• 제목/요약/키워드: Molecular Characteristics

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First Report of Sarocladium strictum Causing Sheath Rot of Rice (Oryza sativa) in Korea

  • Berrick Ochieng;Sung Kee Hong;Oliul Hassan;Hyunjoo Ryu
    • The Korean Journal of Mycology
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    • v.52 no.1
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    • pp.19-24
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    • 2024
  • Rice sheath rot causes yield losses of up to 85% in rice-growing areas worldwide. In September 2023, the disease was detected in rice fields at the National Institute of Agricultural Sciences in Wanju, Korea. Two isolates (FD00214 and FD00215) were collected from symptomatic rice sheaths and identified as Sarocladium strictum based on their morphological characteristics and molecular sequencing of two genomic regions: the internal transcribed spacer and actin. Pathogenicity tests confirmed that S. strictum were the cause of the disease. To our knowledge, this is the first report of rice sheath rot caused by S. strictum in South Korea.

Two New Records of Endophytic Fungi Isolated from Lindera obtusiloba in Korea: Colletotrichum citricola and Valsa ceratophora

  • Jae-Eui Cha;Yun-Jeong Kim;Ji-Won Kim;Ahn-Heum Eom
    • The Korean Journal of Mycology
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    • v.52 no.2
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    • pp.135-143
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    • 2024
  • Lindera obtusiloba Blume, a shrub prevalent in the understory vegetation of the deciduous forests in East Asia and Korea, is known for its pharmacological properties, including antioxidant, anti-allergic, and anti-inflammatory activities. Endophytic fungi were isolated from surface-sterilized twigs and leaves of L. obtusiloba collected from Samcheok-si and Jecheon-si in Korea. The fungal strains were identified based on their morphological characteristics and molecular analyses using DNA markers such as internal transcribed spacer, large subunit rDNA, β-tubulin, chitin synthase, and glyceraldehyde-3-phosphate dehydrogenase. Here, we report two new records of endophytic fungi isolated from L. obtusiloba in Korea: Colletotrichum citricola and Valsa ceratophora. These results enhance our understanding of the diversity of endophytic fungi in L. obtusiloba and contribute to enhancing our knowledge of fungal biodiversity in Korea.

Discovery of the vagrant darter, Sympetrum vulgatum (Odonata: Libellulidae) in Korea

  • Jeong Sun Park;Jong Moon Kim;Hyeong Gi Jeon;Sung Soo Kim;Jee-Young Pyo;Iksoo Kim
    • International Journal of Industrial Entomology and Biomaterials
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    • v.48 no.3
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    • pp.114-123
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    • 2024
  • Among the 17 species of Sympetrum found on the Korean peninsula Sympetrum vulgatum Linnaeus, 1758 had not been recorded since its first record in 1932 by Doi, suggesting the potential for misidentification. We observed a total of four specimens on September 21, 25, 30, and November 1 2022 in Goseong, Gangwon-do and collected each one male and female. We described the morphological and ecological characteristics of this species, conducted molecular analysis using mitochondrial COI, and addressed the controversial past record regarding its presence on the Korean Peninsula.

Homeobox Gene (OSH1) Expression in Embryonic Mutants of Rice (Oryza sativa L.)

  • Hong, Soon-Kwan;Lee, Sang-Lyung;Shin, Young-Boum;Yoon, Kyung-Min;Kim, Nam-Soo
    • Animal cells and systems
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    • v.2 no.1
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    • pp.81-86
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    • 1998
  • Recent identification and characterization of plant homeobox genes suggest that they play important roles in morphogenetic events. OSH1, one of the rice homeobox genes, is thought to be related to organ development since the changes of OSH1 gene expression cause morphological abnormalities of leaves by the ectopic expression and is expressed during early embryogenesis. In this experiment, the expression pattern of OSH1 was analyzedinmutants by in situ hybridization, and OSH1's potential as a molecular marker was explored. Region-specific expression of OSH1 during early embryogenesis shows that OSH1 could be used as a molecular marker for characterizing embryo mutants. Although several organless and shootless mutants showed normal expression of OSM1, some mutants exhibited abnormal expression patterns. In a minute organless cle1-1 embryo whose epidermis resembled morphologically the epithelium of scutellum, OSH1 expression was limited to a small basal region. This expression pattern suggests the gross deletion of the basal part. In a radicleless mutant, odm115, OSH1 expression was detected in a basal region instead of subcentral region of the ventral side. Together with other characteristics (short embryo and normal adventitious roots), odm115 was estimated to be derived from the deletion of basal region. Among five shootless mutants, three showed normal expression of OSH1. In the shl2 embryo, no expression of OSH1 was observed. In the shl1 embryo, however, OSH1 expression was extended to a dorsal side, indicating that SHL2 might be related to dorsoventral patterning. The above results of in situ hybrydization clearly indicate that OSH1 can be utilized as a marker for characterizing gene functions of embryo mutants.

