• Journal of Periodontal and Implant Science
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• 제40권6호
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• pp.265-270
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• 2010

Purpose: The aim of this study was to investigate the immunomodulatory effects of canine periodontal ligament stem cells on allogenic and xenogenic immune cells in vitro. Methods: Mixed cell cultures consisting of canine stem cells (periodontal ligament stem cells and bone marrow stem cells) and allogenic canine/xenogenic human peripheral blood mononuclear cells (PBMCs) were established following the addition of phytohemagglutinin. The proliferation of PBMCs was evaluated using the MTS assay. The cell division of PBMCs was analyzed using the CFSE assay. The apoptosis of PBMCs was assessed using the trypan blue uptake method. Results: Periodontal ligament stem cells and bone marrow stem cells inhibited the proliferation of allogenic and xenogenic PBMCs. Both periodontal ligament stem cells and bone marrow stem cells suppressed the cell division of PBMCs despite the existence of a mitogen. No significant differences in the percentages of apoptotic PBMCs were found among the groups. Conclusions: Canine periodontal ligament stem cells have an immunomodulatory effect on allogenic and xenogenic PBMCs. This effect is not a product of apoptosis of PBMCs but is caused by the inhibition of cell division of PBMCs.

• Asian-Australasian Journal of Animal Sciences
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• 제31권8호
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• pp.1098-1102
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• 2018

Objective: Temperament can be defined as a type of behavioral tendency that appears in a relatively stable manner in responses to various external stimuli over time. The aim of this study was to estimate genetic parameters for the records of temperament testing that are used to improve the temperament of Jeju crossbred (Jeju${\times}$Thoroughbred) horses. Methods: This study was conducted using 205 horses (101 females and 104 males) produced between 2010 and 2015. The experimental animals were imprinted and tamed according to the Manual for Horse Taming and Evaluation for Therapeutic Riding Horses and evaluated according to the categories for temperament testing (gentleness, patience, aggressiveness, sensitivity, and friendliness) between 15 months and 18 months of age. Each category was scored on a five-point linear scale. Genetic parameters for the test categories were analyzed using a multi-trait mixed model with repeated records. The ASReml program was used to analyze the data. Results: The heritability of gentleness, patience, aggressiveness, sensitivity and friendliness ranged from 0.08 to 0.53. The standard errors of estimated heritability ranged from 0.13 to 0.17. The test categories showed high genetic correlations with each other, ranging from 0.96 to 0.99 and high repeatability, ranging from 0.70 to 0.73. Conclusion: The results of this study showed that the test categories had moderate heritability and high genetic correlations, but additional studies may be necessary to use the results for the improvement programs of the temperament of Jeju crossbred horses.

• 한국식품과학회지
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• 제22권4호
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• pp.421-425
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• 1990

본 실험에서는 균주의 특성에 따른 유산균수 시험방법의 적합 여부를 알아보기 위해 액상요구르트 제품들을 유효기간 중 여러 실험조건으로 비교 검토하였다. 비교한 실험조건은 배지(BCP, Elliker agar), 배양상태(aerobic, anaerobic), 희석수(saline, phosphate buffer), 희석방법 (10배, 100배)이었으며 $37^{\circ}C$에서 72시간 배양하였다. L. acidophilus 균주를 사용한 액상요구르트의 경우, 배지와 희석방법에 따른 차이는 거의 없었고 희석수와 배양상태에서는 약간의 차이가 있었다. L. jugurti 균주와 L. acidophilus +L. casei 혼합균주의 생우 배지 배양상태, 희석수에서 차이가 있었고 희석방법에는 거의 차이가 없었다. L. casei의 경우 배지, 희석방법에서 약간의 차이를 나타했으며 배양상태는 유산균수에 영향이 없었다. L. bulgaricus의 경우는 배지, 배양상태, 희석방법에 따라 차이가 있었고 희석수는 차이가 없었다. 그러므로 요구르트의 유산균수 측정은 균주의 종류에 따라 가장 좋은 실험조건을 선택하여 실시하는 것이 효과적인 시험방법으로 사료된다.

