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Comparison of Semen Characteristics, Frozen-Thawed Sperm Viability, Testosterone Concentration and Embryo Development between Yorkshire Boar A and B

  • Yi, Y.J.;Lee, S.H.;Park, C.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.5
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    • pp.612-616
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    • 2004
  • This study was carried out to compare the semen characteristics, frozen-thawed sperm viability and testosterone concentration and in vitro fertilization (IVF) and development of in vitro matured pig oocytes between two Yorkshire boars. Semen and blood samples were collected once per week from October to November 2002 from two adult Yorkshire boars at 18 months of age with 170 kg body weight. Sperm were deep frozen in 5 ml maxi-straws with lactose-egg yolk and N-acetyl-D-glucosamine (LEN) diluent and stored in liquid nitrogen. Blood samples were obtained at 10 a.m. by inserting a 21 gauge, hypodermic needle attached to 10 ml syringe into surface veins in the ear. The concentration of testosterone was determined by Competitive Enzyme Immunoassay. Ovaries were collected from prepubertal gilts at a local slaughter house. Cumulus oocyte complexes were aspirated from antral follicles (3 to 6 mm in diameter). The medium used for oocyte maturation was modified TCM 199. After about 22 h of culture, oocytes were cultured without cysteamine and hormones for 22 h at $38.5^{\circ}C$, 5% $CO_2$ in air. For IVF, one frozen 5 ml straw was thawed at $52^{\circ}C$in 40 sec and was diluted with 20 ml Beltsville thawing solution at room temperature. Sperm were washed 2 times in mTLP-PVA and inseminated without preincubation after thawing. Oocytes were inseminated with $2{\times}10^7$/ml sperm concentration. Oocytes were coincubated for 6 h in 500 ${\mu}$l mTBM fertilization medium. At 6 h after IVF, oocytes were transferred into 500 ${\mu}$l NCSU-23 culture medium for further culture of 48 and 144 h. There were no significant differences in the semen volume, motility, normal acrosome morphology and sperm concentration of raw semen between A and B of Yorkshire boar. However, motility and normal acrosome of boar A were higher than those of boar B at 0.5, 2, 3, 4, 5 and 6 h incubations of frozen-thawed sperm. Testosterone concentration (3.75 ng/ml) of boar A was higher than that (2.34 ng/ml) of boar B. The rate of blastocyst formation (15.1%) of boar A was higher than that (10.4%) of boar B. In conclusion, serum testosterone concentration of boar showed very important role for the frozen-thawed sperm viability and the blastocyst formation of pig oocytes matured in vitro.

Face Detection in Color Images Based on Skin Region Segmentation and Neural Network (피부 영역 분할과 신경 회로망에 기반한 칼라 영상에서 얼굴 검출)

  • Lee, Young-Sook;Kim, Young-Bong
    • The Journal of the Korea Contents Association
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    • v.6 no.12
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    • pp.1-11
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    • 2006
  • Many research demonstrations and commercial applications have been tried to develop face detection and recognition systems. Human face detection plays an important role in applications such as access control and video surveillance, human computer interface, identity authentication, etc. There are some special problems such as a face connected with background, faces connected via the skin color, and a face divided into several small parts after skin region segmentation in generally. It can be allowed many face detection techniques to solve the first and second problems. However, it is not easy to detect a face divided into several parts of regions for reason of different illumination conditions in the third problem. Therefore, we propose an efficient modified skin segmentation algorithm to solve this problem because the typical region segmentation algorithm can not be used to. Our algorithm detects skin regions over the entire image, and then generates face candidate regions using our skin segmentation algorithm For each face candidate, we implement the procedure of region merging for divided regions in order to make a region using adjacency between homogeneous regions. We utilize various different searching window sizes to detect different size faces and a face detection classifier based on a back-propagation algorithm in order to verify whether the searching window contains a face or not.

