• Title/Summary/Keyword: Mixed Extracts

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Cytotoxicity of Ethanol Extract of Raphanuse Sativus on a Human Lung Cancer Cell Line (인체 폐암 세포주에 대한 무의 에탄올 추출물의 세포독성)

  • 임효빈;이건순;채희정
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.2
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    • pp.287-290
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    • 2004
  • This study was desinged to investigate the cytotoxic effect of ethanonl extract of radish, Raphanuse sativus on human lung cancer cell lines (A-549) using MTT(3-〔4,5-dimethylthiazol-2-yl〕-2-yl)-2,5-diphenyltetrazolium bromide) assay. Radish trunk and radish root were extracted by a mixed solvent of water and ethanol (5 : 5, v/v) after drying, The extracts were used for anticancer activity assay. $IC_{50}$/ (50% inhibitory concentration) of radish trunk and radish root were 0.015% and 0.03%, respectively. Radish trunk extract showed higher anticancer activity than radish root extract at the same concentration of 0.01%.

Anti-inflammatory effects of puffed turmeric extract with oriental herb extract in acute colitis mice

  • Kim, Hyunsung;Choi, Yohan;Yu, Seungmin;Go, Gwang-Woong;Baik, Mooyeol;Kim, Wooki
    • Korean Journal of Food Science and Technology
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    • v.52 no.5
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    • pp.482-486
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    • 2020
  • Cases of inflammatory bowel diseases including ulcerative colitis are increasing in Korea and development of non-steroidal anti-inflammatory drugs (NSAIDs) are widely investigated. Natural products with anti-inflammatory properties are rising as safe candidates for NSAIDs. The extract of turmeric or puffed turmeric mixed with herbal extract (goji berry, liquorice, lycium root, and dong quai) was treated to acute colitis mice by oral gavage. The symptoms of colitis, i.e., body weight loss, fecal score, and shortened colon length, were significantly attenuated by puffed turmeric extract with the herbal extract. Non-puffed turmeric extract with herbal extract, however, exhibited a very marginal recovery. Tissue culture supernatant of colons further revealed that both puffed turmeric and non-puffed turmeric extracts with herbal extract suppressed pro-inflammatory cytokine production at a comparable level. These results indicate that puffing is a simple and promising process of turmeric for enhancement of anti-inflammatory properties.

Effects of POLIGONI MULTIFORI RADIX on Imunosuppretion Induced by Methotrexate in Rat (하수오(何首烏)가 methotrexate로 유도된 흰쥐의 면역기능저하(免疫機能低下)에 미치는 영향(影響))

  • Kim Il-Young;Lee Sang-Jae;Kim Kwang-Ho
    • Journal of Society of Preventive Korean Medicine
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    • v.4 no.2
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    • pp.152-169
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    • 2000
  • To examine the effects of Polygonum multiflorum on white rats with deteriorated immunity caused by methotrexate, first of all, methotrexate was fed to the rats once a day for 4 days. After the immune response of the rats are dereriorated, dried extracts of Polygonum multiflorum mixed in water was fed to the white rats once a day for 144days. The next conclusion was made by examining the rates of B-cells and T-cells of the peripheral blood and the changes in rates of CD4+ T-cells and CD8+ T-cell of the blood sampled from the spleen and peripheral region . Polygonum multiflorum has an effect of increasing immune responses on white rats with deteriorated immunity caused by MTX. Especially the count of CD4+ T-cells of the peripheral blood and the count of CD4+ T-cell of the spleen proved the significant effect o( increasing immune responses statistically . Verification of the effects of the Polygonum multiflorum should be made through comparitive .studies using various immune response indexes. Also additional studies for a modern and practicible application of Polygonum multiflorum seems to be needed.

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Separation of dissolved gases from water using synthesized gases based on exhalation characteristics

  • Heo, Pil Woo;Park, In Sub
    • Journal of Advanced Marine Engineering and Technology
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    • v.38 no.10
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    • pp.1347-1353
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    • 2014
  • It's possible for a human to breathe under water, if dissolved oxygen is effectively used. Fish can stay under water using the gill which extracts dissolved oxygen from water. Water includes small amounts of oxygen, so a human needs larger amounts of water to acquire oxygen enough for underwater breathing. The exhalation gas from a human is another method to get higher amounts of oxygen under water. It mainly composes of oxygen, nitrogen and carbon dioxide. So, if only carbon dioxide is decreased, the exhalation gas has good characteristics for breathing of a human under water. In this paper, composition of the exhalation gas from a human was analyzed using GC. Based on these results, the synthesized gas was prepared and mixed into water which was used for experimental devices to analyze separation characteristics of dissolved gases from water. Experimental devices included a water pump, a hollow fiber membrane module and a vacuum pump. The effects of pressure and water flow on separation characteristics of synthesized gas were investigated. The compositions of gases separated from water using synthesized gas were investigated using GC. These results expect to be applied to the development of underwater breathing technology for a human.

Extractions of Chlorophyll from Spinach and Mate Powders and Their Dyeability on Fabrics (시금치와 마테 분말을 이용한 클로로필 추출과 직물 염색)

  • Yoo, Hye Ja;Ahn, Cheunsoon;Narantuya, Lkhagva
    • Journal of the Korean Society of Clothing and Textiles
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    • v.37 no.3
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    • pp.413-423
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    • 2013
  • Chlorophyll is an abundant pigment found in all green plants, algae, and cyanobacteria. This study uses methanol, acetone and water to extract spinach and mate powders in order to examine the possibility of dyeing animal fibers with chlorophyll without chemical alteration. It was shown that methanol extracts of spinach and mate powders can be effectively used to dye wool and silk fabrics if the extract is mixed with water by methanol:water 65:35 v/v. Compared to methanol extract, the acetone extract showed lower chlorophyll yield and lower dye uptake. Water was not an appropriate solvent for chlorophyll extraction and dyeing. Spinach powder showed a higher dye uptake than mate powder due to the higher chlorophyll content than mate powder. It is possible that the chlorophyll dyeing of wool and silk fabrics is due to the hydrogen bonding between the hydroxy amino acids in fiber and the carbonyl groups of chlorophyll. These carbonyl groups are on the heterocyclic ring and the methyl and ethyl side chains of chlorophyll.

