• 제목/요약/키워드: Mode of production

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Production of Alkaline Protease by Entrapped Bacillus licheniformis Cells in Repeated Batch Process

  • Mashhadi-Karim, Mohammad;Azin, Mehrdad;Gargari, Seyyed Latif Mousavi
    • Journal of Microbiology and Biotechnology
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    • v.21 no.12
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    • pp.1250-1256
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    • 2011
  • In this study, Bacillus licheniformis cells were immobilized by entrapment in calcium alginate beads and were used for production of alkaline protease by repeated batch process. In order to increase the stability of the beads, the immobilization procedure was optimized by statistical full factorial method, by which three factors including alginate type, calcium chloride concentration, and agitation speed were studied. Optimization of the enzyme production medium, by the Taguchi method, was also studied. The obtained results showed that optimization of the cell immobilization procedure and medium constituents significantly enhanced the production of alkaline protease. In comparison with the free-cell culture in pre-optimized medium, about 7.3-fold higher productivity was resulted after optimization of the overall procedure. Repeated batch mode of operation, using optimized conditions, resulted in continuous production of the alkaline protease for 13 batches in 19 days.

Ethanol Production by Synchronous Saccharification and Fermentation of Foodwastes

  • Han, Hyo-Jeong;Kim, Seong-Duk;Kim, Seong-Jun
    • 한국생물공학회:학술대회논문집
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    • 2005.10a
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    • pp.260-265
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    • 2005
  • In the previous research about ethanol production, we confirmed that SFW(saccharified foodwastes) medium(0.56g-ethanol/g-glucose) is mere efficient than YM medium(0.538g-ethanol/g-glucose). Ethanol production using SFW needs large enzyme cost due to the enzymatic hydrolysis of foodwastes, although the enzymes was obtained from our economical enzyme production methods, using the intact whole culture broth of Trichoderma harzianum FJ1. Therefore, in this research we used synchronous saccharification and fermentationmethod to produce ethanol using foodwastes. Ethanol production yield was 0.45g-ethanol/g-reducing sugar in synchronous saccharification and for-mentation by a fed-batch mode.

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A Dynamic Production and Transportation Model with Finite Number of Multiple Transportation Modes (유한 대수의 다종 수송수단을 고려한 동적 생산-수송 모형)

  • Sohn, Kwon-Ik
    • Journal of Industrial Technology
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    • v.29 no.A
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    • pp.183-190
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    • 2009
  • This study deals with the single-product production and transportation model with dynamic demand over finite time horizon, in which the optimal production(order) quantities, transportation modes and the number of each vehicles are determined simultaneously. The finite number of identical vehicles with capacity constraint is given to each mode. Production and transportation costs are assumed to be concave function for generality. For a relevant mathematical model formulated, the theorems and properties are discussed to present the efficient algorithm. A numerical example is solved to illustrate the algorithm developed.

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Applied Koopmanistic interpretation of subcritical prism wake physics using the dynamic mode decomposition

  • Cruz Y. Li;Xisheng Lin;Gang Hu;Lei Zhou;Tim K.T. Tse;Yunfei Fu
    • Wind and Structures
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    • v.37 no.3
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    • pp.191-209
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    • 2023
  • This work investigates the subcritical free-shear prism wake at Re=22,000 by the Koopman analysis using the Dynamic Mode Decomposition (DMD) algorithm. The Koopman model linearized nonlinearities in the stochastic, homogeneous anisotropic turbulent wake, generating temporally orthogonal eigen tuples that carry meaningful, coherent structures. Phenomenological analysis of dominant modes revealed their physical interpretations: Mode 1 renders the mean-field dynamics, Modes 2 describes the roll-up of the Strouhal vortex, Mode 3 describes the Bloor-Gerrard vortex resulting from the Kelvin-Helmholtz instability inside shear layers, its superposition onto the Strouhal vortex, and the concurrent flow entrainment, Modes 6 and 10 describe the low-frequency shedding of turbulent separation bubbles (TSBs) and turbulence production, respectively, which contribute to the beating phenomenon in the lift time history and the flapping motion of shear layers, Modes 4, 5, 7, 8, and 9 are the relatively trivial harmonic excitations. This work demonstrates the Koopman analysis' ability to provide insights into free-shear flows. Its success in subcritical turbulence also serves as an excellent reference for applications in other nonlinear, stochastic systems.

Certification as a Mode of Governance ISO and CMM in Offshore st. Petersburg

  • Feakins, Melanie
    • Proceedings of the KGS Conference
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    • 2003.11a
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    • pp.106-114
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    • 2003
  • The globalisation of software production processes is a maze of connected phenomena with dramatically increasing importance as the development of W related industries and services escalates on a global scale. While commercial and industrial software production and related services are historically associated with advanced industrial economies of the North & West, contemporary changes of the past decade suggest that the industry is developing rapidly on a global scale incorporating countries that have historically been classified as '2nd and 3rd world' or periphery and semi-periphery by different theoretical approaches. (omitted)

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Predicting Methane Production Potential of Anaerobic Co-digestion of Swine Manure and Food Waste

