• Biomolecules & Therapeutics
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• v.2 no.3
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• pp.286-291
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• 1994

The mutagenicity of DA-3030(rhG-CSF)was studied by reverse mutation test, chromosome aberration test and micronucleus test. The reverse mutatuon test in bacteria was performed using salmonella typhimurium strain TA100, TA98, TA1535 and TA1537 with rhG-CSF in any of the concentrations(150, 75, 37.5, 18.75, 9.375 and 4,6875 $\mu\textrm{g}$/plate), no increase in the number of revertant colonies in each strain was observed, irrespective of treatment with the metabolic activation system(S-9 mix) The chromosome aberration test was carried out using CHL cells, cell line from chinese hamster lung. With 4 doses(75, 37.5, 18.75 and 9.375 $\mu\textrm{g}$/ml) of rhG-/CSF the cells were treated for 24 or 48 hours in the direct method or for 6 hours followed by 18 hour-expression time in the metabolic activation method. Results of the study showed, by the direct method or metabolic activation method, no trend toward increase in the number of aberrant metaphase. The micronucleus test was carried out using ICR mice at the age of 8 weeks. Three doses(862.5, 1725 and 3450 $\mu\textrm{g}$/kg) of DA-3030 were admintstered intraperitoneally with single shot and bone marrow cells were sampled at 24 hours after administration. Neither the number of polychromatic erythrocytes with micronuclei nor the ratio of normochromatic erythrocytes to polychromatic erythrocytes increased singinficantly in each dose, compared with a vehicle control. These results indicate that rhG-CSF has not mutagenic potential under the condiions.

• Safety and Health at Work
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• v.2 no.1
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• pp.17-25
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• 2011

Objectives: In this study, the in vitro mammalian chromosomal aberration (CA) assay was conducted to gain additional information concerning the hazards associated with the use of cyclopentane and ammonium nitrate. While these two chemicals had already been tested by many methods, they had not been studied in the CA test. Methods: The assay was performed using the ovarian infantile cell (CHO-K1 cell), by the direct method (-S9) and by the metabolic activated method (+S9 mix). Results: Using the direct method, the 7 dosages in a 48 hour treatment group did not show that the frequency of CA is proportion to the dosage addition. The frequency of CA is not proportion to the dosage addition for a 6 hour treatment using the metabolic activated method. Conclusion: From these findings, it was decided that the 2 chemicals do not induce chromosomal aberrations under the tested conditions.

• Journal of the Korean Geosynthetics Society
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• v.14 no.1
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• pp.33-41
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• 2015

In this study, laboratory tests (flow, unit weight, unconfined compressive strength and bleeding tests) were performed to evaluate engineering properties of treated soil to improve pipe mixing method. As result, flow and bleeding properties are proportional to the water contents. The unit weight and unconfined compressive strength is decreased as the water contents increased. Comparing equipments contact pressure with bearing capacity of the mixed soil for dozer, backhoe and belt conveyor are immediately appliable. The applicable water content range is estimated from 80% to 200% by pipe mixing method using cement. Also, The design chart is developed from the results.

• The korean journal of orthodontics
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• v.22 no.1
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• pp.123-131
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• 1992

This study was done to evaluate the changes of enamel surface by interproximal stripping and recovery of it by polishing. The number of 34 1st premolars which had extracted for orthodontic treatment were selected as samples. Interproximal stripping was performed by hand with metal strip and strip placer (Dentaurum Co., Germany) and low speed handpiece with diamond disk (Superdiaflex, Germany). Polishing was performed by hand with plastic strip (3M Col) and low speed handpiece with whip-mix, DCPA (Dicalcium Phosphate, Anhydrous, $CaHPO_4$) powder and Sof-lex (3M Co. U.S.A.) polishing kit. Each groups were examined under the scanning electron microscope (JEOL Co., JSM-840A, Japan) and the following results were obtained: 1. The stripped group performed by metal strip and diamond disk altogether showed deep furrow on the enamel surface as wide as about $10{{\mu}m}$. 2. There could be seen more irregular scratched line in the group stripped by metal strip than that by diamond disk. 3. The polished group performed by plastic strip and DCPA powder showed slight smoothening of the edge of stripped furrow on the enamel surface without relation to the stripping method. 4. The polished group performed by Sof-lex progressive polishing kit could not avoid the formation of the furrows on the enamel surface according to the particle size without relation to the stripping method. 5. The polished group performed by the superfine polishing wheel, the final stage of Sof-lex polishing method showed shallow scratched line as wide as within about $2{{\mu}m}$ on the enamel surface without relation to the stripping method. 6. The interproximal stripped enamel surface could not recover its original surface texture by any kind of polishing methods.

• International Journal of Highway Engineering
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• v.7 no.3 s.25
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• pp.63-69
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• 2005

For many years, the compressive strength of concrete has been regarded as an important index in determining concrete pavement quality. The compressive strength of the sample cores from the field has been used as quality index of concrete pavement. However, this process is time consuming and requires a lot of labor. Recently, the M-E Design Methodology in the pavement design based on the elastic modulus has been adopted. Therefore, several NDT methodologies have been adopted for QA/QC in the field and for the pavement design. Among various NDT methods, the wave propagation method can be used to measure the elastic modulus of concrete because the wave velocity is directly related to the elastic modulus. Therefore, in this study the wave propagation method was used for estimating the concrete modulus. The relationship between the compressive strength measured in he laboratory and the elastic modulus measured by the wave propagation method was analyzed, and the compressive strength was estimated from the elastic modulus for various mix types. The results showed that the relationship between the elastic modulus and the compressive strength was observed and the relationship varied depending on the aggregate types.

