• Steel and Composite Structures
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• v.29 no.4
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• pp.451-464
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• 2018

This paper presents some quasi-static tests for 4 mixed columns composed of CFST column and RC column. The seismic performance and failure mode were studied under low-cyclic revised loading. The failure mode was observed under different axial compression ratios. The hysteretic curve and skeleton curve were obtained. The effects of axial compression ratio on yield mechanism, displacement ductility, energy dissipation, stiffness and strength attenuation were analyzed. The results indicate that the failure behavior of CFST-RC mixed column with archaized style is mainly caused by bending failure and accompanied by some shear failure. The axial compression ratio performs a control function on the yielding order of the upper and lower columns. The yielding mechanism has a great influence on the ductility and energy dissipation capacity of specimens. Based on the experiment, finite element analysis was made to further research the seismic performance by ABAQUS software. The variable parameters were stiffness ratio of upper and lower columns, axial compression ratio, yielding strength of steel tube, concrete strength and rebar ratio. The simulation results show that with the increase of stiffness ratio of the upper and lower columns, the bearing capacity and ductility of specimens can correspondingly increase. As the axial compression ratio increases, the ductility of the specimen decreases gradually. The other three parameters both have positive effect on the bearing capacity but have negative effect on the ductility. The results can provide reference for the design and engineering application of mixed column consisted of CFST-RC in Chinese archaized buildings.

• Bulletin of the Korean Chemical Society
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• v.31 no.2
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• pp.483-486
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• 2010

• Journal of Microbiology and Biotechnology
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• v.9 no.3
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• pp.292-296
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• 1999

The high density mixed mode adsorbent known by the trade name of Mimo-AD was used to purify lysozyme directly from the hen egg white (HEW). The homogenized hen egg white was treated with the adsorbent in a stirred vessel for lysozyme adsorption, and then the adsorbent, easily separated from the HEW by sedimentation, was packed into a column. The remaining HEW and contaminant proteins were removed by washing with pH 11 distilled water in an expanded-bed state, and subsequently the elution was performed with pH 12 distilled water in a packed-bed state. By this simple and rapid adsorption, washing, and elution procedure, lysozyme was purified to＞95％ with an overall recovery yield of 66％. This process offers a great potential for industrial application by allowing the extraction of lysozyme while retaining the commercial value of HEW.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.12 no.3
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• pp.363-370
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• 1999

This study presented an modeling method for the connections in mixed structure with reinforced concrete columns and steel beam using finite element method. The contacting surfaces between concrete and steel are modeled using master-slave contact algorithm and the incompatible mode elements were used in the steel tube subjected to bending. The characteristics of mixed structure was that diaphragm was used for transferring force from beam to column. The three dimensional nonlinear analysis was performed and the analytical results compared with experimental results in order to prove modeling method.

• Structural Engineering and Mechanics
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• v.68 no.2
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• pp.193-201
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• 2018

This paper describes an experimental study on the seismic performance of steel reinforced concrete (SRC) T-shaped columns. The lateral loads were applied along the web of the column with different loading histories, such as monotonic loading, mixed loading of variable amplitude cyclic loading and monotonic loading, constant amplitude cyclic loading and variable amplitude cyclic loading. The failure modes, load-displacement curves, characteristic loads and displacements, ductility, strength and stiffness degradations and energy dissipation capacity of the column were analyzed. The effects of loading history on the seismic performance were focused on. The test results show that the specimens behaved differently in the aspects of the failure mode subject to different loading history, although all the failure modes can be summarized as flexural failure. The hysteretic loops of specimens are plump, and minimum values of the failure drift angles and ductility coefficients are 1/24 and 4.64, respectively, which reflect good seismic performance of SRC T-shaped column. With the increasing numbers of loading cycles, the column reveals lower bearing capacity and ductility. The strength and stiffness of the column with variable amplitude cyclic loading degrades more rapidly than that with constant amplitude cyclic loading, and the total cumulative dissipated energy of the former is less.

