• Asian-Australasian Journal of Animal Sciences
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• v.32 no.8
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• pp.1186-1194
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• 2019

Objective: The influence of broiler carcass scalding conditions on chicken breast meat quality parameters was investigated. Methods: Two hundred and seventy Cobb broiler chickens from 42 to 48 days old were slaughtered according to the standard industry practice and scalded in five temperature/time combinations-$T_1$, $54^{\circ}C/210s$; $T_2$, $55^{\circ}C/180s$; $T_3$, $56^{\circ}C/150s$; $T_4$, $57^{\circ}C/120s$; $T_5$, $58^{\circ}C/90s$. Results: Scalding temperature increase resulted in higher values of external and ventral lightness and in protein functionality reduction-determined by emulsification capacity and protein denaturation-in chicken breast fillets 24 h post-mortem. Protein secondary structures had conformational changes, with a decrease of the ${\alpha}$-helix and an increase of the ${\beta}$-sheet and ${\beta}$-turn proportions, mainly in $T_1$ and $T_5$ samples, determined by Fourier-transform infrared spectroscopy in an attenuated reflectance mode analysis. The chemical composition, pH, water holding capacity and Warner-Bratzler shear force did not differ among the treatments. In the fatty acid profile, the 18:1n-9 was lower in $T_5$, which suggested that the high scalding-temperature could have caused the lipid oxidation. The values of the polyunsaturated fatty acids (PUFA), such as 22:2, 20:4n-6, and 22:6n-3, were highest in the $T_5$, thus being related to the phospholipid cellular membrane collapse in this experimental condition and subsequent release of these PUFA. Conclusion: Intermediate scalding-parameters avoided the negative changes in the chicken meat quality.

• Journal of the Korea Safety Management & Science
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• v.15 no.3
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• pp.61-69
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• 2013

According to ISO 26262 (the international standard on functional safety for automotive industry), the functional safety should be considered during the whole automotive systems life cycle from the design phase throughout the production phase. In order to satisfy the standard, the automotive and related industry needs to take appropriate actions while carrying out a variety of development activities. This paper presents an approach to coping with the standard. Analyzing the standard indicates that the safety issues of the automotive systems should be handled with a system's view whereas the conventional approach to solving the issues has been practiced with focus on the component's level. The aforementioned system's view implies that the functional safety shall be incorporated in the system design from both the system's life-cycle view and the hierarchical view for the structure. In light of this, the systems engineering framework can be quite appropriate in the functional safety development and thus has been taken in this paper as a problem solving approach. Of various design issues, the analysis and verification of the safety requirements for functional safety is a key study subject of the paper. Note, in particular, that the conventional FMEA (failure mode effects analysis) and FTA (fault tree analysis) methods seem to be partly relying on the insufficient experience and knowledge of the engineers. To improve this, a systematic method is studied here and the result is applied in the design of an ABS braking system as a case study.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.23
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• pp.10397-10400
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• 2015

Stigmalactam, an aristolactam-type alkaloid extracted from Orophea enterocarpa, exerts cytotoxicity against several human and murine cancer cell lines, but the molecular mechanisms remain elusive. The aims of this study were to identify the mode and mechanisms of human cancer cell death induced by stigmalactam employing human hepatocellular carcinoma HepG2 and human invasive breast cancer MDA-MB-231 cells as models, compared to normal murine fibroblasts. It was found that stigmalactam was toxic to HepG2 and MDA-MB-231 cells with $IC_{50}$ levels of $23.0{\pm}2.67{\mu}M$ and $33.2{\pm}4.54{\mu}M$, respectively, using MTT assays. At the same time the $IC_{50}$ level towards murine normal fibroblast NIH3T3 cells was $24.4{\pm}6.75{\mu}M$. Reactive oxygen species (ROS) production was reduced in stigmalactam-treated cells dose dependently after 4 h of incubation, indicating antioxidant activity, measured by using 2',7',-dichlorohydrofluorescein diacetate and flow cytometry. Caspase-3 and caspase-9 activities were increased in a dose response manner, while stigmalactam decreased the mitochondrial transmembrane potential dose-dependently in HepG2 cells, using 3,3'-dihexyloxacarbocyanine iodide and flow cytometry, indicating mitochondrial pathway-mediated apoptosis. In conclusion, stigmalactam from O. enterocarpa was toxic to both HepG2 and MDA-MB-231 cells and induced human cancer HepG2 cells to undergo apoptosis via the intrinsic (mitochondrial) pathway.

