Ha, Seung-Mo;Cho, Woong;Ahn, Yoo-Min;Hwang, Seung-Yong
Transactions of the Korean Society of Mechanical Engineers A
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v.34
no.12
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pp.1785-1791
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2010
This paper describes a separable microfluidic device fabricated with PDMS (polydimethylsiloxane) and glass. The device is used for sample preparation involving cell lysis and the DNA purification process. The cell lysis was performed for 2 min at $80^{\circ}C$ in a serpentine-type microreactor ($20 {\mu}l$) using a Au microheater that was integrated with a thermal microsensor on a glass substrate. The DNA that was mixed with other residual products during the cell lysis process was then filtered through a new filtration system composed of microbeads (diameter: $50 {\mu}m$) and PDMS pillars. Since the entire process (sample loading, cell lysis reaction, DNA purification, and sample extraction) was performed within 5 min in a microchip, we could reduce the sample preparation time in comparison with that for the conventional methods used in biochemistry laboratories. Finally, we verified the performance of the sample preparation chip by conducting PCR (polymerase chain reaction) analysis of the chip product.
In Juam basin, the ratio of non-point pollution source among pollutant loading of basin was significantly high, since the utilization level of land was high. In addition, the most pollutants were not treated and flowed out. In this study, the correlation between non-point source pollutants from the forest area and increasing algae was investigated. 1. Chl-a concentration flowed out to runoff from forest area and stream water was low as $0.1{\sim}20.3{\mu}g/{\ell}$ and $0.1{\sim}9.3{\mu}g/{\ell}$, respectively, and chl-a concentration ($0.1{\sim}28.5{\mu}g/{\ell}$) of branch stream was higher $5{\sim}7$ times than that of runoff from forest area. 2. In correlation between runoff from forest area and Juam lake water, annual chl-a concentration of area front Juam dam was higher twice than forest area. 3. In runoff from forest area within Juam basin, flagellate, green, diatom and blue algae occupied $33.0{\sim}41.7%$, $22.2{\sim}30.8%$, $17.3{\sim}22.5%$ and $13.7{\sim}17.6%$, respectively. 4. In runoff from forest area, both green and diatom algae were maintained constantly irrespectively of season, and flagellate algae dominated since August. 5. In characteristics by forest tree types, four types algae were inhabited in mixed forest, and flagellate algae were higher in conifer and broadleaf forest than in other area. And green algae in herbaceous forest were higher than other area.
Objectives: This study was conducted to investigate the removal of high odor concentration from swine wastewater treatment facility by full scale biofilter using liquid with deodorant mixed with earthworm cast and distillery sludge. Methods: The supply of the culture liquid to the microorganism on the media in the biofilter increases the activity and growth of biomass. The experimental equipment was biofilter tower with treatment capacity of 90 m 3/min. The experimental conditions included gas flow of $60m^3/min$, retention time of 20 sec, and gas/liquid ratio of 67. Results: With changing season from winter to summer, the inlet odor concentration of ammonia increased from 2.5 ppm to 29 ppm, and of hydrogen sulfide from 21 ppm to 91 ppm, respectively. The odor treatment system with biofilter using the culture liquid was stable when the high loading rate increased and showed excellent removal grade with an average of 96.7% for ammonia, and an average of 93.7% for hydrogen sulfide. The pH and SCOD in the recirculating culture liquid near the bottom of the biofilter tower decreased with operation time, but its influence on the odor removal rate was negligible, because the organic matter (SCOD) was replaced by some culture liquid supplied 2-4 times per day. Conclusions: The biofilter using culture liquid could successfully remove high odor concentration which was generated from swine wastewater treatment facility.
A laboratory experiment was conducted to investigate nitrogen removal from plating wastewater by a soil reactor. A combination of soil, waste oyster shell and activated sludge were used as a loading media in a soil reactor. The addition of 20% waste oyster shell and activated sludge to the soil accelerated nitrification (88.6% ${NH_4}^{+}-N$ removal efficiency) and denitrification (84.3% ${NO_3}^{-}-N$ removal) in the soil reactor, respectively. In continuous removal, the influent ${NH_4}^{+}-N$ was mostly converted to nitrate nitrogen in the nitrification soil reactor and only a small amount of ${NH_4}^{+}-N$ was found in the effluent. When methanol was added as a carbon source to the denitrification soil reactor, the average removal efficiency of ${NO_3}^{-}-N$ significantly increased. The ${NO_3}^{-}-N$ removal by methanol addition in the denitrification soil reactor was mainly due to denitrification. The phosphorus was removed by the waste oyster shell media in the nitrification soil reactor. Moreover, the phosphorus removal in the denitrification soil reactor was achieved by synthesis of bacteria and the denitrification under anaerobic conditions. The approximate number of nitrifiers and denitrifiers was $3.3{\times}10^5\;MPN/g$ soil at a depth of $1{\sim}10\;cm$ and $3.3{\times}10^6\;MPN/g$ soil at a depth of $10{\sim}20\;cm$, respectively, in the soil reactor mixed with a waste oyster shell media and activated sludge.
