• Reproductive and Developmental Biology
• /
• 제33권3호
• /
• pp.133-137
• /
• 2009

Pluripotent embryonic stem (ES) cells isolated from inner cell mass (ICM) of blastocyst-stage embryos are capable of differentiating into various cell lineages and demonstrate germ-line transmission in experimentally produced chimeras. These cells have a great potential as tools for transgenic animal production, screening of newly-developed drugs, and cell therapy. Miniature pigs, selectively bred pigs for small size, offer several advantages over large breed pigs in biomedical research including human disease model and xenotransplantation. In the present study, factors affecting primary culture of somatic cell nuclear transfer blastocysts from miniature pigs for isolation of ES cells were investigated. Formation of primary colonies occurred only on STO cells in human ES medium. In contrast, no ICM outgrowth was observed on mouse embryonic fibroblasts (MEF) in porcine ES medium. Plating intact blastocysts and isolated ICM resulted in comparable attachment on feeder layer and primary colony formation. After subculture of ES-like colonies, two putative ES cell lines were isolated. Colonies of putative ES cells morphologically resembled murine ES cells. These cells were maintained in culture up to three passages, but lost by spontaneous differentiation. The present study demonstrates factors involved in the early stage of nuclear transfer ES cell isolation in miniature pigs. However, long-term maintenance and characterization of nuclear transfer ES cells in miniature pigs are remained to be done in further studies.

• Journal of Animal Science and Technology
• /
• 제62권3호
• /
• pp.348-355
• /
• 2020

Cyclophosphamide, a cytotoxic anticancer agent, induces immunosuppression and has several adverse effects. N-acetylcysteine alleviates oxidative stress, liver injury, and intestinal tissue damage. The present study examined whether N-acetylcysteine modulates the adverse effects of cyclophosphamide in pigs. Miniature pigs (n = 15) were used as an experimental model to evaluate the effects of N-acetylcysteine treatment on immune reactions, liver injury, and oxidative stress after cyclophosphamide challenge. Corn-soybean meal based dietary treatments were as follows: control diet with either saline injection, cyclophosphamide injection, or 0.5% N-acetylcysteine and cyclophosphamide injection. N-acetylcysteine increased the number of immune cells and decreased TNF-α production after cyclophosphamide injection and decreased TNF-α, IFN-γ, NF-κB, and IL-8 expression and increased IL-10 expression in peripheral blood mononuclear cells. Serum levels of alanine transaminase and aspartate aminotransferase decreased, superoxide dismutase activity increased, and malondialdehyde activity decreased following N-acetylcysteine treatment after cyclophosphamide injection. N-acetylcysteine decreases immunosuppression, liver injury, and oxidative stress in cyclophosphamide-challenged miniature pigs. The present study suggests that N-acetylcysteine has therapeutic application in livestock for modulating immune reactions, liver injury, and oxidative stress.

• 한국전자통신학회논문지
• /
• 제15권6호
• /
• pp.1055-1060
• /
• 2020

본 연구는 축소형 항공기의 레이다 단면적 분석 및 측정에 대한 연구이다. 사전에 축소형 항공기에 대한 레이다 단면적 특성을 전자기 해석 툴을 사용하여 분석하고 실제 축소형 항공기를 제작하여 무반사실에서 측정하였다. 측정 시 구모델을 사용하여 RCS 특성의 기준 데이터로 적용하여 실제 축소형 모델의 시험 결과 데이터에 적용하였다. 측정 방법은 시간 게이팅을 적용하여 무반사실 내부에서 산란되는 성분에 대한 영향을 제거하여 측정의 정확성을 향상시켰다. 축소형 모델의 RCS 시험 결과는 사전 해석 결과와 같이 초단파 대역에서 상대적으로 RCS 특성이 높게 측정되었다. 향후 본 연구를 통하여 파장이 상대적으로 큰 VHF/UHF 대역 레이다의 표적에 대한 RCS 분석 및 측정에 대한 특성 기술에 활용할 예정이다.

• 대한토목학회논문집
• /
• 제33권2호
• /
• pp.631-642
• /
• 2013

현장지반의 공학적 특성을 파악하기 위한 콘 관입시험(Cone Penetration Test; CPT)은 원지반의 연속적인 강도 특성을 분석하여 다양한 지반변수를 손쉽게 획득할 수 있다는 점에서 널리 활용되고 있으며, 원심모형실험에서도 널리 활용되고 있다. 본 연구에서는 원심모형실험에서 콘선단저항치를 계측할 수 있는 직경이 10 mm인 소형 콘을 개발하고 원심모형실험에서의 적용성을 평가하였다. 개발된 콘으로 4자유도 로봇을 활용하여 원심모형 가속 상태에서 콘 관입시험을 수행하였다. 이 때, 원심가속도 수준을 4회 변화시켜 다양한 유효응력상태에서 콘 관입시험을 실험을 수행하였다. 그 결과, 얕은 관입깊이의 동일한 유효응력에서 콘 선단저항치는 g-level에 영향을 받으며, 선단저항치가 임계 깊이 도달하는 깊이는 g-level과 상대밀도가 커질수록 깊어짐을 확인하였다. 또한, 각 실험에서 임계 깊이에 도달한 선단저항치와 실내실험에서 획득한 지반물성을 이용하여 기존 경험식과 비교하였다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• 제42권1호
• /
• pp.20-30
• /
• 2016

