• 한국토양비료학회지
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• 제44권1호
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• pp.58-66
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• 2011

Arsenic pollution is a serious global concern which affects all life forms. Being a toxic metalloid, the continued search for appropriate technologies for its remediation is needed. Phytoremediation, the use of green plants, is not only a low cost but also an environmentally friendly approach for metal uptake and stabilization. However, its application is limited by slow plant growth which is further aggravated by the phytotoxic effect of the pollutant. Attempts to address these constraints were done by exploiting plant-microbe interactions which offers more advantages for phytoremediation. Several bacterial mechanisms that can increase the efficiency of phytoremediation of As are nitrogen fixation, phosphate solubilization, siderophore production, ACC deaminase activity and growth regulator production. Many have been reported for other metals, but few for arsenic. This mini-review attempts to present what has been done so far in exploring plants and their rhizosphere microbiota and some genetic manipulations to increase the efficiency of arsenic soil phytoremediation.

• Asian Pacific Journal of Cancer Prevention
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• 제15권20호
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• pp.8571-8576
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• 2014

Phytomedicines are believed to have benefits over conventional drugs and are regaining interest in current research. Moringa oleifera is a multi-purpose herbal plant used as human food and an alternative for medicinal purposes worldwide. It has been identified by researchers as a plant with numerous health benefits including nutritional and medicinal advantages. Moringa oleifera contains essential amino acids, carotenoids in leaves, and components with nutraceutical properties, supporting the idea of using this plant as a nutritional supplement or constituent in food preparation. Some nutritional evaluation has been carried out in leaves and stems. An important factor that accounts for the medicinal uses of Moringa oleifera is its very wide range of vital antioxidants, antibiotics and nutrients including vitamins and minerals. Almost all parts from Moringa can be used ad a source for nutrition with other useful values. This mini-review elaborates on details of its health benefits.

• Journal of Plant Biotechnology
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• 제43권1호
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• pp.1-11
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• 2016

Single nucleotide polymorphism (SNP) is an abundant form of genetic variation within individuals of species. DNA polymorphism can arise throughout the whole genome at different frequencies in different species. SNP may cause phenotypic diversity among individuals, such as individuals with different color of plants or fruits, fruit size, ripening, flowering time adaptation, quality of crops, grain yields, or tolerance to various abiotic and biotic factors. SNP may result in changes in amino acids in the exon of a gene (asynonymous). SNP can also be silent (present in coding region but synonymous). It may simply occur in the noncoding regions without having any effect. SNP may influence the promoter activity for gene expression and finally produce functional protein through transcription. Therefore, the identification of functional SNP in genes and analysis of their effects on phenotype may lead to better understanding of their impact on gene function for varietal improvement. In this mini-review, we focused on evidences revealing the role of functional SNPs in genes and their phenotypic effects for the purpose of crop improvements.

• 공업화학
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• 제30권2호
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• pp.141-144
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• 2019

물 재이용을 위한 오일 폐수 처리 기술은 일반적으로 물과 오일을 분리하고자 하는 유수분리장치를 이용하는데, 최근 다양한 표면 개질 방법을 이용하여 기능성이 향상된 유수분리막을 기반으로 유수분리장치를 구현하고자 하는 연구개발이 지속적으로 이어지고 있다. 분리막 소재 기술은 분리 선택성 및 내구성 등 성능뿐만 아니라 경제성 및 가공성등 상용화를 위한 적용성이 검토되어야 하며, 소재의 최적 성능을 최대한 발휘될 수 있도록 효과적인 장치가 구비되어야 한다. 본 논문에서는 분리막 소재의 상용 수준의 대면적 가공성 및 효과적인 유수분리 장치에 대해서 고찰하고자 한다.