For the process control of silicon nitride gelcasting, the composition effect of acryamide system on the viscosity of slip and mechanical property of gelcast green body were investigated. The slip was prepared by ball milling of silicon nitride suspension prepared with acrylamide monomer and polyelectrolyte dispersant after premixing them by attritor. The slip mixed with initiator was vacuum deaired and cast into molds, and then polymerized. The consolidated green body was obtained by drying the gelated slip. The viscosity measument and the diametral compression test was done to evaluate the rheological behaviro of slip and mechanical property of gelcast body, respectively. Experimental results showed that the high solid loading of silicon nitride slip was obtained up to 46 vol% with a low viscosity. The mechanical property of gelcast body mainly increased with increasing the concentration of monomer. The gelcast body was machinable above the ∼3 MPa of tensile strength. The relative density of pressured-sintered body was 98.5% at 1760$^{\circ}C$, 3 h.
International Journal of Concrete Structures and Materials
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v.9
no.4
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pp.475-486
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2015
Mode II fracture toughness ($K_{IIc}$) of fiber reinforced concrete (FRC) has been widely investigated under various patterns of test specimen geometries. Most of these studies were focused on single type fiber reinforced concrete. There is a lack in such studies for hybrid fiber reinforced concrete. In the current study, an experimental investigation of evaluating mode II fracture toughness ($K_{IIc}$) of hybrid fiber embedded in high strength concrete matrix has been reported. Three different types of fibers; namely steel (S), glass (G), and polypropylene (PP) fibers were mixed together in four hybridization patterns (S/G), (S/PP), (G/PP), (S/G/PP) with constant cumulative volume fraction ($V_f$) of 1.5 %. The concrete matrix properties were kept the same for all hybrid FRC patterns. In an attempt to estimate a fairly accepted value of fracture toughness $K_{IIc}$, four testing geometries and loading types are employed in this investigation. Three different ratios of notch depth to specimen width (a/w) 0.3, 0.4, and 0.5 were implemented in this study. Mode II fracture toughness of concrete $K_{IIc}$ was found to decrease with the increment of a/w ratio for all concretes and test geometries. Mode II fracture toughness $K_{IIc}$ was sensitive to the hybridization patterns of fiber. The (S/PP) hybridization pattern showed higher values than all other patterns, while the (S/G/PP) showed insignificant enhancement on mode II fracture toughness ($K_{IIc}$). The four point shear test set up reflected the lowest values of mode II fracture toughness $K_{IIc}$ of concrete. The non damage defect concept proved that, double edge notch prism test setup is the most reliable test to measure pure mode II of concrete.
In this study, we describe the conventional hot pressing (CHP) of layered $Al-B_4C$ composites and their characterization. The matrix alloy Al-5 wt.%Cu was prepared from elemental powder mixtures. The metal and B4C powders were mixed to produce either $Al-Cu-10vol.%B_4C$ or $Al-Cu-30vol.%B_4C$ combinations. Then, these powder mixtures were stacked as layers in the hot pressing die to form a two-layered composite. Hot pressing was carried out under nitrogen atmosphere to produce $30\times40\times5mm$ specimens. Microstructural features and age hardening characteristics of composites were determined by specimens cut longitudinally. The flexural strength of both layered composites and their monolithic counterparts were investigated via three point bending tests. In the case of layered specimens of both $10vol.%B_4C$ and $30vol.%B_4C$ containing layers were loaded for three-point test. The results show that a homogeneous distribution of $B_4C$ particles in the matrix alloy which is free of pores, can be obtained by CHP method. The ageing behavior of the composites was found to be influenced by the reinforced materials, i.e. higher hardness values were reached in 8 hrs for the composites than that for the matrix alloy. Flexural strength test showed that two-layered composites exhibited improved damage tolerance depending on layer arrangement. Microstructural investigation of the fracture surfaces of the bending specimens was performed by means of scanning electron microscope (SEM). While layer with lower reinforcement content exhibited large plastic deformation under loading, the other with higher reinforcement content exhibited less plastic deformation.