Objectives: To validate a critical-size mandibular bone defect model in miniature pigs. Materials and Methods: Bilateral notch defects were produced in the mandible of dentally mature miniature pigs. The right mandibular defect remained untreated while the left defect received an autograft. Bone healing was evaluated by computed tomography (CT) at 4 and 16 weeks, and by micro-CT and non-decalcified histology at 16 weeks. Results: In both the untreated and autograft treated groups, mineralized tissue volume was reduced significantly at 4 weeks post-surgery, but was comparable to the pre-surgery levels after 16 weeks. After 16 weeks, CT analysis indicated that significantly greater bone was regenerated in the autograft treated defect than in the untreated defect (P=0.013). Regardless of the treatment, the cortical bone was superior to the defect remodeled over 16 weeks to compensate for the notch defect. Conclusion: The presence of considerable bone healing in both treated and untreated groups suggests that this model is inadequate as a critical-size defect. Despite healing and adaptation, the original bone geometry and quality of the pre-injured mandible was not obtained. On the other hand, this model is justified for evaluating accelerated healing and mitigating the bone remodeling response, which are both important considerations for dental implant restorations.

• 한국환경과학회지
• /
• 제23권11호
• /
• pp.1801-1805
• /
• 2014

Longshore current is main transportation mean causing movement of bed load and suspended particle in coastal waters, and effective measurement method and suitable equipment for shallow water coastal environment where is frequently exposed to atmosphere. Measurement equipment for longshore drift was designed and miniature model was applied to Gyeongpo beach in May and June, 2014. The equipment consists of three main elements, spheroid outer casing, spheroid inner casing, observation module equipped with GPS. Gyroscope principle was applied to observation module, and GPS receiver always can be directed upwards. Miniature models were installed along Gyeonpo beach, and it was well to track the flow of longshore current. This research described the design and function of the equipment and results of field experiments.

• 한국초전도ㆍ저온공학회논문지
• /
• 제6권1호
• /
• pp.53-58
• /
• 2004

A Miniature inertance pulse tube refrigerator was designed and tested with a 10 W compressor. Erperiments were carried out for different pulse tube length and inertance tube length. An adiabatic model which considered the pressure drop in the regenerator was used to analyze the performance of the pulse tube refrigerator. Among various design parameters which should be optimized, the pulse tube length and the inertance tube length were optimized. PdV work and several different loss mechanism were included in the analysis to simulate more accurately the physical phenomena in the pulse tube refrigerator. Nevertheless, the simulation program could not completely predict the porformance of the refrigerator. The possible reason for the difference of the optimal point between the simulation and the experiment was explained.

• International Journal of Air-Conditioning and Refrigeration
• /
• 제9권1호
• /
• pp.71-76
• /
• 2001

Factors governing the rate of heat exchange comprise temperature, air velocity, relative humidity, and relation indoors. Recently, there are many researches on the transient analysis of indoor environmental factors such as dry bulb temperature, relative humidity and air velocity in miniature models. The purpose of this study is to measure the environmental factors and to analyze and evaluate the characteristics of indoor environment in different envelop structures using granite dome models. The interior relative humidity is constant regardless of exterior humidity although a little range of variation is shown in comparison to the cement model.

• 한국초전도저온공학회:학술대회논문집
• /
• 한국초전도저온공학회 2003년도 학술대회 논문집
• /
• pp.28-32
• /
• 2003

Miniature inertance pulse tube refrigerator was designed and fabricated to provide optimal performance with 10W compressor. An adiabatic model was used to design an inertance pulse tube refrigerator. Among the various design parameters which should be optimized, pulse tube length and inertance tube length were considered. PdV work and various losses were calculated for various pulse tube and inertance tube length. Experiments were carried out for different pulse tube length and inertance tube length. It was shown that the experimental results were similar to the calculated results. The reason why the quantitative optimal point exists is explained.

• 대한기계학회논문집A
• /
• 제28권12호
• /
• pp.1958-1967
• /
• 2004

This paper describes the method to rapidly fabricate the large-sized physical model with the envelope model size of more than 600 mm${\times}$ 600 mm${\times}$ 600 mm using two type semi-automatic VLM-ST processes in connection with the reverse engineering technology. The fabrication procedure of the large-sized solid shape is as follows: (1) Generation of STL data from 3D scan data using 3D scanner, (2) generation of shell-type STL data by Boolean operation, (3) division of shell-type STL data into several pieces by solid splitting, (4) generation of USL data for each piece with VLM-Slicer, (5) fabrication of each piece by cutting and stacking according to USL data using VLM-ST apparatus, (6) completion of a shell-type prototype by zigzag stacking and assembly for each piece, (7) completion of a 3D solid shape by foam backing, (8) surface finish of a completed 3D solid shape by coating and sanding. In order to examine the applicability of the proposed method, the miniature of the Mount Rushmore Memorial has been fabricated. The envelope model size of the miniature of the Mount Rushmore Memorial is 1,453 mm${\times}$ 760 mm${\times}$ 853 mm in size. From the result of the fabricated miniature of the Mount Rushmore Memorial, it has been shown that the method to fabricate the large object using two type semi-automatic VLM-ST processes in connection with the reverse engineering technology are very fast and efficient.