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A Study of the Baekdudaegan and Ridgelines Extraction and Environmental Impact Assessment Utilizing GIS (GIS를 활용한 백두대간·정맥 추출 및 환경성평가 방안 연구)

  • Lee, Moung-Jin;Lee, Soo-Jae
    • Journal of the Korean Association of Geographic Information Studies
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    • v.16 no.3
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    • pp.136-146
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    • 2013
  • This study has specified terminology for mountain ridgeline, and organized the present condition of Korean mountain ridgeline and empirical management measures of Beakdudeagan and Jeongmeak. Moreover, based on previously published paper maps, mountain map, and numerical maps, this study has used GIS to reorganize Jeongmaek of Korea, including Hanbukjeongmaek, Hannamjeongmaek, and Gumbukjoengmaek. To ensure accuracy, DEM, Shaded Relief Image, gradient, curvature, and aspect have been analyzed from recent numerical maps. On extraction process, this study analyzes more specifically the results, previous Beakdudaegan is modified and new Jeongmaeks have been extracted. Moreover, for analyzing natural environment of surrounding of major mountain ridgeline, ecological zoning grade of mountain ridgeline is analyzed. With the analyzed result, domestic and foreign mountain ridgeline management policy is suggested, and environmental impact assessment method for major mountain ridgeline development is also suggested. In this study, Baekdudaegan and its Jeongmaek are organized and analyzed. With the results, this study suggested political linkage of mountain ridgelines and guidelines for environmental impact assessment of ridgeline development.

Surface Modification of Poly(vinylidene fluoride) Membranes using Surface Modifying Macromolecules (SMMs) and Their Application to Pervaporation Separation (SMMs을 이용한 고분자막의 표면개질과 이의 투과증발분리 연구)

  • Rhim, Ji-Won;Lee, Byung-Seong;Kim, Dae-Hoon;Lee, Bo-Sung;Yoon, Seok-Won;Im, Hyeon-Soo;Moon, Go-Young;Nam, Sang-Yong;Byun, Hong-Sik
    • Membrane Journal
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    • v.18 no.3
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    • pp.206-213
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    • 2008
  • Poly(vinylidene fluoride) (PVDF) membrane surfaces were modified using surface modifying macromolecules (SMMs). The Zonyl BA-L as SMM was used and the various PVDF membranes containing 0 to 2 wt% SMM were prepared. The resulting membranes were characterized through SEM, contact angle measurements and pervaporation separation of water-ethanol system. SMM layers were created in the surface regions of PVDF membranes by SEM images and the contact angles were increased more than untreated PVDF membranes. The pervaporation was carried out at 50, 60 and $70^{\circ}C$, and the PVDF membranes containing 1 and 2 wt% SMM were used for 10, 20, 50 wt% water in the binary water/ethanol mixtures and pure water. PVDF/2 wt% Zrlnyl BA-L membrane showed the permeability 5.3 $g/m^2hr$ and separation factor 287 at $50^{\circ}C$ for water : ethanol = 10 : 50 solution.

Overview of Technology for Fixation of Carbon Dioxide Using Microalgae (미세조류를 이용한 이산화탄소 고정화 기술 현황)

  • Jeon, Seon-Mi;Kim, In Hae;Ha, Jong-Myung;Lee, Jae-Hwa
    • Applied Chemistry for Engineering
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    • v.19 no.2
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    • pp.145-150
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    • 2008
  • In this work we have studied the antifouling properties of the hydrophobic sol-gel modified sensing membrane and its optical properties for sensor application. E. coli JM109, B. cereus 318 and P. pastoris X-33 were cultivated in confocal cultivation dishes with glass surface, respectively. The glass surface was coated with the hydrophobic sol-gels prepared by the dimethoxy-dimethyl-silane (DiMe-DMOS) and tetramethyl-orthosilicate (TMOS). After cultivation, microorganisms adhered on the surface coated with sol-gels and glass surface were dyed by gram-staining method and the numbers of microorganisms were analyzed based on the image data of the scanning electronic microscope (SEM). A great number of microorganisms, about $2{\sim}3{\times}10^4/mm^2$, was adhered on the glass surfaces which no hydrophobic sol-gels were coated. But, the antifouling effect of the hydrophobic sol-gels was large, that microorganisms of less than $200{\sim}300/mm^2$ were adhered on the coated glass surface. The performance of the sensing membranes for detection of pH and dissolved oxygen was enhanced by recoating the light insulation layer prepared with the mixture of the hydrophobic sol-gel and graphite particles.