• Journal of the Korean Chemical Society
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• v.46 no.5
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• pp.419-437
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• 2002

An analytical method for the spectrometric simultaneous determination of the individual ions in the mix-tures of $Fe^{2+}$ and $Fe^{2+}$ ions utilizing a technique of flow injection analysis has been developed. The method was based on the oxidation reaction between $Fe^{2+}$ ion and $H_2O_2$ in an acidic medium and the subsequent formation of a red Fe$(SCN)^{3-x}_x$ ion by the complexation reaction between $Fe^{2+}$ ion and $SCN^-$ ion. Unlike the conventional methods which require separate processes for the pre-treatment of the sample solution, the current method uses the same FIA system for the pre-treatment and the analysis of the sample. The detection limit for the determination of $Fe^{2+}$ ion was found to be 6.00${\times}10^{-7}$M.

• Journal of the Korea Institute of Building Construction
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• v.14 no.5
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• pp.397-405
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• 2014

There are many literatures reporting that the service life of re-bars in concrete structures is reduced in the oceanic environment due to chloride attack. To solve this problem, this study used geo-polymer as a mix material for concrete to increase its resistance to salt damage, and the external voltage method, one of the electric methods, is was applied to evaluate the likelihood of re-bars in the oceanic structure being exposed to the extreme salt environment. The items evaluated include the natural potential of re-bars and the corrosion rate. The results of the tests showed that in all of the salt environmental conditions (submerged zone, tidal zone, and crack), the tested materials were remarkably effective compared with ordinary concrete. The corrosion protective property was found not only in the evaluation of the natural potential but also in the evaluation of the corrosion rate, suggesting that the external voltage method can be used stably for geo-polymer RC structures in an extreme salt environment.

• Korean Journal of Veterinary Research
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• v.10 no.1
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• pp.25-31
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• 1970

The techniques which have been used for the fecal examination of ruminant infected with the pancreatic flukes, Eurytrema Pancreaticum, were reviewer in their efficiency to detect the ova. One of modified fecal examination: H.F.E. (hydrochloric acid-formalin-ether) sedimentation method was devised in this study. Efficiency in the detecting ability of the fluke eggs with H.F.E. sedimentation method was determined by a series of repeat tests. Among 20 head of cattle known to harbor 1-5 adult worms of the pancreatic fluke, 75% of the infected cattle were detected, and among 60 head of cattle known to harbor more than 6 adult worms, 95% of the infected cattle were detected with H.F.E. sedimentation method. The procedures of the H.F.E. sedimentation method are as follows; 1) Take the sample 5-10 gm., emulsify throughly with 20 ml. of 50% hydrochloric acid in a cup. 2) Strain this mixture through one or two layers of wet surgical gauze into 15ml. centrifuge tube. 3) Washing the cup with 5ml. of 50% hydrochloric acid and strain again. 4) Centrifuge at 2,300 rpm. for 2 minutes. 5) Pour off the supernatant fluid. 6) After the sediment mixed with 10% formalin, stand for 5 minutes. 7) Add 2-3ml. of ether, shake vigorously up and down, after the top of the tube covered with thumb. 8) Centrifuge at 2,300 rpm. for 2 minutes. 9) Loosen the fecal plug in the tube by ringing with an applicator stick. 10) Quickly, but carefully, pour of all, but the bottom layer of sediment. 11) Thoroughly mix the sediment, pour on a slide (or pick up it with a pipett), mount with a cover glass. 12) Examine carefully.

• International Journal of Highway Engineering
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• v.12 no.4
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• pp.203-209
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• 2010

The base asphalt mixtures that used the waste recycle asphalt correcting from the four different overlay construction sites in Seoul city were made using the foamed asphalt method. The sample mixtures were made in different ratio of the recycle asphalt and new asphalt material and the performance of the mixtures of the different ratio was investigated in the laboratory. The laboratory tests includes the Marshall test, the indirect tensile test, the resilient modulus test, the creep test and the wheel tracking test. The test of the recycle foamed asphalt mixtures(RFA) were compared with the those of the recycle hot mix asphalt(RHA) mixtures. The performance of the RFA is comparable to that of the RHA. On the other hand, the indirect tensile strength of the RFA in dry condition is lower than that of RHA and the indirect tensile strength of the RFA in wet condition is much lower than that of the RHA.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.24 no.5
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• pp.541-548
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• 2013

This paper presents a vector network analysis system for 2-port scattering parameters of microwave devices using some basic microwave instruments/devices such as signal generators, vector voltmeter, directional couplers and frequency mixers. The analytical model and implementation method for scattering parameter measurements - which can replace the vector network analyzers - are presented. The performance of the implemented system is evaluated through 1- and 2-port scattering parameter measurements, respectively. The vector volt signals which determine the scattering parameters are detected in two distinct methods depending on the frequency band of interests; a direct-detection method with a single signal generator and vector voltmeter for relatively low band and a heterodyne method to frequency down-mix associated with an additional signal source as well as frequency mixers for high band are used, respectively. Using these two methods, scattering parameters of UHF and X bands are evaluated and their performances are verified through a comercial vector network analyzer.