• Journal of the Korea Convergence Society
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• v.6 no.6
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• pp.75-80
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• 2015

To train convergence experts ICT infrastructure must be learned IoT use capabilities of things. In this paper, targeted to students that are majoring in computer science, and for the design of the curriculum and the educational model that handles education of operation in general for training IoT-related education for five months to experts of convergence training describe the contents. Course is the basic ability of the process, core competence course, is divided into real-life capability process and on-site training course, was constructed in a total of 10 stages of the process details. Curriculum, it was designed to learn sensing technology, network technology, security, and content production technology. This educational model is utilized for job creation human resource training project of 2015 Ministry of Employment and Labor, it demonstrated the utility to contribute in order to achieve a high completion and the employment rate. Applicable to future university education plans to expand the curriculum, including courses that complement the Java.

• Journal of Microbiology and Biotechnology
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• v.8 no.3
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• pp.203-207
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• 1998

Fed-batch fermentations with different feeding media were carried out in order to increase the productivity of invertase expression using a recombinant Saccharomyces cerevisiae containing plasmid pRB58. Two batch cultures showed the expression of the SUC2 gene at a low concentration of glucose, suggesting that glucose concentration could be used as a control variable in a fed-batch operation mode. In the fed-batch culture by feeding the basal medium, cell mass and specific invertase activity did not increase much as compared with the simple batch culture. A series of fed-batch cultures revealed that the sucrose-supplemented medium increased cell mass whereas the enriched medium did specific invertase activity. To capitalize on the synergism of the sucrose-supplemented medium and the enriched medium, the sucrose-supplemented enriched medium was used as a feeding medium. The fed-batch culture using this medium resulted in a 2.4-fold increase in cell mass and a 1.9-fold enhancement in specific invertase activity compared with those of the batch culture. The increase in cell mass and specific invertase activity led to a marked increase in total invertase activity, 250U/ml, which was 6.3 times higher than that of the batch culture.

• Microbiology and Biotechnology Letters
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• v.46 no.1
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• pp.40-44
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• 2018

An isolate capable of inhibiting the growth of gram-positive bacteria was obtained from the soil of Mushim stream, Cheongju. The isolate was identified as Pseudomonas otitidis PS by 16S rRNA gene sequence analysis. P. otitidis PS produced antibiotics as a secondary metabolite when cultured in 1% soybean meal with 0.5% glucose. The maximum yield was about 0.1%. The antibiotic substance of P. otitidis PS extracted using ethyl acetate displayed a minimum inhibitory concentration of $2{\mu}g/ml$ for Staphylococcus aureus KCTC 1261. The antibiotic substance produced an orange halo on chrome azurol S agar due to siderophore activity. Growth inhibition was decreased when the iron was depleted. Since the antibiotic activity was lost upon the addition of the reducing agent ascorbic acid or during anaerobic culture, it was considered that antibiotic of P. otitidis PS strain exerts its bactericidal effect by the generation of reactive oxygen species.

