no.2
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    • pp.100-106
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    • 1976
  • A comparative study on thermal, static mechanical and dielectric characteristics were made over a temperature range of ca.20.deg.C to 320.deg.C and a frequency range of KHZ to MHZ band on low density polyethylene specimens crosslinked, respectively, by radiation and chemical method. The thermal property of both specimens shows that softening point appears to unchange by crosslinking, however, melting and liquidizing temperatures attain rapid increase at the imitiation of crosslinking. Mechanical properties show little difference to both specimens crosslinked by different method, further the behaviors were discussed in connection with the relaxation of molecular segments in amorphous phase. Dose dependent dielectric characteristics observed at ambient temperature under several fixed frequencies exhibit extremities at ca. 20 Mrad and the behaviors also were interpreted qualitatively by taking into consideration of dipole concentration change in amorphous phase together with the role of specimen geometry to the depth of oxidative layer. Observing frequency dependent dielectric characteristics, it was also proved that ionic conduction loss is appreciably greater in the specimen prepared by chemical method than that by radiation.

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Effects of carbonation on hydration characteristics of ordinary Portland cement at pre-curing condition

  • Kim, Gwang Mok
    • Journal of Urban Science
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    • v.11 no.1
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    • pp.21-28
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    • 2022
  • Raman spectroscopy is effective to investigate functional groups via molecular vibration. The technique offers the structural information of compounds including subtle changes in the chemical composition of local atomic coordination without critical damage. Thus, the effect of carbonation on the hydration characteristics of Portland cement under pre-curing conditions for carbonation was investigated via Raman spectroscopy in the present study. Gaseous CO2 was injected within 60 seconds, and the reaction time was varied from 0 minute to 90 minutes. The test results indicated that the Ca/Si ratio of C-S-H reduced immediately after mixing and then the C-S-H with a relatively high Ca/Si ratio coexisted as the reaction time increased. The calcium carbonates formed in the present study included calcite and amorphous calcium carbonates. The test results via Raman spectroscopy provide valuable information about the carbonation characteristics of OPC under pre-curing conditions for carbonation.

Phenotypic and molecular characteristics of second clone (T0V2) plants of the LeLs-antisense gene-transgenic chrysanthemum line exhibiting non-branching (무측지성 국화 형질전환 계통 영양번식 제2세대의 형태적 및 분자생물학적 특성)

  • Lee, Su Young;Kim, Jeong-Ho;Cheon, Kyeong-Seong;Lee, Eun Kyung;Kim, Won Hee;Kwon, O Hyeon;Lee, Hye Jin
    • Journal of Plant Biotechnology
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    • v.40 no.4
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    • pp.192-197
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    • 2013
  • This study examined the phenotypic and molecular characteristics of the $2^{nd}$ clone ($T_0V_2$) plants of LeLs-antisense gene-transgenic chrysanthemum line (LeLs80) that exhibited non-branching, proving the relevance of these characteristics as a factor for use in environmental risk assessment. Results of the Southern blot analysis showed that three copies of the LeLs-antisense gene were introduced into the transgenic line, and northern analysis showed that the transcripted gene was normally expressed in the transgenic line. A flanking T-DNA sequencing method was used to determine that sequences of 184 and 464 bps flanked the LeLs-antisense gene in the transgenic line. These sequences, respectively, matched the 35S promoter for expression of the npt II gene and the NOS terminator for expression of the LeLs-antisense gene within the pCAMBIA 2300 vector.