• Steel and Composite Structures
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• 제29권4호
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• pp.451-464
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• 2018

This paper presents some quasi-static tests for 4 mixed columns composed of CFST column and RC column. The seismic performance and failure mode were studied under low-cyclic revised loading. The failure mode was observed under different axial compression ratios. The hysteretic curve and skeleton curve were obtained. The effects of axial compression ratio on yield mechanism, displacement ductility, energy dissipation, stiffness and strength attenuation were analyzed. The results indicate that the failure behavior of CFST-RC mixed column with archaized style is mainly caused by bending failure and accompanied by some shear failure. The axial compression ratio performs a control function on the yielding order of the upper and lower columns. The yielding mechanism has a great influence on the ductility and energy dissipation capacity of specimens. Based on the experiment, finite element analysis was made to further research the seismic performance by ABAQUS software. The variable parameters were stiffness ratio of upper and lower columns, axial compression ratio, yielding strength of steel tube, concrete strength and rebar ratio. The simulation results show that with the increase of stiffness ratio of the upper and lower columns, the bearing capacity and ductility of specimens can correspondingly increase. As the axial compression ratio increases, the ductility of the specimen decreases gradually. The other three parameters both have positive effect on the bearing capacity but have negative effect on the ductility. The results can provide reference for the design and engineering application of mixed column consisted of CFST-RC in Chinese archaized buildings.

• Bulletin of the Korean Chemical Society
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• 제31권2호
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• pp.483-486
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• 2010

• Journal of Microbiology and Biotechnology
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• 제9권3호
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• pp.292-296
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• 1999

The high density mixed mode adsorbent known by the trade name of Mimo-AD was used to purify lysozyme directly from the hen egg white (HEW). The homogenized hen egg white was treated with the adsorbent in a stirred vessel for lysozyme adsorption, and then the adsorbent, easily separated from the HEW by sedimentation, was packed into a column. The remaining HEW and contaminant proteins were removed by washing with pH 11 distilled water in an expanded-bed state, and subsequently the elution was performed with pH 12 distilled water in a packed-bed state. By this simple and rapid adsorption, washing, and elution procedure, lysozyme was purified to＞95％ with an overall recovery yield of 66％. This process offers a great potential for industrial application by allowing the extraction of lysozyme while retaining the commercial value of HEW.

• 한국전산구조공학회논문집
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• 제12권3호
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• pp.363-370
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• 1999

본 연구에서는 철근콘크리트기둥과 철골보로 이루어진 혼합구조 접합부의 해석에 대한 유한 유소법을 이용한 해석 모델 방법을 제시하였다. 혼합구조 접합부에서 콘크리트와 강판이 접하는 접촉면은 두 접촉면 사이를 부착과 마찰의 개념으로 표현할 수 있는 주-종속 접촉 알고리즘(master-slave contact algorithm)을 이용하여 모델링하였다. 그리고, 휨응력의 지배를 받는 강관에는 비적합 모드 요소를 사용하였다. 본 연구에서의 혼합구조 특징은 보에서 기둥으로 힘의 전달을 원활히 하기 위하여 다이아프램이 사용되었고, 이러한 혼합구조 접합부 모델링 방법에 대한 타당성을 알아보기 위하여 3차원 비선형 해석을 행하여 실험결과와 비교한 결과 잘 일치하는 결과를 얻었다.

• Structural Engineering and Mechanics
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• 제68권2호
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• pp.193-201
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• 2018

This paper describes an experimental study on the seismic performance of steel reinforced concrete (SRC) T-shaped columns. The lateral loads were applied along the web of the column with different loading histories, such as monotonic loading, mixed loading of variable amplitude cyclic loading and monotonic loading, constant amplitude cyclic loading and variable amplitude cyclic loading. The failure modes, load-displacement curves, characteristic loads and displacements, ductility, strength and stiffness degradations and energy dissipation capacity of the column were analyzed. The effects of loading history on the seismic performance were focused on. The test results show that the specimens behaved differently in the aspects of the failure mode subject to different loading history, although all the failure modes can be summarized as flexural failure. The hysteretic loops of specimens are plump, and minimum values of the failure drift angles and ductility coefficients are 1/24 and 4.64, respectively, which reflect good seismic performance of SRC T-shaped column. With the increasing numbers of loading cycles, the column reveals lower bearing capacity and ductility. The strength and stiffness of the column with variable amplitude cyclic loading degrades more rapidly than that with constant amplitude cyclic loading, and the total cumulative dissipated energy of the former is less.

