• Title/Summary/Keyword: Molecular Characteristics

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First Report on Fusarium Wilt of Zucchini Caused by Fusarium oxysporum, in Korea

  • Choi, In-Young;Kim, Ju-Hee;Lee, Wang-Hyu;Park, Ji-Hyun;Shin, Hyeon-Dong
    • Mycobiology
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    • v.43 no.2
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    • pp.174-178
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    • 2015
  • Fusarium wilt of zucchini in Jeonju, Korea, was first noticed in May 2013. Symptoms included wilting of the foliage, drying and withering of older leaves, and stunting of plants. Infected plants eventually died during growth. Based on morphological characteristics and phylogenetic analyses of the molecular markers (internal transcribed spacer rDNA and translation elongation factor $1{\alpha}$), the fungus was identified as Fusarium oxysporum. Pathogenicity of a representative isolate was demonstrated via artificial inoculation, and it satisfied Koch's postulates. To our knowledge, this is the first report of F. oxysporum causing wilt of zucchini in Korea.

A New Record of Gongronella butleri Isolated in Korea

  • Babu, A. Giridhar;Kim, Sang Woo;Adhikari, Mahesh;Yadav, Dil Raj;Um, Yong Hyun;Kim, Changmu;Lee, Hyang Burm;Lee, Youn Su
    • Mycobiology
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    • v.43 no.2
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    • pp.166-169
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    • 2015
  • We report the isolation of a Gongronella butleri species and describe it based on the analysis of the internal transcribed spacer region of rDNA and morphological characteristics. G. butleri has been reported as a high chitosan producer in the literature. This is the first record of G. butleri isolated from crop field soil in Korea.

Distribution of Rhizosphere and Endosphere Fungi on the First-Class Endangered Plant Cypripedium japonicum

  • Gang, Geun-Hye;Cho, Gyeongjun;Kwak, Youn-Sig;Park, Eun-Hee
    • Mycobiology
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    • v.45 no.2
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    • pp.97-100
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    • 2017
  • Endangered native plant habitats and populations are rapidly disappearing because of climate and environmental changes. As a representative, the abundance of the first-class endangered wild plant, Cypripedium japonicum, has been rapidly decreasing in Korea. The purpose of this study was to evaluate the distribution of rhizosphere and endophytic fungi on C. japonicum in its native habitat. A total of 440 rhizosphere and 79 endosphere fungi isolates were isolated and identified on the basis of their molecular characteristics. Sixty-five genera and 119 fungi species were identified in this study. The genus Trichoderma showed the highest abundance among both rhizosphere and endosphere fungi. Mortierella, Hypocrea, and Penicillium spp. were also relatively dominant species on C. japonicum. The community structures of rhizosphere and endosphere fungi were similar, but endosphere fungi showed greater diversity.

Intragenic Suppressors for Expory-defective Signal Sequence Mutation of Ribose-binding Protein in Escherichia coli (대장균 리보스 결합단백질의 신호배열 변이에 대한 숙성체 부위의 회복돌연변이)

  • 이영희;송택선;김정호;박순희;박찬규
    • Korean Journal of Microbiology
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    • v.29 no.5
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    • pp.270-277
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    • 1991
  • A mutational alteration in the signal sequence of ribose-binding protein (RBP) of Escherichia coli, rbsB103, completely blocks the export of the protein to the periplasm. Intragenic suppressors for this mutation have been selected on minimal medium with ribose as a sole carbon source. Six suppressor mutations were characterized in detail and were found to have single amino acid wubstitution in the mature portion of RBP, which resulted in the mobility shift of the proteins on SDS polyacrylamide gel. Amino acid changes of these suppressors were localized in several peptides which are packed to form the N terminal domain of typical bilobate conformation of RBP. The involvement of SecB, a molecular chaperone, was investigated in the suppression of signal sequence mutation. Translocation efficency was found to be increased by the presence of SecB for all suppressors. It is likely that the folding characteristics of RBP altered by the suppressor mutations affect the affinity of interaction between SecB and RBP.

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Molecular Biological Characteristics of Ustilago maydis Virus Isolated in Korea

  • Won, Yie-Se;Choi, Hyoung-Tae
    • Korean Journal of Microbiology
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    • v.30 no.3
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    • pp.177-180
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    • 1992
  • Among 120 U. maydis strains isolated in Korea 14 different strains containing specific viral dsRNA segments were analyzed for the distribution of dsRNA and the production of toxin protein. Several distinctive dsRNA patterns were identified, 9 cases of P type with typical H, M and L ds RNA and one case of non-P-type, the frequency of a specific isolate was decreased with increasing number of dsRNA segments. The presence of dsRNA had no effect on the cultural or morphological phenotype of the host. Two isolates containing P type dsRNA segments appeared to produce toxin protein (killer strains) which inhibited the growth of 4 isolates (sensitive strain) with different susceptibility. Two killer strains contain unique M dsRNA segment which may code for toxin protein. However, the presence of toxin-sensitive strains among dsRNA-free isolates was similar to that of ds RNA containing strains.

