• Title/Summary/Keyword: Molecular Characteristics

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Identification of Multiple Active Forms in Cellulase-xylanase of Aspergillus sp. 8-17 by Active Staining

  • Shin, Pyung-Gyun;Ahn, Jun-Bae;Kim, Chang-Young;Jeong, Won-Hwa;Ryu, Jin-Chang
    • Journal of Microbiology and Biotechnology
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    • v.8 no.1
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    • pp.49-52
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    • 1998
  • A fungal strain able to produce filter paper activity (FPase) was isolated from soil by testing the ability to hydrolyze using filter paper. The isolated strain was identified as an Aspergilus sp. judging from its morphological and microscopical characteristics. The cellulase-xylanase system of Aspergillus sp. 8-17 was detected in situ after gel electrophoresis in the presence of SDS and showed that each protein pattern had a distinct polypeptide composition. ${\beta}$-1,4-Glucanase, cellobiohydrolase, and xylanase activity profiles differ from protein patterns. The Aspergillus sp. 8-17 hydrolytic enzymes responsible for the hydrolysis of ${\beta}$-glucan, MUC, and xylan have multiple active forms.

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Expression of Carboxypeptidase Z cDNA from Absidia zychae in saccharomyces cerevisiae and its characteristics (Saccharomyces cerevisiae에서 Absidia zychae 의 Carboxypeptidase A cDNA 의 발현과 특성)

  • 이병로;김종화
    • Microbiology and Biotechnology Letters
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    • v.23 no.2
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    • pp.150-155
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    • 1995
  • Carboxypeptidase Z(CPZ) cDNA of Absidia zychae was experssed in Saccharomyces cerevisiae. The expressed CPZ(YCPZ) was secreted about 30 mg/l into the medium and has a little higher molecular weight than the wild type CPZ in SDS-PAGE. By the result of N-terminal amino acid sequencing, YCPZ has additional 15 amino acids residues in N-terminus of CPZ. But YCPZ shows no difference with CPZ in enzyme activity and substrate specificity. For the identification of processing mechanism of YCPZ, 36-Arg was changed to 36-Thr by site specific mutagenesis. Mutant YCPZ does not processed at 36-Thr. It was, therefore, concluded that the YCPZ was processed by KEX2. According to endo F treatment, high amount of carbohydrate was N-glycosylated in YCPZ.

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Purification and characteristics of the Polyvinyl Alcohol Oxidase from Pseucomonas cepacia G5Y (폴리비닐 말콜 분해균 Pseudomonas cepaia G5Y의 Polyvinyl alcohol oxidase 정제 및 특성)

  • 장대균;조윤래
    • Microbiology and Biotechnology Letters
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    • v.23 no.2
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    • pp.203-208
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    • 1995
  • The Polyvinyl alcohol (PVA) oxidase is a key enzyme involved in degradation of PVA with PVA hydrolase. The PVA oxidase has been purified to homogeneity from the culture broth of PVA grown Pseudomonas cepacia G5Y strain by ammonium sulfate precipitation, DEAE-cellulose column chromatography, and Sephadex G-150 gel filtration. The molecular weight of the purified enzyme was estimated about 60, 000 daltons by SDS-polyacrylamid gel electrophoresis. The enzyme is most active at 45$\circ$C and at pH 8.5, and is stable below 55$\circ$C and between pH 6 and 9. The enzyme activity was strongly inhibited by Ag$^{2+}$ and Hg$^{2+}$.

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Organic light emitting diodes using Iron(II) and Metal-free Phthalocyanine (Iron(II)과 Metal-free Phthalocyanine의 결정구조 변화에 따르는 유기 발광소자의 발광 특성)

  • 임은주;한우미;이정윤;김명식;이기진
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2001.07a
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    • pp.947-950
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    • 2001
  • We report the characteristics of organic light emitting diodes (OLEDS) by controlling the carrier mobility according to the crystalline of Iron(II) Phthalocyanine(Fe-Pc) and metal-free Phthalocyanine (H$_2$-Pc). In order to change the recombination zone, we controlled the hole mobility by changing the crystal structures of Fe-Pc and H$_2$-Pc. OLEDs were constructed with ITO/Fe-Pc/triphenyl-diamine (TPD)/tris-(8-hydroxyquinoline)aluminum (Alq$_3$)/Al and ITO/H$_2$-Pc/triphenyl-diamine (TPD)/tris-(8-hydroxyquinoline)aluminum (Alq$_3$)/Al. The electroluminescent properties were changed according to the heat-treatments of Fe-Pc and H$_2$-Pc. We observed that the recombination zone and the carrier mobility were changed as the higher occupied molecular orbital levels of Fe-Pc and H$_2$-Pc decreased.

