Journal of Biomedical Engineering Research (대한의용생체공학회:의공학회지)

The Korean Society of Medical and Biological Engineering (대한의용생체공학회)
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Enhancement Characteristics of Gadolinium Contrast Agent in the Rat Inner Ear Perilymph through CSF microcirculation

뇌척수액 미세순환을 통한 래트 내이 외림프의 가돌리늄 조영제 증강 특성

  • Kim, Min Jung (Medical Device Development Center, KBIO Osong Medical Innovation Foundation) ;
  • Lee, Sang-Yeol (Department of Medical & Biological Engineering, Kyungpook National University) ;
  • Lee, Hui Joong (Department of Radiology, Kyungpook National University School of Medicine) ;
  • Lee, Taekwan (Korea Brain Research Institute) ;
  • Chang, Yongmin (Department of Medical & Biological Engineering, Kyungpook National University)
  • 김민정 (오송첨단의료산업진흥재단 첨단의료기기개발지원센터) ;
  • 이상열 (경북대학교 대학원 의용생체공학과) ;
  • 이희중 (경북대학교 의과대학 영상의학교실) ;
  • 이태관 (한국뇌연구원 첨단뇌연구장비센터) ;
  • 장용민 (경북대학교 대학원 의용생체공학과)
  • Received : 2022.06.30
  • Accepted : 2022.07.18
  • Published : 2022.08.31
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Abstract

Contrast enhanced magnetic resonance imaging using gadolinium-based contrast agent (GBCA) is a very useful in vivo technique to visualize the inner ear pathology including endolymphatic hydrops. Although systemic intravenous (IV) administration can visualize the perilymph space, the visualization was possible by indirect passage of contrast agent through blood-perilymph barrier. All animal experimental procedures were performed under anesthesia with 5% isoflurane. Lipopolysaccharide (LPS) was instilled into the left tympanic cavity through the tympanic membrane using a sterile 27gauge needle to induce hydrops model. Tucker-Davis Technologies system was used to measure Auditory Brainstem Responses (ABRs). For intracerebroven-tricular (ICV) administration, 25 µmol of GADOVIST (Bayer, Berlin, Germany) was used and diluted GADOVIST injection was 10 µl. MR imaging was acquired with a 9.4 Tesla MRI scanner. Transmit-receive volume coil with 40 mm inner diameter and 75 mm out diameter was used. ICV administration well demonstrated the strong enhancement along the cerebrospinal fluid (CSF) microcirculation pathway including CSF fluid in the subarachnoid space and CSF space of the inner ear structures. On the other hand, IV administration showed no contrast enhancement along the CSF microcirculation pathway and showed weak enhancement in the inner ear structures. In case of rat hydrops model, ICV administration showed that the reduced contrast enhancement in the perilymph space of the hydrops induced inner ear compared to the contrast enhancement in the perilymph space of the normal inner ear. New systemic ICV administration method provide contrast enhancement of GBCA in the inner ear through CSF microcirculation pathway.

Keywords

Acknowledgement

본 연구는 한국연구재단의 뇌연구 과제(NRF-2018M3C7A1053217)와 과학기술정보통신부 한국뇌연구원 기관고유사업(21-BR-05-01)에 의해 지원을 받아 수행하였음.

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