• Toxicological Research
• /
• v.34 no.3
• /
• pp.221-229
• /
• 2018

Tenofovir nanoparticles are novel therapeutic intervention in human immunodeficiency virus (HIV) infection reaching the virus in their sanctuary sites. However, there has been no systemic toxicity testing of this formulation despite global concerns on the safety of nano drugs. Therefore, this study was designed to investigate the toxicity of Tenofovir nanoparticle (NTDF) on the liver and kidney using an animal model. Fifteen adult male Sprague-Dawley (SD) rats maintained at the animal house of the biomedical resources unit of the University of KwaZulu-Natal were weighed and divided into three groups. Control animals (A) were administered with normal saline (NS). The therapeutic doses of Tenofovir (TDF) and nanoparticles of Tenofovir (NTDF) were administered to group B and C and observed for signs of stress for four weeks after which animals were weighed and sacrificed. Liver and kidney were removed and fixed in formal saline, processed and stained using H/E, PAS and MT stains for light microscopy. Serum was obtained for renal function test (RFT) and liver function test (LFT). Cellular measurements and capturing were done using ImageJ and Leica software 2.0. Data were analysed using graph pad 6, p values < 0.05 were significant. We observed no signs of behavioural toxicity and no mortality during this study, however, in the kidneys, we reported mild morphological perturbations widening of Bowman's space, and vacuolations in glomerulus and tubules of TDF and NTDF animals. Also, there was a significant elevation of glycogen deposition in NTDF and TDF animals when compared with control. In the liver, there were mild histological changes with widening of sinusoidal spaces, vacuolations in hepatocytes and elevation of glycogen deposition in TDF and NTDF administered animals. In addition to this, there were no significant differences in stereological measurements and cell count, LFT, RFT, weight changes and organo-somatic index between treatment groups and control. In conclusion, NTDF and TDF in therapeutic doses can lead to mild hepatic and renal histological damage. Further studies are needed to understand the precise genetic mechanism.

• Korean journal of food and cookery science
• /
• v.26 no.6
• /
• pp.707-716
• /
• 2010

Germinated brown rice is a useful material that contains natural nutrients and biologically active substances. This study was conducted to develop a optimal composite recipe for functional germinated brown rice cookie with added spinach (spinacia oleracea L) powder and with high preference in all age groups. The experimental design utilized herein was based on the Central composite design methodology of response surface, which included 16 experimental points, including 2 replicates for spinach, sugar, and butter. The physical, mechanical, and sensory properties of the test were measured, and these values were applied to the mathematical models. A canonical form and perturbation plot showed the influence of each ingredient on the mixed final product. The results of spread ratio decreased significantly with increases in spinach powder, sugar, and butter(p<0.01). L value shows that lightness decreased significantly with an increase in sugar(p<0.05), but the a and b values were not significant. Sensory evaluation found significant values for color(p<0.05), flavor(p<0.05), texture(p<0.05) and overall quality(p<0.05) in the predicted model. The optimum formulation by the numerical and graphical methods was calculated as follows: spinach powder 3 g, sugar 35.5 g butter 54.4 g.

• Magazine of the Korean Society of Agricultural Engineers
• /
• v.40 no.5
• /
• pp.91-99
• /
• 1998

The widespread use of thin shell structures has created a need for a systematic method of analysis which can adequately account for arbitrary geometric form and boundary conditions as well as arbitrary general type of loading. Therefore, the stress and analysis of thin shell has been one of the more challenging areas of structural mechanics. A wide variety of numerical methods have been applied to the governing differential equations for spherical and cylindrical structures with a few results applicable to practice. The analysis of axisymmetric spherical shell is almost an every day occurrence in many industrial applications. A reliable and accurate finite element analysis procedure for such structures was needed. Dynamic loading of structures often causes excursions of stresses well into the inelastic range and the influence of geometry changes on the response is also significant in many cases. Therefore both material and geometric nonlinear effects should be considered. In general, the shell structures designed according to quasi-static analysis may fail under conditions of dynamic loading. For a more realistic prediction on the load carrying capacity of these shell, in addition to the dynamic effect, consideration should also include other factors such as nonlinearities in both material and geometry since these factors, in different manner, may also affect the magnitude of this capacity. The objective of this paper is to demonstrate the dynamic characteristics of spherical shell. For these purposes, the spherical shell subjected to uniformly distributed step load was analyzed for its large displacements elasto-viscoplastic static and dynamic response. Geometrically nonlinear behaviour is taken into account using a Total Lagrangian formulation and the material behaviour is assumed to elasto-viscoplastic model highly corresponding to the real behaviour of the material. The results for the dynamic characteristics of spherical shell in the cases under various conditions of base-radius/central height(a/H) and thickness/shell radius(t/R) were summarized as follows : The dynamic characteristics with a/H. 1) AS the a/H increases, the amplitude of displacement in creased. 2) The values of displacement dynamic magnification factor (DMF) were ranges from 2.9 to 6.3 in the crown of shell and the values of factor in the mid-point of shell were ranged from 1.8 to 2.6. 3) As the a/H increases, the values of DMF in the crown of shell is decreased rapidly but the values of DMF in mid-point shell is increased gradually. 4) The values of DMF of hoop-stresses were range from 3.6 to 6.8 in the crown of shell and the values of factor in the mid-point of shell were ranged from 2.3 to 2.6, and the values of DMF of stress were larger than that of displacement. The dynamic characteristics with t/R. 5) With the thickness of shell decreases, the amplitude of the displacement and the period increased. 6) The values of DMF of the displacement were ranged from 2.8 to 3.6 in the crown of shell and the values of factor in the mid-point of shell were ranged from 2.1 to 2.2.