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The Volume Resistivity Characteristics of Linear Low Density Polyethylene/Ethylene Vinyl Acetate Blend Film (선형 저밀도 폴리에틸렌/에틸렌 비닐아세테이트 블렌드 박막의 체적고유저항 특성)

  • 이태훈;신종열;오세영;이충호;이용우;홍진웅
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 1998.11a
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    • pp.195-198
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    • 1998
  • In this paper, the physical and the volume resistivity properties due to linear low density polyethylene(LLDPE)/ethylene vinyl acetate(EVA) blends are studied. In order to measure the volume resistivity properties. the micro electrometer is used, the range of temperature and applying voltage are 25 to 120[$^{\circ}C$], form 100 to 1000[V] respectively. From FT-IR spectrum, LLDPE blended with EVA shows an absence of carbonyl and ether groups. From the experimental result of the volume resistivity properties, it is confirmed that the volume resistivity is decreased, which was attributed to the increase of molecular motions with the increase of temperature.

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Optical and Electrical Characteristics of viologen-diTCNQ thin film prepared by Langmuir-Blodgett Technique (LB법을 이용한 viologen-diTCNQ 박막의 광학적 및 전기적 특성)

  • 이용수;신동명;김태완;강도열
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 1997.04a
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    • pp.45-48
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    • 1997
  • Enhancing the electrical conductivity of the ultrathin organic films is one of the important factors for the development of molecular electronic devices. The Langmuir-Blodgett(LB) technique has recently been attracted interest as the a method of deposition ultrathin films. We have fabricated N-docosyl-N'-methyl viologen-diTCNQ(DMVT) anion radical LB film and investigated the optical and electrical conductivity. We have measured UV/visible and FT-lR spectrum. In ESR spectrum, we confirmed that a half-amplitude linewidth is clearly dependent on both temperature and incident angle, which indicates conducting species change. The in-plane electrical conductivity of 21 layers is approximately 1.37$\times$10$^{-6}$ (S/cm).

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Study for the Real Optical Path in the TFT-LCD 3-dimensional Simulation (TFT-LCD 3차원 시뮬레이션에서의 광 경로에 대한 고려)

  • Choi Kyoung-Uk;Kim Gee-Bum;Park Woo-Sang
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.19 no.2
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    • pp.195-199
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    • 2006
  • We report a novel simulation method to calculate optical transmission considering the real paths of optic introduced in a unit pixel of TFT-LCDs using three-dimensional molecular director simulation of the tensor model. The simulation of optical path transmission profile was carried out by calculating new permittivity considered the real paths of optic in liquid crystal cell. As a result, it was clarified that the electro-optic characteristics such as movement of disclination line, contrast ratio and transmittance profile show a large difference according to the viewing angle compared with the conventional method.

Degradation Characteristics of RTV Silicone Rubber by environmental Factors (환경 인자에 의한 RTV 실리콘 고무의 열화 특성)

  • Cho, Hyun-Wook;Huh, Chang-Suh
    • Proceedings of the KIEE Conference
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    • 1998.11c
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    • pp.866-868
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    • 1998
  • Room temperature vulcanizing(RTV)silicone rubber has been widely used to coat porcelain insulators to render water repellency to prevent formation of water filming on the surface and thus to suppress the leakage current and consequent flashover. However, the electrical property and its hydrophobicity of RTV silicone rubber coating under outdoor conditions may be influenced by many environmental factors. In this study various treatments, such as salt-fog, salt water immerging and UV irradiation were applied to samples for investigation the change of the electrical property and hydrophobicity. As a result the leakage current increased and contact angle decreased as the degradation time longer. But the degraded RTV silicone rubber has recovered its hydrophobicity during the drying time in ambient condition because LMW(Low Molecular Weight)silicone fluid diffused from the bulk to the surface.

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Optical Cmharacterization of Dihydrotetraphenylsilole and Nanoaggregates (Dihydrotetraphenylsilole 및 나노응집체의 광학적 특성)

  • Lee, Sung Gi;Yang, Jinseok;Choi, Tae-Eun;Han, Joungmin;Sohn, Honglae
    • Journal of Integrative Natural Science
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    • v.2 no.1
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    • pp.32-36
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    • 2009
  • The purpose of this project is the synthesis of dihydrosilole and its optical characterization for their applications. Dihydrosilole was synthesized from the reduction reaction of either dichlorosilole or chlorohydrosilole with lithium aluminium hydride. The reaction yield for the dihydrosilole through the latter method was higher. The optical characteristics and AIEE effect of dihydrosilole nanoaggregates was investigated for the purpose of increasing the photoluminescence efficiency. Photoluminescence efficiency of dihydrosilole nanoaggregates increased about 100 times compared to that of molecular state.

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A New Record of Volutella ciliata Isolated from Crop Field Soil in Korea

  • Babu, Anam Giridhar;Kim, Sang Woo;Yadav, Dil Raj;Adhikari, Mahesh;Kim, Changmu;Lee, Hyang Burm;Lee, Youn Su
    • Mycobiology
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    • v.43 no.1
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    • pp.71-74
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    • 2015
  • During a survey of fungal species in South Korea, a species of Volutella ciliata was isolated and described based on the analysis of the internal transcribed spacer region of its rDNA and its morphological characteristics. This is the first record of Volutella ciliata isolated from crop field soil in Korea.