  • Shin, Joung-Du;Han, Sung-Su;Eom, Ki-Cheol;Sung, Shi-Hwu;Park, Sang-Won;Kim, Hyun-Ook
    • Environmental Engineering Research
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    • v.13 no.2
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    • pp.93-97
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    • 2008
  • Anaerobic co-digestion of swine manure and food waste for biogas production was performed in serum bottles at 2% volatile solids(VS) concentration and various mixing ratios of two substrates(swine manure: food waste = 100 : 0 $\sim$ 0 : 100). Through kinetic mode of surface methodology, the methane production was fitted to a Gompertz equation. The specific methane production potential of swine manure alone was lower than that of food waste. However, maximum methane production potential increased up to 1.09-1.22% as food waste composition increased up to the 80%. The maximum methane production value of food waste was 544.52 mL/g VS. It was observed that the maximum methane production potential of 601.86 mL/g VS was found at the mixing ratio of 40:60.

Recent Development of Optically Compensated Bend (OCB) Mode TFT-LCD

  • Wakemoto, Hirofumi;Nishiyama, Kazuhiro;Nakao, Kenji;Takimoto, Akio
    • 한국정보디스플레이학회:학술대회논문집
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    • 2005.07a
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    • pp.562-566
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    • 2005
  • Toshiba Matsushita Display Technology (TMD) has firstly succeeded in mass production of OCB (Optically Compensated Bend) mode liquid crystal display panels which have excellent moving picture quality almost the same as CRT. We have newly developed 32 -inch diagonal HD ($1366{\times}768\;pixels$) panels using OCB mode and low temperature p-Si TFT (LTPS) array substrates. High performance of brightness of $600cd/m^2$ and contrast ratio of 600 : 1 was obtained by using pseudo-impulse driving method to insert a black period between continuous two frames, and also by using blinking backlight method. Furthermore, moving picture response time (MPRT) 6.5ms has been obtained by optimization of black insert driving and backlight blinking, without the great sacrifices of contrast ratio and luminance.

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The fermentation kinetics of protease inhibitor production by streptomyces fradiae (Streptomyces fradiae에서 분리한 단백질 분해효소 저해물질 생성의 동력학적 특성)

  • 이병규;정영화;이계준
    • Korean Journal of Microbiology
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    • v.28 no.3
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    • pp.264-267
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    • 1990
  • The objectives of the current studies were to establish the optimal conditions for the production of extracellular protease inhibitor in a strain of Streptomyces fradiae. As results, it was found that cell specific growth rate was very critical for the production of protease inhibitor and the optimum specific growth rate was found to be 0.05 h$^{-1}$ . Dissolved oxygen tension and pH were also important to regulate the inhibitor production. The inhibitory mode of the purified inhibitor to .alpha.-chymotrypsin was found to be competitive (K$_{i}$=5.5*10$^{-7}$ M). One mole of inhibitor could bind two moles of .alpha.-chymotrypsin and the complex has very low dissociation constant.t.

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Solid Oxide Fuel Cells for Power Generation and Hydrogen Production

  • Minh, Nguyen Q.
    • Journal of the Korean Ceramic Society
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    • v.47 no.1
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    • pp.1-7
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    • 2010
  • Solid oxide fuel cells (SOFCs) have been under development for a variety of power generation applications. Power system sizes considered range from small watt-size units (e.g., 50-W portable devices) to very large multi-megawatt systems (e.g., 500-MW base load power plants). Because of the reversibility of its operation, the SOFC has also been developed to operate under reverse or electrolysis mode for hydrogen production from steam (In this case, the cell is referred to as solid oxide electrolysis cell or SOEC.). Potential applications for the SOEC include on-site and large-scale hydrogen production. One critical requirement for practical uses of these systems is long-term performance stability under specified operating conditions. Intrinsic material properties and operating environments can have significant effects on cell performance stability, thus performance degradation rate. This paper discusses potential applications of the SOFC/SOEC, technological status and current research and development (R&D) direction, and certain aspects of long-term performance degradation in the operation of SOFCs/SOECs for power generation/hydrogen production.

The Improvement of Cephalosporin C Production by Fed-batch Culture of Cephalosporium acremonium M25 Using Rice Oil

  • Kim Jin Hee;Lim Jung Soo;Kim Seung Wook
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.9 no.6
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    • pp.459-464
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    • 2004
  • The objective of this study is to improve cephalosporin C (CPC) production byoptimization of medium and culture conditions. A statistical method was introduced to optimize the main culture medium. The main medium for CPC production was optimized using a statistical method. Glucose and corn steep liquor (CSL) were found to be the most effective factors for CPC production. Glucose and CSL were optimized to 2.84 and $6.68\%$, respectively. CPC produc­tion was improved $50\%$ by feeding of $5\%$ rice oil at day 3rd and 5th day during the shake flask culture of C acremonium M25. The effect of agitation speeds on CPC production in a 2.5-L bio­reactor was also investigated with fed-batch mode. The maximum cell mass (54.5 g/L) was obtained at 600 rpm. However, the maximum CPC production (0.98 g/L) was obtained at 500 rpm. At this condition, the maximum CPC production was improved about $132\%$ compared to the re­sult with batch flask culture.