Laboratory experiments were carried out to investigate the performance of anaerobic sequencing batch reactor(ASBR) for digestion of a municipal sludge. Each cycle of the ASBR comprised feeding, two-or three-day reaction, one-day thickening, and withdrawal. The reactors were operated at an HRT of 10days and 5days with an equivalent organic loading rate of 0.8-1.54 gVS/l/d, 1.81-3.56 gVS/l/d at 35$\circ$C, respectively. Solids accumulation was remarkable in the ASBR during start-up period, and directly affected by settleable solids in the feed sludge. Floatation thickening occured in the ASBRs, and Solids profiles at the end of thickening step dramatically changed at solid-liquid interface. Slight difference in solids concentrations was observed within thickened sludge bed. Efficiencies through floatation thickening were comparable to that of additional thickening of the completely mixed control reactor. Average solids concentrations in the ASBRs were 2.2-2.6 times higher than that in the control throughout the total operation period. The dehydrogenase activity had a strong correlation with the solids concentration. Organics removals based on clarified effluent of the ASBRs were consistently above 86%. Remarkable increase in equivalent gas production of 27-52% was observed at the ASBRs compared with the control though the control and ASBRs showed similiar effluent quality. Thus, digestion of a municipal sludge was possible using the ASBR in spite of high concentration of solids in the sludge.
Special track systems used to divert a train to other directions or other tracks are generally called 'railway turnout'. A traditional turnout system consists of steel rails, switches, crossings, steel plates, fasteners, screw spikes, timber bearers, ballast and formation. The wheel rail contact over the crossing transfer zone has a dip-like shape and can often cause detrimental impact loads on the railway track and its components. The large impact also emits disturbing noises (either impact or ground-borne noise) to railway neighbors. In a brown-field railway track where an existing aged infrastructure requires renewal or maintenance, some physical constraints and construction complexities may dominate the choice of track forms or certain components. With the difficulty to seek for high-quality timbers with dimensional stability, a methodology to replace aged timber bearers in harsh dynamic environments is to adopt an alternative material that could mimic responses and characteristics of timber in both static and dynamic loading conditions. A critical review has suggested an application of an alternative material called fibre-reinforced foamed urethane (FFU). The full-scale capacity design makes use of its comparable engineering characteristics to timber, high-impact attenuation, high damping property, and a longer service life. A field trial to investigate in-situ behaviours of a turnout grillage system using an alternative material, 'fibre-reinforced foamed urethane (FFU)' bearers, has been carried out at a complex turnout junction under heavy mixed traffics at Hornsby, New South Wales, Australia. The turnout junction was renewed using the FFU bearers altogether with new special track components. Influences of the FFU bearers on track geometry (recorded by track inspection vehicle 'AK Car'), track settlement (based on survey data), track dynamics, and acoustic characteristics have been measured. Operational train pass-by measurements have been analysed to evaluate the effectiveness of the replacement methodology. Comparative studies show that the use of FFU bearers generates higher rail and sleeper accelerations but the damping capacity of the FFU help suppress vibration transferring onto other track components. The survey data analysis suggests a small vertical settlement and negligible lateral movement of the turnout system. The static and dynamic behaviours of FFU bearers appear to equate that of natural timber but its service life is superior.
Kim, Mi-Ru;Lee, Seung-Jun;Jeong, Jae-Ho;Lee, Deug-Woo
Journal of the Korean Society of Manufacturing Process Engineers
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v.17
no.4
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pp.39-46
/
2018
Two surfaces that have relative motion show different characteristics according to surface roughness or surface patterns in all lubrication areas. For two rough surfaces with mixed lubrication, this paper proposes a new approach that includes the contact characteristics of the surfaces and a probabilistic method for a numerical analysis of lubrication. As the contact area of the two surfaces changes according to the loading conditions, asperity contact is very important. An average flow model developed by Patir-Cheng is central to the study of lubrication for rough surfaces. This average flow model also refers to a multi-asperity contact model for deriving a modified Reynolds equation and calculating the lubricant characteristics of a bearing surface with random roughness during fluid flow. Based on the average flow model, this paper carried out a numerical analysis of lubrication using a contact model by considering a load change made by the actual contact of asperities between two surfaces. Lubrication properties show different characteristics according to the surface patterns. This study modeled various geometric surface patterns and calculated the characteristics of lubrication.
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