• The Journal of Korean Physical Therapy
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• 제22권3호
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• pp.71-77
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• 2010

Purpose: To determine whether upregulation of inducible nitric oxide synthase (iNOS) transcription and translation is related to radicular pain in a model of lumbar disc herniation. Also, to investigate the temporal changes of mRNA expression of iNOS and the identity of iNOS and transient receptor potential vanilloid (TRPV) 1 channel expression cells in dorsal root ganglion (DRG) of a model of lumbar disc herniation. Methods: A lumbar disc herniated rat model was developed by implantation of the autologous nucleus pulposus, harvested from the coccygeal vertebra of each tail, on the left L5 nerve root just proximal to the DRG. Rats were tested for mechanical allodynia of the plantar surface of both hind paws 2 days before surgery and 1, 5, 10, 20 and 30 days postoperatively. Reverse transcription polymerase chain reaction (RT-PCR) was used to follow iNOS mRNA expression. To stain iNOS and TRPV1 in DRG, an immunohistochemical study was done 10 days after surgery. Results: A significant drop in mechanical withdrawal threshold on the ipsilateral and contralateral hind paws was observed 1 day after surgery and was prolonged to 30 days in rats with lumbar disc herniation. The expression of mRNA for iNOS peaked at postoperative day 10 on both sides of the DRG. iNOS-positive sensory neurons in the DRG varied in size from large to small diameter cells. A majority of small and intermediate sensory neurons were TRPV1-positive cells. Double immunofluorescence staining for TRPV1 and iNOS revealed that most intermediate TRPV1-positive sensory neurons co-localized with iNOS-positive neurons. Conclusion: Nucleus pulposus-induced mechanical allodynia can be generated without mechanical compression. This pain is related to temporal changes in expression of iNOS mRNA in the DRG. Co-localization of TRPV1 and iNOS in intermediate neurons of the DRG is correlated with pain modality and intensity.

• 대한의용생체공학회:의공학회지
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• 제25권1호
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• pp.71-76
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• 2004

척추 측만증 교정 시 유합 범위에 따른 수술 후 의 효과를 분석하기 위하여 King-Moe type II 형태의 척추 측만증 환자를 대상으로 이에 대한 수학적 유한 요소 모델을 개발하였다. 유한 요소 모델을 이용하여 척추경 나사못을 이용한 고정 및 강봉 감염술 형태의 수술모사를 수행하였으며, 유합 범위를 각기 달리하여 수술 시뮬레이션 후의 척추계의 변화 즉 Cobb 각도, 첨추체 축회전, 흉추 후만각 그리고 늑골고의 변화를 정량적으로 계산하였다. 해석 결과 강봉 감염술의 경우에서 보다 강봉을 척추경 나사못에 연결시키는 과정에서 훨씬 더 많은 Cobb각도의 변화를 얻을 수 있었다. 강봉 감염술 과정에서는 약간의 Cobb각도 감소를 유발시킬 수 있었으나 오히려 척추체 축회전과 늑골 돌출고의 증가를 나타내었다 아울러 강봉과 척추경 나사못의 체결 과정이 척추 후만각 유지에도 큰 역할을 하고 있음을 알 수 있었다. 유합 범위를 변곡 발생 추체의 위치 보다 한 단계씩 길게(T4-12) 하여 60o의 강봉 감염술을 시행하였을 경우 Cobb 각도를 두 배 감소시킬 수 있었으나 급격한 늑골고와 첨추체 축회전량의 증가가 유발되었다. 해석 결과 유합 범위는 만곡 흠추체에서 변곡이 발생되는 위치 보다 한 레벨씩 작게 선정하는 것이 최적의 유합 범위임을 알 수 있었다.

• 한국비블리아학회지
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• 제33권4호
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• pp.73-92
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• 2022

본 연구는 기존 국립세종도서관 정책정보포털(POINT)의 국정과제 서비스를 뛰어넘는 고품질 정책정보서비스 제공을 위하여, 새로운 국정과제 이행에 필요한 정책자료를 효과적으로 서비스할 수 있는 모형을 개발하고자 한다. 이에 개별 접근을 통한 데이터 검색 대신 통합된 검색 환경을 제공하는 것이 필요하며, 이를 위한 국정과제서비스 모형 구축을 4가지 모형으로 제안하였다. 첫째, 국정과제서비스 시스템 운영 부문으로 인터페이스 측면, 전산 시스템 구상, 전자정보원 측면, 전산 시스템 구상을 제안하였다. 둘째, 국정과제서비스 정보원 부문으로 Linked Open Data 시스템 구상, 정책정보 시계열 서비스 시스템 구상, 국외 원문자료 번역서비스, 연구자 희망자료 확충을 제시하였다. 셋째, 국정과제서비스 홍보 부문으로 정책 이용자를 위한 홍보 강화, 사이트 브랜드 구축 및 홍보, SNS 채널 운영, 보조자료 활성화 강화, 외부 서비스에서의 접근성 강화를 제안하였다. 넷째, 국정과제서비스 협업 및 연계 부문으로 Open API, 클라우드 서비스, 해외도서관과의 정보 네트워크 확장을 제안하였다.

