• 대한의생명과학회지
• /
• 제21권3호
• /
• pp.135-143
• /
• 2015

Electrocardiogram measures the electric impulses generated by the heart during its cycle. Recently genome-wide association studies on electrocardiogram traits revealed many relevant genetic loci. Therefore, these findings need to be validated and investigated to determine the underlying mechanisms using mouse models. Invasive radiotelemetry has been widely used to record the electrocardiogram in mice because it has several advantages over non-invasive measurements. However, radiotelemetry is expensive and requires complicated surgery. On the other hand, a non-invasive method using 3 electrodes (one for earth) for lead II is easy to establish and allows for rapid measurement. In this study, eleven mice were measured with this non-invasive method and no statistical difference among them was found in any ECG measurements. In addition, repeat measurement in the same mouse was performed in 9 sets of experiment and the results indicated that non-invasive method was reliable for reproducibility. Further it was shown that measurements for 1, 5, 10, and 15 minutes were not different so that a short recording such as 5 minutes was enough to estimate the ECG values including heart rate. Further this method was validated by measuring the ECG of Balb/c and FVB that were previously shown to differ in ECG values by radiotelemetry. Significant differences were found in heart rate, PR interval and corrected QT interval between these mouse strains. This study partially proved that non-invasive method also could provide the accuracy and reproducibility. Based on these results, the non-invasive ECG recordings of lead II is recommended as a useful method for quick test in mouse model.

• 벤처창업연구
• /
• 제10권5호
• /
• pp.93-103
• /
• 2015

주거환경은 거주자의 독특한 특성에 영향을 받는 물리적인 측면뿐만 아니라 사회적, 경제적, 문화적 등 많은 환경적 요소들과 밀접하게 관련되어 있다. 본 연구는 주거선택속성의 이론을 바탕으로 서초구의 이미지와 지각된 가치를 매개로 애호도에 미치는 영향을 규명하고, 경험적으로 주거환경의 선택속성이 애호도와의 관계에서 이미지와 지각된 가치의 매개효과를 분석하고자 한다. 분석방법은 설문지를 배포하여 SPSS 통계 프로그램을 사용하여 분석하였으며, 그 결과는 다음과 같다. 주거선택속성은 지역 명성, 녹색환경, 편리성, 부동산 가치, 안전성, 주택상태의 6개 요인으로 나타났으며, 분석결과 주거선택속성은 애호도에 정적인 영향을 미치는 것으로 나타났다. 둘째, 매개효과를 검증하기 위해 3단계 회귀분석을 사용한 결과 이미지와 지각된 가치는 정적인 영향을 미침으로서 매개효과가 규명되었다. 이러한 결과는 서초구의 애호도를 증가시키기 위해서는 물리적인 환경요소뿐만 아니라 이미지와 지각된 가치를 관리하는 것이 필요하다.

• 한국습지학회지
• /
• 제19권1호
• /
• pp.132-143
• /
• 2017

유사의 침식 이송 퇴적으로 인해 발생할 수 있는 문제를 최소화하기 위해 하천에서의 정기적인 유사량 자료의 획득이 필수이다. 하지만 현재 국내에서의 유사량 측정 계획으로는 유사량 추정에 어려움이 있어 이를 대체하기 위해 경험식이나 수치모형을 활용하고 있다. 따라서 본 논문에서는 유역에서의 유사량 산정과 자료의 연속성 확보를 위해 국내에서 개발된 격자기반 강우-유출-유사 모형인 K-DRUM 모형을 적용하였다. 17개의 중권역에 대한 유량과 유사량을 산정하고 실측자료와 비교를 통해 모형의 적용성을 검토하였다. 정량적인 평가를 위해 NSE, PBIAS, RSR 등을 기준항목으로 사용하였으며, 유량에 대한 모의결과는 강우의 경향을 잘 반영하며 높은 통계값을 나타내었다. 유사량의 경우 유역의 토양침식과정을 물리적으로 잘 반영하였으며, 실측자료를 이용하여 보정을 수행하였을 때 그 유역에서 연속적인 유사량 자료를 산정하는데 적용성이 우수한 것으로 나타났다.

