The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Two New Records of Endophytic Fungi Isolated from Lindera obtusiloba in Korea: Colletotrichum citricola and Valsa ceratophora

  • Jae-Eui Cha (Department of Biology Education, Korea National University of Education) ;
  • Yun-Jeong Kim (Department of Biology Education, Korea National University of Education) ;
  • Ji-Won Kim (Department of Biology Education, Korea National University of Education) ;
  • Ahn-Heum Eom (Department of Biology Education, Korea National University of Education)
  • Received : 2024.05.21
  • Accepted : 2024.06.14
  • Published : 2024.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Lindera obtusiloba Blume, a shrub prevalent in the understory vegetation of the deciduous forests in East Asia and Korea, is known for its pharmacological properties, including antioxidant, anti-allergic, and anti-inflammatory activities. Endophytic fungi were isolated from surface-sterilized twigs and leaves of L. obtusiloba collected from Samcheok-si and Jecheon-si in Korea. The fungal strains were identified based on their morphological characteristics and molecular analyses using DNA markers such as internal transcribed spacer, large subunit rDNA, β-tubulin, chitin synthase, and glyceraldehyde-3-phosphate dehydrogenase. Here, we report two new records of endophytic fungi isolated from L. obtusiloba in Korea: Colletotrichum citricola and Valsa ceratophora. These results enhance our understanding of the diversity of endophytic fungi in L. obtusiloba and contribute to enhancing our knowledge of fungal biodiversity in Korea.

Keywords

Acknowledgement

This work was supported by a grant from the National Institute of Biological Resources (NIBR) funded by the Ministry of Environment (MOE) of the Republic of Korea.

References

  1. Kim HB, Lee HY, Lee MS, Lee Y, Choi Y, Kim SY, Choi J. Analysis of the genetic diversity and population structure of Lindera obtusiloba (Lauraceae), a dioecious tree in Korea. J Plant Biotechnol 2023;50:207-14. 
  2. Haque ME, Azam S, Balakrishnan R, Akther M, Kim IS. Therapeutic potential of Lindera obtusiloba: Focus on antioxidative and pharmacological properties. Plants 2020;9:1765. 
  3. Arnold AE. Understanding the diversity of foliar endophytic fungi: Progress, challenges, and frontiers. Fungal Biol Rev 2007;21:51-66. 
  4. Tiwari P, Bae H. Endophytic fungi: Key insights, emerging prospects, and challenges in natural product drug discovery. Microorganisms 2022;10:360. 
  5. Choi HG, Lee HD, Kim SH, Na MK, Kim JA, Lee SH. Phenolic glycosides from Lindera obtusiloba and their anti-allergic inflammatory activities. Nat Prod Commun 2013;8:1934578x1300800212. 
  6. Cui J, Guo T, Chao J, Wang M, Wang J. Potential of the endophytic fungus Phialocephala fortinii Rac56 found in Rhodiola plants to produce salidroside and p-tyrosol. Molecules 2016;21:502. 
  7. Kim CK, Eo JK, Eom AH. Diversity of foliar endophytic fungi isolated from Lindera obtusiloba in Korea. Kor J Mycol 2012;40:136-40. 
  8. Choi JW, Lee JM, Park SY, Eom AH. Acrodontium burrowsianum and Pestalotiopsis humicola: Two previously unrecorded fungal species isolated from conifer leaves in Korea. Kor J Mycol 2022;50:312. 
  9. Gardes M, Bruns TD. ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts. Mol Ecol 1993;2:113-8. 
  10. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: A guide to methods and applications 1990;18:315-22. 
  11. Glass NL, Donaldson GC. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol 1995;61:1323-30. 
  12. Carbone I, Kohn LM. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 1999;91:553-6. 
  13. Templeton MD, Rikkerink EH, Solon SL, Crowhurst RN. Cloning and molecular characterization of the glyceraldehyde-3-phosphate dehydrogenase-encoding gene and cDNA from the plant pathogenic fungus Glomerella cingulata. Gene 1992;122:225-30. 
  14. Tamura K, Stecher G, Kumar S. MEGA11: Molecular evolutionary genetics analysis version 11. Mol Biol Evol 2021;38:3022-7. 
  15. Huang F, Chen G, Hou X, Fu Y, Cai L, Hyde K, Li H. Colletotrichum species associated with cultivated citrus in China. Fungal Divers 2013;61:61-74. 
  16. Ma X, Nontachaiyapoom S, Jayawardena RS, Gentekaki E, Zhou S, Qian Y, Wen T, Kang J. Endophytic Colletotrichum species from Dendrobium spp. in China and northern Thailand. MycoKeys 2018:23. 
  17. Huang S, Guo T, Tang L, Chen X, Mo J, Huang H, Li Q, Shi J, Wei S, Huang S. First report of leaf spot caused by Colletotrichum citricola on cavendish bananas in China. Plant Dis 2022;106:2762. 
  18. Tulasne L, Tulasne C. Selecta Fungorum Carpologia 2: 1-319. Paris: Engl transl of WB Growe-Oxford; 1863. 
  19. Saccardo P. Fungi Gallici lecti a Cl. viris P. Brunaud, CC Gillet, Abb. Letendre, A. Malbranche, J. Therry & Dom. Libert. Series IV. Michelia 1882;2:583-648. 
  20. Old K, Yuan Z, Kobayashi T. A Valsa teleomorph for Cytospora eucalypticola. Mycol Res 1991;95:1253-6. 
  21. Zhu H, Tian C, Fan X. Multigene phylogeny and morphology reveal Cytospora spiraeae sp. nov. (Diaporthales, Ascomycota) in China. Phytotaxa 2018;338:49-62. 
  22. Zhang L, Alvarez LV, Bonthond G, Tian C, Fan X. Cytospora elaeagnicola sp. nov. associated with narrow-leaved oleaster canker disease in China. Mycobiology 2019;47:319-28. 
  23. Li J, Zheng H, Yu Z-F, Qiao M. Colletotrichum litangense sp. nov., isolated as an endophyte of Hippuris vulgaris, an aquatic plant in Sichuan, China. Curr Microbiol 2022;79:161. 
  24. Norphanphoun C, Hyde KD. First report of Colletotrichum fructicola, C. rhizophorae sp. nov. and C. thailandica sp. nov. on Mangrove in Thailand. Pathogens 2023;12:1436. 
  25. Vieira WA, Michereff SJ, de Morais MA, Hyde KD, Camara MP. Endophytic species of Colletotrichum associated with mango in northeastern Brazil. Fungal Divers 2014;67:181-202. 
  26. Suzaki K. Population structure of Valsa ceratosperma, causal fungus of Valsa canker, in apple and pear orchards. J Gen Plant Pathol 2008;74:128-32. 
  27. Wang X, Wei J, Huang L, Kang Z. Re-evaluation of pathogens causing Valsa canker on apple in China. Mycologia 2011;103:317-24. 
  28. Abaya A, Xue A, Hsiang T. Selection and screening of fungal endophytes against wheat pathogens. Biol Control 2021;154:104511. 
  29. Monkai J, Tibpromma S, Manowong A, Mapook A, Norphanphoun C, Hyde KD, Promputtha I. Discovery of three novel Cytospora species in Thailand and their antagonistic potential. Diversity 2021;13:488.