• Transactions of the Korean Society of Mechanical Engineers A
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• v.41 no.5
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• pp.409-414
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• 2017

In the thin strip coiling process, it is necessary to use a sleeve with a mandrel to prevent excessive deformation of the strip. The stress distribution in the sleeve and strip is an important factor to determine the size of the sleeve. However, an experimental approach is almost impossible because of the accumulation of high pressure. A finite element (FE) model of the strip coiling process was developed in this study. Then, the radial and hoop stresses on the sleeve and strip were investigated using FE analyses. The theoretical values and analysis results under idealized conditions were compared to verify the FE model. The effect of the strip thickness on the stress distribution was also investigated. The radial stress increased by 6.3 times for a 1-mm-thick strip at the coil starting point. The radial stress at the sleeve increased by 14.8 % with a stacked thickness of 90 mm because of the reaction force applied by the mandrel.

• The Transactions of the Korean Institute of Electrical Engineers B
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• v.51 no.11
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• pp.601-611
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• 2002

As resent trends in structural construction have been to build taller and larger structures than any time in the past, they have had high flexibility and low damping that can cause large vibration response under severe environmental loading such as earthquakes, winds, and mechanical excitations. The damper with mass and sqring is one aproach to safeguarding the structure against excessive vibrations. In this paper, a large capacity hybrid-type linear motor damper(LMD) was designed and fabricated for the application to the vibration control of a large building structure model. It has been designed to be able to move the damper mass, 1,500 kg up to ${\pm}250mm$ strokes at the first mode natural frequency of the building structure model, ${\pm}0.51Hz$. Linear motor is consisted of the fixed coil and the movable NdFeB permanent magnets field part. The PM field part composed magnet modules and iron yoke, is the damper mass itself, 1500kg. LMD therefore has a simplified structure and requires a few elements in the driving system, being compared with a rotary motor damper and a hydraulic damper. However, the manufacture of large PM linear actuator is difficult because of the limit of PM size and the attraction and repulsion at the assembly of PM. Therefore, large damper system is manufactured and tested for dynamic characteristics and frequency response.

• Proceedings of the KIEE Conference
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• 2007.07a
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• pp.38-39
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• 2007

Recently, utility network is becoming more and more complicated and huge due to IT(Information Technology) and OA(Office Automation) devices. In addition to, demands of power conversion devices which have non-linear switching devices are getting more and more increased. Voltage sag from sudden increasing loads is also one of the major problems inside of the utility network. In order to compensate the voltage sag problem, power compensation devices systems could be a good solution method. In case of voltage sag, it needs an energy source to overcome the energy caused by voltage sag. Superconducting Magnet Energy Storage (SMES) is a very good promising source due to the high response time of charge and discharge. This paper presents a real-time simulation algorithm for the SMES by using Real Time Digital Simulator (RTDS). With this algorithm users can easily do the simulation of utility power network applied by SMES system with the SMES coil modeled in RTDS.

• Journal of Biomedical Engineering Research
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• v.43 no.4
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• pp.193-198
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• 2022

Contrast enhanced magnetic resonance imaging using gadolinium-based contrast agent (GBCA) is a very useful in vivo technique to visualize the inner ear pathology including endolymphatic hydrops. Although systemic intravenous (IV) administration can visualize the perilymph space, the visualization was possible by indirect passage of contrast agent through blood-perilymph barrier. All animal experimental procedures were performed under anesthesia with 5% isoflurane. Lipopolysaccharide (LPS) was instilled into the left tympanic cavity through the tympanic membrane using a sterile 27gauge needle to induce hydrops model. Tucker-Davis Technologies system was used to measure Auditory Brainstem Responses (ABRs). For intracerebroven-tricular (ICV) administration, 25 µmol of GADOVIST (Bayer, Berlin, Germany) was used and diluted GADOVIST injection was 10 µl. MR imaging was acquired with a 9.4 Tesla MRI scanner. Transmit-receive volume coil with 40 mm inner diameter and 75 mm out diameter was used. ICV administration well demonstrated the strong enhancement along the cerebrospinal fluid (CSF) microcirculation pathway including CSF fluid in the subarachnoid space and CSF space of the inner ear structures. On the other hand, IV administration showed no contrast enhancement along the CSF microcirculation pathway and showed weak enhancement in the inner ear structures. In case of rat hydrops model, ICV administration showed that the reduced contrast enhancement in the perilymph space of the hydrops induced inner ear compared to the contrast enhancement in the perilymph space of the normal inner ear. New systemic ICV administration method provide contrast enhancement of GBCA in the inner ear through CSF microcirculation pathway.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2001.06a
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• pp.149-153
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• 2001

