• Title/Summary/Keyword: Molecular Characteristics

Search Result 2,994, Processing Time 0.025 seconds

Modification of the characteristics of silkworm powder by treatment with alkaline protease

  • Kim, Sung-Kuk;Jo, You-Young;Lee, Kwang-Gill;Kim, Hyun-Bok;Kim, Yong Soon;Ju, Wan-Taek;Jung, Da-Eun;Kweon, HaeYong
    • International Journal of Industrial Entomology and Biomaterials
    • /
    • v.31 no.1
    • /
    • pp.30-33
    • /
    • 2015
  • Enzymatic modification of proteins is often used to increase the biological activity of materials. Silkworm powder has been investigated as a functional food resource, but no study has been performed on its modification by commercial food enzyme. Therefore, this study aimed to determine the feasibility of such modification of silkworm powder by alkaline protease. The activity of the enzyme was confirmed using an azocasein assay. Subsequently the silkworm powder was hydrolyzed by enzymatic treatment. UV visible spectrometry showed that the supernatant of silkworm powder subjected to enzymatic treatment had a stronger absorption band than the untreated powder. SDS-PAGE electrophoresis showed that the molecular weight of silkworm powder decreased on enzymatic treatment. Thus the results indicate that commercial enzymes might be used to modify the characteristics of silkworm powder.

Drinking Water Treatment of Surface Water Using Microfiltration-Nanofiltration Processes (정밀여과 및 나노여과 공정을 이용한 지표수의 상수처리)

  • Lee, Sung-Woo;Kim, Chung-Han;Kwak, Dong-Heui
    • Journal of Korean Society of Water and Wastewater
    • /
    • v.14 no.3
    • /
    • pp.224-230
    • /
    • 2000
  • Membrane processes are capable of removing much materials from water. The removal or rejection characteristics of a membrane is usually depend upon the nominal pore size or MWCO(molecular weight cut off). A membrane with a smaller nominal pore size or MWCO should be capable of removing smaller contaminants from water. A series of experiments was performed to investigate the separation characteristics of membrane processes which consisted of microfiltration(MF) and nanofiltration(NF). To evaluate removal efficiencies of some pollutants such as the consumption of $KMnO_4$, THMFP, NH3-N, Fe, Mn, and pesticides, source water sampled from the Kum river was treated by the those membrane processes. Also, the results of experiments were compared with those of conventional water treatment processes. By two types of the membrane process, total removal efficiency of $KMnO_4$ consumed, THMEP, and $NH_3-N$ were 91.0%, 84.3%, and 85.5%, respectively and those processes were efficient in pesticides removal as well. Most of the effluents satisfied the Korean standard of drinking water quality continuously in the experimental periods. However, NF was needed for producing the safe drinking water in case of treating the raw water contaminated with Mn since removal efficiency of MF was not high enough. On the basis of the experimental results, it was suggested that NF could be applied to remove not only $NH_3-N$ but THMFP even without pre-chlorination.

  • PDF

Chemical Characteristics of Abiotic-Stressed Tobacco Stems for the Utilization of a Non-Wooden Biomass (비목질 재료의 바이오매스화를 위한 환경 스트레스 담배줄기의 화학조성)

  • Kim, Kang-Jae;Hong, Sung-Bum;Eom, Tae-Jin
    • Journal of Korea Technical Association of The Pulp and Paper Industry
    • /
    • v.48 no.1
    • /
    • pp.53-60
    • /
    • 2016
  • Abiotic-stressed tobacco stems as a non-wooden biomass were analyzed for their chemical characteristics. Light-stressed tobacco stems (LST) have a relatively high nitrogen concentration, much more extractive content, and a similar amount of lignin and higher contents of acid sugars than those of Non stressed tobacco (NST). It also has low cellulose crystallinity and a high degree of condensation. Guaiacyl units having a lower molecular weight distribution consist of rich lignin. Tension stressed tobacco (TST) growth differentiation under tensile stress was significantly different between normal tissue and cell walls, with the exception of the slightly higher cellulose crystallinity observed for.