• Applied Chemistry for Engineering
• /
• v.16 no.4
• /
• pp.533-541
• /
• 2005

Non-aqueous cleaning agents were formulated with 2,2,2-trifluoroethanol (TFEA) and hydrofluoroether (HFE), and their physical properties and cleaning abilities were examined. TFEA-based aqueous cleaning agents were also formulated with nonionic surfactants, hydrotropes and builders, and their cleaning abilities were compared. Possibilities of these cleaning agents as substitutes for CFC-113 and 1,1,1-TCE were finally evaluated. In this work, fluxes, cutting oils, greases, and fluoric oils were selected as model contaminants for cleaning experiments. These contaminants have different properties of water-solubility or hydrophilicity, and fat-solubility or lypophilicity. Cleaning abilities of TFEA-based cleaning agents were analyzed and compared through the measurement of contaminant weight changes as a function of cleaning time, and their possibilities as alternative cleaning agents were evaluated. As a result, it was shown that TFEA and HFE-based non-aqueous cleaning agents have quite a good cleaning power for fluxes and fluorine soils but low one for greases. And TFEA-based agueous cleaning agents which consisted of nonionic surfactants, hydrotrope, and builders were very effective for cleaning fluxes and greases under certain formulation conditions. Thus, it was revealed that the TFEA-based cleaning agents were very effective for cleaning specific contaminants and can be used as substitutes for CFC-113 and 1,1,1-TCE in some industrial applications.

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.15 no.5
• /
• pp.92-100
• /
• 2011

This paper describes a numerical modeling for deflection calculation using the natural frequency response that is measured acceleration response for concrete bridges. In the formulation of the dynamic deflection, the change amounts and the transformed responses about six kinds of free vibration responses are defined totally. The predicted response can be obtained from the measured acceleration data without requiring the knowledge of the initial velocity and displacement information. The relationship between the predicted response and the actual deflection is derived using the mathematical modeling that is induced by the process of a acceleration test data. In this study, in order to apply the proposed response predicted model to the integration scheme of the natural frequency domain, the Fourier Fast Transform of the deflection response is separated into the frequency component of the measured data. The feasibility for field application of the proposed calculation method is tested by the mode superposition method using the PSC-I bridges superstructures under several cases of moving load and results are compared with the actually measured deflections using transducers. It has been observed that the proposed method can asses the deflection responses successfully when the measured acceleration signals include the vehicle loading state and the free vibration behavior.