To investigate the mode of bactericidal action for antimicrobial peptide, pediocin, synthetic and mutant pediocins were prepared by direct chemical synthesis. Native pediocin was purified from Pedio-coccus acidilactici M and its conformational structure and bactericidal functions were analyzed and compared to synthetic pediocin. Schematic mode of pediocin actions, how pediocin binds on the target cell membrane, penetrates and makes tunnel are proposed. For these purposes, primary and secondary structures of pediocin was analyzed and disulfide bond assignment was also done. The pediocin purified from P. acidilactici M had high effective bactericidal ability against gram positive bacteria, especially Listeria monocytogenes and was very stable at extreme pHs and even at high temperatures such as autoclaving temperature (121$^{\circ}C$). Pediocin was consisted of 44 amino acids with four cysteines. Novel synthetic peptides were achieved by solid phase peptide synthesis(SPPS) method. To explain the function of cysteine in C-terminal region, mutant pediocin, Ped[C24A＋C44A], was synthesized and their structural and biological functions were analyzed. Second mutant pediocin, Ped[KllE], was prepared to explain the function of lysine at 11 of N-terminal part of pediocin, especially loop of $\beta$-sheet, and to predict the initial binding site of pediocin. The native and synthetic pediocins was showed random coil conformation by spectropolarimetry in moderate conditions. This conformation was observed in extreme conditions such as high temperature and low and high pHs, also. Circular dichroism(CD) data also showed the existence of $\beta$-turn structure in N-terminal part both native and synthetic pediocins. A structural model for pediocin predicts that 18 amino acids in the N-terminal part of the peptide assume a three-strand $\beta$-sheet conformation. This random coil in C-terminal part of pediocin was converted to folding structure, helix structure, in nonpolar solvents such as alcohol and TFE. The disulfide bond between $^{9}$ Cys and $^{14}$ Cys was concrete and inevitable, however, evidences of disulfide bond between $^{24}$ Cys and $^{44}$ Cys was not. Data of Ped[C24A＋C44A], pediocin mutant showed that $^{44}$ Cys was required during killing the target cells but not inevitable, since Ped[C24A＋C44A] still have bactericidal activity but much less than native pediocin. Another pediocin mutant, Ped[KllE], had still bactericidal activity, was controversial to propose that positive charge like as $^{11}$ Lys in loop or hinge in bacteriocin bound or helped to binding to microorganism with electrostatic interaction between cell membrane especially teichoic acid and positive amino acid nonspecifically. The conformation of pediocin among native, synthetic and mutant pediocins did not show big difference. The conformations between oxidized and reduced pediocin were almost similar regardless of native or synthetic.

• Proceedings of the Acoustical Society of Korea Conference
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• autumn
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• pp.233-236
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• 2001