• Archives of Pharmacal Research
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• 제20권5호
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• pp.404-409
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• 1997

Trout CYP1A-CAT expression construct was generated by cloning -3.5 Kb $5^I$ flanking DNA of trout liver CYP1A gene in front of CAT gene at pCAT-basic vector. Hepa 1 cells, which are known to contain a functional arylhydrbcarbon $receptor^I$ were transfected with trout CYP1A-CAT using lipofectin. 3-Methylcholanthrene (1 nM) was added into hepa 1 cells in culture in order to examine if $5^I$ flanking DNA of trout CYP1A gene could interact with mouse transactivating factors to bring about transcription of the chloramphenicol acetyltransferase(CAT) reporter gene. The level of CAT protein was measured by CAT ELISA and the level of CAT mRNA was determined by RTPCR. The treatment of 1 nM 3-methylcholanthrene resulted in two fold increases in CAT protein as well as CAT mRNA compared to untreated control hepa 1 cells. These data indicate that arylhydrocarbon receptors of mouse hepa 1 cells are functional to activate exogenously transfected trout CYP1A-CAT construct in terms of both transcription and translation of CAT. We also examined the effect of 3-methylcholanthrene on endogenous cyplal activity in hepa 1 cell. 3-Methylcholanthrene (1 nM) treatment to hepa 1 cells trahsfected with trout CYP1A-CAT construct stimulated the level of cyp1a1 mRNA by two folds and the activity of ethoxyresorufin-O-deethylase by two fold compared to that of control cells. In this study we reported that trout CYP1A-CAT reporter gene expression construct could be expressed by 3-methylcholanthrene treatment in mouse hepa 1 cells. Thus trout CYP1A-CAT could serve as a good model to study the mechanism of regulation of CYP1A1 gene expression.

• 대한간호학회지
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• 제41권3호
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• pp.325-332
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• 2011

Purpose: To develop and test the validity and reliability of the Korean version of PES-NWI measuring nursing work environments in hospitals. Methods: The Korean version of the PES-NWI was developed through forward-backward translation techniques, and revision based on feedback from focus groups. An internal consistency reliability and construct validity using confirmatory factor analysis were conducted using SPSS WIN (16.0) and AMOS (18.0). Survey data were collected from 733 nurses who worked in three acute care hospitals in Seoul, South Korea. Results: The Korean version of PES-NWI showed reliable internal consistency with a Cronbach's alpha for the total scale of .93. Factor loadings of the 29 items on the five subscales ranged from .28 to .85. The five subscales model was validated by confirmatory factor analysis (RMR<.05, CFI >.9). Conclusion: The findings of this study demonstrate that the Korean version of PES-NWI has satisfactory construct validity and reliability to measure nursing work environments of hospitals in Korea.

• Bulletin of the Korean Chemical Society
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• 제33권8호
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• pp.2508-2512
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• 2012

Cold shock proteins (CSPs) are a family of proteins induced at low temperatures. CSPs bind to single-stranded nucleic acids through the ribonucleoprotein 1 and 2 (RNP 1 and 2) binding motifs. CSPs play an essential role in cold adaptation by regulating transcription and translation via molecular chaperones. The solution nuclear magnetic resonance (NMR) or X-ray crystal structures of several CSPs from various microorganisms have been determined, but structural characteristics of psychrophilic CSPs have not been studied. Therefore, we optimized the purification process to obtain highly pure Lm-Csp and determined the three-dimensional structure model of Lm-Csp by comparative homology modeling using MODELLER on the basis of the solution NMR structure of Bs-CspB. Lm-Csp consists of a ${\beta}$-barrel structure, which includes antiparallel ${\beta}$ strands (G4-N10, F15-I18, V26-H29, A46-D50, and P58-Q64). The template protein, Bs-CspB, shares a similar ${\beta}$ sheet structure and an identical chain fold to Lm-Csp. However, the sheets in Lm-Csp were much shorter than those of Bs-CspB. The Lm-Csp side chains, E2 and R20 form a salt bridge, thus, stabilizing the Lm-Csp structure. To evaluate the contribution of this ionic interaction as well as that of the hydrophobic patch on protein stability, we investigated the secondary structures of wild type and mutant protein (W8, F15, and R20) of Lm-Csp using circular dichroism (CD) spectroscopy. The results showed that solvent-exposed aromatic side chains as well as residues participating in ionic interactions are very important for structural stability. Further studies on the three-dimensional structure and dynamics of Lm-Csp using NMR spectroscopy are required.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2000년도 하계종합학술대회 논문집(2)
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• pp.153-156
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• 2000