• 한국토양비료학회지
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• 제49권1호
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• pp.17-29
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• 2016

Though there is an abundant supply of nitrogen in the atmosphere, it cannot be used directly by the biological systems since it has to be combined with the element hydrogen before their incorporation. This process of nitrogen fixation ($N_2$-fixation) may be accomplished either chemically or biologically. Between the two elements, biological nitrogen fixation (BNF) is a microbiological process that converts atmospheric di-nitrogen ($N_2$) into plant-usable form. In this review, the genetics and mechanism of nitrogen fixation including genes responsible for it, their types and role in BNF are discussed in detail. Nitrogen fixation in the different agricultural systems using different methods is discussed to understand the actual rather than the potential $N_2$-fixation procedure. The mechanism by which the diazotrophic bacteria improve plant growth apart from nitrogen fixation such as inhibition of plant ethylene synthesis, improvement of nutrient uptake, stress tolerance enhancement, solubilization of inorganic phosphate and mineralization of organic phosphate is also discussed. Role of diazotrophic bacteria in the enhancement of nitrogen fixation is also dealt with suitable examples. This mini review attempts to address the importance of diazotrophic bacteria in nitrogen fixation and plant growth improvement.

• The Plant Pathology Journal
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• 제35권4호
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• pp.351-361
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• 2019

In our previous study, pyrrolnitrin produced in Pseudomonas chlororaphis G05 plays more critical role in suppression of mycelial growth of some fungal pathogens that cause plant diseases in agriculture. Although some regulators for pyrrolnitrin biosynthesis were identified, the pyrrolnitrin regulation pathway was not fully constructed. During our screening novel regulator candidates, we obtained a white conjugant G05W02 while transposon mutagenesis was carried out between a fusion mutant $G05{\Delta}phz{\Delta}prn::lacZ$ and E. coli S17-1 (pUT/mini-Tn5Kan). By cloning and sequencing of the transposon-flanking DNA fragment, we found that a vfr gene in the conjugant G05W02 was disrupted with mini-Tn5Kan. In one other previous study on P. fluorescens, however, it was reported that the deletion of the vfr caused increased production of pyrrolnitrin and other antifungal metabolites. To confirm its regulatory function, we constructed the vfr-knockout mutant $G05{\Delta}vfr$ and $G05{\Delta}phz{\Delta}prn::lacZ{\Delta}vfr$. By quantifying ${\beta}-galactosidase$ activities, we found that deletion of the vfr decreased the prn operon expression dramatically. Meanwhile, by quantifying pyrrolnitrin production in the mutant $G05{\Delta}vfr$, we found that deficiency of the Vfr caused decreased pyrrolnitrin production. However, production of phenazine-1-carboxylic acid was same to that in the wild-type strain G05. Taken together, Vfr is required for pyrrolnitrin but not for phenazine-1-carboxylic acid biosynthesis in P. chlororaphis G05.

• The Plant Pathology Journal
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• 제24권4호
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• pp.453-460
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• 2008

In the course of in vitro and in vivo screening for bioherbicidal agents, a hyphomycete fungus, Myrothecium sp. F0252 was selected as a candidate for the biocontrol of weeds. The isolate was identified as Myrothecium roridum Tode ex. Fries based on the morphological characteristics and 18S ribosomal DNA sequence analysis and registered as Myrothecium roridum F0252. In order to evaluate the in vitro effect of M. roridum F0252 on germination of ladino clover and white clover (Trifolium repens L.) seeds, spore solution of the fungus was employed in two concentrations, $6.5{\times}10^6$ and $2.5{\times}10^7$ spores per mL and then inoculated to the seeds. The fungal spores inhibited the seed germination, infected the seedlings, and caused an abnormal withering and inhibition of seedling growth. In addition, when the herbicidal activity of crude ethyl acetate extract from the liquid culture was assessed on a mini-plant, duck-weed (Lemna paucicostata (L.) Hegelm.), the extract showed high inhibitory effect at the level of $12.5{\mu}g$ per mL. On the other hand, in vivo herbicidal activity of M. roridum F0252 was evaluated by a whole plant spray method. M. roridum F0252 exhibited strong and broad-spectrum herbicidal activity. The herbicidal values ranged from 95-100% against 7 weeds, including Abutilon avicennae and Xanthium strumarium, and 70-80% against Digitaria sanguinalis and Sagittaria pygmaea. When the nutritional utilization (95 carbon sources) pattern of M. roridum F0252 was investigated, it varied with water activity ($a_w$) and temperature conditions, supplying good, basic information in regard to nutritional utilization for proper cultivation and formulation. Our results showed that M. roridum F0252 might be used as a potential biocontrol agent against weedy plants.