일반적으로 스피커를 동작시키게 되면, 스피커 보이스 코일에 열이 발생하게 되고, 열에 의한 보이스코일의 저항의 증가가 나타나게 되는데, 기존의 연구는 저음용 스피커(Woofer) 만을 대상으로 스피커의 음향특성 변화들에 관해 수행되었다. 그러나, 현대의 스피커는 A/V시스템분야의 발전에 따라 고음재생의 충실도가 강조되고 있는 경향이므로, 고음용 스피커(Tweeter)에 대한 열 발생의 영향을 조사하였다. 본 연구에서는 고음용 스피커의 입력전력에 따른 특성 변화를 조사하고, 입력전력의 증가로 인한 보이스코일(voice coil)의 저항 증가에 따른 고음용 스피커의 특성변화에 관해 실험하였다. 그리고, 스피커의 진동에 의한 펌프(pump) 역할을 이용하는 냉각용 구멍을 뚫어 스피커의 특성변화를 검토하였다. 실험대상으로는 직경 25mm의 돔(Dome)형 진동판을 가진 고음용 스피커와 이 스피커의 후면 중심부에 구멍을 뚫어 열 방출구가 형성된 3종의 시료를 대상으로 특성변화를 비교$\cdot$측정하였다. 여기에서, 사용된 시료는 국내 Y사의 판매용 고음용 스피커로 제품의 원 상태인, 구멍이 없는 것을 기준시료로 하고, 이와 동일한 제품들의 후면에 각각 직경 5mm, 10mm, 15mm의 구멍을 가공하여 비교시료로 하였다. 기준 및 비교시료의 스피커 특성을, 한국산업규격 KS C 6027의 측정법에 따라, 입력 1W 상태에서 기준시료의 사양을 측정하였고[1], 입력을 0.5W, 1W, 2W, 4W, 8W, 16W로 가하여, 시료별 입력증가에 따른 스피커의 주파수 응답특성, 임피던스(Impedance), 조화 왜(Harmonic Distortion)의 변화율 측정을 통해 스피커의 특성변화 정도를 검토하였다. 향후, 본 연구의 결과는 고음용 스피커의 특성 열화에 대한 예측 및 개선 방안을 제시하는 기본 자료로 활용이 가능할 것으로 사료된다.용하여 현금흐름예측을 할 수 있는 Model을 제시하였다. 특히 건설공사의 현금흐름 예측의 중요한 요소인 Cash-Out에 대하여, 공사비 구성요소인 자재, 노무, 중기, 외주, 경비등 각 Resource의 보할(Weights)을 실 공사원가에 따른 보할의 변화와 Resource들의 Time Lag를 적용 기존 연구자의 Model과 다른 Model을 제시하였다. 또한 기존 연구자들의 Model과 비교하여 편리성, 정확도 및 신뢰성이 높은 Model임도 증명하였다.세대까지도 발현수준이 유지될 것으로 판단된다. 이러한 연구결과는 계통으로 확립된 형질전환 동물에 부여된 새로운 유전형질은 지속적으로 후대로 유전될 수 있음을 제시한다.잖⨀瘀Ā퀇Āゑ잖⨀Ā퀇Ԁ￿™잖⨀䌀Ā퀇ĀꄏĀꀏꄏĀꀏ₱?⨀Ā Ԁ￿䂱?⨀ऀĀ耀Ā삱?⨀؀Ā Ā?⨀ጀĀ耀Ā?돀ꢘ?⨀硩?⨀ႎ?⨀?⨀넆돐쁖잖⨀쁖잖⨀／ࠐ?⨀焆덐瀆倆Āⶇ퍟ⶇ퍟ĀĀĀĀ磀鲕좗?⨀肤?⨀⁅Ⴅ?⨀쀃잖⨀䣙熸ጁ↏?⨀

• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.3
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• pp.645-661
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• 2009

The water extract of Dohongsamul-Tang(DHSMT) has been traditionally used to stroke and brain injuries in Oriental Medicine. The present study was designed to investigate the effects of DHSMT on the gene expression profile of cerebral infarction by cDNA microarray in photothrombotic ischemia mouse model. Photothrombotic ischemia was induced in stereotactically held male BALB/c mice using rose bengal and cold light. MRI was performed 24 hours after inducing photothrombosis using 1.5 T MRI and 47 mm surface coil to obtain T2-weighted, and contrast-enhanced images. After MRI test, animal was sacrificed and the brain sections were stained for hematoxylin and eosin and immunohistochemistry. MRI and histological analysis revealed that lesion of thrombotic ischemia was well induced in the cortex with the evidence of biological courses of infarction. The target area of thrombotic infarction was 1 mm anterior to bregma and 3 mm lateral to midline with 2 mm in diameter, which were decreased by administration of DHSMT. To assess gene expression pattern of cerebral infarction, mRNA was isolated and reacted with microarray chip(Agilant's DNA Microarray 44K). Scatter and MA plot analysis were performed to clustering of each functional genes. M value [M=log2(R/G), A={log2(R ${\times}$ G)}/2] was between -0.5 and +0.5 with 40% difference. After pretreatment with DHSMT, the expression levels of mRNA of many genes involved in various signaling pathway such as apoptosis, cell cycle, cell proliferation, response to oxidative stress, immune response, angiogenesis, and inflammatory cytokine were markedly inhibited in photothrombotic ischemia lesion compared to the control group. These results suggest that DHSMT prevent ischemic death of brain on photothrombotic ischemia model of mice through modulation of gene expression at the transcriptional level.