• 한국지역사회생활과학회지
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• 제19권2호
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• pp.297-308
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• 2008

Previous research on elderly care in rural areas has focused on inter-generational support, ignoring the possibility of elderly care among community members in the rural areas of Korea. This study attempts to explore the roles and potentials of community-based elderly care in rural areas where nearby family or formal services are unavailable. For this purpose, data was collected from the elderly in three Korean rural villages using qualitative case study methods. Each village was studied as a separate case study and in-depth interviews with the elderly in each village were conducted. All interviews were tape-recorded and transcribed verbatim for the analysis. The data was analyzed using the Reflective Qualitative Analytic Technique. Results showed that rural elderly in all three villages had lunch, talked, and played together; almost everyday in the winter. However, the nature of care among rural elderly varied depending on the characteristics of the rural community (the traditional rural, the rural mixed with the urban, and the neighboring rural with the urban). Specifically, the use of mutual aids (providing food, repairing housing, and checking personal safety by telephone or visit, etc.) was most consistent at the traditional rural village. Because both the rural village mixed with the urban and the neighboring rural village with urban have better access to many aids and programs from formal institutions, mutual aids among community members decreased compared with the traditional rural village. However, regular group activities such as sports, dancing or debate help to provide pleasure and integrate the community. These results suggest that community relationships as a substitution for social support provide by family or formal services can be utilized in rural areas.

• 융합신호처리학회논문지
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• 제6권2호
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• pp.53-58
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• 2005

영상에서 신호가 급격히 변화하는 지점은 영상의 특징을 분석함에 있어서 가장 중요한 요소이며, 영상의 위치와 모양 등에 대한 다양한 정보를 포함하고 있다. 그러므로 이러한 에지를 검출하기 위한 많은 연구가 이루어져 왔으며, 초기에 사용한 에지 검출 연산자는 인접한 화소들 사이에 대한 관계를 이용하는 것이었다. 그러나 이와 같은 방법들은 잡음이 존재하는 영상에서는 우수한 성능을 나타내지 못하고, 선택적인 에지 검출이 불가능하다. 한편, 최근 신호처리 분야에서 새로운 기법으로 제시된 웨이브렛 변환은 멀티스케일 에지 검출이 가능하며, 영상에서 에지를 포함한 특징들을 분석하는 분야에 널리 응용되고 있다. 따라서 본 논문에서는 라인 폭에 의존하지 않는 2-D 웨이브렛 함수를 사용하여, 혼합된 잡음환경에서 영상에 존재하는 라인-에지 성분을 검출하였다.

• 한국식품영양학회지
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• 제24권4호
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• pp.535-550
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• 2011

The purpose of this study was to investigate changes in the cooking process of $Bibimbab$(cooked rice mixed with various ingredients) appeared in cook books published after Korean modern era, approximately from late 19th century to the present. 7 cook books were chosen to be analyzed. It is found that the ingredients were mixed with the rice before being served in the cook books written in late 19th century until mid 20th century, while the ingredients were separately decorated on top of the rice in the cook books written from mid 20th century until late 20th century. $Gochujang$(Korean chilly paste), which is common spicy seasoning for $Bibimbab$ in the present time, appeared only in $Hangukeumak$(1987) which is written in late 20th century. Prior to $Hangukeumak$(1987), chilly powder or chilly was used for chilly-based spicy seasoning. Cook books written in late 19th century until mid 20th century, ingredients used for $Bibimbab$ had complicated cooking methods such as $Jeonyueo$(assorted pan-fried delicacies), $Nurumi$(fried beef skewer with various vegetables) and $Sanjeok$(grilled beef skewer). From mid 20th century until late 20th century, among the cook books analyzed in this research, only $Hankukyoribaekguasajeon$(1976) suggested $Jeonyueo$ as an ingredient, and in general, the cooking method for preparing beef became simpler. For further studies, firstly, the cooking procedures used for $Bibimbab$ in the prior period to the Korean modern era need to be examined for more information about the changes of cooking style of $Bibimbab$. Secondly, new $Bibimbab$ recipes for modern restaurants could be created by using the recipes used in the historical cook books. Finally, the definitions of culinary terms used in historical cook books need to be clarified.

• IMMUNE NETWORK
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• 제2권3호
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• pp.133-136
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• 2002

Background: The costimulatory molecule 4-1BB, a member of nerve growth factor receptor/tumor necrosis factor (NGFR/TNFR) super family, is involved in cell survival and death. Methods: In this study, female C57BL/6 ($H-2^b$) mice were used as a recipient, and DBA/2 ($H-2^d$) as a donor to assess a mixed lymphocyte reaction (MLR) and CTL response in vitro, and skin graft survival. IL-2, IFN level was measured by ELISA. Results: Mixed lymphocyte reaction (MLR) analysis showed that 4-1BB-deficient responder cells showed enhanced cellular proliferation over littermate controls. In contrast, IL-2 production was diminished only in 4-1BB knockout cultures. The IFN expression, on the other hand, was comparable between the groups. When female C57BL/6 ($H-2^b$) mice were grafted with the trunk skin of DBA/2 ($H-2^d$) mice, the in vivo tissue destruction of 4-1BB-deficient mice was not distinct from the normal littermates. Conclusion: These data suggest that 4-1BB is critical for the induction of alloreactive responses in vitro but 4-1BB alone could not change the course of skin rejection in vivo.

