• Biomedical Science Letters
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• v.25 no.4
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• pp.372-380
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• 2019

Mice with specific modified genes are useful means of studying development and disease. The CRISPR/Cas9 system is a very powerful and effective tool for generating genetically modified mice in a simple and fast manner. To generate human IgG light kappa constant knock-in mice, we tested by microinjection of a mixture of Cas9 protein, single-guide RNA and target homologous recombinant donor DNA into zygotes. We found that the injection of 10 ng/μL of Cas9 protein and crRNA/tracrRNA, rather than single guide RNA, induced the production of knock-in mice more effectively. Thus, our study provides valuable information that will help to improve the production of knock-in mice and contribute the successful generation of humanized Ab-producing mice in Korea.

• The Magazine of the Society of Air-Conditioning and Refrigerating Engineers of Korea
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• v.17 no.5
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• pp.575-582
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• 1988

This paper presents an energy analysis on the VAV system of 10-story building in Seoul using DOE-2 program and Modified Bin Method. The use of VAV system is an excellent means of saving energy in new buildings and/or retrofit. The fan control techniques considered are cycling, discharge dampers, inlet vanes, and motor speed control. The monthly and annual energy consumption is calculated for fan control techniques, components, and chiller types. The results of annual fan electricity use are compared using DOE-2 and Modified Bin Method, and show fairly good agreement. In addition, factors affecting energy consumption are also described.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 2003.09a
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• pp.431-434
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• 2003

In this paper we introduce a modified objective function for fuzzy c-means clustering with logistic regression model in the presence of noise cluster. The logistic regression model is commonly used to describe the effect of one or several explanatory variables on a binary response variable. In real application there is very often no sharp boundary between clusters so that fuzzy clustering is often better suited for the data.

• Journal of the Korean Society of Safety
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• v.17 no.3
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• pp.50-55
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• 2002

In this numerical study, characteristics of swirl generation by the fan and selection of the location of the fan was studied theoretically by the modified TEACH code. The governing equations for the system are solved by means of the k dimensional version of the SIMPLE method and STAGGERED grid. From the present results, the optimal position of the fm is 0.625(h/H).

• Journal of mucopolysaccharidosis and rare diseases
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• v.2 no.1
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• pp.17-18
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• 2016

Enzyme replacement therapy (ERT) is a well-established means of treating lysosomal storage disease (LSD). However, classical IV infusion based ERT method produces less than ideal results, especially, CNS defects and quality of life in patients. To improve these main problems of parental IV formulation for LSDs, we investigate modified ERT method and evaluated the efficacy in animal model.

• The Korean Nurse
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• v.30 no.4
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• pp.61-68
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• 1991

This study was conducted to testing the criterion - related validity of a mental health assessment tool which developed based on a korean culture. Criteria scale for this tool were MMPI and CMI(M - R). The study subject were 100 male and female aged 20 or more with quota sampling. The data was collected from August 16. to August 26. 1989. The data obtained from 85 subjects were analysed using S.P.S.S.(Stastistical Package for the Social Science). As a result, there are no significant correlation between Mental Health Assessment Tool and MMPI and CMI except Mf(Masculinity-Feminity) Subscale of MMPI. This result means the MMPI and CMI was not related to tool which developed based on medical model from etic perspectives, although the tool which had been developed in America Modified to Korean situation. So I dare to say that only the absence of mental illness does not means mental health and the diagnosis of mental illness is not the only criteria of a mental health.

• Korean Journal of Food Science and Technology
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• v.31 no.1
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• pp.62-67
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• 1999

Periodate-oxidized soluble starch and maltohexaose, maltotetraose, maltose, and glyceraldehyde reacted with sweet potato ${\beta}-amylase$, wheat ${\beta}-amylase$, aldolase, bovine serum albumin, catalase, carboxypeptidase, ferritin and pronase. Electrophoretical mobility of modified proteins was different from that of native proteins, and modified proteins were stained with periodic acid-Schiff while native proteins did not stain. This results means that oxidized sugars attached on proteins. This bond is based on the Schiffs base between CHO group of oxidized sugar and ${\varepsilon}-NH_2$ group of lysine of protein. There is no changed UV absorption spectrum of sweet potato ${\beta}-amylase$ modified with oxidized soluble starch, in comparison with native enzyme.

• Journal of the Korean Society of Physical Medicine
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• v.12 no.4
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• pp.133-138
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• 2017

PURPOSE: The purpose of this study was to investigate the effect of modified motor developmental intervention on the SCM muscle thickness and the range of motion (ROM) of neck in subjects with congenital muscular torticollis. METHODS: Thirteen subjects who had congenital muscular torticollis were participated in this study and they were offered the modified motor developmental interventions for 8 weeks by pediatric physical therapist. SCM thickness of affected and non-affected side and ROM of neck rotation were collected between before and after intervention. SCM thickness was measured by using Ultrasound Unit (IU 22, Ultrasound system, Philips, Netherlands) and ROM of neck was measured by using the goniometer (Arthrodial protractor, North coast, USA). Data were represented as means ${\pm}SD$. Paired t-test was used to determine the effect of interventions on the SCM muscle thickness and neck ROM. RESULTS: SCM thickness of affected side was significantly decreased after the modified motor developmental interventions and that of non-affected side was generally increased but it was not significantly difference between pre and post interventions. ROM of neck rotation and lateral flexion was significantly increased after interventions. CONCLUSION: These results suggested that the modified motor developmental interventions could improve the recovery of SCM muscle thickness and neck ROM.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.11 no.3
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• pp.1700-1721
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• 2017

The problem of blocking artifacts is very common in block-based image and video compression, especially at very low bit rates. In this paper, we propose a post-processing method for JPEG-coded image deblocking via sparse representation and adaptive residual threshold. This method includes three steps. First, we obtain the dictionary by online dictionary learning and the compressed images. The dictionary is then modified by the histogram of oriented gradient (HOG) feature descriptor and K-means cluster. Second, an adaptive residual threshold for orthogonal matching pursuit (OMP) is proposed and used for sparse coding by combining blind image blocking assessment. At last, to take advantage of human visual system (HVS), the edge regions of the obtained deblocked image can be further modified by the edge regions of the compressed image. The experimental results show that our proposed method can keep the image more texture and edge information while reducing the image blocking artifacts.

• Proceedings of the Korean Powder Metallurgy Institute Conference
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• 2006.09b
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• pp.822-823
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• 2006

The compaction and sintering behavior of zirconium titanate $(ZrTiO_4)$ was investigated by means of the measurement of sintering density and shrinkage, and the observation of microstructure. With increasing the content of $Al_2O_3$ additive, $Al_2O_3$-modified zirconium titanate samples fired at $1300^{\circ}C$ showed the anisotropic shrinkage behavior that the upper region of sintered body has higher sintering shrinkage than the low region. This difference of sintering shrinkage decreased with increasing firing temperature from 1300 to $1400^{\circ}C$. The SEM micrographs of powder compation show that the anisotropic shrinkage behavior is related with non-uniform density in a uniaxial compaction.