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Assessment of Micro Organic Pollutants Removal Using Advanced Water Treatment Process and Nanofiltration Process (고도처리공정과 나노여과공정에서의 미량유해물질 제거 평가)

  • Kang, Joon-Seok;Choi, Yang-Hun;Kwon, Soon-Buhm;Yu, Young-Beom
    • Journal of Korean Society of Environmental Engineers
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    • v.36 no.8
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    • pp.579-587
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    • 2014
  • Various kinds of micro organic pollutants have frequently been detected from a water system. Therefore, it is considered to be very important part in the drinking water treatment system. And the research about removal process and processing efficiency have been being conducted briskly. In this study, the removal efficiency was evaluated using advanced water treatment process and nanofiltration process. The removal efficiency of nanofiltration process was very different according to physical and chemical characteristics of materials. The molecular weight of cutoff was the most influential factor in the removal efficiency. And when pKa value was higher than pH of raw water or Log Kow value was below 2, the removal efficiency of material was decreased. In case of oxidation reaction, the bigger the molecular weight of material was and the more hydrophobic a material was, the less oxidation reaction occurred. And the removal efficiency was decreased. Most unoxidized materials were removed by absorption. And the more actively oxidation reaction occurred by $H_2O_2$, the more absorption reaction increased.

A Large Genomic Deletion in Gibberella zeae Causes a Defect in the Production of Two Polyketides but not in Sexual Development or Virulence

  • Lee Sun-Hee;Kim Hee-Kyoung;Hong Sae-Yeon;Lee Yin-Won;Yun Sung-Hwan
    • The Plant Pathology Journal
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    • v.22 no.3
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    • pp.215-221
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    • 2006
  • Gibberella zeae (anamorph: Fusarium graminearum) is an important pathogen of cereal crops. This fungus produces a broad range of secondary metabolites, including polyketides such as aurofusarin (a red pigment) and zearalenone (an estrogenic mycotoxin), which are important mycological characteristics of this species. A screen of G. zeae insertional mutants, generated using a restriction enzyme-mediated integration (REMI) procedure, led to the isolation of a mutant (Z43R606) that produced neither aurofusarin nor zearalenone yet showed normal female fertility and virulence on host plants. Outcrossing analysis confirmed that both the albino and zearalenone-deficient mutations are linked to the insertional vector in Z43R606. Molecular characterization of Z43R606 revealed a deletion of at least 220 kb of the genome at the vector insertion site, including the gene clusters required for the biosynthesis of aurofusarin and zearalenone, respectively. A re-creation of the insertional event of Z43R606 in the wild-type strain demonstrated that the 220-kb deletion is responsible for the phenotypic changes in Z43R606 and that a large region of genomic DNA can be efficiently deleted in G. zeae by double homologous recombination. The results showed that 52 putative genes located in the deleted genomic region are not essential for phenotypes other than the production of both aurofusarin and zearalenone. This is the first report of the molecular characterization of a large genomic deletion in G. zeae mediated by the REMI procedure.