• Mass Spectrometry Letters
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• v.9 no.1
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• pp.30-36
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• 2018

A quantitation method for free amino acids in human serum was developed using a stepwise-dilution method and a bimodal cation exchange (CEX)/hydrophilic interaction liquid chromatography (HILIC)-tandem mass spectrometry system equipped with an electrospray ionization source (ESI/MS/MS). This method, which was validated using quality control samples, was optimized for enhanced selectivity and sensitivity. Dithiothreitol (DTT) was used as a reducing agent to prevent the oxidation of a serum sample ($50{\mu}L$), which was then subjected to stepwise dilution using 3, 30, and 90 volumes of acetonitrile containing 0.1% formic acid. Chromatographic separation was performed on an Imtakt Intrada Amino Acid column ($50mm{\times}3mm$, $3{\mu}m$) in mixed mode packed with CEX and HILIC ligands embedded in the stationary phase. Underivatized free amino acids were eluted and separated within 10 min. As a result of the validation, the precision and accuracy for the inter- and intraday assays were determined as 2.11-11.51% and 92.82-109.40%, respectively. The lowest limit of quantification (LLOQ) was $0.5-4.0{\mu}g/mL$ and the matrix effect was 80.22-115.93%. The proposed method was successfully applied to the quantitative analysis of free amino acids in human serum.

• KSBB Journal
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• v.11 no.5
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• pp.524-528
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• 1996

Bacterial degradation of 4-chloro-2-methylphenoxyacetic acid (MCPA) was studied in column reactors under conditions approximating a fluidized bed system, with granular activated carbon (GAC) as a support matrix. A mixed bacterial culture of MCPA-degrading bacteria was used as an inoculum to develop a biofilm on GAC. Initially, adsorption of MCPA by GAC and blofilm formation on GAC were examined. MCPA degradation was evaluated with a batch and continuous mode of operation of the GAC fixed-film column reactors. In the batch operations, complete degradation of MCPA was achieved during the incubation period. Partial degradation of MCPA occurred in the continuous operations and MCPA degradation was dependent on the feeding rate of MCPA solution.

• International Journal of Naval Architecture and Ocean Engineering
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• v.5 no.4
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• pp.513-528
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• 2013

A floating Oscillating Water Column (OWC) wave energy converter, a Backward Bent Duct Buoy (BBDB), was simulated using a state-of-the-art, two-dimensional, fully-nonlinear Numerical Wave Tank (NWT) technique. The hydrodynamic performance of the floating OWC device was evaluated in the time domain. The acceleration potential method, with a full-updated kernel matrix calculation associated with a mode decomposition scheme, was implemented to obtain accurate estimates of the hydrodynamic force and displacement of a freely floating BBDB. The developed NWT was based on the potential theory and the boundary element method with constant panels on the boundaries. The mixed Eulerian-Lagrangian (MEL) approach was employed to capture the nonlinear free surfaces inside the chamber that interacted with a pneumatic pressure, induced by the time-varying airflow velocity at the air duct. A special viscous damping was applied to the chamber free surface to represent the viscous energy loss due to the BBDB's shape and motions. The viscous damping coefficient was properly selected using a comparison of the experimental data. The calculated surface elevation, inside and outside the chamber, with a tuned viscous damping correlated reasonably well with the experimental data for various incident wave conditions. The conservation of the total wave energy in the computational domain was confirmed over the entire range of wave frequencies.

• Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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• 1996.11a
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• pp.53-56
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• 1996

In this paper, the pulsed mode operated gas discharge tube is composed with mixed gas of Hg-Ne (10 Torr), in the tube of 15.0 mm outer diameter and has variable color from red to blue with changing frequency high voltage. As increasing frequency in the gas discharge tube, the phenomena, that the electron temperature in the positive column increases and the radiation from atoms of higher upper state energy levels increases, exist The color have the locus from red (0.4972, 0.3128) to blue (0.2736, 0.2619) in CIE chromacity diagram with increasing frequency. The method of changing frequency has been shown to be suitable for the luminous color control.

• Proceedings of the Korea Concrete Institute Conference
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• 2009.05a
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• pp.453-454
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• 2009

In this dissertation, experimental program was carried out to study the hysteretic behavior of the reinforced different floor type interior beam-column joint repeated cyclic loads under seismic actions. The test results was as follow. The reinforced interior beam-column joint, designed by the different floor type, was increased energy dissipation capacity and maximum load carrying capacity according to the increase of different floor in comparison to standard specimen. And it was also dissimilar to failure mode adjacent to joint region. energy dissipation capacity of each specimen, designed by the different floor type, was increased 1.1${\sim}$1.35 times in comparison to standard specimen.