Tribological Characteristics of Si-Diamond-Like Carbon Films in a Condition with Carbon Nanotube Ink Lubricant (Carbon Nanotube 잉크 환경에서의 Si-Diamond-Like Carbon 박막의 내마모 특성)

  • Jang, Kil-Chan;Kim, Tae-Gyu
    • Korean Journal of Materials Research
    • /
    • v.21 no.3
    • /
    • pp.149-155
    • /
    • 2011
  • We investigated tribological characteristics of diamond-like carbon (DLC) in a condition with carbon nanotube (CNT) content of 1wt% in aqueous solution. Si-DLC films were deposited by radio frequency plasma enhanced chemical vapor deposition (RF-PECVD) process on Al6061 aluminum alloy. In this study, the deposition of DLC films was carried out in vacuum with a chamber pressure of 10-5 to 10-3 Torr achieved by mechanical pump followed by turbo molecular pump. The surface adsorbed oxygen on the Aluminum substrates was removed by passing Ar gas for 10 minutes. The RF power was maintained at 500W throughout the experiment. A buffer layer of HMDSO was deposited on the substrate to improve the adhesion of DLC coating. At this point CH4 gas was introduced in the chamber using gas flow controller and DLC coating was deposited on the buffer layer along with HMDSO for 50 min. The thickness of 1 ${\mu}m$ was obtained for DLC films on aluminum substrates The tribological properties of as synthesized DLC films were analyzed by wear test in the presence of dry air, water and lubricant such as CNT ink.

Identification of Orchid Mycorrhizal Fungi Isolated from Epipactis thunbergii in Korea (닭의난초(Epipactis thunbergii)에 공생하는 난 균근균의 분리 및 동정)

  • Han, Han-Kyeol;Chung, Jae-Min;Cho, Yong-Chan;Kim, Dae-Shin;Eom, Ahn-Heum
    • The Korean Journal of Mycology
    • /
    • v.41 no.1
    • /
    • pp.9-13
    • /
    • 2013
  • In this study, roots of Epipactis thunbergii were collected from Chujado on the north of Jeju-do. Six fungal isolates were isolated from surface-sterilized roots of the orchid and classified with groups based on morphological characteristics. Fungal DNA was extracted from each isolate and amplified ITS region using ITS1-OF/ITS4-OF primer pair. Three species of orchid mycorrhizal fungi were identified as Tulasnella calospora, Tulasnella sp. and Sebacina sp. based on molecular and morphological characteristics.

Unrecorded Fungal Species Isolated from Medicinal Plant Leaves in Korea (약용식물 잎에서 분리한 국내 미기록 진균)

  • Ahn, Geum Ran;Noh, Hyeungjin;Lee, Dong Hyeung;Kim, Susan;Kim, Jun Young;Kim, Seong Hwan
    • The Korean Journal of Mycology
    • /
    • v.47 no.4
    • /
    • pp.319-328
    • /
    • 2019
  • As an effort to explore fungal diversity, fungal survey was undertaken in 2018 in Jangheung, Korea. For the survey, medicinal plant leaves were sampled from Selaginella tamariscina, Serratula coronata ssp. insularis, Scutellaria baicalensis, Scrophularia buergeriana and subjected to fungal isolation. Four unrecorded fungal species, Paraboeremia selaginellae, Colletotrichum camelliae, Alternaria eichhorniae, and Phomopsis capsici were obtained from the survey. This study described their morphological characteristics including colony features formed on media, light microscopic images and molecular characteristics of nucleotide sequences of the ITS and 28S rDNA regions.

