• Title/Summary/Keyword: Mold Analysis

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Diffusible and Volatile Antifungal Compounds Produced by an Antagonistic Bacillus velezensis G341 against Various Phytopathogenic Fungi

  • Lim, Seong Mi;Yoon, Mi-Young;Choi, Gyung Ja;Choi, Yong Ho;Jang, Kyoung Soo;Shin, Teak Soo;Park, Hae Woong;Yu, Nan Hee;Kim, Young Ho;Kim, Jin-Cheol
    • The Plant Pathology Journal
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    • v.33 no.5
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    • pp.488-498
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    • 2017
  • The aim of this study was to identify volatile and agardiffusible antifungal metabolites produced by Bacillus sp. G341 with strong antifungal activity against various phytopathogenic fungi. Strain G341 isolated from four-year-old roots of Korean ginseng with rot symptoms was identified as Bacillus velezensis based on 16S rDNA and gyrA sequences. Strain G341 inhibited mycelial growth of all phytopathogenic fungi tested. In vivo experiment results revealed that n-butanol extract of fermentation broth effectively controlled the development of rice sheath blight, tomato gray mold, tomato late blight, wheat leaf rust, barley powdery mildew, and red pepper anthracnose. Two antifungal compounds were isolated from strain G341 and identified as bacillomycin L and fengycin A by MS/MS analysis. Moreover, volatile compounds emitted from strain G341 were found to be able to inhibit mycelial growth of various phytopathogenic fungi. Based on volatile compound profiles of strain G341 obtained through headspace collection and analysis on GC-MS, dimethylsulfoxide, 1-butanol, and 3-hydroxy-2-butanone (acetoin) were identified. Taken together, these results suggest that B. valezensis G341 can be used as a biocontrol agent for various plant diseases caused by phytopathogenic fungi.

Convergent Case Study of Research and Education: Internet of Things Based Wireless Device Forming Research (R&E 융합적 사례: IoT 기반 무선 디바이스 성형연구)

  • Lee, Jeong-Ick
    • Journal of the Korea Convergence Society
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    • v.6 no.4
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    • pp.1-7
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    • 2015
  • This study is a case study of the research study and education with high school students of natural sciences. Recent development of the Internet of Things (IoT) based on the subject of various studies exist. This study is one of the most portable communication devices of these infancy, yet students were easy to define the molding process of contacting a pager that can go along way in making research topics. We called a pager. Today, it is given a large and complex smart mobile communication devices that can be used for big data. Prior pagers are taught the meaning of the first mobile communication means in our lives were given device. The internal structure is relatively simple and is thought to function relatively simple, just suitable as a teaching practices of high school students with an interest in science and engineering universities. This study can see all of the mechanical, electronic information contents of the radio pager in a relatively simple analysis of the injection molding ONE CAVITY analysis. Furthermore, the students also rated good educational practices that give meaning to remind the convergence training on mass production and process automation.

Analysis on the Impact of Composite by Using FEM (유한요소법을 이용한 복합재료의 충격에 관한 해석)

  • Kim, Sung-Soo;Kim, Young-Chun;Hong, Soon-Jik;Kook, Jeong-Han;Kim, Sei-Hwan;Cho, Jae-Ung
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.2
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    • pp.542-547
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    • 2013
  • In this study, mechanical property on the composite material of aluminum foam core is investigated by simulation analysis. Impact energies such as 50J, 70J, and 100J are applied to the specimen model. The maximum load occurs at 3.4ms for 50J, 3.2ms for 70J, and 3.2ms for 100J respectively. The striker penetrates the upper face sheet, causing the core to be damaged at 50J test but the lower face sheet remains intact with no damage. It results in occurring with the energy of 52 J. At 70J test, it penetrates the upper face sheet and penetrated the core. And the striker causes the lower face sheet with damage. And it results in occurring with the energy of 65 J. Finally at 100J test, the striker penetrated both the upper face sheet and core and even the lower face sheet. The load becomes maximum at the time when striker penetrates through the upper plate and it rapidly reduced. And then the load increases rapidly when reaching the lower face sheet. And it decreases again. It results in occurring with the energy of 95 J.