• Mass Spectrometry Letters
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• 제9권1호
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• pp.30-36
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• 2018

A quantitation method for free amino acids in human serum was developed using a stepwise-dilution method and a bimodal cation exchange (CEX)/hydrophilic interaction liquid chromatography (HILIC)-tandem mass spectrometry system equipped with an electrospray ionization source (ESI/MS/MS). This method, which was validated using quality control samples, was optimized for enhanced selectivity and sensitivity. Dithiothreitol (DTT) was used as a reducing agent to prevent the oxidation of a serum sample ($50{\mu}L$), which was then subjected to stepwise dilution using 3, 30, and 90 volumes of acetonitrile containing 0.1% formic acid. Chromatographic separation was performed on an Imtakt Intrada Amino Acid column ($50mm{\times}3mm$, $3{\mu}m$) in mixed mode packed with CEX and HILIC ligands embedded in the stationary phase. Underivatized free amino acids were eluted and separated within 10 min. As a result of the validation, the precision and accuracy for the inter- and intraday assays were determined as 2.11-11.51% and 92.82-109.40%, respectively. The lowest limit of quantification (LLOQ) was $0.5-4.0{\mu}g/mL$ and the matrix effect was 80.22-115.93%. The proposed method was successfully applied to the quantitative analysis of free amino acids in human serum.

• KSBB Journal
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• 제11권5호
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• pp.524-528
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• 1996

과립활성탄(GAC)상에 고정된 세균에 의한 4chloro-2-methylphenoxyacetic acid (MCPA)의 분해가 유통 조건하의 컬럼반응조에서 연구되었다. GAC상에 생물막을 형성시키기 위한 접종으로서 M MCPA를 분해하는 세균의 혼합배양이 사용되었다. 접종하기 전에 GAC에 의한 MCPA의 흡착을 조사 하였으며 접종후 GAC상에 형성된 생물막을 주사전자 현미경으로 관찰하였다. MCPA 분해는 생물막이 형성된 GAC를 포함하는 컬럼반응조의 회분 및 연 속운전 조건하에서 조사되었다. 회분배양에서 MC P PA의 완전분해가 이루어졌다. 연속배양상태에서 분적 인 MCPA의 분해가 일어났으나 분해정도는 기질인 MCPA의 결럼반응조내로 유입속도에 의존 었다.

• International Journal of Naval Architecture and Ocean Engineering
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• 제5권4호
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• pp.513-528
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• 2013

A floating Oscillating Water Column (OWC) wave energy converter, a Backward Bent Duct Buoy (BBDB), was simulated using a state-of-the-art, two-dimensional, fully-nonlinear Numerical Wave Tank (NWT) technique. The hydrodynamic performance of the floating OWC device was evaluated in the time domain. The acceleration potential method, with a full-updated kernel matrix calculation associated with a mode decomposition scheme, was implemented to obtain accurate estimates of the hydrodynamic force and displacement of a freely floating BBDB. The developed NWT was based on the potential theory and the boundary element method with constant panels on the boundaries. The mixed Eulerian-Lagrangian (MEL) approach was employed to capture the nonlinear free surfaces inside the chamber that interacted with a pneumatic pressure, induced by the time-varying airflow velocity at the air duct. A special viscous damping was applied to the chamber free surface to represent the viscous energy loss due to the BBDB's shape and motions. The viscous damping coefficient was properly selected using a comparison of the experimental data. The calculated surface elevation, inside and outside the chamber, with a tuned viscous damping correlated reasonably well with the experimental data for various incident wave conditions. The conservation of the total wave energy in the computational domain was confirmed over the entire range of wave frequencies.

• 한국조명전기설비학회:학술대회논문집
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• 한국조명전기설비학회 1996년도 추계학술발표회논문집
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• pp.53-56
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• 1996

In this paper, the pulsed mode operated gas discharge tube is composed with mixed gas of Hg-Ne (10 Torr), in the tube of 15.0 mm outer diameter and has variable color from red to blue with changing frequency high voltage. As increasing frequency in the gas discharge tube, the phenomena, that the electron temperature in the positive column increases and the radiation from atoms of higher upper state energy levels increases, exist The color have the locus from red (0.4972, 0.3128) to blue (0.2736, 0.2619) in CIE chromacity diagram with increasing frequency. The method of changing frequency has been shown to be suitable for the luminous color control.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2009년도 춘계 학술대회 제21권1호
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• pp.453-454
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• 2009

본 연구에서는 중 고층 철근콘크리트 주상복합형 건축물에서 지진하중과 같은 반복하중이 작용할 때 단차형 내부 보-기둥 접합부의 거동 특성을 파악하기 위하여 단차 유무에 따른 내부 보-기둥 접합부 실험체를 대상으로 실험을 실시하여 다음과 같은 결론을 얻었다. 단차가 있는 내부 보-기둥 접합부 실험체는 단차가 없는 표준실험체(FJ-0D)와 비교하여 단차 수준(1.0D, 2.0D)이 증가함에 따라 수평내력과 에너지소산능력이 증가하였고 내부 보-기둥 접합부 영역을 중심으로 균열진전 상황이 상이하였다. 또한 에너지소산능력은 내부 보-기둥 접합부 단차가 높을수록 1.1${\sim}$1.35배 증가하였다.