A Kurtosis-based Algorithm for Blind Sources Separation Using the Cayley Transformation And Its Application to Multi-channel Electrogastrograms

  • Ohata, Masashi;Matsumoto, Takahiro;Shigematsu, Akio;Matsuoka, Kiyotoshi
    • 제어로봇시스템학회:학술대회논문집
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    • 2000.10a
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    • pp.471-471
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    • 2000
  • This paper presents a new kurtosis-based algorithm for blind separation of convolutively mixed source signals. The algorithm whitens the signals not only spatially but also temporally beforehand. A separator is built for the whitened signals and it exists in the set of para-unitary matrices. Since the set forms a curved manifold, it is hard to treat its elements. In order to avoid the difficulty, this paper introduces the Cayley transformation for the para-unitary matrices. The transformed matrix is referred to as para-skew-Hermitian matrix and the set of such matrices forms a linear space. In the set of all para-skew-Hermitian matrices, the kurtosis-based algorithm obtains a desired separator. This paper also shows the algorithm's application to electrogastrogram datum which are observed by 4 electrodes on subjects' abdomen around their stomachs. An electrogastrogram contains signals from a stomach and other organs. This paper obtains independent components by the algorithm and then extracts the signal corresponding to the stomach from the data.

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A Study on the Characteristics of a series of Autoencoder for Recognizing Numbers used in CAPTCHA (CAPTCHA에 사용되는 숫자데이터를 자동으로 판독하기 위한 Autoencoder 모델들의 특성 연구)

  • Jeon, Jae-seung;Moon, Jong-sub
    • Journal of Internet Computing and Services
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    • v.18 no.6
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    • pp.25-34
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    • 2017
  • Autoencoder is a type of deep learning method where input layer and output layer are the same, and effectively extracts and restores characteristics of input vector using constraints of hidden layer. In this paper, we propose methods of Autoencoders to remove a natural background image which is a noise to the CAPTCHA and recover only a numerical images by applying various autoencoder models to a region where one number of CAPTCHA images and a natural background are mixed. The suitability of the reconstructed image is verified by using the softmax function with the output of the autoencoder as an input. And also, we compared the proposed methods with the other method and showed that our methods are superior than others.

A Study for Acoustic Cues of Pyoung-An Do Dialect Using LPC (LPC를 이용한 평안방언의 음향지표에 관한 연구)

  • Song, Chul-Gyu;Lee, Myoung-Ho;Kim, Young-Bae
    • Journal of Biomedical Engineering Research
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    • v.13 no.3
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    • pp.195-200
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    • 1992
  • This paper deal with the acoustic cues of Pyoung-An Do dialect using linear prediction. Also, this paper descrbes a statistical comparison between standard tone speech data and Pyoung-An Do dia lects. The analysis done mainly focused on the distribution of formants and pitch periods accord to ac- cents variation. For the purpose of objective comparison, the experiments are performed by extracts for- mant LPC spectrum and pithch periods from average magnitude difference function waveforms. Summing up the results, In disyllable words (VCV pattern) , prepositioned vowels have longer phona lion time than postpositioned vowels and the intrin, iii phonation time is whore longer in the low vowels than in the high ones. The africative consonants show the mixed characteristics of the plosive and frlc ative consonants. The remarkable acoustic cues are the low frequency noise-like waves just before the 1st formants in the plosive consonants, the high frequency noise-like waves in the fricative consonants, and phonation time is not affected by the kinds of prepositioned or postpositioned vowels.

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Chemoprotective Effect of Methanol Extract of Hedera rhombea Loaves on the Reversal of Cytochrome P-450 Activities Induced by Carbon Tetrachloride (사염화탄소로 유도된 Cytochrome P-450 활성도의 전환으로 본 Hedera rhombea 잎의 메탄올 추출물의 간독성 감소작용)

  • 홍영숙;김형래;배영숙;박상신
    • Biomolecules & Therapeutics
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    • v.3 no.4
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    • pp.245-250
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    • 1995
  • The carbon tetrachloride($CCl_4$) has been demonstrated to have a hepatotoxic effect in human or many other species. To investigate the enzyme induction of mixed function oxygenases in liver of male Sprague-Dawley rats a single 0.1, 0.5 mι/kg dose of carbon tetrachloride were given. At 24 hr after a single dose of 0.1 mι CC1$_4$/kg weight, methanol extract of Hedera rhombea leaves was administered with 100, 500 mg/kg weight. Assays of 7-ethoxyresorufin-Ο-deethylation(EROD),7-benzyloxyresorufin-Ο-deathylation(BROD),4-nitro-phenol-UDP-glucuronosyltransferase(UDPGT), Western blot and RNA slot blot were used as representatives of the activities of cytochrome P-450 enzymes. The change of the activity of CYP1A1 form measured by EROD assay and Western analysis using 1-7-1 monoclonal antibody was not observed. The activity CYP2B1 form by BROD assay and using 2-66-3 monoclonal antibody was remarkably increased. Elevated level of CYP2B1 mRNA was shown by slot hybridization with 2B1-specific probe. Administration of methanol extract of Hedera rhombea leaves reversed the enzyme activity and the level of mRNA, which suggest the chemoprotective effect of methanol extracts of Hedera rhombea leaves to carbon tetrachloride hepatotoxlcity.

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