The Study of Compaction Characteristics of Cataclasite Fill Material using Large and Standard Compaction Tests (대형다짐시험 및 표준다짐시험을 이용한 파쇄암 성토재의 다짐특성 연구)

  • Jeoung, Jae-Hyeung;Ryu, Sang-Hun;Choi, Dong-Yub;Park, Kwang-Sik;Hwang, Sung-Pil
    • The Journal of Engineering Geology
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    • v.26 no.4
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    • pp.439-445
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    • 2016
  • The new airports apply the systematic runway foundation construction to build the high quality runways in order to take the leading position to win future aerial demands and stay ahead of competitors. This study is intended to supplement the weakness of existing standard compaction test to minimize residual settlement of lower weak foundation during operation of passenger berthage. The fill material was sampled from 4 construction sites using the fill material with diameter of 100mm or less, and the standard compacting test (KS F 2312), large circular mold compacting test, and water-replacement field density test (ASTM D 5030) were conducted. The regression analysis of correlation of the field density test and the standard indoor compaction test showed the unreliable value at P-value of 0.05, and the regression analysis of the field density test and the large indoor compaction test showed the high correlation with R value of 0.8878. It is judged that the construction of overall uniform quality can be assured as the site condition is truly reflected only if the compacting test method is selected in consideration of maximum size of fill material when evaluating the maximum dry density used in design and construction.

Intraspecies Volatile Interactions Affect Growth Rates and Exometabolomes in Aspergillus oryzae KCCM 60345

  • Singh, Digar;Lee, Choong Hwan
    • Journal of Microbiology and Biotechnology
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    • v.28 no.2
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    • pp.199-209
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    • 2018
  • Volatile organic compounds (VOCs) are increasingly been recognized as the chemical mediators of mold interactions, shaping their community dynamics, growth, and metabolism. Herein, we selectively examined the time-correlated (0 D-11 D, where D = incubation days) effects of intraspecies VOC-mediated interactions (VMI) on Aspergillus oryzae KCCM 60345 (S1), following co-cultivation with partner strain A. oryzae KACC 44967 (S2), in a specially designed twin plate assembly. The comparative evaluation of $S1_{VMI}$ (S1 subjected to VMI with S2) and its control ($S1_{Con}$) showed a notable disparity in their radial growth ($S1_{VMI}$ < $S1_{Con}$) at 5 D, protease activity ($S1_{VMI}$ > $S1_{Con}$) at 3-5 D, amylase activity ($S1_{VMI}$ < $S1_{Con}$) at 3-5 D, and antioxidant levels ($S1_{VMI}$ > $S1_{Con}$) at 3 D. Furthermore, we observed a distinct clustering pattern for gas chromatography-time of flight-mass spectrometry datasets from 5 D extracts of $S1_{VMI}$ and $S1_{Con}$ in principle component analysis (PC1: 30.85%; PC2: 10.31%) and partial least squares discriminant analysis (PLS-DA) (PLS1: 30.77; PLS2: 10.15%). Overall, 43 significantly discriminant metabolites were determined for engendering the metabolic variance based on the PLS-DA model (VIP > 0.7, p < 0.05). In general, a marked disparity in the relative abundance of amino acids ($S1_{VMI}$ > $S1_{Con}$) at 5 D, organic acids ($S1_{VMI}$ > $S1_{Con}$) at 5 D, and kojic acid ($S1_{VMI}$ < $S1_{Con}$) at 5-7 D were observed. Examining the headspace VOCs shared between S1 and S2 in the twin plate for 5 D incubated samples, we observed the relatively higher abundance of C-8 VOCs (1-octen-3-ol, (5Z)-octa-1,5-dien-3-ol, 3-octanone, 1-octen-3-ol acetate) having known semiochemical functions. The present study potentially illuminates the effects of VMI on commercially important A. oryzae's growth and biochemical phenotypes with subtle details of altered metabolomes.