본 논문은 SystemC의 특징과 어떻게 SOC 설계 방법에 응용될 수 있는지 고려한다. 먼저, 기존 개발된 시스템 알고리듬을 기초로 하여 SystemC로 기능 블럭과 인터페이스를 분리하여 정의한다 이렇게 정의된 기능 블록과 인터페이스를 모듈화하고 묶어서 실행 가능한 사양을 만들어 충분한 기능 검증을 수행한다. 두번째로 S/W로 구현할 부분과 H/W로 구현할 부분을 나누어, S/W 부분의 인터페이스는 사이클 정확도를 갖도록 기술하며 기능 블럭은 기존 S/W 개발 환경을 사용하여 구현한다 H/W 부분의 IO 는 다양한 추상화단계로 이벤트를 기술하고 내부 동작은 기능에 기반을 두고 작성한다. 이 사양이 만족해야 할 시스템 요구 성능을 발휘하도록 성능분석을 수행하고, 이 결과가 S/W, H/W 분할 과정과 인터페이스 구체화 과정에 영향을 미친다. 시스템 성능을 내는 이 사양을 기초로 하여 사이클 정확도를 갖는 H/W 부분은 변환 프로그램을 이용하거나 직접 HDL RTL 설계로 변환한다. 이 방법은 기존 C/C++ 프로그램 개발자와 VHDL/Verilog 설계자가 쉽게 적응할 수 있어 기존 ASIC 개발자가 저렴한 비용으로 시스템 통합 설계 및 검증을 통하여 SoC를 개발하고자 할 때 특히 더 적합하다.

• 복식문화연구
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• 제26권1호
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• pp.82-94
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• 2018

As the number of SNS(Weibo) users in China is growing rapidly, Chinese fashion brands are heavily dependent on SNSs as a fashion marketing communication tool. For this reason, the characteristics of SNS accounts and their influences on SNS users' responses need to be studied. Thus, the present study aimed to investigate the influences of the characteristics of Chinese fashion brands' SNS accounts(Weibo) on the perceived usefulness of and satisfaction with the SNS acount, and brand loyalty. Data were collected via a questionnaire survey of men and women living in Beijing or Shanghai aged from 18 to 49 with experience of SNSs(Weibo). After a pilot survey of 70 subjects, the preliminary questionnaire was revised and then translated into Chinese. The questionnaire translated into Chinese was back-translated into Korean to ensure the translation was correct. The final questionnaire was administered to 600 subjects. Exploratory and confirmatory factor analyses, reliability analysis, and structural equation model analysis were conducted for data analysis. The results of this study were as follows: Five factors were extracted for Weibo characteristics: interaction, information provision, information recency, information reliability, and information playfulness. The information reliability, information playfulness, and interaction of SNS accounts(Weibo) had significant influences on perceived usefulness. The information playfulness, information reliability, and information recency showed significant influences on satisfaction. The perceived usefulness exerted significant influences on satisfaction and brand loyalty. The satisfaction also had statistically significant influences on brand loyalty.

• 정보처리학회논문지:소프트웨어 및 데이터공학
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• 제4권9호
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• pp.377-384
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• 2015

본 논문에서는 왜곡 제거 시계열 매칭의 시각화 도구를 제안한다. 시계열 매칭에서 왜곡 제거 지원은 정확한 유사 시계열 검색을 위한 매우 중요한 요소이다. 본 논문에서는 시계열에서 발생하는 잡음, 위치 이동, 진폭 조정, 선형 추세의 왜곡들을 이동평균 변환, 정규화 변환, 선형 추세 제거 기법을 사용하여 제거한 후, 시계열 매칭을 수행하고 그 결과를 시각화한다. 본 논문에서 제안하는 시각화 도구는 클라이언트-서버 모델을 기반으로 구현된다. 클라이언트는 사용자가 선택한 시계열의 왜곡을 제거하여 서버에 전달하고, 매칭 결과의 시각화를 수행한다. 서버는 R*-트리 기반의 다차원 인덱스로 왜곡 제거 시계열 매칭을 효율적으로 수행한다. 왜곡 제거 시계열 매칭의 결과는 다섯 가지 차트로 달리 표현하여 사용자가 직관적이고 쉽게 이해할 수 있도록 구현한다.

• 한국학교ㆍ지역보건교육학회지
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• 제20권3호
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• pp.91-100
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• 2019

Purpose: The purpose of this study was to investigate the sexual function of male patients receiving rectal cancer and to analyze the factors related to sexual function. Methods: This study included 71 male patients undergoing outpatient treatment after surgery at C University Hospital, Chonnam, Korea from April 1 to September 1, 2014. The sexual function of males with colorectal cancer was calculated using the Korean Translation of International Index of Erectile Function(IIEF). Data analysis was performed using t-test, ANOVA, and regression analysis. The study was IRB approved. Results: The sexual function index scores of the subjects were 33.28±19.47 points. Regression analysis showed that sexual function increased as the duration after operation increased(p=.001), higher location of cancer(p=.007), age decreased(p=.013). The explanatory power (adj. R2) of the analysis model was 0.186. Conclusion: Sexual function of males with rectal cancer differed according to duration after operation, and location of cancer, age. Therefore, medical staff think that it can be used as basic data for appropriate education and counseling by age, time, and type of treatment to improve sexual function of men with rectal cancer.