• 한국지리정보학회지
• /
• 제21권1호
• /
• pp.1-11
• /
• 2018

MMS(Mobile Mapping System) 자료를 이용한 정밀 공간 정보 매핑은 고정밀 3차원 지형 모델 구축, 시설물 관리를 위해 중요하며, 특히 도로 중앙선 매핑 작업은 정밀 도로 지도 구축을 위해 필요하다. 본 연구에서는 MMS LiDAR(Light Detection And Ranging) 자료를 이용하여 정밀 공간 정보인 도로 중앙선을 매핑 하는 반자동화 방법을 개발하였다. 우선 주어진 MMS LiDAR 자료를 기반으로 보간법을 이용하여 반사강도 영상을 제작하고, 에지 검출기를 이용하여 반사강도 영상으로부터 선형 세그먼트들을 추출하였다. 최종적으로 추출된 선형 세그먼트들 중에서 도로 중앙선 세그먼트를 수동으로 선택하였다. 추출된 도로 중앙선의 정확도 검증 결과, 0.065m의 정확도를 보여주었으며, 도로 중앙선이 도로 신호와 인접한 일부 지역에서 에러가 발견되었다.

• 전기화학회지
• /
• 제5권2호
• /
• pp.79-85
• /
• 2002

열역학적 관점에서 몬테 카를로 방법의 전기화학적 리튬 인터칼레이션에로의 응용에 대하여 다루었다. 우선 통계 열역학의 앙상블, Ising 및 lattice gas 모델의 기본 개념을 간단히 소개하였고, lattice gas 모델에 근거한 몬테카를로 방법을 사용하여 전이금속 산화물내로의 리튬 인터칼레이션의 열역학을 해석하였다. 특히 $LiMn_2O_4$전극에 대해 전극 포텐셜 곡선과 리튬 이온의 부분 몰 내부에너지와 엔트로피와 같은 열역학적 특성을 다루었고, 이로부터 리튬 인터칼레이션의 전기화학분야에서 몬테 카를로 방법의 유용성을 확인하였다

• Journal of Microbiology and Biotechnology
• /
• 제18권1호
• /
• pp.110-117
• /
• 2008

Three kinds of prenylated flavonols, icariside I, icariside II, and icaritin, were isolated from an icariin hydrolysate and their effects on melanogenesis evaluated based on mushroom tyrosinase inhibition and quantifying the melanin contents in melanocytes. Although none of the compounds had an effect on tyrosinase activity, icariside II and icaritin both effectively inhibited the melanin contents with an $IC_{50}$ of 10.53 and $11.13{\mu}M$, respectively. Whereas icariside II was obtained from a reaction with ${\beta}$-glucosidase and cellulase, the icariin was not completely converted into icariside II. Thus, for the high-purity production of icariside II, the reaction was optimized using the response surface methodology, where an enzyme concentration of 5.0mg/ml, pH 7, $37.5^{\circ}C$, and 8 h reaction time were selected as the central conditions for the central composite design (CCD) for the enzymatic hydrolysis of icariin into icariside II using cellulase. Empirical models were developed to describe the relationships between the operating factors and the response (icariside II yield). A statistical analysis indicated that all four factors had a significant effect (p<0.01) on the icariside II production. The coefficient of determination $(R^2)$ was good for the model (0.9853), and the optimum production conditions for icariside II was an enzyme concentration of 7.5mg/ml, pH 5, $50^{\circ}C$, and 12 h reaction time. A good agreement between the predicted and experimental data under the designed optimal conditions confirmed the usefulness of the model. A laboratory pilot scale was also successful.

• Journal of Animal Science and Technology
• /
• 제60권4호
• /
• pp.7.1-7.5
• /
• 2018

Background: Mice are widely accepted research models of great clinical significance. Maintenance of laboratory mice breed is an essential aspect for performing research activities in various fields of science. Infanticide is one of the prominent causes of litter loss during maintenance of laboratory mice stock. The present study is an effort to monitor the effect of change in ambient temperature of female mice below the normal range on cannibalism and infanticide during early postparturition phase. Adult female Swiss albino mice have been divided into two groups of control and treatment. On the day of litter group one was maintained under controlled temperature conditions (minimum $20^{\circ}C$ to maximum $23^{\circ}C$) throughout, while female mice belong to group two have been exposed to variation of room temperature (maximum $15^{\circ}C$ to minimum $10^{\circ}C$ for two nights and one day) until 36 h postparturition. Results: The effects of temperature changes were observed on the infanticide behaviour of dams along with the survival of pups in early postparturition phase till 36 h after delivery. The significant statistical difference (P < 0.05) was reported in infanticide behaviour of dams when control and treatment group was compared. It is observed that decrement in surrounding temperature promotes decrement in the ambient body temperature of dams during early postparturition. It is proposed that alteration of hypothalamic homeostasis due to temperature change induces cannibalism and infanticide behaviour. Lack of thermoregulation during early postparturition creates the sense of insecurity, in-satiety, anxiety and stress. Conclusions: Authors strongly recommend the maintenance of body and surrounding temperature to prevent infanticidal behaviour and cannibalism within Swiss albino mice population. Further investigations are advisable to authenticate the active behavioural and biochemical pathway behind the phenomena.