Management Strategies and the Growth Stages Analysis of Local Festival : Cases of Hampyeong Butterfly Festival and Hwacheon Sancheoneo Ice Festival (지역축제의 성장단계별분석과 관리전략 : 함평나비축제와 화천산천어축제를 중심으로)

  • Kim, Hyeonwook
    • The Journal of the Korea Contents Association
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    • v.15 no.5
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    • pp.537-549
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    • 2015
  • The purpose of this study is to analyze the characteristics of two local festivals, already recognized as a successful regional cultural festival, over a period of time to apply the product life cycle theory. As a result of the analysis of the two festivals, in introduction stage, the festival organizers have focused mainly on settling down the festival's core programs and raising awareness on the subject of the festival for the stimulation for basic demands. Second, for maintaining increasing demands, the qualitative improvement of the core program, the development of new programs and the expansion of the programs for the visitors' convenience and safety were focused on. In addition, strategies for promoting awareness of the festival had modified the strategy to promote on the contents and programs of the festival, as well as public relations strategy, not only domestic but also the foreign countries, was established and fulfilled. Lastly, in maturity stage, to overcome declining the number of visitors and economic effect both festivals have showed providing sophisticated programs for the visitors' convenience and safety, improving service quality through the development of the existing programs, providing economic benefits such as admission cuts or giving gift certificates and expanding number of foreign visitors with strengthening the promotion that was implemented in growth stage and enhancing the better image of the festival through the social contribution. Therefore, strategies for the each stage mentioned above present the significant policy implications for festival organizers who were planning to establish a new festival or implementing a festival with experiencing the tepid growth.

Velocity Model Building using Waveform Inversion from Single Channel Engineering Seismic Survey (탄성파 파형역산을 이용한 엔지니어링 목적의 단일채널 탄성파 탐사자료에서의 속도모델 도출)

  • Choi, Yeon Jin;Shin, Sung Ryul;Ha, Ji Ho;Chung, Woo Keen;Kim, Won Sik
    • Geophysics and Geophysical Exploration
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    • v.17 no.4
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    • pp.231-241
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    • 2014
  • Recently, single channel seismic survey for engineering purpose have been used widely taking advantage of simple processing. However it is very difficult to obtain high fidelity subsurface image by single channel seismic due to insufficient fold coverage. Recently, seismic waveform inversion in multi channel seismic survey is utilized for accurate subsurface imaging even in complex terrains. In this paper, we propose the seismic waveform inversion algorithm for velocity model building using a single channel seismic data. We utilize the Gauss-Newton method and assume that subsurface model is 1-Dimensional. Seismic source estimation technique is used and offset effect is also corrected by removing delay time by offset. Proposed algorithm is verified by applying modified Marmousi2 model, and applied to field data set obtained in port of Busan.

The Experimental Study on the Absorbed Energy of Carbon/Epoxy Composite Laminated Panel Subjected to High-velocity Impact (고속 충격을 받는 Carbon/Epoxy 복합재 적층판의 흡수 에너지 예측에 대한 실험적 고찰)

  • Cho, Hyun-Jun;Kim, In-Gul;Lee, Seokje;Woo, Kyeongsik;Kim, Jong-Heon
    • Composites Research
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    • v.26 no.3
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    • pp.175-181
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    • 2013
  • The evaluation and prediction for the absorbed energy, residual velocity, and impact damage are the key things to characterize the impact behavior of composite laminated panel subjected to high-velocity impact. In this paper, the method to predict the residual velocity and the absorbed energy of Carbon/Epoxy laminated panel subjected to high velocity impact are proposed and examined by using quasi-static perforation test and high-velocity impact test. Total absorbed energy of specimen due to the high-velocity impact can be grouped with static energy and kinetic energy. The static energy are consisted of energy due to the failure of the fiber and matrix and static elastic energy, which are related to the quasi-static perforation energy. The kinetic energy are consisted of kinetic energy of moving part of specimen, which are modelled by three modified kinetic model. The high-velocity impact test were conducted by using air gun impact facility and compared with the predicted values. The damage area of specimen were examined by C-scan image. In the high initial impact velocity above the ballistic limit, both the static energy and the kinetic energy are known to be the major contribution of the total absorbed energy.