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Purification and Characterization of the $\beta$-Adrenergic Receptor from Rat Cerebral Cortex

  • 신찬영;김희진;노민수;함경수;강현삼;고광호
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1994.04a
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    • pp.292-292
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    • 1994
  • Catecholamines acting through ${\beta}$-adrenergic receptors regulate a wide range of metabolic activities in mammalian tissue. Of the various receptors coupled to adenylate cyclase, the ${\beta}$-adrenergic receptors are the most extensively characterized and have been purified from both nonmammal ian and mammal inn sources. However, most studies of the molecular properties of ${\beta}$-adrenergic receptors have been confined to peripheral tissues. Less progress has been achieved in characterizing the brain ${\beta}$-adrenergic receptor The goal of the present study was, therefore, to purify and characterize the neurotransmitter receptor proteins. To achieve this goal, the following stepwise experiments were performed. At first, the membrane-bound ${\beta}$-adrenergic receptors were. solubi1ized from brain tissue. Secondly, conditions for affinity chromatography were determined to purify the solubilized receptors effectively. Finally, the large-scale purification was performed and the characteristics of the purified ${\beta}$-adrenergic receptor were examined.

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Focused Electron Beam-Controlled Graphene Field-Effect Transistor

  • Kim, Songkil
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.33 no.5
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    • pp.360-366
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    • 2020
  • Focused electron beams with high energy acceleration are versatile probes. Focused electron beams can be used for high-resolution imaging and multi-mode nanofabrication, in combination with, molecular precursor delivery, in an electron microscopy environment. A high degree of control with atomic-to-microscale resolution, a focused electron beam allows for precise engineering of a graphene-based field-effect transistor (FET). In this study, the effect of electron irradiation on a graphene FET was systematically investigated. A separate evaluation of the electron beam induced transport properties at the graphene channel and the graphene-metal contacts was conducted. This provided on-demand strategies for tuning transfer characteristics of graphene FETs by focused electron beam irradiation.

Measurement of III-V Compound Semiconductor Characteristics using the Contactless Electroreflectance Method

  • Yu, Jae-In;Choi, Soon-Don;Chang, Ho-Gyeong
    • Journal of Electrical Engineering and Technology
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    • v.6 no.4
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    • pp.535-538
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    • 2011
  • The electromodulation methods of photoreflectanceand the related technique of contactless electroreflectance(CER) are valuable tools in the evaluation of important device parameters for structures such as heterojunction bipolar transistors, pseudomorphic high electron mobility transistors, and quantum dots(QDs). CER is a very general principle of experimental physics. Instead of measuring the optical reflectance of the material, the derivative with respect to a modulating electric field is evaluated. This procedure generates sharp, differential-like spectra in the region of interband (intersubband) transitions. We conduct electric-optical studies of both GaAs layers and InAs selfassembled QDs grown by molecular beam epitaxy. Strong GaAsbandgap energy is measured in both structures. In the case of lnAs monolayers in GaAs matrices, the strong GaAsbandgap energy is caused by the lateral quantum confinement.

Atypical Teratoid Rhabdoid Tumour : From Tumours to Therapies

  • Richardson, Elizabeth Anne;Ho, Ben;Huang, Annie
    • Journal of Korean Neurosurgical Society
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    • v.61 no.3
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    • pp.302-311
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    • 2018
  • Atypical teratoid rhabdoid tumours (ATRTs) are the most common malignant central nervous system tumours in children ${\leq}1year$ of age and represent approximately 1-2% of all pediatric brain tumours. ATRT is a primarily monogenic disease characterized by the bi-allelic loss of the SMARCB1 gene, which encodes the hSNF5 subunit of the SWI/SNF chromatin remodeling complex. Though conventional dose chemotherapy is not effective in most ATRT patients, high dose chemotherapy with autologous stem cell transplant, radiotherapy and/or intrathecal chemotherapy all show significant potential to improve patient survival. Recent epigenetic and transcriptional studies highlight three subgroups of ATRT, each with distinct clinical and molecular characteristics with corresponding therapeutic sensitivities, including epigenetic targeting, and inhibition of tyrosine kinases or growth/lineage specific pathways.

Dielectric Properties of Unsaturated Polyester Resin for Electric Safety (전기 안전에 관한 불포화폴리에스테르 수지의 유전특성)

  • 이성일;권호영
    • Journal of the Korean Society of Safety
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    • v.6 no.3
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    • pp.12-20
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    • 1991
  • The dielectric properties that effect to electric safety for unsaturated Pelvester resin were studied over the temperature range of 30-40[$^{\circ}C$] and the frequency range of 110~3$\times$10$^{6}$ [Hz]. According to the results of the study, it has been observed that two kinds of dielectric losses in electric safety were existed by the molecular movement of C-H and OH group. When the ,adio of hardener was 1.5[%], the characteristics of specimens were excellent in view of the electrical materical. In the range of measuring frequency the dielectric constants and the dielectric losses were 3.5~5.5 and 10$^2$~10$^3$ respectly. The activation energy of loss spectrum in aspect of electric safety and the entropy appeared at the 30[$^{\circ}C$] were 33.4[Kacl/mole] and 54.9[cal/mole.deg] respectly.

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