• Food Science of Animal Resources
• /
• v.40 no.6
• /
• pp.1014-1032
• /
• 2020

This work aimed to investigate the psychobiotic effects of six bacterial strains on the mind and behavior of male Wistar rats. The probiotic (PRO) group (n=7) were rats pre-treated with antibiotics for 7 days followed by 14-day probiotic administration, antibiotics (ANT) group (n=7) were rats treated with antibiotics for 21 days without probiotics. The control (CON) group (n=7) were rats that received sham treatment for 21 days. The six bacterial strains with probiotic properties were mostly isolated from Thai fermented foods; Pedicoccus pentosaceus WS11, Lactobacillus plantarum SK321, L. fermentum SK324, L. brevis TRBC 3003, Bifidobacterium adolescentis TBRC 7154 and Lactococcus lactis subsp. lactis TBRC 375. The probiotics were freeze-dried into powder (6×10⁹ CFU/5 g) and administered to the PRO group via oral gavage. Behavioral tests were performed. The PRO group displayed significantly reduced anxiety level and increased locomotor function using a marble burying test and open field test, respectively and significantly improved short-term memory performance using a novel object recognition test. Antibiotics significantly reduced microbial counts in rat feces in the ANT group by 100 fold compared to the PRO group. Probiotics significantly enhanced antioxidant enzymatic and non-enzymatic defenses in rat brains as assessed using catalase activity and ferric reducing antioxidant power assay, respectively. Probiotics also showed neuroprotective effects with less pyknotic cells and lower frequency of vacuolization in cerebral cortex. This multi-strain probiotic formulation from Thai fermented foods may offer a potential to develop psychobiotic-rich functional foods to modulate human mind and behaviors.

• Journal of the Economic Geographical Society of Korea
• /
• v.23 no.1
• /
• pp.56-70
• /
• 2020

This study identifies the impact of social network service (SNS) on the spatial characteristics of retail stores locations in the hyper-connected society, which have been closely related to the everyday lives of urban residents. In particular, we focus on the changes in the spatial distribution of restaurants since the information retrieval process was added to the decision-making process of a consumer's restaurant selection. Empirically, we analyze restaurants in Seoul, Korea since the smart-phone was introduced. By applying the kernel density estimation and Moran's I index, we examine the changes in the spatial distribution pattern of restaurants during the last ten years for running, newly-open and closed restaurants as well as SNS popular ones. Finally, we develop a spatial regression model to identify geographic features affecting their locations. As the results, we identified geographical variables and online factors that influence the location of restaurants. The results of this study could provide important groundwork for food and beverage location planning and policy formulation.

• Geophysics and Geophysical Exploration
• /
• v.12 no.4
• /
• pp.361-366
• /
• 2009

Layered-earth Green's functions in electormagnetic (EM) surveys play a key role in modeling the response of exploration targets. They are computed through the Hankel transforms of analytic kernels. Computational precision depends upon the choice of algebraically equivalent forms by which these kemels are expressed. Since three-dimensional (3D) modeling can require a huge number of Green's function evaluations, total computational time can be influenced by computational time for the Hankel transform evaluations. Linear digital filters have proven to be a fast and accurate method of computing these Hankel transforms. In EM modeling for 3D inversion, electric fields are generally evaluated by the secondary field formulation to avoid the singularity problem. In this study, three components of electric fields for five different sources on the surface of homogeneous half-space were derived as primary field solutions. Moreover, reflection coefficients in TE and TM modes were produced to calculate EM responses accurately for a two-layered model having a sea layer. Accurate primary fields should substantially improve accuracy and decrease computation times for Green's function-based problems like MT problems and marine EM surveys.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.43 no.12
• /
• pp.1959-1963
• /
• 2014

The feasibility of incorporating flaxseed powder up to 8% (w/w) as a value-added food ingredient in convenient food products was investigated using pound cake as a model system. The moisture content, volume, height, and hardness of cakes decreased significantly with increasing levels of flaxseed powder (P<0.05). On the other hand, baking loss increased with higher flaxseed powder in the formulation (P<0.05). Lightness and yellowness decreased, whereas redness increased significantly (P<0.05). 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical scavenging activities increased significantly (P<0.05), and they were well correlated. The consumer acceptance test indicated that addition of 2% flaxseed powder had the most favorable effect on consumer preferences in general. Based on the overall observation, pound cakes with 2% flaxseed powder is recommended to take advantage of its functional properties without sacrificing consumer acceptability.

• Geophysics and Geophysical Exploration
• /
• v.5 no.2
• /
• pp.84-92
• /
• 2002

In this study, the new modeling scheme has been developed for recently designed and tested electromagnetic survey, which adapts horizontal magnetic dipole with $1\;kHz\~1\;MHz$ frequency range as a source. The 2.5-D secondary field formulation in wavenumber domain was constructed using finite element method and verified through comparing results with layered-earth solutions calculated by integral equations. 2-D conductive- and resistive-block models were constructed for calculating electric field, magnetic field and impedance - the ratio of electric and magnetic fields which are orthogonal each other. This study showed that electric field and impedance are superior in identifying 2-D isolated-body model to magnetic field. In particular, impedance gives more stable results than electric field with similar spatial resolving power, because electric field is divided by magnetic field in impedance. Thus the impedance analysis which uses electric and magnetic fields together would give better result in imaging the shallow anomalies than conventional EM method.