In vitro study of compressive fracture strength of Empress 2 crowns cemented with various luting agents

  • Kim Min-Ho;Yang Jae-Ho;Lee Sun-Hyung;Chung Hun-Young;Chang Ik-Tae
    • The Journal of Korean Academy of Prosthodontics
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    • v.39 no.3
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    • pp.260-272
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    • 2001
  • All-ceramic restorations have had a more limited life expectancy than metal ceramic restorations because of their low strength. Their relatively lower strength and resistance to fracture have restricted the use of all-ceramic crowns to anterior applications where occlusal loads are lower. But there has been increasing interest in all-ceramic restorations because patients are primarily concerned with improved esthetics. Many efforts have been made to in prove the mechanical properties of dental ceramics. This study was designed to elucidate the influence of the luting agent on the strength of the Empress 2 crown (staining technique) cemented on human teeth. Seventy extracted human permanent molar teeth were chosen. Teeth were prepared for Empress 2 crowns with milling machine on a surveyor. A dental bur was placed in the mandrel that was positioned so that the long axis of the bur was perpendicular to the surveyor base. Dimensions of the Empress 2 crown preparation were $6^{\circ}$ taper on each side, $1.5{\pm}0.1mm$ shoulder margin, and 4mm crown height. The luting cements used in this study were as follow: 1. Uncemented 2. Zinc phosphate cements (Confi-Dental) 3. Conventional glass ionomer cement : Fuji 1 (GC) 4. Resin-modified glass ionomer cements : Fuji plus (GC) 5. Adhesive cements : Panavia F (Kuralay), Variolink II (Vivadent), Choice (Bisco). Fracture test using Instron. The crowns were loaded in compressive force to evaluate the effect of these cements on the breaking strength of these all-ceramic crowns. A steel ball with a diameter of 4mm was placed on the occlusal surface and load was applied to the steel ball by a cylindrical bolt with a crosshead speed of 0.5mm per minute until fracture occurred. The fractured surface was examined using Scanning Electron Microscopic Image (SEM) to discover the correlation between fracture strength and bonding capacity. Within the limitation of this in vitro study design, the results were as follows : 1. fomentations significantly increased the fracture resistance of Empress ceramic crowns compared to control. Uncemented (206.9 N): ZPC (812.9 N): Fuji 1 (879.5 N): Fuji Plus (937.7 N): Choice (1105.4 N): Variolink II (1221.1 N): Panavia F (1445.2 N). 2. Resin luting agent, treated by a silane bond enhancing agents, yielded a significant increase in fracture resistance. In some of the Panavia F group, a fracture extended into dentin. 3. According to SEM images of fractured Empress crowns, the stronger the bond at both interfaces(crown and die), the more fracture strength was acquired.

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A Study on the 'fragmentation' trend of modern film montage (현대영화 몽타주의 '파편화(fragmentation)' 경향 연구)

  • LEE, Jiyoung
    • Trans-
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    • v.3
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    • pp.29-53
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    • 2017
  • The film scholar Vincent Amiel divides into three types of montage through his book The Aesthetics of Montage ; Montage narratif, Montage discursif, and Montage decorrespondances. These three categories are the concept that encompasses the aesthetic class to which most movies belong. Early films pursued the essential and basic functions of editing, which tend to be modified in the direction of enhancing the director's goals over time. In this way, "Expressive Montage" is one of most important concepts of montage, not as a 'methodology' that combines narrative but as a 'purpose'. In the montage stage, the expressive montage work is done through three steps of decision. The process of 'combining' to combine the selected films in a certain order, after the process of 'selection' which selects only necessary parts of the rush film, and 'connection' to determine the scene connection considering the duration of the shot. The connection is the final stage of the montage. There are exceptions, of course. When fiction films of classical narratives use close-ups, or when using models or objects of neutered animals, the film induces the tendency of a "montage decorrespondances" rather than a "montage narratif" or "montage discursif". This study attempts to analyze the tendency of montage of works with 'uncertain connection' through 'collage' used by close-ups and montage decorrespondances as 'fragmentation tendency of modern films'. The fragmentation of the montage in contemporary film breaks the continuous and structural nature of the film, and confuses the narration structure that is visible on the surface of the film. The tendency of the fragmentation of the montage, which started from this close-up, seems to give an answer to the extensibility of the modern image.

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