A STUDY ON THE TENSILE STRENGTH OF REINFORCED VENEERING COMPOSITE RESINS FOR CROWN (강화형 치관용 복합레진의 인장강도에 관한 연구)

  • Ahn, Seung-Geun;Kang, Dong-Wan
    • The Journal of Korean Academy of Prosthodontics
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    • v.38 no.2
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    • pp.226-241
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    • 2000
  • Recently a new generation of crown and bridge veneering resins containing submicron glass fillers was introduced. These ultrasmall particle hybrid composite materials distinguish themselves, compared with conventional microfill crown and bridge resins, through improved mechanical properties. It is claimed that these composites are suitable for metal free crowns and even bridges using fiber reinforcement. The purpose of this study was to evaluate the effect of thermal cycling on the tensile strength of the following veneering composites: Artglass(Heraeus Kulzer Co., Wehrheim, Germany), Estonia(Kuraray Co.. Japan), Sculpture(Jeneric Pentron Co., Wallingford, U.S.A.), and Targis(Ivoclar Co., Schaan Liechenstein). According to manufacturer's instructions, rectangular tensile test specimens measuring $1.5{\times}2.0{\times}4.5mm$ were made using a teflon mold. Whole specimens were divided into two groups. One group was dried in a desiccator at $25^{\circ}C$ for 10 days, and another group was subjected to thermal cycling($10,000{\times}$) in water($5/55^{\circ}C$). All test specimens were placed in a universal testing machine and loaded until fracture with a crosshead speed of 0.5mm/min. Weibull analysis and Tukey's test were used to analyze the data. The fracture surfaces of specimens were observed in SEM and the aliphatic C=C absorbance peak of Estenia and Targis resin was analyzed using Fourier transform infrared(FTIR) spectroscopy. Within the limitations imposed in this study, the following conclusions can be drawn: 1. Both in drying condition and thermal cycling condition, the highest tensile strength was observed in Estenia testing group(p<0.05). 2. The strength data were at to single-mode Weibull distribution, and the Weibull modulus of all veneering composite resin specimens increased after thermal cycling treatment. 3. After thermal cycling test, the highest tensile strength was observed in the Estenia group, and the lowest value was observed in the Targis group. The tensile strength values showed the significant differences between each group(p<0.05) 4. The aliphatic C=C absorbance peak of Estonia and Targis resin was decreased after light curing, and there was no distinct change after thermal cycling.

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Analysis of RTM Process Using the Extended Finite Element Method (확장 유한 요소 법을 적용한 RTM 공정 해석)

  • Jung, Yeonhee;Kim, Seung Jo;Han, Woo-Suck
    • Composites Research
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    • v.26 no.6
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    • pp.363-372
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    • 2013
  • Numerical simulation for Resin Transfer Molding manufacturing process is attempted by using the eXtended Finite Element Method (XFEM) combined with the level set method. XFEM allows to obtaining a good numerical precision of the pressure near the resin flow front, where its gradient is discontinuous. The enriched shape functions of XFEM are derived by using the level set values so as to correctly describe the interpolation with the resin flow front. In addition, the level set method is used to transport the resin flow front at each time step during the mold filling. The level set values are calculated by an implicit characteristic Galerkin FEM. The multi-frontal solver of IPSAP is adopted to solve the system. This work is validated by comparing the obtained results with analytic solutions. Moreover, a localization method of XFEM and level set method is proposed to increase the computing efficiency. The computation domain is reduced to the small region near the resin flow front. Therefore, the total computing time is strongly reduced by it. The efficiency test is made with a simple channel flow model. Several application examples are analyzed to demonstrate ability of this method.

Selecting Plants for the Extensive Rooftop Greening Based on Herbal Plants (초화류를 중심으로한 관리조방적 옥상녹화용 식물 소재 선정)