• 대한간호학회지
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• 제45권3호
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• pp.367-377
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• 2015

Purpose: The purpose of this study was to evaluate the effects of essential oil on oxidative stress, immunity, and skin condition in atopic dermatitis (AD) induced mice. Methods: This study was a $3{\times}3$ factorial design. Factors were oil type (Lavender, Thyme, and 2:1 mixture of lavender and thyme oil [blending oil]) and treatment period (0 day, 7 days, and 21 days). The samples were 45 mice with AD and randomly assigned to nine groups of five mice per group. The dependent variables such as superoxide radical, IgE, degranulated mast cells, and epidermal thickness were measured. Data were collected from February to April in 2014. Descriptive statistics, One-way ANOVA, Two-way ANOVA, and Tukey's HSD test were performed using the SPSS WIN 20.0 program. Results: Dependent variables were not statistically significantly different by the three oil types (p >.05). Essential oils such as lavender, thyme, and blending oil were all effective in reducing AD symptoms and especially 2:1 blending oil were most effective. There were statistically significant differences by the three treatment periods in all dependent variables (p <.001). There were statistically significant interactions between oil types and treatment periods in all dependent variables (p <.01). For decreasing superoxide radical, degranulated mast cells, and epidermal thickness, 2:1 mixed oil should be applied for at least 21 days. Otherwise to reduce IgE, 2:1 mixed oil should be used for at least 7 days. Conclusion: These findings provide bases for developing effective interventions for AD patients to manage their AD symptoms.

• 한국콘텐츠학회논문지
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• 제20권4호
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• pp.49-61
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• 2020

최근 공연예술에서 장르 융합형 콜라보레이션과 기술 융합형 콜라보레이션의 물리적인 융합(convergence)은 서로 다른 목표와 이질적인 속성을 전략적으로 융합시킴으로서 대중의 기대수요에 부응하며 새로운 가치의 융합 콘텐츠를 이끌어내는 실험적인 형태로 발전하고 있다. 융합시도 중 발생되는 문제점을 보완하며 공연의 완성도를 높이기 위해서는 각 매체분야 전문가들의 효율적인 협업과정이 연구되고 개선되어야 한다. 본 논문에서는 다매체 융합공연 프로토타입(Prototype) 제작을 통해 시대의 요구에 상응하는 융합공연의 효율적인 협업제작방안을 연구하고자 한다. 공연제작과정을 프리프로덕션(Pre-Production), 프로덕션(Production)으로 분류하여 라이브 연극과 사전제작 영상 애니메이션, 두 매체를 활용한 융합공연의 효율적인 협업제작과정을 작품선정 및 연출방향 설정, 인적구성, 제작일정 계획수립, 시각화과정, 공연연습 및 리허설 등의 과정으로 연구하고자 한다. 융합공연 콘텐츠 기획, 제작방식의 변화, 융합 콘텐츠 간 특수성의 이해를 바탕으로 지속적인 다매체 융합공연을 위한 효율적인 협업제작과정 연구가 진행되어야 융합 콘텐츠 산업 발전 체계화 및 활성화가 가능할 것이다.

• Journal of Radiation Protection and Research
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• 제37권4호
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• pp.213-218
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• 2012

토양 중 우라늄 동위원소의 분석을 위해서 알파분광분석법이 보편적으로 사용되고 있다. 다수의 토양시료를 분석할 경우 시료분해 시간을 단축함으로써 전체 분석시간을 효과적으로 줄일 수 있으며, 이를 위해 본 연구에서는 단시간에 시료를 완전 분해할 수 있는 마이크로파 용해장치를 활용하였다. IAEA-375를 분석 대상 토양으로 하여 용해되지 않은 시료의 양과 분석한 $^{234}U$과 $^{238}U$의 방사능 농도 값을 통해 시료분해 방법을 최적화 하였으며, 0.5 g의 토양시료에 최소 3 ml의 불산을 가했을 때 80분 내에 효과적으로 분해되는 것을 확인하였다. 이는 2~3일의 분해시간이 필요한 통상적인 방법(open vessel 및 closed vessel을 이용하는 방법)에 비해 시간을 단축 할 수 있어 효율적이며, 불산 사용을 최소화 하여 시료용해 시 발생할 수 있는 유해 물질의 발생 및 접촉을 줄일 수 있다는 장점이 있다.