• 한국균학회소식:학술대회논문집
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• 2015.05a
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• pp.61-61
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• 2015

Rice blast, caused by the fungal pathogen Magnaporthe oryzae, is one of the most destructive diseases of rice worldwide. The rice - M. oryzae pathosystem has become a model in the study of plant - fungal interactions due to its economic importance and accumulating knowledge. During the evolutionary arms race with M. oryzae, rice plants evolved a repertoire of Resistance (R) genes to protect themselves from diseases in a gene-for-gene fashion. M. oryzae secretes a battery of small effector proteins to manipulate host functions for its successful infection, and some of them are recognized by host R proteins as avirulence effectors (AVR), which turns on strong immunity. Therefore, the analysis of interactions between AVRs and their cognate R proteins provide crucial insights into the molecular basis of plant - fungal interactions. Rice blast resistance genes Pik, Pia, Pii comprise pairs of protein-coding ORFs, Pik-1 and Pik-2, RGA4 and RGA5, Pii-1 and Pii-2, respectively. In all three cases, the paired genes are tightly linked and oriented to the opposite directions. In the AVR-Pik/Pik interaction, it has been unraveled that AVR-Pik binds to the N-terminal coiled-coil domain of Pik-1. RGA4 and RGA5 are necessary and sufficient to mediate Pia resistance and recognize the M. oryzae effectors AVR-Pia and AVR1-CO39. A domain at the C-terminus of RGA5 characterized by a heavy metal associated domain was identified as the AVR-binding domain of RGA5. Similarly, physical interactions among Pii-1, Pii-2 and AVR-Pii are being analyzed.

• Journal of the Korean Society for Railway
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• v.19 no.3
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• pp.324-330
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• 2016

A magnetic contactor is a switching device widely used for electric circuits. For the operation of magnetic contactors, magnetic coils are essential; these coils create and interrupt the electric circuit. In this paper, the finite element analysis model was developed to reflect the experimental data, and was verified through alteration of the applied voltages and the numbers of turns. Effects of electromagnetic force on the geometrical variations of the facing poles for fixed and moving cores of two magnet coils were investigated. In addition, effects of slope and air gap size between two facing poles on the electromagnetic force were explored through the distribution of the magnetic flux density in the magnetic coils of a push-type solenoid. Through this analysis, the characteristics of the electromagnetic force against the facing poles were explored.

• Journal of Microbiology and Biotechnology
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• v.27 no.4
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• pp.775-784
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• 2017

A neutral xylanase (CcXyn) was identified from Coprinus cinereus. It has a single GH10 catalytic domain with a basic amino acid-rich extension (PVRRK) at the C-terminus. In this study, the wild-type (CcXyn) and C-terminus-truncated xylanase ($CcXyn-{\Delta}5C$) were heterologously expressed in Pichia pastoris and their characteristics were comparatively analyzed with aims to examine the effect of this extension on the enzyme function. The circular dichorism analysis indicated that both enzymes in general had a similar structure, but $CcXyn-{\Delta}5C$ contained less ${\alpha}-helices$ (42.9%) and more random coil contents (35.5%) than CcXyn (47.0% and 32.8%, respectively). Both enzymes had the same pH (7.0) and temperature ($45^{\circ}C$) optima, and similar substrate specificity on different xylans. They all hydrolyzed beechwood xylan primarily to xylobiose and xylotriose. The amounts of xylobiose and xylotriose accounted for 91.5% and 92.2% (w/w) of total xylooligosaccharides (XOS) generated from beechwood by CcXyn and $CcXyn-{\Delta}5C$, respectively. However, truncation of the C-terminal 5-amino-acids extension significantly improved the thermostability, SDS resistance, and pH stability at pH 6.0-9.0. Furthermore, $CcXyn-{\Delta}5C$ exhibited a much lower $K_m$ value than CcXyn (0.27 mg/ml vs 0.83 mg/ml), and therefore, the catalytic efficiency of $CcXyn-{\Delta}5C$ was 2.4-times higher than that of CcXyn. These properties make $CcXyn-{\Delta}5C$ a good model for the structure-function study of $({\alpha}/{\beta})_8$-barrel-folded enzymes and a promising candidate for various applications, especially in the detergent industry and XOS production.