• 대한환경공학회지
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• 제28권1호
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• pp.97-103
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• 2006

본 논문은 미세 오존 출력을 얻을 수 있는 자동제어형 오존발생장치의 제어기법을 제시하고, 또한 무성방전에 의한 오존발생장치의 유입공기를 공극에서 저온인 $2^{\circ}C$까지 냉각 하여 포화 확산도가 높은 고밀도 탈취용 오존을 발생시킬 수 있는 기법을 제시한다. 고주파수의 오존방전 노이즈에 의한 전원파형의 불량화를 보상하기 위해서 디지털 궤환 제어시스템을 구성하고, 방전관의 등가 커패시터의 전압과 전류를 검출하여 2중의 제어루프를 설계한다. 부하변동에 따르는 과도상태 응답특성을 개선하고 제어기의 연산 지연시간을 보상하기 위하여 연산시간을 전원장치 플랜트의 고유한 파라미터로 가정하여 플랜트모델에 포함시켜 모델링한다. 제안된 오존 발생기는 고농도 고효율의 오존방전 시스템의 입력전원에서 고주파방전노이즈가 제거되어 정현적으로 되며 2배 이상의 안정된 오존출력을 얻는 것을 실험적으로 입증한다.

• 생물환경조절학회지
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• 제6권1호
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• pp.34-42
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• 1997

미니 토마토 ‘미니 캐롤’ 품종의 플러그 육묘시 주야간 온도조합 처리에 의한 유묘의 절간신장 및 정식후 화아분화에 미치는 영향을 조사하였다. 유묘의 초장신장에는 주간온도가 야간온도보다 크게 영향을 미쳤으며, 특히 같은 일평균기온에서 +DIF가 -DIF보다 절간신장에 영향이 더 큰 것으로 나타났다. 엽전개속도는 일평균기온이 증가할수록 빨라지는 경향이었으며, 묘의 충실도(건물중/초장) 지수는 일평균기온이 증가할수록 저하되었다. 제 1화방의 착생절위는 일평균기온이 16$^{\circ}C$에서는 정상적으로 화아분화 되었으나 일평균기온이 증가하면서 제 1화방 착생절위도 증가하여 28$^{\circ}C$에서는 16$^{\circ}C$에 비해 약 4마디가 지연되었다. 정식후 절간신장은 육묘시 절간장과는 상관없이 각 처리간 차이가 인정되지 않았다. 그러나 마디수는 정식전의 마디수의 차이 그대로 유지되었다. 육묘시 -DIF 조건에서 초장이 억제된 묘라 할지라도 정식후 정상적인 생장반응을 나타내었으며, 제 1화방의 착생절위는 일평균기온(16$^{\circ}C$)이 낮을수록 저절위(제9절)가 되는 것으로 밝혀졌다.

• 원예과학기술지
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• 제29권1호
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• pp.77-80
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• 2011

경남농업기술원 화훼연구소에서 황색 대륜계 '99-가-2'를 모본, 복색 중륜계 '99-나-3-1'을 부본으로 인공교배를 실시하여 복색 소륜계 거베라 'Sun Cap'을 육성하였다. 특성 검정 포장에 정식하여 2004년부터 2007년까지 3회에 걸쳐 생육 및 수량특성 검정을 실시하였다. 'Sun Cap'은 선명한적/황의 복색(RHS 45-B/9-A)과 녹색의 화심이 잘 조화되는 화경이 강건하고 안정된 화형의 홑꽃 소륜화이다. 년간 주당평균수량은 약 102.6본 정도이며, 평균 절화수명은 약 13.5일 정도이다.