  • Lee, Eun-Heui;Cho, Eun-Jin;Park, Min-Young;Kim, Dong-Wook;Jang, Seong-Wan
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.10 no.2
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    • pp.84-96
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    • 2007
  • The purpose of this study was to select herbaceous plants that can be used for presenting various views and biodiversity on the extensive rooftop greening. Experiment plots were constructed in July 2005 on the rooftop of the Administration Building in the Seoul Women's University. For this experiment, planters were used to design rooftop greening. The size of a planter is 500mm${\times}$500mm${\times}$100mm and each planter has the water storage plate in the lower part of it. The soil was constructed by mixing pearlite, vermiculite, cocopeat, and leaf mold in the ratio of 6 : 2 : 1 : 1. The plot was divided into the watered plot and the dry plot. Since each plot was constructed 2 times, finally 4 planters were constructed in total. One hundred species were used for the experiment and 9 plants per species were planted in each planter. Plants were organized according to types of plants and the experiment used 86 native herbaceous plants, 6 herbs, and 8 foreign plants. The plots were monitored once a month, from July to November 2005. The length and width of plants were tape-measured and covering rate was calculated by CAD program. "SPSS 10.1" was used for a statistical analysis. The result showed no significant difference between the watered plots and the dry plots. In cases of some plants, there were statistically significant differences between the watered planter and dry planter such as follows : Astilbe chinensis and Polygonatum odoratum which are shade plants were measured as the highest value on the watered pots, and Aquilegia buergeriana, Chrysanthemum zawadskii, Calendula arvensis and Gypsophila cerastioides D.Don which are sunny plants were measured as the highest value on the dry plots. According to the final analysis of the data collected and observed for growth condition during the first year of the research, 51 species including Prunella vulgaris var. lilacina and Veronica linaiaefolia in native herbaceous plant, 5 species with Lavandula angustifolia in herbs, and 3 species with Lantana camara and Muscari armeniacum in foreign plants showed the highest growth condition. In conclusion, it is suggested that various plants including sedums could be effectively used for extensive rooftop greening to improve landscape(a view) of the rooftop and increase ecological values.

A Convergent Investigation on the Air flow in Driving According to a Cargo Container and the Wind Deflector (트럭 화물칸 및 윈드 디플렉터에 따른 운행중 공기흐름에 대한 융합 연구)

  • Choi, Kye-Kwang;Cho, Jae-Ung
    • Journal of the Korea Convergence Society
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    • v.11 no.9
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    • pp.175-180
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    • 2020
  • In this study, the freight vehicles were modelled and the flow analysis on the existence or non-existence of a cargo container and the wind deflector were carried out. Based on the driving speed of 100 km/hr, at all models A, B and C, the highest flow rate was shown between 58 m/s and 59 m/s at the top of the model shape. All models A, B and C showed the highest pressure of air resistance between 652Pa and 671Pa at the front of the model geometry. The maximum pressure of model A is considered to be the smallest, with the least flow resistance to speed compared to models B and C. Therefore, it can be seen that model A has an advantageous condition for air resistance in terms of fuel costs. Unlike model B which causes the rapid flow resistance at the cargo compartment, model C can be found to flow a little more smoothly on the streamlined wind deflector. So, the flow air at a streamlined shape is considered to be more advantageous in terms of air resistance than at angular shape. By applying the research analysis result on the air flow in driving according to a cargo container and the wind deflector, it is seen that this study is adequate at the practical efficient design and aesthetic convergence.

A Quality Stability Estimation of Shock-absorber Tube for automatic drawer (자동서랍함용 완충기 튜브의 품질 안정성 예측)

  • Son, Jae-Hwan;Kim, Young-Suk;Han, Chang-Woo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.12 no.7
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    • pp.2919-2924
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    • 2011
  • The automatic drawer is used to absorb the movement shock and adjust its velocity when it opens and closes. The tube in shock-absorber is the cylindrical case which surrounds its parts and is made of acetal. The purpose of this study is to determine the quality stability of the tube in the shock-absorber in injection molding process. The tube which had been manufactured in the process with 4 cavity cooling unit was used. In this study, the analysis and test are carried out to determine its quality stability. Which are the quality analysis with numerical simulation and performance tests of the tube compared with one of foreign make. It is calculated that the injection press is 87.6 MPa and the deflections in X, Y, Z directions are ranged in 0.07~1.00 mm. When the researched tube is compared with the foreign made tube, the maximum bending compressive load is 231 kgf higher, average axial compressive load is 0.05 kgf higher, and the roughness(Ra) on the inner surface is $0.02\;{\mu}m$. lower. In the result, it is known that the quality of researched tube in injection mold process is stable and its performance is superior.