• Journal of Korean Society for Atmospheric Environment
• /
• 제18권E4호
• /
• pp.203-213
• /
• 2002

Receptor modeling is one of statistical methods to achieve reasonable air pollution strategies. In order to maintain and manage ambient air quality, it is necessary to identify sources and to apportion its sources for ambient particulate matters. The main purpose of the study was to survey seasonal trends of inorganic elements in the coarse and fine particles. Second, this study has attempted emission sources qualitatively by a receptor method, the PMF mo-del. After that. both PMF (positive matrix factorization) model and TTFA (target transformation factor analysis) model were applied to compare and to estimate mass contribution of coarse and fine particle sources at the receptor. A total of 138 sets of samples was collected from 1989 to 1996 by a low volume cascade impactor with 9 size fraction stages at Kyung Hee University in Korea. Sixteen chemical species (Si, Ca, Fe, K, Pb, Na, Zn, Mg, Ba, Ni, V, Mn, Cr, Br, Cu. Co) were characterized by XRF. The study result showed that the weighted arithmetic mean of coarse and fine particles were 51.3 and 54.4 $\mu\textrm{g}$/㎥, respectively. Contribution of both particle fractions were esti-mated using TTFA and PMF models. The number of estimated sources was seven according to TTFA model and 8 according to PMF model. Comparison of TTFA and PMF revealed that both methodologies exhibited similar trends in their contribution pattern. However, large differences between contributions were observed in some sour-ces. The results of this study may help to suggest control strategies in local countries where known source profiles do not exist.

• 대한의용생체공학회:의공학회지
• /
• 제31권4호
• /
• pp.285-291
• /
• 2010

Recently, advance on various modalities of diagnosing, prostate volume estimation became possible not only by the existing two-dimension medical images data but also by the three-dimensional medical images data. In this study, magnetic resonance image (MRI), computer tomography (CT) and ultrasound (US) were employed to evaluate prostate phantom volume measurements for estimation, comparison and analysis. For the prostate phantoms aimed at estimating the volume, total of 17 models were developed by using devils-tongue jelly and changing each of the 5ml of capacity from 20ml to 100ml. For the volume estimation through 2D US, the calculation of the diameter with C9-5Mhz transducer was conducted by ellipsoid formula. For the volume estimation through 3D US, the Qlab software (Philips Medical) was used to calculate the volume data estimated by 3D9-3Mhz transducer. Moreover, the images by 16 channels CT and 1.5 Tesla MRI were added by the method of continuous cross-section addition and each of imaginary prostate model's volume was yielded. In the statistical analysis for comparing the availability of volume estimation, the correlation coefficient (r) was more than 0.9 for all indicating that there were highly correlated, and there were not statistically significant difference between each of the correlation coefficient (p=0.001). Therefore, the estimation of prostate phantom volume using three-dimensional modalities of diagnosing was quite closed to the actual estimation.

• 응용통계연구
• /
• 제18권1호
• /
• pp.83-98
• /
• 2005

다변량 자료를 분석하는 데 있어서 관측 개체들의 분포적 양태를 파악하는 것은 자료 특성의 이해에 도움이 될 뿐만 아니라 이후 모형화 과정에도 큰 도움을 준다. 이를 위하여 다변량자료의 저차원 시각화에 대한 많은 연구가 진행되어 왔다. 그 중 하나가 코호넨(T. Kohonen)의 자기조직화지도(Self-Organizing Map; SOM)이다. SOM은 저차원 그리드 공간에 고차원 다변량 자료를 축약하여 시각적으로 나타내는 비지도 학습법의 일종으로 최근 들어 통계 분석자들이 많은 관심을 가지고 있는 분야이다. 그러나 SOM은 개체공간의 연속형으로 표현되는 개체를 저차원 그리드 공간에 승자노드에 의해 비연속적으로 표현한다는 단점을 지니고 있다. 본 논문에서는 SOM을 통계적 목적으로 사용하기 위해 요구되는 그리드 공간에 개체를 연속적으로 표현하는 방법들을 제안하고 환용 예를 제시 하고자 한다.