Two Unrecorded Enophytic Fungi Isolated from Root of Ligusticum chuanxiong in Korea: Pithomyces chartarum and Plectosphaerella niemeijerarum (토천궁의 뿌리에서 분리된 2종의 국내 미기록 내생균: Pithomyces chartarum and Plectosphaerella niemeijerarum)

  • Park, Hyeok;Jung, Chung Ryul;Eom, Ahn-Heum
    • The Korean Journal of Mycology
    • /
    • v.47 no.4
    • /
    • pp.329-335
    • /
    • 2019
  • Endophytic fungal strains were isolated from the sterilized roots of a medicinal plant, Ligusticum chuanxiong and identified based on morphological characteristics and molecular analysis of internal transcribed spacer, large subunit rDNA, and beta-tubulin DNA regions. Our results confirmed the presence of Pithomyces chartarum and Plectosphaerella niemeijerarum in the fungal strains. To the best of our knowledge, the fungal strains have not been reported in Korea. In this report, we describe morphological characteristics and phylogenetic trees of these endophytic fungal strains.

Two Unrecorded Endophytic Fungi Isolated from Funaria hygrometrica in Korea (표주박이끼(Funaria hygrometrica)에서 분리된 2종의 국내 미기록 내생균)

  • Choi, Hyun-Sook;Park, Hyeok;Eom, Ahn-Heum
    • The Korean Journal of Mycology
    • /
    • v.47 no.4
    • /
    • pp.313-318
    • /
    • 2019
  • In the present study, we isolated endophytic fungal strains from the rhizoids of the moss Funaria hygrometrica. The isolated strains were identified based on morphological characteristics and analysis of the internal transcribed spacer (ITS) and large subunit (LSU) rDNA sequence regions. Consequently, we confirmed the presence of two endophytic fungal species, Curvularia protuberata and Didymella anserina, which have not been reported in Korea previously. Here, we describe the morphological characteristics and molecular analysis results of these fungal species.

Compression and Adhesion Characteristics of Rice Dough Added with Cellulose Ethers Using Rheometer (유변물성 측정기를 이용한 셀룰로오스 에테르가 첨가된 쌀 반죽의 압축 및 접착 특성)

  • Um, In Chul;Yoo, Young Jin
    • Current Research on Agriculture and Life Sciences
    • /
    • v.32 no.1
    • /
    • pp.18-23
    • /
    • 2014
  • The present study examined the effect of cellulose ether on the compression and adhesion characteristics of rice dough using a rheometer. When increasing the amount of hydroxypropyl methylcellulose (HPMC), the adhesion strength consistently increased. However, the compression strength of the rice dough was the highest with 2% HPMC. When increasing the molecular weight and decreasing the water content, the compression and adhesion strength of the rice dough were both increased. Furthermore, the substitution type and degree of cellulose ether were also found to be key factors determining the compression and adhesion strength of the rice dough.

Yeast as a Touchstone in Post-genomic Research: Strategies for Integrative Analysis in Functional Genomics

  • Castrillo, Juan I.;Oliver, Stephen G.
    • BMB Reports
    • /
    • v.37 no.1
    • /
    • pp.93-106
    • /
    • 2004
  • The new complexity arising from the genome sequencing projects requires new comprehensive post-genomic strategies: advanced studies in regulatory mechanisms, application of new high-throughput technologies at a genome-wide scale, at the different levels of cellular complexity (genome, transcriptome, proteome and metabolome), efficient analysis of the results, and application of new bioinformatic methods in an integrative or systems biology perspective. This can be accomplished in studies with model organisms under controlled conditions. In this review a perspective of the favourable characteristics of yeast as a touchstone model in post-genomic research is presented. The state-of-the art, latest advances in the field and bottlenecks, new strategies, new regulatory mechanisms, applications (patents) and high-throughput technologies, most of them being developed and validated in yeast, are presented. The optimal characteristics of yeast as a well-defined system for comprehensive studies under controlled conditions makes it a perfect model to be used in integrative, 'systems biology' studies to get new insights into the mechanisms of regulation (regulatory networks) responsible of specific phenotypes under particular environmental conditions, to be applied to more complex organisms (e.g. plants, human).