• Ocean Systems Engineering
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• v.6 no.3
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• pp.265-287
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• 2016

The change of the global performance of a turret-moored FPSO (Floating Production Storage Offloading) with DP (Dynamic Positioning) control is simulated, analyzed, and compared for two different internal turret location cases; bow and midship. Both collinear and non-collinear 100-yr GOM (Gulf of Mexico) storm environments and three cases (mooring-only, with DP position control, with DP position+heading control) are considered. The horizontal trajectory, 6DOF (degree of freedom) motions, fairlead mooring and riser tension, and fuel consumptions are compared. The PID (Proportional-Integral-Derivative) controller based on LQR (linear quadratic regulator) theory and the thrust-allocation algorithm which is based on the penalty optimization theory are implemented in the fully-coupled time-domain hull-mooring-riser-DP simulation program. Both in collinear and non-collinear 100-yr WWC (wind-wave-current) environments, the advantage of mid-ship turret is demonstrated by the significant reduction in heave at the turret location due to the minimal coupling with pitch mode, which is beneficial to mooring and riser design. However, in the non-collinear WWC environment, the mid-turret case exhibits unfavorable weathervaning characteristics, which can be reduced by employing DP position and heading controls as demonstrated in the present case studies. The present study also reveals the plausible cause of the failure of mid-turret Gryphon Alpha FPSO in milder environment than its survival condition.

• Journal of the Korean Society of Industry Convergence
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• v.26 no.1
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• pp.95-103
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• 2023

The domestic remanufacturing industry is concentrated in auto parts, so it is necessary to expand into various industries. In the domestic aluminum industry, the extrusion process accounts for more than 40% of the total, but the old and management of the extrusion equipment is not done properly. In particular, the extruder has a structure in which equipment is not replaced until major parts are damaged or worn, so there are problems such as lower process precision, productivity and production efficiency compared to new equipment, and high maintenance costs. In this study, the old extruder was remanufactured for major high-risk parts through Failure Mode and Effect Analysis(FMEA), and the process level and performance of the extruder were evaluated before and after remanufacturing. Compared to the existing extruder, the standard deviation of the remanufacture extruder was reduced by 93.5%, 57.9%, and 70.0%, respectively, in major process control items such as container temperature, billet temperature, and ram speed, keeping performance constant. In addition, it was possible to produce products with complex shapes that could not be produced before due to problems such as dimensional deviation within tolerances. In this study, remanufacturing guidelines were presented by analyzing the effect of failure modes of the old extruder, and the performance improvement of the extruder was confirmed.

• Microbiology and Biotechnology Letters
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• v.16 no.3
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• pp.187-192
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• 1988

Production of 4-androstene-3,17-dione(AD) from cholesterol by microbial conversion was investigated. To facilitate the solubilization of cholesterol in the fermentation broth, ethanol was used as an organic solvent. Inhibition on cell growth by ethanol was observed to be negligible upto 2% (V/V) concentration. Microbial conversion was successfully carried out with high yield when the cholesterol was added at early logarithmic growth phase with pH control at 7.0. In order to improve the process productivity, bioconversion was conducted at various mode of cholesterol addition ; 0.1% (V/W) of cholesterol was found to be most appropriate for solubilization in ethanol and was added intermittently. When added three time(total 3 g/$\ell$), overall bioconversion yield reached upto 65% while single addition of same amount of cholesterol (3 g/$\ell$) yielded about 40% conversion.

• Journal of IKEEE
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• v.21 no.3
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• pp.252-255
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• 2017

$Mo_xW_{1-x}Si_2$ heaters were fabricated by self-propagating high-temperature synthesis (SHS) process and post sintering process. To validate the reliability of the $Mo_xW_{1-x}Si_2$ heaters, the accelerated life test (ALT) was conducted, and then lifetime to $Mo_xW_{1-x}Si_2$ heaters was estimated by using Minitab programs. Also, the failure analysis of $Mo_xW_{1-x}Si_2$ heaters after ALT was performed through electrical and structural properties. As the results, it was confirmed that the dominant failure mode of $Mo_xW_{1-x}Si_2$ heaters is the crack formation in heaters and the delamination of protective $SiO_2$ layers.