Electronic Properties of MIM Structure Organic Thin-films that Manufacture by LB method (LB법으로 제작한 MIM 구조 유기 박막의 전자특성)

  • Choi, Young-Il;Lee, Kyung-Sup;Lim, Jung-Yeol;Song, Jin-Won
    • 전자공학회논문지 IE
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    • v.43 no.4
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    • pp.99-104
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    • 2006
  • The Langmuir-Blodgett(LB) technique has attracted considerable interest in the fabrication of electrical and electronic devices. Maxwell displacement current (MDC) measurement has been employed to study the dielectric property of Langmuir-films. MDC flowing across monolayers is analyzed using a rod-like molecular model. A linear relationship between the monolayer compression speed u and the molecular area Am. Compression speed a was about 30, 40, 50mm/min. Langmuir-Blodgett(LB)layers of Arachidic acid deposited by LB method were deposited onto slide glass as Y-type film. The structure of manufactured device is Au/Arachidic acid/Al, the number of accumulated layers are 9$\sim$21. Also, we then examined of the Metal-Insulator-Metal(MIM) device by means of I-V. The I-V characteristics of the device are measured from -3 to +3[V]. The insulation property of a thin film is better as the distance between electrodes is larger.

A Study on the Enhancement of the Performance of Gas Separation Membranes by Ozone Treatment (오존처리를 이용한 기체분리막의 성능 향상에 관한 연구)

  • 류동현;최승학;오세중;구자경
    • Membrane Journal
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    • v.10 no.4
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    • pp.192-197
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    • 2000
  • Gas permeation and separation characteristics of ozone treated polysulfone (PSF) membranes were investigated. The PSF is one of the most widely used commercial membranes. Ozone treatment of the PSF membrane was performed for certain hours by continuously supplying 5 vol.% ozone-containing oxygen to the chamber, in which the membrane samples were charged. The permselectivity of PSF increased with ozone treatment time, and it was found that one and half hour was enough to ozone treatment. Increase in the selectivity of the membrane for He/$N_2$, HY$N_2$, O:J$N_2$, COJ$CH_4$ by ozone treatment was found to be due to decrease in the permeability of gases with larger molecular size such as $N_2$ and $CH_4$ The decrease in the permeability of gases with smaller molecular size was negligible. The increase in the selectivities of the PSF by ozone treatment would be due to decrease in the free volume of the polymer because of the oxygen complexes generated by the reaction between ozone and the polymer.

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Sensory Characteristics of Dehydrated Ginger Rhizomes Prepared using Recycled Dehydrating Liquid as an Alternative Dehydrating Agent (재사용 탈수액을 탈수제로 이용한 생강의 탈수 및 품질특성)

  • Lee, Hyun-Seok;Kwon, Ki-Hyun;Kim, Byeong-Sam;Cha, Hwan-Soo
    • Food Science and Preservation
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    • v.17 no.3
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    • pp.358-364
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    • 2010
  • Ginger was dried using maltodextrin or recycled dehydration liquid as dehydrating agents, and the quality of dried ginger was compared with that of freeze-dried and hot-air-dried samples in terms of color, moisture content, water activity, dehydration rate, rehydration rate, and sensory properties. Ginger prepared using molecular press dehydration (MPD) retained its original color. The dehydration rate increased when ginger was dried using a dehydrating agent. The rehydration rate was increased in ginger dried using such an agent. Both dehydration and rehydration rates were elevated with increasing concentrations of soluble solids in the dehydration liquid. The sensory qualities of ginger prepared using the MPD method were better than those of freeze-dried and hot air-dried samples. These results indicate that drying of ginger rhizomes using recycled dehydrating liquid is very efficient.

First Report of Waitea Ring Patch caused by Waitea circinata on Zoysiagrass (Zoysiagrass에 Waitea circinata에 의한 Waitea Ring Patch 발생)

  • Kim, Kyung-Duck;Hong, Sung-Chul;Jang, Kong-Man;Han, Muho;Pyee, Jae-Ho;Park, Dae-Sup
    • Weed & Turfgrass Science
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    • v.3 no.4
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    • pp.378-381
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    • 2014
  • A new pathogen was isolated from zoysiagrass-planted park of Jeju island in 2014. Symptoms appeared a type of irregular patches occurring brownish leaf blight, followed by stem and crown rot. The symptom was very similar to that of large patch caused by Rhizoctonia solani, a well-known devastating zoysiagrass disease. The isolate showed thin orange-colored mycelia and screlotia were formed on the medium based on cultural characteristics. The causal agent of the disease was finally identified as Waitea circinata by analysis of ribosomal DNA. On the inoculation test, Waitea circinatae showed strong pathogenicity to the zoysiagrass. The mycelia were obviously observed in the inoculated tissues. This is the first report of Waitea ring patch caused by Waitea circinata on zoysiagrass.